• Korean Journal of Acupuncture
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• v.39 no.2
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• pp.54-62
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• 2022

Objectives : The purpose of this study was to evaluate the potential of the test substance, SU-Eohyeol Pharmacopuncture (SUEP), to induce micronuclei in bone marrow cells of Sprague-Dawley (SD) Rats. Methods : The dose range preliminary study was performed first. 1 ml/animal was selected as the high dose of this study. Two additional lower dose levels (0.5 and 0.25 ml/animal) were produced by applying a geometric ratio of 2. In addition, the positive and negative control groups were set. Then, after intramuscular administration (1 ml/animal) of SUEP to 8-week-old male SD rats, an in vivo micronucleus test was performed to evaluate the induction of micronuclei in SD rat bone marrow cells. Results : As a result of the main study, the incidence of micronucleated polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE) in the test substance SUEP groups was not statistically significantly different from the negative control group. In addition, the ratio of PCE to total erythrocytes in the test substance SUEP groups was not statistically significantly different from the negative control group. In the positive control group, the incidence of MNPCE in PCE was statistically significantly increased when compared to the negative control group. The ratio of PCE to total erythrocytes in the positive control group was not statistically significantly different from the negative control group. Conclusions : Based on these results, the test substance, SUEP, did not have any potential to induce micronuclei formation in bone marrow cells of rats under the conditions of this study.

• Journal of Veterinary Science
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• v.23 no.1
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• pp.14.1-14.11
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• 2022

Background: Carnivore protoparvovirus 1, also known as canine parvovirus type 2 (CPV-2), is the main pathogen in hemorrhagic gastroenteritis in dogs, with a high mortality rate. Three subtypes (a, b, c) have been described based on VP2 residue 426, where 2a, 2b, and 2c have asparagine, aspartic acid, and glutamic acid, respectively. Objectives: This study examined the presence of CPV-2 variants in the fecal samples of dogs diagnosed with canine parvovirus in Bogotá. Methods: Fecal samples were collected from 54 puppies and young dogs (< 1 year) that tested positive for the CPV through rapid antigen test detection between 2014-2018. Molecular screening was developed for VP1 because primers 555 for VP2 do not amplify, it was necessary to design a primer set for VP2 amplification of 982 nt. All samples that were amplified were sequenced by Sanger. Phylogenetics and structural analysis was carried out, focusing on residue 426. Results: As a result 47 out of 54 samples tested positive for VP1 screening, and 34/47 samples tested positive for VP2 980 primers as subtype 2a (n = 30) or 2b (n = 4); subtype 2c was not detected. All VP2 sequences had the amino acid, T, at 440, and most Colombian sequences showed an S514A substitution, which in the structural modeling is located in an antigenic region, together with the 426 residue. Conclusions: The 2c variant was not detected, and these findings suggest that Colombian strains of CPV-2 might be under an antigenic drift.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.41-76
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• 2001

Rheumatoid arthritis is an incurable chronic inflammatory and destructive arthopathy that affects 1% of the population world-wide. It has substantial personal, social and economic costs. The long-term prognosis is poor: 80 percent of affected patients will become disabled within 20 years after onset of disease. Medical costs of rheumatoid arthritis average ∼$ 6000 (US) per patient (1), Current antirheumatic drugs have limited efficacy and many side effects and more importantly they do not improve the long-term prognosis of rheumatoid arthritis (2). After a decade of few notable advances in therapy, several biological response modifiers that target pathophysiological processes in the disease have now emerged in the clinic. These new drugs are termed biological agents, and although information about their use in the clinic is still limited to short term treatment, they appear to have the ability to modify disease progress. In addition, COX-2 selective agents have now been approved that have comparable efficacy with standard NSAIDs, but fewer gastrointestinal side effects (3). Thus today many more therapeutic options are suddenly open to patients that even five years ago had little hope of relief from chronic pain and inflammation.

• Journal of Veterinary Science
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• v.24 no.3
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• pp.31.1-31.6
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• 2023

Allograft arthroplasty is a promising cartilage-resurfacing technique. A 14-year-old horse was diagnosed with a medial femoral condyle subchondral bone cyst. Allografts were harvested from a young donor animal and implanted to fill the cyst cavity. A visual assessment of the surgical site was possible during follow-up arthroscopy. In addition to a desirable gliding surface and a good adaptation of the grafts, fibrillation of the cranial ligament of the medial meniscus was noted and debrided. The recipient horse became sound four months after follow-up surgery with a high level of owner satisfaction. In conclusion allograft transplantation is a promising technique for treating subchondral bone cysts.

• Food Science of Animal Resources
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• v.31 no.1
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• pp.54-60
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• 2011

This study was performed to examine the possibility of water extracts of several digestive medicinal plants (DMPEs), such as Amomum tasoko, Alpinia oxyphylla, Citrus unshiu, and Myristica fragrans, as a natural antioxidant. Total phenol contents of each extract were expressed as gallic acid equibalents (GAE) and those were significantly different among A. tasoko ($39.87{\pm}5.77$ mg GAE/g), A. oxyphylla ($30.28{\pm}3.36$ mg GAE/g), C. unshiu ($28.13{\pm}5.01$ mg GAE/g) and M. fragrans ($6.36{\pm}0.30$ mg GAE/g) (p<0.05), and extract of A. tasoko showed significantly higher antioxidative effect than butylated hydroxyanisole (BHA) on linoleic acid peroxidation at 72 h after incubation (p<0.05). Addition of extracts in pork patties did not affect the pH value and total microbes during cold storage. However, thiobarbituric acid reative substances (TBARS) of treated patties were lower in dose dependant manner than that of control as storage period increased (except patties treated with C. unshiu extract), and patties treated with 0.5% A. tasoko extract showed no significant difference with patties treated with 0.5% BHA at day 7.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.301-309
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• 1997

The objective of this study was to determine the microtubule assembly and chromatin configuration during the first cell cycle in bovine oocytes following injection of spermatozoon, sperm head and tail. The microtubule and chromatin configuration was imaged with fluorescent labeled monoclonal ${\alpha}$-tubulin antibody and propidium iodide under laser scanning confocal microscope. Microtubule and chromatin dynamics in bovine oocytes following intracytoplasmic sperm injection (ICSI) were not different from those observed during in vitro fertilization (IVF). Following ICSI, the microtubular aster was observed around sperm midpiece. During pronuclear formation, the sperm aster was enlarged and seen around male and female pronuclei. At mitotic metaphase, the microtubular spindle assemble astral poles and chromosomes were aligned on the spindle equator. At mitosis, asters were concentrated to each spindle pole and they filled the cytoplasm. After injection of the isolated sperm head, the microtubular aster was not seen around sperm head in any cases (0/18). Instead, microtubules were organized from the cytoplasm, which filled the whole cytoplasm during pronuclear apposition. These microtubules seem to move male and female pronuclei. These results suggest that isolated sperm head can develop into normal pronucleus in mature bovine oocytes, and competent to participate syngamy with the ootid chromatin. The functional microtubules following isolated sperm head injection in bovine oocytes appeared to be organized solely from maternal stores.

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.229-237
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• 1999

The influence of ascorbic acid (Asc) and ferrous sulfate (Fe$^{2＋}$) on capacitation, acrosome reaction and fertility in vitro was investigated in boar frozen-thawed spermatozoa with or without preincubation. The addition of 0-1.0 mM Fe$^{2＋}$ to sperm suspensions during preincubation increase acrosome reaction (P＜0.05) and oocyte penetration. These increase are also associated with addition of 0~0.5 mM Asc, but the penetration rates were higher in those without than with sperm preincubation. The addition of 0.1 mM Asc than 0.5 mM in medium with Fe$^{2＋}$ were significantly (P＜0.05) higher on acrosome reaction at 2 h after sperm preincubation. No significant differences, however, were observed in penetration rates among the concentrations of Asc. On the other hand, when preincubation medium containing the Asc was supplemented with 0.1mM Fe$^{2＋}$, the percentage of spermatozoa acrosome-reacted were significantly (P＜0.05) higher than in medium wothout Fe$^{2＋}$, on the contrary, the penetration rate was significantly (P＜0.05) low during in-vitro fertilization. These findings indicate some apparent effects of Fe$^{2＋}$ or Asc addition on acrosome reaction and the fertilizing potential by sperm preincubation.

• Journal of Veterinary Clinics
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• v.37 no.2
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• pp.91-95
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• 2020

A 13-year-old, intact male, Yorkshire terrier dog with oliguria and hematuria was presented to the Small Animal Clinic at the Gyeongsang National University animal medical center. There were no remarkable findings on serum chemistry values and complete blood counts. Abdominal radiographs revealed mild elevation of the descending colon at the pelvic level. On the excretory urographic images, a large filling defect was identified in urinary bladder with narrow urinary bladder wall contact and there were no remarkable findings in kidneys and ureters. The abdominal ultrasonography revealed that an irregularly marginated, heterogeneous mass in urinary bladder, central cavitary lesion and mild vascular flow were also observed. Computed tomography showed a heterogeneously attenuated mass within urinary bladder. The mass was surgically excised, and leiomyosarcoma was confirmed by histopathological examination. The patient was followed up for two months and there were no complications. This case is of interest because of the unusual findings of leiomyosarcoma of urinary bladder in a small breed dog.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.428-438
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• 1999

A study was conducted to estimate cost of major dairy cattle diseases. Forty (n=40) of the 167 dairy herds in Gyeongnam (Chinju) area were stratified and selected randomly for participation in the national animal health monitoring system. Gyeongsnag University veterinarians, Gyeongnam Livestock Promotion Institute veterinarians and clinic veterinarian visited each herd once a month for a total periods of 12 months. At a each visit data on disease, production, management, finance, treatments, preventive activities, animal events, and any other relevant events were collected. Monthly and annual cost estimates of disease treatment were in computed in each herd and stratum(including cost of prevention). Results were expressed as cost per head and given separately for cows, young stock, and calves. In cows, the most expensive seven diseases entities (from the most to the least) were : (1) clinical mastitis; (2) breeding problems; (3)gastrointestinal problems; (4) multiple system problem; (5) birth problems; (6) metabolic/nutritional disease; (7) lameness. In young stock, the most costly disease were the multiple system problems, breeding problems, respiratory disease, gastrointestinal disease, and lameness. In calves, the most costly disease problems were gastrointestinal problems, respiratory disease, integumental, multiple system problems, and metabolic/nutritional problems.

• Journal of Veterinary Clinics
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• v.27 no.1
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• pp.83-87
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• 2010

Hermaphroditism occurs rarely in human and animal populations. Previously, in Korea, The hermaphrodite dog in American Cocker Spaniel dogs have been reported in six cases. However, in this case, we found a hermaphrodite dog in Korean native Jindo dog. The characteristics were its enlarged os clitoris that was shown a reddish fingerlike structure protruding from the vulva. The dog was diagnosed as hermaphroditism with ovotestes. To our knowledge, this report is the first case of hermaphroditism in Korean native dog.