Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular diversity of the VP2 of Carnivore protoparvovirus 1 (CPV-2) of fecal samples from Bogotá

  • Galvis, Cristian Camilo (College of Veterinary Medicine, Antonio Narino University) ;
  • Jimenez-Villegas, Tatiana (Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of Sao Paulo) ;
  • Romero, Diana Patricia Reyes (Vida Animal Veterinary Services) ;
  • Velandia, Alejandro (Clinivet Veterinary Clinic) ;
  • Taniwaki, Sueli (Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of Sao Paulo) ;
  • Silva, Sheila Oliveira de Souza (Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of Sao Paulo) ;
  • Brandao, Paulo (Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of Sao Paulo) ;
  • Santana-Clavijo, Nelson Fernando (Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of Sao Paulo)
  • Received : 2021.06.24
  • Accepted : 2021.09.16
  • Published : 2022.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Carnivore protoparvovirus 1, also known as canine parvovirus type 2 (CPV-2), is the main pathogen in hemorrhagic gastroenteritis in dogs, with a high mortality rate. Three subtypes (a, b, c) have been described based on VP2 residue 426, where 2a, 2b, and 2c have asparagine, aspartic acid, and glutamic acid, respectively. Objectives: This study examined the presence of CPV-2 variants in the fecal samples of dogs diagnosed with canine parvovirus in Bogotá. Methods: Fecal samples were collected from 54 puppies and young dogs (< 1 year) that tested positive for the CPV through rapid antigen test detection between 2014-2018. Molecular screening was developed for VP1 because primers 555 for VP2 do not amplify, it was necessary to design a primer set for VP2 amplification of 982 nt. All samples that were amplified were sequenced by Sanger. Phylogenetics and structural analysis was carried out, focusing on residue 426. Results: As a result 47 out of 54 samples tested positive for VP1 screening, and 34/47 samples tested positive for VP2 980 primers as subtype 2a (n = 30) or 2b (n = 4); subtype 2c was not detected. All VP2 sequences had the amino acid, T, at 440, and most Colombian sequences showed an S514A substitution, which in the structural modeling is located in an antigenic region, together with the 426 residue. Conclusions: The 2c variant was not detected, and these findings suggest that Colombian strains of CPV-2 might be under an antigenic drift.

Keywords

Acknowledgement

This work was funded by CNPq (Brazilian National Board for Scientific and Technological Development) grant numbers 307291/2017-0 and 400604/2016-7 and CAPES (Coordination for the Improvement of Higher Education Personnel, Brazil) Finance Code 001, which had no role in the study design, collection, analysis, and interpretation of data, writing of the report or decision to submit the article for publication.

References

  1. Cotmore SF, Agbandje-McKenna M, Chiorini JA, Mukha DV, Pintel DJ, Qiu J, et al. The family Parvoviridae. Arch Virol. 2014;159(5):1239-1247. https://doi.org/10.1007/s00705-013-1914-1
  2. Reed AP, Jones EV, Miller TJ. Nucleotide sequence and genome organization of canine parvovirus. J Virol. 1988;62(1):266-276. https://doi.org/10.1128/jvi.62.1.266-276.1988
  3. Wang J, Lin P, Zhao H, Cheng Y, Jiang Z, Zhu H, et al. Continuing evolution of canine parvovirus in China: isolation of novel variants with an Ala5Gly mutation in the VP2 protein. Infect Genet Evol. 2016;38:73-78. https://doi.org/10.1016/j.meegid.2015.12.009
  4. Lamm CG, Rezabek GB. Parvovirus infection in domestic companion animals. Vet Clin North Am Small Anim Pract. 2008;38(4):837-850. https://doi.org/10.1016/j.cvsm.2008.03.008
  5. Truyen U, Parrish CR, Harder TC, Kaaden OR. There is nothing permanent except change. The emergence of new virus diseases. Vet Microbiol. 1995;43(2-3):103-122. https://doi.org/10.1016/0378-1135(95)92531-F
  6. Buonavoglia D, Cavalli A, Pratelli A, Martella V, Greco G, Tempesta M, et al. Antigenic analysis of canine parvovirus strains isolated in Italy. New Microbiol. 2000;23(1):93-96.
  7. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, et al. Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol. 2001;82(Pt 12):3021-3025. https://doi.org/10.1099/0022-1317-82-12-3021
  8. Decaro N, Buonavoglia C. Canine parvovirus--a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol. 2012;155(1):1-12. https://doi.org/10.1016/j.vetmic.2011.09.007
  9. Zhou P, Zeng W, Zhang X, Li S. The genetic evolution of canine parvovirus - a new perspective. PLoS One. 2017;12(3):e0175035. https://doi.org/10.1371/journal.pone.0175035
  10. Mogollon J, Cortes E, Benavides O, Forero G. Parvovirus Canina en Colombia. ACOVEZ. 1981;5(18):201-207.
  11. Castillo A, Diez H, Almanza J, Jerabek L, Torres O. Analisis genomico de parvovirus canino por PCR-RFLP a partir de aislamientos de casos clinicos sintomaticos tomados en Bogota-Colombia. Univ Sci. 2001;6(2):49-53.
  12. Duque-Garcia Y, Echeverri-Zuluaga M, Trejos-Suarez J, Ruiz-Saenz J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: a possible new CPV-2a is emerging? Vet Microbiol. 2017;201:56-61. https://doi.org/10.1016/j.vetmic.2016.12.039
  13. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 1993;15(3):532-4, 536-7.
  14. Leutenegger CM, Mislin CN, Sigrist B, Ehrengruber MU, Hofmann-Lehmann R, Lutz H. Quantitative real-time PCR for the measurement of feline cytokine mRNA. Vet Immunol Immunopathol. 1999;71(3-4):291-305. https://doi.org/10.1016/S0165-2427(99)00100-2
  15. Demeter Z, Gal J, Palade EA, Rusvai M. Feline parvovirus infection in an Asian palm civet (Paradoxurus hermaphroditus). Vet Rec. 2009;164(7):213-216. https://doi.org/10.1136/vr.164.7.213
  16. Furtado MM, Taniwaki SA, de Barros IN, Brandao PE, Catao-Dias JL, Cavalcanti S, et al. Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil. J Vet Diagn Invest. 2017;29(5):660-668. https://doi.org/10.1177/1040638717720245
  17. Pinto LD, Barros IN, Budaszewski RF, Weber MN, Mata H, Antunes JR, et al. Characterization of pantropic canine coronavirus from Brazil. Vet J. 2014;202(3):659-662. https://doi.org/10.1016/j.tvjl.2014.09.006
  18. Hall TA. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser (Oxf). 1999;41:95-98.
  19. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  20. Bertoni M, Kiefer F, Biasini M, Bordoli L, Schwede T. Modeling protein quaternary structure of homoand hetero-oligomers beyond binary interactions by homology. Sci Rep. 2017;7(1):10480. https://doi.org/10.1038/s41598-017-09654-8
  21. Wu H, Rossmann MG. The canine parvovirus empty capsid structure. J Mol Biol. 1993;233(2):231-244. https://doi.org/10.1006/jmbi.1993.1502
  22. Mittal M, Chakravarti S, Mohapatra JK, Chug PK, Dubey R, Upmanuyu V, et al. Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure. Infect Genet Evol. 2014;23:1-6. https://doi.org/10.1016/j.meegid.2014.01.015
  23. Day MJ, Horzinek MC, Schultz RD, Squires RAVaccination Guidelines Group (VGG) of the World Small Animal Veterinary Association (WSAVA). WSAVA Guidelines for the vaccination of dogs and cats. J Small Anim Pract. 2016;57(1):E1-E45. https://doi.org/10.1111/jsap.2_12431
  24. Aldaz J, Garcia-Diaz J, Calleros L, Sosa K, Iraola G, Marandino A, et al. High local genetic diversity of canine parvovirus from Ecuador. Vet Microbiol. 2013;166(1-2):214-219. https://doi.org/10.1016/j.vetmic.2013.06.012
  25. Zourkas E, Ward MP, Kelman M. Canine parvovirus in Australia: a comparative study of reported rural and urban cases. Vet Microbiol. 2015;181(3-4):198-203. https://doi.org/10.1016/j.vetmic.2015.10.009
  26. Giraldo-Ramirez S, Rendon-Marin S, Ruiz-Saenz J. Phylogenetic, evolutionary and structural analysis of canine parvovirus (CPV-2) antigenic variants circulating in Colombia. Viruses. 2020;12(5):500. https://doi.org/10.3390/v12050500
  27. Decaro N, Desario C, Parisi A, Martella V, Lorusso A, Miccolupo A, et al. Genetic analysis of canine parvovirus type 2c. Virology. 2009;385(1):5-10. https://doi.org/10.1016/j.virol.2008.12.016
  28. Mira F, Dowgier G, Purpari G, Vicari D, Di Bella S, Macaluso G, et al. Molecular typing of a novel canine parvovirus type 2a mutant circulating in Italy. Infect Genet Evol. 2018;61:67-73. https://doi.org/10.1016/j.meegid.2018.03.010
  29. Decaro N, Buonavoglia C, Barrs VR. Canine parvovirus vaccination and immunisation failures: are we far from disease eradication? Vet Microbiol. 2020;247:108760. https://doi.org/10.1016/j.vetmic.2020.108760
  30. Chung HC, Kim SJ, Nguyen VG, Shin S, Kim JY, Lim SK, et al. New genotype classification and molecular characterization of canine and feline parvoviruses. J Vet Sci. 2020;21(3):e43. https://doi.org/10.4142/jvs.2020.21.e43
  31. Hong C, Decaro N, Desario C, Tanner P, Pardo MC, Sanchez S, et al. Occurrence of canine parvovirus type 2c in the United States. J Vet Diagn Invest. 2007;19(5):535-539. https://doi.org/10.1177/104063870701900512
  32. Battilani M, Ciulli S, Tisato E, Prosperi S. Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy. Virus Res. 2002;83(1-2):149-157. https://doi.org/10.1016/S0168-1702(01)00431-2
  33. Geng Y, Guo D, Li C, Wang E, Wei S, Wang Z, et al. Co-circulation of the rare CPV-2c with unique Gln370Arg substitution, new CPV-2b with unique Thr440Ala substitution, and new CPV-2a with high prevalence and variation in Heilongjiang Province, Northeast China. PLoS One. 2015;10(9):e0137288. https://doi.org/10.1371/journal.pone.0137288
  34. De la Torre D, Mafla E, Puga B, Erazo L, Astolfi-Ferreira C, Ferreira AP. Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador. Vet World. 2018;11(4):480-487. https://doi.org/10.14202/vetworld.2018.480-487
  35. Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H, et al. The three-dimensional structure of canine parvovirus and its functional implications. Science. 1991;251(5000):1456-1464. https://doi.org/10.1126/science.2006420
  36. Voorhees IE, Lee H, Allison AB, Lopez-Astacio R, Goodman LB, Oyesola OO, et al. Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread. J Virol. 2019;94(1):e01162-e19.
  37. Agbandje M, McKenna R, Rossmann MG, Strassheim ML, Parrish CR. Structure determination of feline panleukopenia virus empty particles. Proteins. 1993;16(2):155-171. https://doi.org/10.1002/prot.340160204
  38. Callaway HM, Feng KH, Lee DW, Allison AB, Pinard M, McKenna R, et al. Parvovirus capsid structures required for infection: mutations controlling receptor recognition and protease cleavages. J Virol. 2017;91(2):e01871-16.
  39. Agbandje M, Parrish CR, Rossmann MG. The structure of parvoviruses. Semin Virol. 1995;6(5):299-309. https://doi.org/10.1006/smvy.1995.0036
  40. Truyen U. Evolution of canine parvovirus--a need for new vaccines? Vet Microbiol. 2006;117(1):9-13. https://doi.org/10.1016/j.vetmic.2006.04.003
  41. Nandi S, Kumar M. Canine parvovirus: current perspective. Indian J Virol. 2010;21(1):31-44. https://doi.org/10.1007/s13337-010-0007-y