• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.356-368
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• 2003

Water quality of effluent from wastewater treatment plants (WWTPS) was reviewed to examine the feasibility of agricultural reuse using USEPA and WHO guidelines. It might meet the guidelines for BOD and SS, however, the most critical microbiological concentration was too high and further treatment is required. The pilot study of three treatments were performed to reduce microbiological concentrations. The UV irradiation was proved to be very effective in disinfection of secondary level effluent, and about 30 mW ${\cdot}$ s/$cm^2$ of dose was suggested to meet the even most stringent USEPA guidelines. Slow sand filter demonstrated effective removal of bacteria, and effluent concentration of total coliform (TC), fecal coliform (FC), and E. coli. dropped from about 10,000/100 mL to 300, 200, and 150 MPN/100 mL, respectively, showing over 95% removal. These level of bacterial concentration sufficiently meet the WHO guidelines ($10^3\;{\sim}\;10^5$ FC/100 mL), and could meet the more stringent USEPA guidelines (200 FC/100 mL) if properly applied. Slow sand filter also provided about 50% removal of SS, turbidity, and BOD in addition to bacterial removal. The removal efficiency of pond system was relatively poor, but still showed over 85% removal and effluent concentration of TC, FC, and E. coli was all below 10,000/100 mL. The pond system alone could meet the WHO guidelines, but hardly meet the USEPA guidelines and further treatment might be necessary. Overall, three methods evaluated in the study treat the effluent to meet the WHO microbiological guidelines for agricultural reuse. The UV disinfection and slow sand filter might also could the USEPA guidelines, while the pond system can hardly meet the USEPA guidelines if applied alone. The WHO and USEPA guidelines were made based on data from upland field agricultural system and may not be directly applicable to the paddy field agricultural system. Therefore, national standards for agricultural reuse of reclaimed water should be made considering domestic agricultural systems as well as international guidelines. Also, further investigation is recommended to develop optimum and feasible treatment measures for agricultural reuse of effluent from WWTPs.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.1
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• pp.71-79
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• 1999

As part of an on-going project investigating flux of materials in the Keum River Estuary, we have monitored seasonal variations of nutrients, suspended particulate matter (SPM), chlorophyll, and salinity since 1997. Meteorological data and freshwater discharge from the Keum River Dike were also used, Our goal was to answers for (1) what is the main factor for the seasonal fluctuation of nutrients in the Keum River Estuary? and (2) are there any differences in nutrient distributions before and after the Keum River Dike construction? Nitrate concentrations in the Keum River water were kept constant through the year. Whereas other nutrients varied with evident seasonality: high phosphate and ammonium concentrations during the dry season and enhanced silicate contents during the rainy season. SPM was found similar trend with silicate. During the rainy season, the freshwater discharged from the Keum River Dike seemed to dilute the phosphate and ammonium, but to elevate SPM concentration in the Keum Estuary. In addition, the corresponding variations of SPM contents in the estuarine water affected the seasonal fluctuations of nutrients in the Estuary. The most important source of the nutrients in the estuarine water is the fluvial water. Therefore, the distribution patterns of nutrients in the Estuary are conservative against salinity. Nitrate, nitrite and silicate are conservative through the year. The distribution of phosphate and ammonium on the other hand, display two distinct seasonal patterns: conservative behavior during the dry season and some additive processes during the rainy days. Mass destruction of freshwater phytoplankton in the riverine water is believed to be a major additive source of phosphate in the upper Estuary. Desorption processes of phosphate and ammonium from SPM and organic matter probably contribute extra source of addition. Benthic flux of phosphate and ammonium from the sediment into overlying estuarine water can not be excluded as another source. After the Keum River Dike construction, the concentrations of SPM decreased markedly and their role in controlling of nutrient concentrations in the Estuary has probably diminished. We found low salinity (5~15 psu) within 1 km away from the Dike during the dry season. Therefore we conclude that the only limited area of inner estuary function as a real estuary and the rest part rather be like a bay during the dry season. However, during the rainy season, the entire estuary as the mixing place of freshwater and seawater. Compared to the environmental conditions of the Estuary before the Dike construction, tidal current velocity and turbidity are decreased, but nutrient concentrations and chance of massive algal bloom such as red tide outbreak markedly increased.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.3
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• pp.151-164
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• 2010

Field observations on the seasonal variations of environmental factors and phytoplankton community were carried out four times at 30 stations in the narrow strait between Yeosu and Dolsan Island of the Korean South Sea from September 2005 to May 2006. The ranges of water temperature, salinity and extinction coefficient in the surface waters were 5.6~26.3, 25.36~33.92 psu and 0.13~2.13, respectively. The water temperature measured higher at Gamak Bay in summer and spring. It measured higher at Yeosuhae Bay in autumn and winter. Salinity showed uniformity of distributions in almost all areas, except for an area near a sewage disposal outlet. Extinction coefficient indicated that the turbidity of Gamak Bay and the area near the sewage disposal outlet were higher than that of the Yeosuhae Bay. In the phytoplankton community were identified a total of 99 species belonging to 51 genera. The species composition showed itself to be various in summer and autumn, but poor in winter and spring with a high ratio of centric diatoms all the year round. Seasonal succession of dominant species were Skeletonema costatum and Chaetoceros curvisetus in summer, Eucampia zodiacus in autumn and winter, and Chaetoceros affinis and Thalassionema nitzschioides inspring. Standing crops of phytoplankton and Chlorophyll $\alpha$ concentration were greatly higher at Gamak Bay in summer with ranges of $0.2{\times}10^4\;cells\;L^{-1}$ to $296{\times}10^4\;L^{-1}$, and $1.94\;L^{-1}$ to $22.12\;L^{-1}$, respectively. From the results of principal component analysis (PCA), the northern part of Dolsan Island was divided into two or three regions from the characteristics of marine environment and phytoplankton community.

• Food Science and Preservation
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• v.12 no.4
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• pp.379-386
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• 2005

Free amino acids and polysaccharide content of submerged mycelial culture of Fomitopsis pinicola using $2\%$ citrus peel water extracts(CP), $2\%$ green tea water extracts(GT) and yeast-malt broth(YM) were investigated. Cultivations were conducted at $30^{\circ}C$ and 150 rpm for 10 days. Yields of the mycelium as fresh weight basis in GT-, CP- and YM-broth were $42.3\%,\;34.2\%\;and\;9.89\%$, and their turbidity(OD at 660 nm) of the broth without mycelium were 0.14, 0.16 and 0.22, respectively. Total free amino acid content in the mycelium were 928.19 $mg\%$ in YM, 1060.53 $mg\%$ in CP, 764.83 $mg\%$ in GT, and the major free amino acid was lysine in YM, glutamic acid in CP and GT. Total free exo-amino acid contents were 659.75 $mg\%$ in YM, 954.55 $mg\%$ in CP, 838.69 $mg\%$ in GT, and the major in the all broths was glutamic acid Total amino acid derivatives content of the mycelium were in order of CP>GT>YM, and the major was cystathionine in YM, hydroxy proline in CP and GT. The major among exo-amino derivatives was hydroxy proline in the all broth. Exo-AIS content was in order of CP>GT>YM. Acid soluble polysaccharide content of the mycelium was GT($0.69\%$)>YM($0.39\%$)>CP($0.18\%$). The exo-polysaccharide content was in order of GT($0.87\%$)>CP($0.69\%$)>YM($0.09\%$). Alkali soluble polysaccharide content of the mycelium was in order of CP($5.21\%$)>GT($5.18\%$)>YM($4.56\%$), and exo-polysaccharide was in order of GT($6.79\%$)>YM($3.57\%$)>CP($3.01\%$). The alkali soluble polysaccharide eluted from mycelium cultivated in CP broth was supposed to polysaccharide(about 500,000 daltons) composed of hexose and uronic acid bounded with protein(below 10,000 daltons).

• Journal of the Korean Society of Food Culture
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• v.21 no.6
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• pp.644-652
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• 2006

This study was investigated the utilization of pomegranate(P) as coagulants for 새려 manufacture, the quality characteristics and shelf-life on tofu made by P1(fruit juice of pomegranate 1%) and P2(fruit juice of pomegranate 2%) and P3(fruit juice of pomegranate 3%) and P4(fruit juice of pomegranate 4%) and P5(fruit juice of pomegranate 5%) were investigated and compared to G.D.L(C). And also, total microbe and tatal acid of the tofu were determined during storage at $0^{\circ}C^$. The results are summarized as follow P3(493.3g/500ml) compared to C(485g/500ml) showed the highest yield. Turbidity of tofu was increased as the proportion of pomegranate was increased, while pH of tofu was increased as the proportion of pomegranate was decreased. $L^{\ast}^{\ast}^{\ast}$ value of C tofu was highest, $a^{\ast}^{\ast}^{\ast}$ value of tofu was increased as the proportion of pomegranate was increased and $b^{\ast}^{\ast}^{\ast}$ value of C tofu was higher than those of other tofus. The hardness of tofu coagulated with pomegranate showed higher than that of coagulated G.D.L. The cohesiveness of P4, P5 showed higher than C tofu, but those of other tofus showed lower than C tofu. The springiness of tofu showed in the order of P4>P5>P3>P2>P1, gumminess of P5 tofu was highest. The Brittleness of tofu was increased as the proportion of pomegranate increased. The pH value of tofu coagulated with pomegranate increased during storage at $0^{\circ}C^$. During the storage period of tofu, pH and total acid showed a maximum change in C tofu. Generally the total plate counts of bacteria of all tofu increased during storage at $0^{\circ}C^$, and those of tofu with added pomegranate were shown to be lower than C tofu. The results of S.E.M(scanning electron microscopes), the lower hardness showed the more soft and the smaller particle, The particle of C tofu was small and uniformity but the size of P4, P5 tofu showed coarse. In the sensory evaluation of all tofu, sleekness, nutty, chewiness and smoothness, appearance and overall quality was the highest in C tofu. In the sensory evaluation of tofus coagulated with pomegranate. overall quality was the highest in P2.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.11
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• pp.1436-1443
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• 2007

This study was conducted to investigate the shelf-life and quality of Takju added with Glycyrrhiza uralensis (GU), Menthae herba(MH), Schizandra chinensis(SC), and chitosan (C) during storage at $10^{\circ}C$ for 12 days. The viable cell and yeast cell numbers of the Takju treated with Schizandra chinensis powder (SCP) and C were moderately reduced compared with those of the standard. The SC and C Takju were shown to have the lowest oxidations. For turbidity, the SC and C Takju were the most stabilized. Among the treatments, sugar content, pH, and acidity showed no significant differences during storage. However, the lightness, yellowness, and redness value of all the samples were higher than those of the standard. In the sensory evaluation, the standard, SCP, and C scored comparatively higher than the other samples at 0 day. On the other hand, SC and C, GU+MH, and C scored higher after 9 days. From these results, treating Takju with SCP, GU, MH, SC, and C resulted in improvements with regards to its preservation and development of quality.

• Korean Journal of Ecology and Environment
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• v.40 no.2
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• pp.285-293
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• 2007

The study was to determine zonal characteristics of nutrients and chlorophyll and evaluate their trophic relations in Daechung Reservoir. For this study, we compared longterm water quality data among three zones along with trophic state using 1993 to 2002 dataset, obtained from the Ministry of Environment, Korea. Total phosphorous (TP), Secchi depth (SD) and chlorophyll (CHL) showed typical longitudinal declines from the riverine to lacustrine zone, but total nitrogen (TN) was not evident. Largest seasonal variations in TP and CHL occurred during the summer monsoon from July to August. In the reservoir, ambient TN averaged 1.67 mg $L^{-1}$ and ratios of TN : TP averaged 88.04, indicating that nitrogen is not likely limited but phosphorus limitation was evident. Trophic State Index (TSI), based on CHL, TP, and SD, varied depending on the zones and seasons. Mean TSI (TP) in the riverine zone was 62 during the monsoon, indicating a hypertrophic condition, whereas the mean was 40 in the lacustrine, indicating a nearly oligotrophic. Values of TSI (CHL) showed maximum in the transition zone during the monsoon. The deviation analysis of TSI showed that about 65% of TSI (CHL)-TSI (TP) and TSI (CHL)-TSI (SD) values were less than zero and the lowest values were -42, indicating an effect of inorganic turbidity on algal growth in the reservoir. Correlation analysis of CHL vs. SD shewed greater correlation coefficient (p<0.001, r=-0.47) in the transition than other two zones (p<0.001, $r{\leq}-0.40$). Correlation analysis of TP vs. CHL was greatest in the lacustrine and TP was minimum in the lacustrine zone, indicating a lowest yield of algal biomass in the lacustrine. Overall data suggests that zonal response of chlorophyll yield at a given nutrient unit is clearly differed among the longitudinal gradients, so the management strategy such as cross sectional modelling should be provided in each zone.

• Korean Journal of Ecology and Environment
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• v.54 no.1
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• pp.24-38
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• 2021

Microhabitat In the upper stream is created by various environment variables such as the bottom substrate and the physicochemical factors, and may influence the distribution of benthic macroinvertebrates. We investigated the bottom substrate and environmental variables influencing the distribution of benthic macroinvertebrate in 26 stream-type waterways established at upper reaches of Geum River. During study period, total 85 families, 160 species, 9305 individuals of benthic macroinvertebrates were recorded. The stream-type waterways, where the bottom substrates consist mainly of pebble (16~64 mm) and cobble (64~256 mm) or with rapid water velocity (more than 0.2 m/s) and high dissolved oxygen (more than 120%), were supported by high species diversity of benthic macroinvertebrate. Hierological cluster analysis and the nonparametric multidimensional scale (NMDS) divided 26 stream-type waterways into a total of three clusters. In Cluster 1, the invertebrate species, such as Branchiura sowerbyi, Cloeon dipterum, Ischnura asiatica, Paracercion calamorum, and Radix auricularia, closely related to aquatic macrophytes, and Chironomidae spp., Limnodrilus gotoi, and Tanypodinae sp. were abundant in waterways, with high coverage of silt and clay as well as high turbidity and total nitrogen. The benthic macroinvertebrate species (Cheumatopsyche brevilineata, Drunella ishiyamana, Dugesia japonica, Ephemera orientalis, Gumaga KUa, Macrostemum radiatum, Potamanthus formosus, Semisulcospira libertine, Stenelmis vulgaris, and Teloganopsis punctisetae) included in Cluster 2 were dominated in sites with high cover rates of pebble and gravel. Cluster 3 was predominantly covered by the Cobbles, was supported by Simulium sp. Such a clear distinction in the study sites means that each stream-type waterways is governed by a clear habitat environment. In the case of some sites with low species diversity, improvement measures are required to restore nature, such as improving the function of inflows and outflows, creating meandering channel, and inducing the settlement of littoral vegetation.

• Korean Journal of Environmental Biology
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• v.26 no.4
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• pp.392-401
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• 2008

The vertical changes (upper layer: 0$\sim$0.5 m, middle layer: 4$\sim$5 m and lower layer: 8$\sim$10 m) of the limnological variables, zooplankton dynamics and community filtration rate on the phytoplankton were evaluated monthly intervals from November 2004 to June 2007 at the artificiallake (Okjeong Lake). During the study, the annual mean of water temperatures were 15.2$\pm$7.8$^{\circ}C$ (upper layer), 14.5$\pm$7.4$^{\circ}C$ (middle layer) and 13.0$\pm$6.2$^{\circ}C$ (lower layer), respectively (n=30). The vertical variations of most limnological parameters (DO, pH, conductivity and turbidity) were fairly similar, except for chl.-${\alpha}$ concentration. Annual mean of chl.-${\alpha}$ concentration was highest at the upper layer (21.4 ${\mu}g$ L$^{-1}$), while annual means of concentrations were low at the middle layer and lower layer (4.3 ${\mu}g$ L$^{-1}$ and 3.5 ${\mu}g$ L$^{-1}$, respectively). Annual mean of Secchi depth was 261 cm (n=30). A total 45 species of zooplankton were identified (28 rotifers, 12 cladocerans and 5 copepods) during the study. The mean abundance of rotifers was highest (124$\pm$232 ind. L$^{-1}$, n =90) and that of cladocerans and copepods were relatively low (22$\pm$56 ind. L$^{-1}$ and 13$\pm$30 ind. L$^{-1}$) in the whole layer. Total zooplankton abundance increased from late spring to summer, and then declined and remained low throughout the winter. The annual mean of total zooplankton filtration rate for phytoplankton at the lower layer was much higher than that of both layers (upper layer: 44.4$\pm$107.8, middle layer: 95.1$\pm$436.4 and lower layer: 158.2$\pm$436.4 mL L$^{-1}$ day$^{-1}$). Among the major zooplankton community, relative community filtration rate (RCFR, %) of copepods for phytoplankton (50.2$\sim$54.8%) was much higher than that of cladocerans (27.7$\sim$36.3%) and rotifers (8$\sim$17.6%). The seasonal variation of RCFRs of major zooplankton community was observed. The RCFRs of rotifers were high in winter (44.6%, n=9), while the RCFRs of cladocerans were high in summer (58.0%, n=7). In spring and fall, the RCFRs of copepods were high in the whole layer (spring: 67.4%, n=9; fall: 74.4%, n=5).

• Korean Journal of Environmental Biology
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• v.24 no.3
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• pp.230-239
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• 2006

Nutrient over-enrichment as a consequence of anthropogenic loading leads to eutrophication, which has the detrimental effects on river and stream ecosystems. To examine dynamics of factors causing cultural eutrophication in a over-licked mountain stream due to anthropogenic loading, physicochemical parameters were measured from 5 stations in the upper Daecheon stream, Busan, from January 2002 to May 2003. The five study sites were located along the stream gradient. DC1 is upper most clean site, and DC5 is located at the lowest area. Wastewater was released into the stream from just upstream of DC2 site. Water column ammonium and phosphate concentrations were higher during winter than other seasons, while water column nitrate +nitrite concentration did not show clear seasonal variation. Water column ammonium, nitrate+nitrite and phosphate concentrations were lowest at DC1 and highest at DC2 in which waste water loading occurred. TOC and DOC, conductivity, turbidity, and BOD in the water column were also increased drastically at DC2, and then decreased at DC5. Sediment pore water phosphate concentrations during winter and spring were higher than those in summer and fall, while sediment pore water ammonium and nitrate +nitrite concentrations showed no seasonal trend. Sediment pore water ammonium and phosphate concentration were also increased at DC2 and slightly decreased at DC5, while sediment pore water nitrate+nitrite concentration was highest at DC5. Organic matter and inorganic nutrients at up-stream of Daechon stream significantly increased as a result of wastewater discharge, and the nutrient concentrations decreased at low-stream suggesting self-purification ability of the stream.