• Title/Summary/Keyword: and peroxide values

Search Result 517, Processing Time 0.028 seconds

A Study on the Mutagenicity of Autoxidized Soybean Oil (자동산화 대두유의 돌연변이원성에 관환 연구)

  • Lee, Jin-Young;Ahn, Myung-Soo
    • Korean journal of food and cookery science
    • /
    • v.15 no.6
    • /
    • pp.611-617
    • /
    • 1999
  • Commercial soybean oil, which did not contain any antioxidant, were autoxidized at $60{\pm}2^{\circ}C$ for 79 days, and the changes of peroxide value(POV), thiobarbituric acid value(TBAV), conjugated dienoic acid(CDA) content, and fatty acid composition of the oil were studied during the 79 day-storage period. The samples with POV S of 0, 150, 300, 500, 450, 400 and 300 meq./kg oil were used for the test of mutagenic activity. The Ames test was carried out with and without metabolic activation. Bacterial strains used in this study were the histidine auxotrophic strains of S. typhimurium TA100, TA1535, and TA102 for the detection of base pair, and TA98 and TA1537 for frame shift mutations. Each series of samples was disso1ved in tetrahydrofuran(inhibitor free) and tested at doses ranging from 0.05 to 5 mg/plate. The autoxidized soybean oil increased significantly(p<0.05) the number of $His^+$ revertant colonies in cases of TA 98 with S9 mix, TA100 without S9 mix, 1535 and 1537 with and without S9, respectively. The samples having the highest peroxide values showed the strongest mutagenicity. It seemed that the amount of hydroperoxides in the oils was closely related to the mutagenic activity of the respective oils.

  • PDF

Physiological and Functional Properties of Salicornia herbacea (Tungtungmadi) Leaf Extracts

  • Min, Jin-Gi;Son, Kwang-Tae;Kim, Ji-Hoe;Kim, Tae-Jin;Park, Jeong-Heum
    • Preventive Nutrition and Food Science
    • /
    • v.7 no.3
    • /
    • pp.261-264
    • /
    • 2002
  • The physiologically relevant functional properties of various solvent extracts from Salicornia herbacea leaves were investigated by measuring lipid peroxidation, DPPH radical scavenging, nitrite scavenging, and xanthine oxidase inhibition. Ethyl ether, chloroform, ethyl acetate and n-butanol fractions obtained from the 80% aqueous ethanol extracts of Salicornia herbacea leaves showed strong antioxidative activities in linoleic acid methyl esters. Peroxide values (POV) were not significantly different among the samples treated with the different fractions; the incubation time required to reach a peroxide value of 80 meq/kg was about 40 hrs. However, control linoleic acid methyl esters had POV of more than 480 meq/kg after 40 hrs. The DPPH radical scavenging activity of the ethyl acetate fraction was much more effective than diethyl ether, n-butanol, chloroform and water fractions, with an $IC_{50}$/ of 279 $\mu\textrm{g}$/mL, but less effective than ascorbic acid ($IC_{50}$/ : 67 $\mu\textrm{g}$/mL). The nitrite scavenging activities of all fractions increased as pH decreased. Among the fractions, nitrite scavenging activities of diethyl ether and ethyl acetate fractions at pH 1.2 were highest at 59.0 and 56.2%, respectively. The diethyl ether fraction obtained from the 80% aqueous ethanol extract of Salicornia herbacea loaves was the most effective inhibitor of xanthine oxidase of all the solvent extracts at 84% inhibition for a 1 mg/mL concentration. These results suggest that Salicornia herbacea leaf extracts may be effective antioxidants, not only in food stability, but also in human health.

Antioxidative and Nitrite-scavenging Effects of Solvent Extracts from Gyrophora esculenta (석이버섯 용매 추출물의 항산화 및 아질산염 소거작용)

  • 정은재
    • The Korean Journal of Food And Nutrition
    • /
    • v.11 no.4
    • /
    • pp.426-430
    • /
    • 1998
  • This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.

  • PDF

Quantitative Structure-Activity Relationship (QSAR) of Antioxidative Anthocyanidins and Their Glycosides

  • Chang, Hyun-Joo;Choi, Eun-Hye;Chun, Hyang-Sook
    • Food Science and Biotechnology
    • /
    • v.17 no.3
    • /
    • pp.501-507
    • /
    • 2008
  • The quantitative structure-activity relationships (QSAR) study of antioxidative anthocyanidins and their glycosides were evaluated using 4 different assays of Trolox equivalent antioxidant capacity (TEAC), superoxide radical ($O_2^{{\cdot}-}$), hydrogen peroxide ($H_2O_2$), and peroxynitrite radical ($ONOO^-$) scavenging with TSAR software. Four models were developed with significant predictive values ($r^2$ and p value), which indicated that the antioxidant activities were mainly governed by the 3-dimensional structural energy (torsional energy), constitutional properties (the number of hydroxyl and methyl groups), and electrostatic properties (heat of formation, and dipole, quadrupole, and octupole components). This QSAR approach could contribute to a better understanding of structural properties of anthocyanidins and their glycosides that are responsible for their antioxidant activities. It might also be useful in predicting the antioxidant activities of other anthocyanins.

Changes in Quality of Soybean Curd Residue as Affected by Different Drying Methods (건조방법에 따른 비지의 품질변화)

  • 김동수;설명훈;김현대
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.25 no.3
    • /
    • pp.453-459
    • /
    • 1996
  • This study was carried out to determine the changes in quality during the drying process and the optimum drying condition for utilizing soybean curd residue. The quality criteria for soybean curd residue were acid value, peroxide value, fatty acid composition and microbial concentration. The acid values of soybean curd residue were 7.5, 4.5 and 5.9 KOH mg/g upon 12 hour drying with open-air sun, ambient-air blast and warm-air blast, respectively. The numbers of total aerobic bacteria and molds increased remarkably during drying with open-air sunlight, ambient-air blast and warm-air blast except for hot air blast. Among different drying methods, the hot air blast drying(1kg of sample) was the most effective methods, which completed in three hours. Also, the drying method demonstrated a typical drying curve ; settling down, constant rate drying and falling rate drying period were shown within one hour, from one and three hours and after three hours, respectively. Moreover, there was significant variation in the constant drying period for the quality of soybean curd residue.

  • PDF

Antioxidant and α-Amylase Inhibitory Activity of 70% Ethanolic Extract from Morinda citrifolia L. (Noni) (노니가루 70% 에탄올 추출물의 항산화 및 α-Amylase Inhibitory 활성)

  • Lee, Youn Ri
    • The Korean Journal of Food And Nutrition
    • /
    • v.33 no.2
    • /
    • pp.210-214
    • /
    • 2020
  • In this study, polyphenol and flavonoid contents were measured, and DPPH, OH, H2O2 radical scavenging activity, and the α-amylase inhibitory activity were measured to study the antioxidant activity of 70% ethanol extract from Morinda citrifolia L. The polyphenol and flavonoid contents of noni 70% ethanol extract were 29.52 GAE/g and 12.48 CE/g, respectively. Also, the IC50 values of DPPH, hydroxyl radical, and hydrogen peroxide scavenging activity of 70% ethanol extract from noni were 18.70 mg/mL, 26.45 mg/mL, and 35.67 mg/mL, respectively. Measurement of the α-amylase inhibitory activity of 70% ethanol extract from noni showed 45% inhibitory activity at 10 mg/mL.

Effects of Amino Acid Addition on The Lipid Oxidation and Protein Properties in Casein-Fish Oil Model System during Storage (Casein-어유 모델시스템에 있어서 지질산화와 단백질특성변화에 미치는 아미노산 첨가의 영향)

  • 유정희
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.19 no.5
    • /
    • pp.387-394
    • /
    • 1990
  • The effect of lysine or methionine addition on the lipid oxidation and protein properties in casein-fish oil model system during the storage of 21 days at 37$^{\circ}C$ was studied. The peroxide and TBA values were increased markedly and the amino acids addition to the system caused to reduce the extent of lipid oxidation in comparison with that of casein-fish oil system which was a control group. Significant changes in fatty acid composition of each group were observed. Polyenoic acid contents were drastically decreased during the storage in the groups with and without the amino acids additions, . And also significant losses of several amino acids were occurred with the reduction of solubility and digestibility of casein during the storage. However no different effects were observed in both additionsof lysine and methionine to the system.

  • PDF

Oxidative Stability of Green Tea-Added Mayonnaise (녹차를 첨가한 마요네즈의 산화안정성)

  • 박찬성;박어진
    • Korean journal of food and cookery science
    • /
    • v.18 no.4
    • /
    • pp.407-412
    • /
    • 2002
  • The purpose of this study was to investigate the oxidative stability of green tea-added mayonnaise during storage at 5, 15 and 25$\^{C}$. Mayonnaise was prepared with salad oil, egg yolk, sugar, salt and vinegar, and added with 0.1, 0.3 and 0.5% of green tea powder for experiment. Peroxide values (POV) of each mayonnaise were compared during storage for 13 weeks. POVs of control mayonnaise stored at 5, 15 and 25$\^{C}$ for 13 weeks were 28.0, 63.5 and 144.4 meq/kg, respectively. Oxidative stability of green tea-added mayonnaise was increased with increasing concentration of green tea in mayonnaise, but it was decreased with increasing storage temperature. The addition of green tea at 0.5% extended the induction period of mayonnaise significantly (p<0.05) at each temperature. Relative antioxidant effect (RAE) of mayonnaise containing 0.1% of green tea were 226%, 188% and 143% during storage at 5, 15 and 25$\^{C}$, respectively, and it was increased with increasing green tea concentration in mayonnaise. The results suggested that the use of green tea is valuable to inhibit the oxidation of mayonnaise as a natural antioxidant.

The effect of smoking on the quality boiled sausage (훈제 처리가 가열소지에 미치는 영향)

  • 정청송;유상훈
    • Journal of Applied Tourism Food and Beverage Management and Research
    • /
    • v.12 no.2
    • /
    • pp.1-41
    • /
    • 2001
  • The effect of smoking on the quality sausage Sausages were prepared from beef, port, chickens, games, fish and shellfish with polyphosphate salt and spices. Sausage history goes back to 3,000 years of age. The procedure of sausage 1) Meat Curing 2) Meat Control 3) Add Spices 4) Permissible Ingredients 5) Grinding 6) Mixing Emulsion 7) Stuffing 8) Showing 9) Smoking 10) Cooking 11) Drying 12) Packaging Sausage Product to get information on the effect of smoking onto the quality of meat produces. Sausage was smoked under a given conditions. Smoking was conducted as 35f$^{\circ}C$to 6$0^{\circ}C$ the related humidity of smokehouse was still at 60 to 70 percent. Results are as follows: 1. The Penetrated smoke in sausage was prolonged < p.<0.02 when sausage was smoked 2hours at 60~$65^{\circ}C$ the penetrated amount of free acetone in sausage was 0.5mg%. 2. Peroxide value of sausage as heating treatment both at 3$0^{\circ}C$ and at 5$0^{\circ}C$ was prolonged. 3. When sausage was heated at 3$0^{\circ}C$, bacteria of sausage increased In number while at 60~$65^{\circ}C$ bacteria of sausage increased In number fourteen hours. 4. When sausage was treated with smoking the distribution of free amino acids in sausage was changed markedly. 5. The longer smoking time of the products was the higher the content. 6. In case of oak wood smoke flavoring all of test samples. 7. Rapid decrease of does-response mutagenic curve of the smoke flavoring of oak wood and apple wood by in the peak of curve and phenol in the smoke flavoring. Continuous efforts are required to make sausages easily in the butcher shops and in the restaurants. 8. Sausage texture evaluation has 13 rules. It is Elasticity, Surface Moisture, Surface Smoothness, Center Hardness, Skin Toughness, Cohesiveness, Denseness, Moisture Release, Cohesiveness of Mass, Lumpiness, Graininess (of Contents), Skin separation, Oiliness and sensory 11 rules evaluation is color, texture, mold, flavor, sweet test, salty, sourness, bitter, and savory taste. 9. Smoked, component, peroxide value, bacteria, color, free amino acid, tenderness, flavor, shrinkage are important values.

  • PDF

Effect of Rhus chinensis Gall Extract on Liver Function, Plasma Lipid Composition and Antioxidant System in Rats with High Fat Diet (오배자 추출물이 고지방식이를 급여한 흰쥐의 간기능, 혈청지질구성 및 항산화계에 미치는 영향)

  • Choi, Moo-Young;Choi, Eun-Jung;Lee, Eun
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.28 no.3
    • /
    • pp.632-637
    • /
    • 1999
  • The purposes of this study is to investigate the effect of Rhus chinensis gall extract on liver function, plasma lipid composition and antioxidant system in the obese rats with high fat diet for seven weeks. Thirty two male rats of Sprague Dawely strain were divided into four groups. they are normal group (basal diet), control group(high fat diet), III group(high fat diet with Rhus chinensis gall extract 25mg/kg body weight per day) and IV group(high fat diet with Rhus chinensis gall extract 250mg/kg body weight per day). Rats in III group and IV group were administered with Rhus chinensis gall extract accordingly. Weight gains showed a tendency to decrease in rat with Rhus chinensis gall extract group but showed no differences among treatment groups. Food intake and Food efficiency ratio were not significantly different among treatment groups. Plasma total cholesterol showed a tendency to decrease in Rhus chinensis gall extract group. HDL cholesterol showed a tendency to decrease in 25mg/kg of Rhus chinensis gall extract group. However, in the 250mg/kg of Rhus chinensis gall extract group, these values showed no significant difference compared to the control group (p<0.05). GPT activities showed no significant difference among treatment groups. GOT activities showed a tendency to decrease in the groups of Rhus chinensis gall extract groups. Lipid peroxide level was significantly higher in control group than those of normal group. In Rhus chinensis gall extract groups, lipid peroxide level showed a tendency to decrease, but glutathione peroxidase activity was progressively increased. Results of our research in this paper show that Rhus chinensis gall extract might improve liver function, antioxidant system and plasma lipid composition in rats with high fat diet.

  • PDF