Antioxidant and α-Amylase Inhibitory Activity of 70% Ethanolic Extract from Morinda citrifolia L. (Noni)

노니가루 70% 에탄올 추출물의 항산화 및 α-Amylase Inhibitory 활성

  • Lee, Youn Ri (Dept. of Food and Nutrition, Daejeon Health Institude of Technology)
  • 이연리 (대전보건대학교 식품영양과)
  • Received : 2020.03.13
  • Accepted : 2020.04.01
  • Published : 2020.04.30


In this study, polyphenol and flavonoid contents were measured, and DPPH, OH, H2O2 radical scavenging activity, and the α-amylase inhibitory activity were measured to study the antioxidant activity of 70% ethanol extract from Morinda citrifolia L. The polyphenol and flavonoid contents of noni 70% ethanol extract were 29.52 GAE/g and 12.48 CE/g, respectively. Also, the IC50 values of DPPH, hydroxyl radical, and hydrogen peroxide scavenging activity of 70% ethanol extract from noni were 18.70 mg/mL, 26.45 mg/mL, and 35.67 mg/mL, respectively. Measurement of the α-amylase inhibitory activity of 70% ethanol extract from noni showed 45% inhibitory activity at 10 mg/mL.


  1. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200
  2. Dixon AR, McMillen H, Etkin NL. 1999. Ferment this: The transformation of noni, a traditional Polynesian medicine (Morinda citrifolia, Rubiaceae). Econ Bot 53:51-68
  3. Folin O, Denis W. 1915. A colorimetric method for the determination of phenols and phenols derivatives in urine. J Biol Chem 22:305-308
  4. Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16:33-50
  5. Heinicke RM. 1985. The pharmacologically active ingredient of noni. Pac Trop Bot Gard Bull 15:10-14
  6. Hwang HJ, Shin KO, Han KS. 2019. A study on the function and role of Morinda citrifolia L. Korean J Food Nutr 32:275-283
  7. Jeon YH, Kim MH, Kim MR. 2008. Antioxidative and antimutagenic activity of ethanol extracts from Cuscutae semen. Korean J Food Cookery Sci 24:46-51
  8. Kang MH, Choi CS, Kim ZS, Chung HK, Min KS, Park CG, Park HW. 2002. Antioxidative activities of ethanol extract prepared from leaves, seed, branch and aerial part of Crotalaria sessiflora L. Korean J Food Sci Technol 34:1098-1102
  9. Kang MS, Kim SY, Lee YH, Choi JW, Baek OH, Han HK, Kim SN, Kim JB, Park HJ, Cho YS. 2011. Analysis of nutritional components of Euonymus sieboldiana leaves. J East Asian Soc Diet Life 21:918-923
  10. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36:333-338
  11. Kim HY, Lim SH, Park YH, Ham HJ, Lee KJ, Park DS, Kim KH, Kim S. 2011. Screening of ${\alpha}$-amylase, ${\alpha}$-glucosidase and lipase inhibitory activity with Gangwondo wild plants extracts. J Korean Soc Food Sci Nutr 40:308-315
  12. Lee BB, Park SR, Han CS, Han DY, Park E, Park HR, Lee SC. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37:405-409
  13. Lee KW, Je H, Jung TH, Lee YL, Choi JH, Hwang HJ, Shin KO. 2018. Comparison of components and antioxidant activity of cherry, aronia, and maquiberry. Korean J Food Nutr 31:729-736
  14. Lee YH, Kim WK, Jung HA, Oh WK. 2017. Analysis of nutritional components and antioxidant activity of nipa palm (Nypa fruticans Wurmb) flower stalk. Korean J Food Nutr 30:1080-1086
  15. Lee YR. 2016. Analysis of nutritional components and antioxidant activity of roasting Wooung (Burdock, Arctium lappa L.) and Jerusalem artichoke (Helianthus tuberosus L.). Korean J Food Nut 29:870-877
  16. Manian R, Anusuya N, Siddhyraju P, Manian S. 2008. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem 107:1000-1007
  17. Muller HE. 1985. Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl Bakteriol Mikrobiol Hyg Ser A 259:151-154
  18. Pande M, Naiker M, Mills G, Singh N, Voro T. 2005. The Kura files: Qualitative social survey. Pac Health Surveill Response 12:85-93
  19. Singh DR. 2012. Morinda citrifolia L. (noni): A review of the scientific validation for its nutritional and therapeutic properties. J Diabetes Endocrinol 3:77-91
  20. Smirnoff N, Cumbes QJ. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057-1060
  21. Sunder J, Jeyakumar S, Sujatha T, Kundu A. 2013. Effect of feeding of morical: A herbal based supplement on production and egg quality in Japanese quail. Adv Anim Vet Sci 1:157-160
  22. Tabrah FL, Eveleth BM. 1966. Evaluation of the effectiveness of ancient Hawaiian medicine. Hawaii Med J 25:223-230
  23. Wang MY, Su C. 2001. Cancer preventive effect of Morinda citrifolia (noni). Ann NY Acad Sci 952:161-168
  24. Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, Anderson G. 2002. Morinda citrifolia (noni): A literature review and recent advances in noni research. Acta Pharmacol Sin 23:1127-1141
  25. Xu ML, Wang L, Xu GF, Wang MH. 2011. Antidiabetes and angiotensin converting enzyme inhibitory activity of Sonchus asper (L.) hill extract. Korean J Pharmacogn 42:61-67
  26. Yu MH, Im HG, Lee HJ, Ji YJ, Lee IS. 2006. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zyzyphus jujuba var. inermis Rehder. Korean J Food Sci Technol 38:128-134