• 한국동물위생학회지
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• 제41권1호
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• pp.29-39
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• 2018

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

• 대한공간정보학회지
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• 제20권2호
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• pp.101-107
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• 2012

본 연구는 우리나라의 구재역 문제가 심각한 사회문제로 대두되면서 현재의 방역대 설정에 대한 문제점을 분석하여 사전 조치를 통한 피해를 최소화하기 위하여 수행하였다. 이를 위하여 구제역 발생 현황을 분석하고, 전국 국도의 도로망도와 행정구역 경계 등의 자료를 이용하여 도로를 통해 차량으로 전염되는 것을 가정한 후 네트워크 분석을 수행하였다. 이와 같이 분석된 결과를 이용하여 전파시간을 산정하고, 그 결과를 도로 직선거리 및 도로 실거리로 구분하여 직선 이동속도 및 도로 실거리 이동속도를 국도별 및 행정구역별로 산출하여 현재의 구제역 방역대 설정방법에 대한 문제점을 분석하였다. 따라서, 본 연구는 구제역 발생지역을 중심으로 전파속도를 산출한 결과 평균 직선 전파속도는 53.9km/day, 평균 도로 실거리 전파속도는 71.1km/day로 분석되어 기존 방역대 설정에 문제가 있었음을 알 수 있었다.

• 한국산학기술학회논문지
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• 제12권11호
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• pp.5063-5070
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• 2011

구제역은 소 돼지, 양, 염소 및 사슴 등의 발굽이 두 개인 가축 또는 야생 동물에서 발생하는 질병이다. 본 연구는 구제역에 대한 지식, 인식과 생활 습관을 경기도 총 5개 지역 초등학생 398명을 대상으로 조사하였다. 구제역이 무엇인지 들어보았는지 조사한 결과 구제역 교육을 받은 그룹은 49명 92.5%가 '있다'고 하였으며, 구제역 교육 받지 않은 그룹은 139명 68.5%가 '있다'고 하였다. '구제역 바이러스는 섭씨 $50^{\circ}C$ 이상에서 파괴되기 때문에 조리시 모두 바이러스 균이 모두 죽는다'고 지식을 조사한 결과 구제역 교육 받은 그룹은 26명 49.1%, 구제역 교육을 받지 않은 그룹은 82명 40.4%만 올바르게 알고 있는 것으로 나타났다. 구제역과 같은 질병에 대한 교육은 언제하는 것이 좋다고 생각하는지 조사한 결과 두 그룹 모두 초등학교 시기라고 응답하였다. 구제역은 다국가간에 수차례 감염 발생이 일어나고 있는 질병으로 우리나라도 안전하지 못한 상재지역이다. 이에 국민들이 올바른 인식과 지식으로 대비해야 할 것이다.

• 한국산학기술학회논문지
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• 제17권9호
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• pp.292-301
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• 2016

구제역 발생에 따른 피해는 매우 크기 때문에 구제역의 피해를 최소화하기 위해서는 선제적인 구제역 진단 및 대응이 필수적이다. 주요 구제역 증상은 소의 체온 상승, 식욕 부진, 유량 감소, 입 발굽 유방에 물집 형성 등이며, 이중 확인하기 가장 쉽고 빠른 방법은 체온을 검사하는 방법이다. 본 논문에서는 선제적 구제역 대응을 위해 소 발굽 검출 알고리즘을 개발 구현하고, 축사에 고해상도 카메라 모듈과 열화상 카메라, 온습도 모듈 설치하여 발굽 검색 테스트를 시행하였다. 본 연구에서 개발한 알고리즘과 시스템을 통해 구제역 의심 가축의 조기 상황 대처를 할 수 있으며, 가축의 최적 성정 환경을 조성할 수 있다. 특히, 본 연구에서는 기존의 휴대용이 아닌 열화상 카메라를 활용한 구제역 대응 시스템은 축사에 고정으로 부착하여 별도 인력을 필요로 하지 않고 이미지 알고리즘을 통하여 가축의 발굽 온도를 자동 측정하는 기능과 스마트 폰을 활용한 자동 경고 기능을 가지고 있다. 이러한 시스템은 실시간을 구제역 가능성 예측을 가능케 하며, 별도의 인력이 없이 가축 질병에 대한 초동 방역 대응을 할 수 있다.

• 한국동물위생학회지
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• 제34권2호
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• pp.95-102
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• 2011

Foot-and-mouth disease (FMD) is one of the most infectious diseases affecting cloven-hoofed animals including cattle, sheep, goats, and pigs. Seven serotypes of foot-and-mouth disease virus with multiple subtypes within each serotype have been identified until now. In particular, it has been demonstrated that the outbreak of the serotype Asia1 reported from China, Mongolia and North Korea since 2005 is mostly classified into genetic group V. Though it has been recommended that Asia1 Shamir strain can be used as a high priority vaccine by World References Laboratory for FMD, the complete nucleotide sequences of the strain has not yet been determined. In this study, to be prepared for Asia1 type viruses that may be brought into Korea, the complete genome sequence of this vaccine strain Asia1 Shamir including its 5' and 3' non-coding region was identified.

• 한국멀티미디어학회논문지
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• 제20권3호
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• pp.474-481
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• 2017

In Korea, foot-and-mouth disease has not been reported for several decades, but it began to develop again in 2000. For 2010~2011, when the worst occurred, 3.5 million animals were disposed of resulting in a loss of 2.8 trillion won. In order to prevent the harmful effects of foot-and-mouth disease, vaccination and housing management are being implemented. Despite these measures, foot-and-mouth disease is infected with air through the respiratory tract and accompanies fever after latency. Therefore, it is recognized that measuring and managing the body temperature of livestock at the early stage is the first step of managing this disease. In this paper, we propose a temperature monitoring system that can measure the body temperature by incorporating temperature sensor mounted in ear-tag of cattle and collect body temperature data of each individual cattle through BLE into the control server. The proposed body temperature monitoring system has various advantages such as easy installation without the help of livestock specialists and not damaging the organs of the livestock. So, it is possible to manage the abnormal symptom of cattle in real time and it is believed that the proposed monitoring system will revolutionize the prevention of foot-and-mouth disease.

• 대한간호학회지
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• 제40권5호
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• pp.747-755
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• 2010

Purpose: This study was conducted to investigate the degree of dry mouth and oral health-related quality of life and to identify factors contributing to oral health-related quality of life for community-dwelling elders. Methods: A descriptive correlational study design was used. Participants were 156 older adults from two senior welfare centers. Data were collected on February 21, 22 and 29, 30, 2009 using structured questionnaires. Enter type multiple regression analysis was used to identify factors influencing oral health-related quality of life according to general and oral health characteristics. Results: There were significant differences in oral health-related quality of life according to living arrangement, insurance, smoking, number of natural teeth, and denture type. The oral health-related quality of life had significant correlations with the number of chronic disease, number of medications, and dry mouth. Factors influencing oral health-related quality of life for community-dwelling older adults were dry mouth, number of chronic disease, and medical aid, which explained about 47.9% of total variance. Conclusion: These results indicate that in order to promote oral health-related quality of life for older adults, prevention or management of chronic diseases as well as oral health and dry mouth are needed for this population, and especially economically poor elders.

• 한국시스템다이내믹스연구
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• 제14권4호
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• pp.37-62
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• 2013

The purpose of this paper is to find out some clues about effectiveness and implementing timing of the control measures against Foot-and-Mouth Disease (FMD) in Korea. To do that, the case of FMD, which broke out during November 2010 in Korea, is examined and constructed as a system dynamics simulation model. The implications of simulation result are as follows. First of all, it is most effective measure to prevent the movement of vehicle from infected farmhouse to other noninfected farmhouses, which are located far away, in the early stage of FMD diffusion. Secondly, earlier vaccination can be adopted as a reliable means to control the epidemic when FMD spreads widely. Finally, reducing the time to vaccinate the whole noninfected livestock is a little help to restrain the additional infections of FMD.

• 한국임상수의학회지
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• 제21권2호
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• pp.172-176
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• 2004

We described the clinical signs, gross lesions, histopathological lesions, and electronmicroscopy of pig Foot-and-Mouth Disease cases which had occurred in 2002, Korea. Grossly affected pigs showed vesicles on snout, tongue, coronary band, and udder. Histoapthologically, severe intercellular edema and necrosis of prickle cells, and separation between epidermis and dermis were found on the mucosa of tongue and coronary band. And myocardial necrosis and mononuclear cells infiltration in myocardium were found. Electronmicroscopically numerous picornavirus particles(18∼22 nm in diameter) were found in the cytoplasm of prickle cells. By those results, we confirm that those cases were typical FMD cases.

• 한국자기공명학회논문지
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• 제7권2호
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• pp.89-97
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• 2003

Foot-and-mouth disease virus (FMDV) belongs to the aphthovirus genus within the picornavirus which has a single copy of a positive sense RNA. The translation initiation process of FMDV occurs by a cap-independent mechanism directed by a highly structured element (∼435 nt) termed an internal ribosome entry site (IRES). We have designed and prepared FMDV 4-2 RNA (28nt) by in vitro transcription. The 2D NMR data revealed that FMDV 4-2 IRES domain RNA has a flexible loop and bulge conformation. In further study, we need to make an isotope labeled RNA sample and conduct 3D NMR experiments to completely determine the 3D structure. This study may establish a new drug design strategy to treat foot-and mouth disease.