• Korean Journal of Agricultural Science
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• v.6 no.1
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• pp.21-26
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• 1979

To compare the effect of dikegulac (sodium 2,3 : 4, 6-di-O-isopropylidene-2-keto-L-gulonate) and ancymidol (${\alpha}$-cyclopropyl-${\alpha}$-(4-methoxyphenyl)-5-pyrimidinemethanol) on the growth and flowering responses of Salvia splendens cv. Bonfire, foliar spray of dikegulac at rates of 0.06 and 0.12% and ancymidol at rates of 50 and 100 ppm, and soil drench of ancymidol at rates of 0.1 and 0.5 mg/pot were treated. Dikegulac reduced the plant height and induced the lateral shoot development by disrupting apical dominance, and thus increased the number of inflorescences and retarded flowering date. Dikegulac treatment greatly increased chlorophyll content of leaf, especially chlorophyll a. Ancymidol reduced the plant height without disrupting apical dominance and retarding flower ing date, but did not increase inflorescence number except soil drench at rate of 0.1 mg/pct. Ancymidol treatment significantly increased chlorophyll content of leaf, especially chlorophyll a. Dikegulac can be effectively used as a pinching agent and branching agent for salvia.

• Korean Journal of Weed Science
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• v.17 no.2
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• pp.207-213
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• 1997

This study was conducted to correlate changes in plant growth and flowering behavior with inhibition of gibberellin synthesis following application of GA biosynthesis inhibitor. Two sorghum genotypes, wild-type and phyB-1(phytochrome B mutant) which grow fast and flowers early relative to the wild-type, were used. Both growth and floral initiation of these two genotypes were greatly affected by ancymidol concentration increased. However, these growth inhibition and delayed flowering are almost completely overcome by simultaneous applications of 31.6ppm $GA_3$. The ability of $GA_3$ to reverse the effect of the inhibition on both growth and floral initiation in sorghum suggests a role for native GAs in sorghum flowering. This result was contrast to the fact that in some long day plants GA biosynthesis inhibitors will inhibit shoot elongation but not floral initiation. In sorghum, inhibition of vegetative growth by GA biosynthesis inhibitor is accompanied by a delay in flowering. Ten ppm of ancymidol treatments drastically reduced all early-13-hydroxylation pathway GAs($GA_{12}$, $GA_{53}$, $GA_{19}$, $GA_{20}$, $GA_3$, and $GA_8$) levels.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.165-170
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• 2010

This study was carried out to investigate the effect of three different growth retardants for applying pot or bedding plant with 'Rialto', 'Elodie', and 'Raizan'. Ancymidol, uniconazole, and paclobutrazol were applied as a drench at various concentrations. In Oriental hybrid lily 'Rialto', no changes were observed not only plant height but also leaves and flowers in paclobutrazol treatment. But the growth of plant treated with ancymidol was inhibited above 30% compare to control regardless of concentrations. However, numbers of leaf and flower per plant were similar to those of controls. Plant height was similar to nontreated control in $1mg{\cdot}L^{-1}$ uniconazole treatment but that reduced about 30% in drench application of 5 and $10mg{\cdot}L^{-1}$ uniconazole. Asiatic hybrid lily 'Elodie' was not different in growth between application of paclobutrazol and nontreatment as 'Rialto'. While, in all ancymidol treatments, plant height was 50~60% shorter than that of control. Also, numbers of leaves were reduced and days to flowering were slightly delayed in $30mg{\cdot}L^{-1}$ ancymidol treatment as compared with the control. All traits except plant height of $1mg{\cdot}L^{-1}$ uniconazole were similar to control but there were differences of statistical significance between 5, $10mg{\cdot}L^{-1}$ uniconazole drench and nontreated control. Lilium ${\times}$ formolongi 'Raizan' like above two cultivars had no dwarf effect on height in paclobutrazol treatment. In drench application of ancymidol, plant height reduced over 60% than that of control regardless of concentrations. Especially, days to flowering were a little delayed with $30mg{\cdot}L^{-1}$ paclobutrazol treatment. Plant height of $10mg{\cdot}L^{-1}$ uniconazole was about 26% shorter than that of control, but other traits were no differences between uniconazole treatment and control. In result, the drench application of ancymidol and uniconazole was pretty effective for usage as pot lily without great changes of quantitative traits.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.38-43
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• 2010

To develop Korean native lilies as mini-potted plants, the effects of plant growth retardants on inhibiting stem elongation and improving flowering rate were evaluated. Bulblets of Lilium concolor var. parthneion (5-7 g) and L. dauricum (3-4 g fresh wt) derived from in vitro culture and then enlarged by field culture were applied with 0, 25, 50, or $100mg{\cdot}L^{-1}$ ancymidol, diniconazole, and uniconazole through bulb dipping, foliar spray, and drench. In L. concolor var. parthneion, uniconazole dipping most effectively reduced plant height by 60.7-78.3% depending on concentration compared with control without decreasing flowering percentage. In L. dauricum, dipping into diniconazole solution significantly retarded the plant height by 70.0-86.3% and improved flowering percentage by 8.3-18.2% compared to control of 0%. Drench of $25-50mg{\cdot}L^{-1}$ ancymidol was also effective on inhibition of stem elongation and improvement of flowering rate. Therefore, dipping into $50-100mg{\cdot}L^{-1}$ uniconazole solution in L. concolor var. parthneion and dipping into $25mg{\cdot}L^{-1}$ diniconazole solutions or drench of $25mg{\cdot}L^{-1}$ ancymidol in L. dauricum can be useful to produce the mini-potted plants.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.139-144
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on endogenous ABA-like substance content and GA-like substance activity in seedlings of Sedirea japonica cultured in vitro. When seedlings of Sedirea japonica were treated with low concentration of 0.05 mg/L Uniconazole, 0.1 mg/L Ancymidol and 0.3 mg/L Paclobutrazol, the content of ABA-like substances of the leaf was lower than that of the control. However, the activity of GA-like substances was similar or higher in treated seedlings. In the mid and high concentrations of three kinds of growth retardants, the ABA-like substance content was increased, but GA-like substance activity was inhibited. The content of ABA-like substances in the root was lower in 0.05 and 0.2 mg/L Uniconazole, 0.2 mg/L Ancymidol and 0.1 mg/L Paclobutrazol treatments than that of the control, but in the mid and high concentration treatments, the content was increased. GA-like substance activity in low concentration was increased but in the mid and high concentration, the activity was inhibited compared with low concentration treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.300-304
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• 2004

Gibberellic acid did not affect ethylene production, whereas gibberellin biosynthesis inhibitors triggered ethylene production in dormant tubers. Gibberellic acid did not induce sprouting of dormant tubers, however, treatment of gibberellin biosynthesis retardants enhanced sprouting rates. Sprouting rate in ancymidol-treated tubers was highest among gibberellin biosynthesis retardants. Sprouting rate of tubers treated with ancymidol increased to $91.4\%$. Batatasin-III content in $GA_3$ treated tuber was increased in the highest concentration $(30{\mu}g\;I^{-1})$. Tubers treated with mepiquat chloride, Batatasin-I was increased steadily, but contents of Batatasin-III and V showed dramatic decrease at the $ 1,000{\mu}g\;I^{-1})$ concentration. This infers that gibberellin biosynthesis retardants play key roles in promoting breaking dormancy on dormant tubers of Chinese yam.

• Korean Journal of Plant Tissue Culture
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• v.25 no.3
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• pp.141-146
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• 1998

Feeding experiment of [$^3$H] 5-epi-aristolochene (5-EAS) demonstrated in suspension cultures of tobacco (Nicotiana tabacum L.) that 5-EAS hydroxylase activity was absent from control cells, but induced to a maximum level within 18 h after the addition of cellulase, and was very similar to induction pattern of sesquiterpene cyclase. This result suggest that the conversion of 5-EAS to capsidiol is catalyzed by at least one elicitor-inducible hydroxylase. Cytochrome P450 inhibitors, ancymidol and ketoconazole, suppressed the elicitor-induced capsidiol accumulation by inhibiting hydroxylase activity, suggesting that the hydroxylase may be a Cyt P450 type enzyme.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.499-504
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.

• Journal of Life Science
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• v.9 no.4
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• pp.408-413
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• 1999

Extracellular capsidiol, sesquiterpenoid phytoalexin, in the medium of pepper (Capsicum annuum L.) suspension cells was not identified from control cells, but highly accumulated in the elicitor-induced cells within 6 hours after the addition of 0.05$\mu\textrm{g}$/$m\ell$ cellulase. Capsidiol production in elicitor-induced cells was markedly suppressed by cytochrome P450 inhibitors, such as ancymidol and ketoconazole demonstrating that biosynthesis of capsidiol is catalyzed by at least on hydroxylation enzyme in the biochemical pathway. Based on protein electrophoresis, two bands, 23.0kDa and 27.5kDa, were identified as newly synthesized polypeptides in the elicitor-induced suspension cells, suggesting that pepper cells which were subjected to elicitor treatment activate specific gene(s) for capsidiol biosynthesis in cultured cells.

• Korean Journal of Plant Tissue Culture
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• v.21 no.4
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• pp.239-246
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• 1994

The genotypic (2n, 3n, 4n) response of watermelon in vitro shoot tip culture was evaluated. Different genotypes had similar response in terms of shoot formation and growth. Shoot formation was better at lower concentration of 0.3 mg/L BA and higher concentration of 5-10.0 mg/L 2iP and kinetin, but growth of newly formed shoot was inhibited. With further subculture, kinetin did not promote shoot formation Better shoot formation was observed at 0.3-0.5 mg/L BA. Combination of 0.3 mg/L BA and 0.3-0.5 mg/L BA was effective in shoot multiplication, growth and induction of more internodes. Varrying levels of light intensity and agar concentration did not affect the performance of tetraploid plants. Higher light intensity and agar concentrations decreased the number of shoot formed in triploid plane. Growth in both genotype, however was inhibited. Higher light intensity was found to promote leaf senescence in all genotypes. All growth inhibitors decreased the number of shoots formed and slowed plant growth there by prolonging duration of cultures. Growth inhibitors were to observed to decrease incidence of hyperhydricity in culture. No difference in shoot formation was observed in each of the concentrations used in Ancymidol, TIBA, CCC and PP333. Shoot formation and growth was more inhibited in ABA treatments. Leaf expansion and growth was poor in all treatments.