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Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Magnoliae Flos by HPLC

  • Fang, Zhe;Shen, Chang Min;Moon, Dong-Cheul;Son, Kun-Ho;Son, Jong-Keun;Woo, Mi-Hee
    • Bulletin of the Korean Chemical Society
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    • v.31 no.11
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    • pp.3371-3381
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    • 2010
  • In this study, quantitative and pattern recognition analysis for the quality evaluation of Magnoliae Flos using HPLC/UV was developed. For quantitative analysis, eleven major bioactive lignan compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6\;mm$, $5\;{\mu}m$) with isocratic elution of acetonitrile and water with 1% acetic acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 278 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of eleven major compounds in the extract of Magnoliae Flos. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty one reference samples corresponding to seven different species of Magnoliae Flos and nine samples purchased from market. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Magnoliae Flos.

Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Cimicifugae Rhizoma by HPLC

  • Fang, Zhe;Moon, Dong-Cheul;Son, Kun-Ho;Son, Jong-Keun;Min, Byung-Sun;Woo, Mi-Hee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.1
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    • pp.239-246
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    • 2011
  • In this study, quantitative and pattern recognition analysis for the quality evaluation of Cimicifugae Rhizoma using HPLC/UV was developed. For quantitative analysis, three major bioactive phenolic compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}M$) with isocratic elution of acetonitrile and water with 0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 323 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cimicifugae Rhizoma. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twelve reference samples corresponding to five different species of Cimicifugae Rhizoma and seventeen samples purchased from markets. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Cimicifugae Rhizoma.

Analysis of changes in dose distribution due to respiration during IMRT

  • Shin, Jung-Suk;Shin, Eun-Hyuk;Han, Young-Yih;Ju, Sang-Gyu;Kim, Jin-Sung;Ahn, Sung-Hwan;Kim, Tae-Gyu;Jeong, Bae-Kwon;Park, Hee-Chul;Ahn, Young-Chan;Choi, Doo-Ho
    • Radiation Oncology Journal
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    • v.29 no.3
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    • pp.206-213
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    • 2011
  • Purpose: Intensity modulated radiation therapy (IMRT) is a high precision therapy technique that can achieve a conformal dose distribution on a given target. However, organ motion induced by respiration can result in significant dosimetric error. Therefore, this study explores the dosimetric error that result from various patterns of respiration. Materials and Methods: Experiments were designed to deliver a treatment plan made for a real patient to an in-house developed motion phantom. The motion pattern; the amplitude and period as well as inhale-exhale period, could be controlled by in-house developed software. Dose distribution was measured using EDR2 film and analysis was performed by RIT113 software. Three respiratory patterns were generated for the purpose of this study; first the 'even inhale-exhale pattern', second the slightly long exhale pattern (0.35 seconds longer than inhale period) named 'general signal pattern', and third a 'long exhale pattern' (0.7 seconds longer than inhale period). One dimensional dose profile comparisons and gamma index analysis on 2 dimensions were performed. Results: In one-dimensional dose profile comparisons, 5% in the target and 30% dose difference at the boundary were observed in the long exhale pattern. The center of high dose region in the profile was shifted 1 mm to inhale (caudal) direction for the 'even inhale-exhale pattern', 2 mm and 5 mm shifts to exhale (cranial) direction were observed for 'slightly long exhale pattern' and 'long exhale pattern', respectively. The areas of gamma index >1 were 11.88 %, 15.11%, and 24.33% for 'even inhale-exhale pattern', 'general pattern', and 'long exhale pattern', respectively. The long exhale pattern showed largest errors. Conclusion: To reduce the dosimetric error due to respiratory motions, controlling patient's breathing to be closer to even inhaleexhale period is helpful with minimizing the motion amplitude.

A Study on the Vibration Characteristics Analysis of Composite Materials by Using Electronic Speckle PatternInterferometry Method (전자처리 스페클 패턴 간섭법을 이용한 복합재료의 진동 특성 해석에 관한 연구)

  • 김형택;정현철;양승필
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1995.10a
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    • pp.388-392
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    • 1995
  • The Electronic Speckle Pattern Interferometry(ESPI) has been applied to many technical problems such as deformation and displacement measurement, strain visualization and surface roughness monitoring. Composite materials have various complicated characteristics depending on the ply materials,ply orientations,ply stacking sequences and boundary conditions. Therefore, it is difficult to analyze composite material. For efficient use of composit materials in engineering applications, the dynamic behavior such as, natural frequencies and modal patterns should be identified. This studying presents FEM results for the free vibration of symmetrically laminated composite as [30/-30/90] $_{s}$. The natural frequencies of laminated composite rectangular plates having the boundary condition(:2-edge clamped) are experimentally obtained. In order to demonstrate the validity of the experiment,FEM analysis using ANSYS was performed and natural frequencies experimentally obtained is compared with calculated by FEM analysis. The results obtained from both experiment and FEM analysis show a good agreement.t.

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Seismic evaluation of RC stepped building frames using improved pushover analysis

  • Sarkar, Pradip;Prasad, A. Meher;Menon, Devdas
    • Earthquakes and Structures
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    • v.10 no.4
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    • pp.913-938
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    • 2016
  • 'Stepped building' frames, with vertical geometric irregularity, are now increasingly encountered in modern urban constructions. This paper proposes a new approach to determine the lateral load pattern, considering the contributions from the higher modes, suitable for pushover analysis of stepped buildings. Also, a modification to the displacement coefficient method of ASCE/SEI 41-13 is proposed, based on nonlinear time history analysis of 78 stepped frames. When the newly proposed load pattern is combined with the modified displacement coefficient method, the target displacement for the stepped building frame is found to match consistently the displacement demand given by the time history analysis.

Case Study of Blasting Pattern Design for Tunnelling in Which Considered Blast Induced Vibration Affected Across Buildings (터널 굴착 시 주변 구조물에 미치는 영향을 고려한 발파 설계 사례)

  • Baek, Seung-Kyu;Choo, Seok-Yeon;Yoon, Jong-O;Baek, Un-Il;Park, Hyung-Seop
    • Tunnel and Underground Space
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    • v.16 no.5 s.64
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    • pp.377-386
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    • 2006
  • In generally blasting pattern design is carried out in-situ borehole blasting test and its analysis. We added the 3D numerical analysis for blast induced vibrations. This paper is case study of 3D numerical analysis in which considered blast induced vibration affected across buildings, and then we design the blasting pattern of tunnel excavation.

Analysis of the Ease in Basic Bodice Pattern Using 3-D Measuring Instrument (3차원 계측장치를 이용한 길 원형의 여유량 분석)

  • Shim, Kue-Nam;Suh, Jung-Kwon;Lee, Won-Ja
    • Fashion & Textile Research Journal
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    • v.2 no.3
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    • pp.239-245
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    • 2000
  • The purpose of this study was for analysis of ease about basic bodice pattern, as the first step of the research process for the drawing method of basic bodice for women in their twenties. The five selected basic bodice were made and they were worn by FRP body The garment space of each bodice was measured by analysis of the garment space of each section in figure of polymerization of cross section by a 3-D measuring instrument. The research suggests that this compared analysis is an objective reference. This analysis not only of the area of cross section of garment space and ease but also of the girth of the body shape and wearing shape, using the PAD system and 3-D measuring instrument, can be helpful in making garment patterns.

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Spatial Pattern of Larix gmelini in a Spruce-fir Valley Forest of Xiaoxing'an Mountains, China

  • Jin, Guangze;Liu, Liang;Liu, Zhili;Kim, Ji-Hong
    • Journal of Korean Society of Forest Science
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    • v.99 no.5
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    • pp.720-725
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    • 2010
  • On the basis of vegetation data in the 9.12 ha (380 m ${\times}$ 240 m) permanent sample plot of the spruce-fir valley forest in Liangshui National Reserve of Xiaoxing'an Mountains, the study was conducted to evaluate spatial distribution pattern and spatial association by using point pattern analysis for living and dead trees of Larix gmelini by DBH size class. The number of L. gmelini were counted as 59 living stems/ha (6.42 $m^2$/ha of basal area) and 34 dead stems/ha (2.86 $m^2$/ha of basal area). The distributional curve of diameter class exhibited bimodal shape. The analysis of spatial distribution patterns of all living larch stems noted the clumped distribution on the whole. The size of larch aggregates of dead stems was decreased as diameter class was increased. The distribution of dead stems became gradually randomized with decreased clumped size as the scale increased. Living stems and dead stems of the larch had positive spatial association at most of scales, illustrating that the occurrence of mortality of the larch tree was closely related to the distribution pattern of living larch trees.

Classficiation of Bupleuri Radix according to Geographical Origins using Near Infrared Spectroscopy (NIRS) Combined with Supervised Pattern Recognition

  • Lee, Dong Young;Kang, Kyo Bin;Kim, Jina;Kim, Hyo Jin;Sung, Sang Hyun
    • Natural Product Sciences
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    • v.24 no.3
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    • pp.164-170
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    • 2018
  • Rapid geographical classification of Bupleuri Radix is important in quality control. In this study, near infrared spectroscopy (NIRS) combined with supervised pattern recognition was attempted to classify Bupleuri Radix according to geographical origins. Three supervised pattern recognitions methods, partial least square discriminant analysis (PLS-DA), quadratic discriminant analysis (QDA) and radial basis function support vector machine (RBF-SVM), were performed to establish the classification models. The QDA and RBF-SVM models were performed based on principal component analysis (PCA). The number of principal components (PCs) was optimized by cross-validation in the model. The results showed that the performance of the QDA model is the optimum among the three models. The optimized QDA model was obtained when 7 PCs were used; the classification rates of the QDA model in the training and test sets are 97.8% and 95.2% respectively. The overall results showed that NIRS combined with supervised pattern recognition could be applied to classify Bupleuri Radix according to geographical origin.

Characteristic Classification of Aroma Oil with Gas Sensors Array and Pattern Recognition (가스센서 어레이와 패턴인식을 활용한 아로마 오일의 특성 분류)

  • Choi, Il-Hwan;Hong, Sung-Joo;Kim, Sun-Tae
    • Journal of Sensor Science and Technology
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    • v.27 no.2
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    • pp.118-125
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    • 2018
  • An evaluation system for an electronic-nose concept using three types of metal oxide gas sensors that react similarly to the human olfactory cells was constructed for the quantitative and qualitative evaluation of aroma fragrances. Four types of aroma fragrances (lavender, orange, jasmine, and Roman chamomile), which are commonly used in aromatherapy, were evaluated. All the gas sensors reacted remarkably to the aroma fragrances and the good correlation of r=0.58-0.88 with the aromatic odor intensities by olfaction was confirmed. From the results of the analysis of an electronic-nose concept for classifying the characteristics of aroma oil fragrances, aroma oils could be classified using the fragrance characteristics and oil extraction methods with the cumulative variability contribution rate of 95.65% (F1: 69.65%, F2: 26.03%) by principal component analysis. In the pattern recognition based on the artificial neural network, the four aroma fragrances were 100% recognized through the training data of 56 cases (70%) out of 80 cases, and the pattern recognition rate was 57.1%-71.4% through the validation and testing data of 24 cases (30%). The pattern recognition success rate through all confusion matrices was 82.1%, indicating that the classification of aroma oil fragrances using the three types of gas sensors was successful.