Xylose (glucose) isomerase was purified to homogeneity from cell-extracts of Streptomyces chibaensis J-59 via ammonium sulfate precipitation followed by chromatography on DEAE-cellulose, and gel filtration on Sephacryl S-300. The purified enzyme is a homotetramer with a native molecular mass of 180 kDa and a subunit molecular mass of 44 kDa. The amino acid N-terminal sequence of glucose isomerase from S. chibaensis J-59 was determined to be Ser-Tyr-Gln-Pro-Thr-Pro-Glu-Asp-Arg-Phe-Thr-Phe-Gly-Leu. The first 14 amino acids of the N-terminal sequence of the enzyme showed strong analogies with N-terminal sequences of glucose isomerase produced by other Streptomyces spp. The optimum pH and temperature for activity were 7.5 and 85, respectively. The purified enzyme required $Mg^{2+}$, $Co^{2+}$, and $Mn^{2+}$ for the activity, $Mg^{2+}$ being the most effective. The enzyme was not inhibited by $Ca^{2+}$, but was inhibited by $Hg^{2+}$, $Ag^+$, and $Cu^{2+}$. The $K_m$, $V_{max}$, and $k_{cat}$ values of S. chibaensis J-59 isomerase for glucose were 83 mM, 40.9 U/mg, and $1,843min^{-1}$, respectively. In the presence of $Co^{2+}$, cell-free enzymes retained 100% without loss of activities by the heat-treatment at $70^{\circ}C$ for 7 days. The enzyme retained 50% residual activity after heating at $85^{\circ}C$ for 13.5 h, at $90^{\circ}C$ for 126 min. The enzyme is more thermostable than any other glucose isomerases of Streptomyces spp.
The research has the aim of defining the special characteristics of the functionalist fashion design in the 1900s by understanding the essence of functionalism in the sociocultural contexts. Giving careful consideration to functionalist fashion design in the 1990s makes it possible to foresee the way of fashion design to meet the needs of the modems and men of the future world. To obtain the purposes, documentary study and demonstrative study with fashion photographs have been executed. Main results are as follows. Functionalism in the 1990s has been developed in different ways from the analogies in the beginning of the 20th century. It doesn't belong to an analogy but has the fused characteristics. It can be classified into techno functionalism influenced by new technology Pure functionalism related to minimalism. casual functionalism for those who are crazy about the speed and sports and enjoy the freedom and comfort and symbolic functionalism. Mechanical analogy has been developed into techno functionalism. Pure functionalism has come from the blend of mechanical and moral analogy, casual functionalism from mechanical. organic and moral analogy. and symbolic functionalism from organic and moral analogy. Since fashion design has much closer relation to human body and movements. it has been expressed considering a division of men and women and the places and times for the designs more than other designs. More casual manners of formal wear are distinct elements in functionalist fashion design in the 1990s. The 20th century general cultural phenomena including post modernism can be explained by eclectic functionalism. Functionalism considering Practical and conceptional functions ill continue and techno functionalism and casual functionalism in future fashion design will be expressed more strongly with new technology and casual trends of life style.
This study analyzes on the reasoning in the process of justification and mathematical problem solving in our primary mathematics textbooks. In our analyses, we found that the inductive reasoning based on the paradima-tic example whose justification is founnded en a local deductive reasoning is the most important characteristics in our textbooks. We also found that some propositions on the properties of various quadrangles impose a deductive reasoning on primary students, which is very difficult to them. The inductive reasoning based on enumeration is used in a few cases, and analogies based on the similarity between the mathematical structures and the concrete materials are frequntly found. The exposition based en a paradigmatic example, which is the most important characteristics, have a problematic aspect that the level of reasoning is relatively low In Miyazaki's or Semadeni's respects. And some propositions on quadrangles is very difficult in Piagetian respects. As a result of our study, we propose that the level of reasoning in primary mathematics is leveled up by degrees, and the increasing levels are following: empirical justification on a paradigmatic example, construction of conjecture based on the example, examination on the various examples of the conjecture's validity, construction of schema on the generality, basic experiences for the relation of implication.
This Studies on 20th century fashion was carried out according to the classification of the times as made up Belle Epoque, New Look, Big Look, and Body-Consciousness. Aside from research in literatures, research into 20th fashion also include the analysis of photo had been taken from various collection. The enlargement beauty of costume had been place under the aesthetic category and, by interpreting the analogies of presented in aesthetic consciousness. the true nature of the voluminous enlargement in costume had been traced. To conclude, this study finds there are between the aesthetic characteristic differences in the aesthetic consciousness of the costumes in history and the present-day fashion. The aesthetic consciousness of the voluminous enlargement of the present-day was found to have the following characteristic ; (1) History-oriented tradition (2) Metaphorica autonomous (3) Freedom of breaking with restriction (4) Dismantling the irregularity (5) Hedonistic play instinct (6) Psychological compensation (7) Sexual sensuality (8) Ideal of the times (9) Aesthetic ornament (10) Feeling of satisfaction through self-enlargement. From list above. metaphorical sensuality, aesthetic ornament and the feeling of satisfaction driven by Self-Enlargement are the same characteristics found in the aesthetics consciousness of the voluminous enlargement of the costume In history in that they are the aesthetic consciousness above time and space. It is metaphorical sensuality which transform woman's body in to distorted images as influenced by both capitalism and materialism in the last 20 years. These are represented by the sadomasochistic and fetish images as the woman's metaphorical voluminous enlargement is apparently becoming more positive, radical and obscene especially in terms of expression.
Opposed to following the contours of the human body, the voluminous enlargement in costume, which characterizes the distinguished enlargement in space rather than the contour of human body, mean the enlargement aspect involving the vertical protrusion and the expansion of shape and volume as well as the extension of length. The costume enlargement as a different method of expression is a symbol showing a meaning of something and an aesthetic expression containing man's will. This voluminous enlargement of costume, as an aesthetic expression, has different formative characteristicsand immanent meanings according to ideals and thoughts as well as social and cultural background of each age Accordingly, the aesthetic consciousness also differaccording to the change of the times. To study the aesthetic consciousness of costume's voluminous enlargement, focous had been given to milieus that show comparatively conspicuous voluminous enlargement Periods that have been subjected to this study include costumes of the ancient Egypt, the Gothic period in the Middle age, the Renaissance, the Baroque Rococo of the recent times, and the modern era (Empire, Romantic, Art Nouveau. etc) With focus given to the principle of design obtained through this study were used to analyze the aesthetic characteristics, Futhermore, based on the spirit of the times and the socio-cultural symbolism, research on immanent meanings, as supported by objectivity and universal validity, was also made, the enlargement beauty of costume had been placed under the aesthetic category and, by interpreting the analogies of presented in aesthetic consciousness, the true nature of the voluminous enlargement in costume had been traced. To Conclude, the aesthetic consciousness of the voluminous enlargement of the costumes in history was found to have following characteristics: (l) Metaphorical (2) Unlooked-for irregularity (3) feeling of satisfaction driven by self-enlargement (4) Dignified sublimity (5) Symbol of wealth and class (6) Ceremonial dignity (7) Tradition of the nobility (8) Aesthetic ornament (9) Ideal contour of the body
Journal of the Korean Society of Clothing and Textiles
/
v.16
no.3
s.43
/
pp.209-221
/
1992
A standpoint art's style, present-day Western dress was establishing a systematic, theoritical value in sphere of fine art's meaning & it's expression on the modern fashion. For this reason, I selected Impressionism (a broad senes: be included Impressionism, Neo-Impressionism, Post-Impressionism) that was designated 'the revolution of color'. In the previous paper, 1 already discussed about the modern fashion under the influence of the color of Impressionism, from this study, Neo-Impressionism's techniques & it's influence on the modern fashion was investigated. The Impressionists had purposely used uneven brushwork & a vivid palette to transmit the intensity & immediacy of nature, whereas the Neo-Impressionists utilized methodically applied dots, a technique commonly described as Pointillism, to achieve their rationalist goal of eliminating the fugitive & the casual in order to seize a more fundamental reality. Of all them, Seurat had already become interested in the posible analogies between science, music & psychology on the on hand & art on the other, and scientist's books & articles offered a wealth of theoretical support. 1'articulary, it was corroborated that effects of sadness, calm, or happiness could be achieved through manipulation of color & design. The conclusions are as follow: 1. Through the modern fashion, a space between pattern & pattern was reflected unstable balances & harmonies, that, Neo-Impressionist emphasized the distinction between outdoor & subject, between elaborately clothed & nude figures, corresponded to modern fashion's pattern. 2. The modern fashion was presented a small border or band within the pattern itself which colors complemented those of the adjacent pictorial surface and mediated between the painted image & its enclosure.
This Study aims at searching the meanings in the creative process of Ronchamp chapel by analyzing the drawings of the site, plan, section and elevation in chronological order. The origins of his architectural creation are identifying with the site(memories of Acropolis), analogies of crab shell, air plane wing and hydraulic dams. The architect informed himself about the site ,the tradition of pilgrimage associated with the place, and its devotion to the Holy Virgin; he looked into the rituals of the Catholic religion, spoke with ecclesiastics; he studied and annotated a monograph devoted to the site of Ronchamp. Also ,the plan required that it be possible to collect rainwater, since such was rare on the hill. In the first design phase, overall conception of building was carried out in the sketches and drawings from June 1950 to November 1950. The second phase in the elaboration of the project was from January 1951(at which time preliminary project was presented to the Commission of Holy Art) to April 1953. The modifications made were in response to opinions expressed by the patrons. The building moved to east and gargoyle moved to west, then, the elevations of north and west were changed. He designed the artificial land and bell tower from the first phase even after the beginning of construction ,he wanted them to be built .But they were not constructed, so the outer space for rituals of religion had no tension. I concluded that Le Corbusier had gifted ability and efforts to create spontaneous birth(after incubation) of the whole work, and the execution of drawings itself. He devoted himself to accomplish the project in spite of the change in situation or by clients.
Proceedings of the Botanical Society of Korea Conference
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1987.07a
/
pp.191-211
/
1987
Common usage of the concept of juvenility implies that there is one physiological phase, the juvenile phase, which manifests itself in the various morphological and physiological phenomena observed in juvenile higher plants. The juvenile phase is often defined as that time from seed germination until the plant attains the ability to flower regulating such behaviour. This definition precludes plants from flowering in the juvenile phase. It is of major interest, therefore, to identify the physiological controls(Bluehreife) regulating such behavior. The length of the juvenile period in higher plants ranges from one year to over 60 years in different species. The long juvenile period of seedling is the main cause of the long duration of the breeding process. I determined the length of the juvenile period in various plants and its control of phase changes in natural system in relation to factors such as plant size and age, shoot morphology, apex size, root system and phytohormonal and nutritional status is reviewed. From the own experimental and observational evidence available it appears that both hormonal and nutritional factors can be involved in control of juvenility but that a specific juvenile or flowering hormone is not involved. Grafting, ringing, scoring, root pruning and fertilization have been used to accelerate flowering, but in most cases these cultured treatments are only successful on plants that were passed the juvenile phase. It is suggested that there are intrinsic difference between the meristematic cells of the apieces of juvenile and adult shoot, which are thus determined with respect to there development potentialities. The problems associated with the maintenance of the determined state through mitosis are discussed. The properties of transitional forms of Ribes nigrum L. intermediate between the juvenile and adult phase, are descrived and there implications discussed. Analogies are drawn between juvenile phenomena in woody perennials and in herbaceous species.
The present work is devoted to study the interaction of $\beta$-aroylacrylic acid derivative (3) with malononitrile in (DMF) in the presence of piperidine and/or ammonium acetate, then using the formed compounds as starting materials for synthesizing fused and isolated heterocyclic systems. It has been established that the $\beta$-aroylacrylic acid (3) reacts with malononitrile in (DMF) in the presence of piperidine as a catalyst with the formation of 4H-pyran derivative (4). By changing the catalyst into ammonium acetate, pyridine derivative (5) has been obtained. Also the N-maleamic acid derivatives (19) and (27) have been synthesized via the interaction of (4) and (5) with maleic anhydride. The purpose of this step is to study the behavior of the formed maleamic acid derivatives – as analogies of $\beta$-aroylacrylic acids – towards different active methylene compounds under Michael addition reaction.
The Journal of Korean Institute of Communications and Information Sciences
/
v.39B
no.3
/
pp.143-150
/
2014
In this paper, we propose an energy efficient and delay guaranteed node scheduling scheme inspired by biological systems, which have gained considerable attention as a computing and problem solving technique. With the identification of analogies between cellular signaling systems and WSN systems, we formulate a new mathematical model that considers the networking challenges of WSNs. The proposed bio-inspired algorithm determines the state of the sensor node, as required by each application and as determined by the local environmental conditions and the states of the adjacent nodes. A control analysis shows that the proposed bio-inspired scheme guarantees the system stability by controlling the parameters of each node. Simulation results also indicate that the proposed scheme provides significant energy savings, as well as reliable delay guarantees by controlling the states of the sensor nodes.
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