• Journal of Ginseng Research
• /
• v.47 no.6
• /
• pp.784-794
• /
• 2023

Background: ginsenoside Rg5 is a rare ginsenoside with known hypoglycemic effects in diabetic mice. This study aimed to explore the effects of ginsenoside Rg5 on skin wound-healing in the Lepr^db/db mutant (db/db) mice (C57BL/KsJ background) model and the underlying mechanisms. Methods: Seven-week-old male C57BL/6J, SLC7A11-knockout (KO), the littermate wild-type (WT), and db/db mice were used for in vivo and ex vivo studies. Results: Ginsenoside Rg5 provided through oral gavage in db/db mice significantly alleviated the abundance of apoptotic cells in the wound areas and facilitated skin wound healing. 50 μM ginsenoside Rg5 treatment nearly doubled the efferocytotic capability of bone marrow-derived dendritic cells (BMDCs) from db/db mice. It also reduced NF-κB p65 and SLC7A11 expression in the wounded areas of db/db mice dose-dependently. Ginsenoside Rg5 physically interacted with SLC7A11 and suppressed the cystine uptake and glutamate secretion of BMDCs from db/db and SLC7A11-WT mice but not in BMDCs from SLC7A11-KO mice. In BMDCs and conventional type 1 dendritic cells (cDC1s), ginsenoside Rg5 reduced their glycose storage and enhanced anaerobic glycolysis. Glycogen phosphorylase inhibitor CP-91149 almost abolished the effect of ginsenoside Rg5 on promoting efferocytosis. Conclusion: ginsenoside Rg5 can suppress the expression of SLC7A11 and inhibit its activity via physical binding. These effects collectively alleviate the negative regulations of SLC7A11 on anaerobic glycolysis, which fuels the efferocytosis of dendritic cells. Therefore, ginsenoside Rg5 has a potential adjuvant therapeutic reagent to support patients with wound-healing problems, such as diabetic foot ulcers.

• Pediatric Infection and Vaccine
• /
• v.30 no.3
• /
• pp.165-172
• /
• 2023

Pyogenic liver abscess is a rare condition in healthy children in developed countries, but it can have severe consequences if diagnosis and treatment are delayed. Staphylococcus aureus is the most commonly identified cause of pyogenic liver abscess worldwide, while Klebsiella pneumoniae is the predominant causative agent in Korea. However, cases of pyogenic liver abscess caused by anaerobic bacteria have also been rarely reported. A 14-year-old boy presented to the emergency room with fever and pain in the upper right abdomen. He exhibited tenderness in the right upper quadrant, elevated white blood cell count, anemia, and elevated liver enzyme levels. Abdominal computed tomography with contrast revealed pyogenic liver abscess. The patient underwent percutaneous catheter drainage for two weeks and received a 4-week course of antibiotic therapy. Prevotella intermedia, an anaerobic bacterium commonly found in the oral flora, was isolated from the drained pus. However, no evidence was found suggesting that the infection originated from a dental source. This case highlights the importance of considering the possibility of pyogenic liver abscess even in otherwise healthy children.

• Korean Journal of Medicinal Crop Science
• /
• v.9 no.1
• /
• pp.26-32
• /
• 2001

The contents of chemical components such as total nitrogen, total amino acid and vitamin C were somewhat higher in $N_2$ gas treatment at $10^{\circ}C$ for 3 hours than those of other treatments. However, the contents of tannin and chlorophyll were slightly lower than that in the other treatments. The content of ${\gamma}-Aminobutyric$ acid with $N_2$ gas treatment was higher $1.5{\sim}6$ times with values of $51{\sim}205mg/100g$ than in control (35mg/100g). The scores of sensory test was not different between $N_2$ gas treatment for 3 hours and control. The contents of chemical components such as total nitrogen, total amino acid including theanine and caffeine were slightly higher in $N_2$ gas treatment at $20^{\circ}C$ for 3 hours than those of other treatments. However, the contents of tannin and vitamin C were slightly lower than those of other treatment. The content of GABA in tea leaves treated with $N_2$ gas was higher $2.5{\sim}7$ times with values of $85{\sim}225mg/100g$ than in control (35mg/100g). The sensory test was lower in $N_2$ gas treatment($76.3{\sim}78.1$ point) than in control(80.4 point). The contents of chemical components were not different between $N_2$ gas treatment at $30^{\circ}C$ for 3 hours and control. Whereas the contents of chemical components were somewhat lower in $N_2$ gas treatment for 1 hour and 5 hours than in control. The content of GABA in tea leaves treated with $N_2$ gas was higher $3{\sim}7$ times with values of $115{\sim}217mg/100g $than in control(35mg/100g). The sensory test was lower in $N_2$ gas treatment ($74.3{\sim}78.4$ point) than in control(80.4 point). Consequently, tea mading within 5 time $N_2$ gas treatment at $10^{\circ}C$ or 3 time $N_2$ gas treatment at 20, $30^{\circ}C$ after plucking was considered to be the best green tea in terms of functional nature as well as taste nature.

• Journal of Korean Society of Water and Wastewater
• /
• v.28 no.6
• /
• pp.623-634
• /
• 2014

This study examined a feasibility of coagulation as post-treatment to remove sulfide and phosphorus for the effluent of anaerobic fluidized bed reactor (AFBR) treating domestic wastewater. Removal efficiencies of sulfide, phosphorus and COD by coagulation were not affected by pH in the range of 5.9 to 7.2. Alkalinity requirement could be estimated by the amount of $Fe^{3+}$ to form $Fe(OH)_{3(S)}$ and to remove sulfide and phosphorus. At coagulant aid dosage of 2 mg/L, anionic polymer showed best results regarding size and settleability of flocs. Sulfide removal for the AFBR effluent at the $Fe^{3+}/S^{2-}$ ratio of 0.64, close to the theoretical value of 0.67 found with a synthetic wastewater, was only 75.2%. One of the reasons for this high $Fe^{3+}/S^{2-}$ ratio requirement is that the AFBR effluent contains sulfide, phosphorus, hydroxide and bicarbonate which can react with $Fe^{3+}$ competitively. Concentrations of sulfide and phosphorous reduced to below 0.1 and 0.5 mg/L, respectively, at the $Fe^{3+}/S^{2-}$ ratio of 2.0. Average effluent COD of 80 mg/L, mostly soluble COD, was obtained at the dosage 50 mg $Fe^{3+}/L$ ($Fe^{3+}/S^{2-}$ ratio of 2.0) with corresponding COD removal of 55%. For better removal of COD, soluble COD removal at the AFBR should be enhanced. Coagulation with $Fe^{3+}$ removed sulfide, phosphorus and COD simultaneously in the AFBR effluent, and thus could be an alternative process for the conventional wastewater treatment processes where relatively high quality effluent is not required.

• KSBB Journal
• /
• v.23 no.1
• /
• pp.90-95
• /
• 2008

In this study, a combined process of sequential anaerobic-aerobic digestion (SAAD), fluidized-bed bioreactor (FBBR), and ultrafiltration (UF) for the treatment of small scale food waste leachate was developed and evaluated. The SAAD process was tested for performance and stability by subjecting leachate from food waste to a two-phase anaerobic digestion. The main process used FBBR composed of aerators for oxygen supply and fluidization, three 5 ton reaction chambers containing an aerobic mesophilic microorganism immobilized in PE (polyethylene), and a sedimentation chamber. The HRTs (hydraulic retention time) of the combined SAAD-FBBR-UF process were 30, 7, and 1 day, and the operation temperature was set to the optimal one for microbial growth. The pilot process maintained its performance even when the CODcr of input leachate fluctuated largely. During the operation, average CODcr, TKN, TP, and salt of the effluent were 1,207mg/L, 100mg/L, 50 mg/L, and 0.01 %, which corresponded to the removal efficiencies of 99.4%, 98.6%, 89.6%, and 98.5%, respectively. These results show that the developed process is able to manage high concentration leachate from food waste and remove CODcr, TKN, TP, and salt effectively.

• Korean Journal of Agricultural Science
• /
• v.38 no.2
• /
• pp.205-212
• /
• 2011

The potential factors for quality loss of cleaned fresh ginseng and technology to be associated with the improvement of marketability through pre-packaging fumigation were examined. Major microorganisms isolated from fresh ginseng included Botrytis cinerea, and Erwinia sp. Others such as Cylindrocarpon sp., Fusarium spp., Pennicilium spp., Bacillus spp. were also found at relatively low frequency. The bacterial density of vacuum packaged fresh ginseng rapidly increased during simulated marketing. Little correlation between bacterial growth and package swelling was found. In order to improve packaging method of fresh ginseng, pre-packaging treatment of 2-phenylethyl alcohol (PE, 100 uL/L, 4 hr) was examined. The fumigation treatment effectively inhibited the growth of bacteria density and also effective on keeping firmness of ginseng root, especially in cortical portion. The internal gas compositions of plastic container packaged for ginseng were approximately ranged between 6 to 8% $O_2$and 3 to 4% $CO_2$. The $O_2$ level of fumigation treatment was lower than control whereas $CO_2$ level was higher. The upsurge of ethylene evolution I day after simulated marketing was found only in fumigation treatment but it returned to ordinary level at day 2. The sucrose content of 2-PE treatment was significantly reduced at 5 days after simulated marketing but reducing sugars like glucose and fructose remained at higher level. The difference in sugar levels was reduced after 10 days of simulated marketing. The decay of fresh ginseng began at the lateral or fine root, which is weak to physical damage, in general. The epidermis was more damaged. Plastic container packaging with PE fumigation could be an alternative to vacuum packaging, which allows an aerobic environment and prevents anaerobic respiration. Further study of pre-package fumigation is required to improve technology of fresh ginseng marketing.

• Microbiology and Biotechnology Letters
• /
• v.25 no.1
• /
• pp.89-95
• /
• 1997

The current processer of the textile wastewater treatment are mostly consisted of a combination of a physico-chemical and a biological treatment. The overall efficiency of these processes is, however, assessed to be fairly low. It is even worse during the summer season when temperature of the wastewater rises above 40$\circ $C. Therefore, a feasible process of the textile wastewater treatment which can work efficiently at higher temperatures was investigated in this work. We used a bench scale reactor consisted of one 4 liter anaerobic and one 8 liter aerobic tank, and the thermophilic symbiotic PVA degraders, Pasteruella hemolytica KMG1 and Pseudomonas sp. KMG6 that had been isolated in our laboratory. In the preliminary flask experiments, we observed that the thermophilic symbiotic PVA degraders could not grow in the wastewater substrate. Then, we isolated the mutant strains by acclimating the KMG1and KMG6 strains to the wastewater medium. The mutant symbionts (KMG1-1 and KMG6-1) were isolated through 6 times successive transfers into the fresh wastewater medium after 5 days culture for each. The mutant strains obtained grew well in the mixed medium composed of 75% wastewater and 25% synthetic medium, and supplemented with 0.5% PVA as a sole carbon source. During the culture for 14 days at pH 7.0 and 40$\CIRC $C, the bacteria assimilated about 89% of the added PVA. The symbionts degraded equally well all the PVA substrates of different molecular weight (nd=500~30000). In contrast to the flask experiments, in the reactor system the mutant strains showed very low levels of the PVA and COD removal rates. However, the new reactor system with an additional aerobic tank attained 82% removal rate of COD, 94% of PVA degradation and 71% of color index under the conditions of 5% inoculm on the tank 2, incubation temperature of 40$\circ $C, dissolved oxygen level of 2~3 mg/l and retention time of 30 hours. This result ensures that the process described above could be an efficient and feasible treatment for the PVA contained textile wastewater at higher temperatures.

• Environmental Engineering Research
• /
• v.20 no.1
• /
• pp.79-87
• /
• 2015

To treat leather industry wastewater (LIW) containing high nitrogen concentration, eight aerobic denitrifiers were isolated from sludge existing in an LIW-treatment aeration tank. Among them, one strain named as KH8 had showed the great ability in denitrification under an aerobic condition, and it was identified as Pseudomonas aeruginosa R12. The aerobic denitrification ability of the strain KH8 was almost comparable to its anaerobic denitrification ability. In lab-scale aerobic denitrifications performed in 1-L five-neck flasks for 48 hr, denitrification efficiency was found to be much improved as the strain KH8 held a great majority in the seeded cells. From the nitrogen balance at the cell-combination ratio of 10:1 (the strain KH8 to the other seven isolates) within the seeded cells, the percentage of nitrogen loss during the aerobic denitrification process was estimated to be 58.4, which was presumed to be converted to $N_2$ gas. When these seeded cells with lactose were applied to plant-scale aeration tank for 56 day to treat high-strength nitrogen in LIW, the removal efficiencies of $COD_{Cr}$ and TN were achieved to be 97.0% and 89.8%, respectively. Under this treatment, the final water quality of the effluent leaving the treatment plant was good enough to meet the water-quality standards. Consequently, the isolated aerobic denitrifiers could be suitable for the additional requirement of nitrogen removal in a limited aeration-tank capacity. To the best of our knowledge, this is the first report of aerobic denitrifiers applied to plant-scale LIW treatment.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.7
• /
• pp.964-969
• /
• 2006

The efficacy of garlic extract and $PGF_2{\alpha}$ in the treatment of endometritis in cows was evaluated. A total of 26 parous cows affected with endometritis were randomly allocated into three groups. Group I (n = 10) animals were infused (IU) with 10 ml garlic extract mixed with 90 ml normal saline, three times at 12 h interval starting from the day of estrum, whereas the animals of Group II (n = 10) were treated with a single injection of $PGF_2{\alpha}$ (25 mg Lutalyse) on the $10^{th}$ to $12^{th}$ day after estrus, and group III (n = 6) remained as control. Cervico-vaginal mucus (CVM) was collected from each animal at pre- and post-treatment estrus and subjected to white side test, pH determination and total bacterial load. The clinical recovery of cows was assessed by negative white side test reaction, pH value and total bacterial count of CVM at subsequent estrus. The recovered animals were inseminated with frozen-thawed semen twice at 12 h intervals and pregnancy was confirmed at 45-60 days following insemination. A significant decline (p<0.05) in pH of CVM was observed in both the treatment groups at subsequent estrus. After treatment there was a significant (p<0.05) reduction in bacterial load, whereas, it was increased in control group. A total number of 65 isolates were identified in CVM samples comprising mostly of facultative anaerobic bacteria. Plasma $T_4$ and $T_3$ concentrations were increased in all the treated animals, whereas, a decline was observed in cortisol levels following treatment. The overall conception rate was 50% in treated groups as compared to nil pregnancy in the control.

• Journal of Korean Society on Water Environment
• /
• v.20 no.5
• /
• pp.437-446
• /
• 2004

To develop sewage treatment apparatus by natural purification method, the sewage treatment plant that consisted of aerobic and anaerobic plot was constructed. And then, the effects of treatment conditions on the removal of pollutants in the relation to sewage loading, sewage injection method and season according to the pebble kind. Removal rate of BOD and COD according to the sewage loading in effluent were over 95 and 77%, respectively. Removal rate of nitrogen in treated water by aerobic plot and effluent using sand were about 22~40 and 49~75%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% as filter media in comparison with using sand were about 7~25 and 16~23%, respectively. Removal rate of phosphorus in treated water by aerobic plot and effluent using sand as filter media were about 30~36 and 52~56%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% in comparison with using sand as filter media were about 11~40 and 12~45%, respectively. Removal efficiency of BOD and COD according to the intermittent injection method of sewage were slightly decreased, but those of nitrogen and phosphorus were little varied in comparison with continuous injection method. Removal efficiency of BOD and COD in winter in comparison with the others were little varied, but those of nitrogen and phosphorus slightly decreased.