• 한국식품과학회지
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• 제12권2호
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• pp.88-96
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• 1980

아미노-카르보닐 반응에 대한 반응 조건을 검토하고 반응성에 미치는 아미노산(酸)의 영항에 대하여 검토한 결과 다음과 같은 결과를 얻었다. 1. 아미노-카르보닐 반응은 알칼리성이 강할수록 melanoidin 생성량은 크게 증대되었으며, 아미노산의 등전점 이상에서 반응 속도는 더욱 증가되는 것으로 나타났다. 2. 당(糖)과 아미노산(酸)의 몰 분비(分比)가 1:1일 때까지 melanoidin 생성량은 직선적으로 증가되었으며, 당과 아미노산(酸)은 1:1로 결합함을 알 수 있었다. 3. 아미노-카르보닐 반응은 시간과 반응 온도에 높은 의존성을 보였으며, 동(同) 몰의 glucose-glycine 계(系)에서의 활성화 에너지는 37.5Kcal/mole이었다. 4. 사용된 아미노산(酸) 중(中)에서 glycine, lysine 및 $\beta$-alanine이 당과의 반응성이 현저하게 높았으며, 산성(酸性)아미노산(酸)의 반응성은 아주 낮았다. 따라서 산성(酸性) 아미노산(酸)의 측쇄(測鎖)의 카르복실기(基)는 아미노기(基)의 반응성에 가장 저해(沮害) 결과(結果)를 나타내었다. 5. 사용된 당류(糖類)의 반응성은 다음의 순서로 나타났다. Xylose>arabibose>fructose>glucose>maltose>lactose.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.635-638
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• 2011

A simple and efficient method for the synthesis of $\beta$-amino carbonyl compounds by one-pot three-component Mannich reaction of acetophenone, aromatic aldehydes and aromatic amines using a carbon-based solid acid (CBSA), as an effective and reusable catalyst, is described. The present methodology offers several advantages such as simple procedure with an easy work-up, shorter reaction times, and high yields.

• 한국식품과학회지
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• 제37권5호
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• pp.812-815
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• 2005

청국장 점질물인 PGA의 갈변 및 갈변 반응물질의 분획에 따른 갈변도와 전자 공여능에 따른 항산화력을 알아보았다. PGA의 갈변은 자체적으로는 갈변이 잘 일어나지 않고, 염기적 조건 하에서 glucose을 첨가하고 가열하면 갈변이 급격하게 일어나며 전자 공여능 또한 높게 나타났다. 염기적 조건 하에서 얻은 PGA 갈변 반응 물질(Glu-PGA, pH 8.2)을 Sephadex G-50을 이용하여 분획하고, 분획에 따른 갈변도 및 DPPH를 이용한 전자 공여능을 측정하였다. 분획된 갈변 반응 물질들은 비교적 광범위한 갈변도 및 전자 공여능을 보여 주었다. 이들 중 갈변도 및 전자 공여능이 상대적으로 높은 F-7과 F-20의 UV-VIS 흡수대에 있어서는 F-20은 멜라노이딘의 흡수대인 260-320nm에 걸쳐 완만한 흡수곡선을 나타내었고, F-7은 270nm 부근에서 특징적인 최대 흡수대를 나타내었다. 두 분획 물질의 분자량은 SDS-PAGE로 분석한 결과 F-7은 대략 35kDa 이상으로 나타났고, F-20은 SDS-PAGE상에서 뚜렷한 크기는 알 수 없었으나, F-7 보다는 비교적 저분자량을 갖는 물질이라는 것을 알 수 있었다.

• 대한화학회지
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• 제55권4호
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• pp.644-649
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• 2011

Polyaniline/$SiO_2$ 촉매를 이용하여, acetophenone, aromatic aldehydes와 aromatic amines을 에탄올 용매 속에서 반응시켜서 다양한 various ${\beta}$-amino ketones을 one-pot mannich 반응을 수행하였으며, 이 반응을 위해 silica가 충진된 여러 가지 종류의 wt% polyaniline을 화학적인 산화방법에 의해 합성하였다. 합성한 촉매는 thermal analysis(TG-DTA), X-ray diffraction (XRD), scanning electron microscopy(SEM) energy dispersive spectroscopy(EDS), 및 Fourier transform infrared spectroscopy (FT-IR) 방법으로 확인하였으며, 촉매의 용매에 대한 안정도 UV-Visible spectroscopy로 측정하였다. Polyaniline/$SiO_2$ 촉매를 이용하는 합성 방법은높은 수율로 얻어지며, work up이 쉽고, 독성이 없으며, 쉽게 회수하여 재사용이 가능하다.

• Archives of Pharmacal Research
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• 제15권2호
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• pp.138-141
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• 1992

The new N-aryl-benz[f]-indole-4, 9-dione derivatives were synthesized via in tramolecular cyclization when triethylamine was employed as a base for the reaction of 2-chloro-3-$(\alpha$-cyano-$\alpha$-ethoxycarbonyl-methyl)-1, 4-naphthoquinone and arylamines.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.320-323
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• 1998

Lysozyme was covalentyl conjugated with galactomannan through a amino-carbonyl reaction between the lysine $\varepsilon$-amino groups of lysozyme and the reducing ends of galactomannan at a relative humidity of 79% and 6$0^{\circ}C$. The resulting lysozyme-galactomannan conjugate (LGC) was investigated for its antibacterial activity against Escherichia coli. Lysozyme alone did not exhibit antibacterial activity against E. coli. in contrast , significant bactericidal effect was observed for LGC, depending on the reaction temperature. The degree of conjugation between lysozyme and galactomannan was dependent on the incubation time, which affected the antibacterial efficiency against E. coli. This study demonstrated that the amino-carbonyl reaction between lysozyme and galactomannan could be a potential tool to modify lysozyme toward broadening its antibacterial spectrum to Gram-negative bacteria.

• Bulletin of the Korean Chemical Society
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• 제32권5호
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• pp.1703-1708
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• 2011

ZSM-5-$SO_3H$ efficiently catalyzed the one-pot three-component Mannich reaction of aldehydes, anilines, and ketones. ${\beta}$-Aminocarbonyl compounds were obtained in reasonable yields and excellent stereoselectivities when the reaction was carried out at room temperature under solvent-free conditions. Simple experimental conditions and product isolation procedure makes this protocol potential for the development of clean and environment-friendly strategy for the synthesis of ${\beta}$-amino-ketones. The catalyst was recovered and reused for subsequent runs.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1102-1108
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• 1997

Proteins and polysaccharides confer distinct functional properties in food systems. This research was attempted to improve emulsion properties of casein by protein-polysaccharide conjugation, in which alginates with various molecular weights were employed as polysaccharide sources. Casein-alginate mixtures were conjugated by the amino-carbonyl or Maillard reaction at 6$0^{\circ}C$ and 79% relative humidity. The resulting casein-alginate conjugates were tested for their emulsion activity and emulsion stabilizing properties. In general, the emulsion stability of casein-alginate mixture greatly increased due to the amino-carbonyl reaction between casein and alginates, whose magnitude depended on the molecular weight of alginate, weight ratio of casein to alginate and incubation time. It was also found that thermal stability and pH stability were markedly improved by the casein-alginate conjugation.

• 한국식품영양학회지
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• 제11권5호
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• pp.499-505
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• 1998

The purpose of this study was to ivestigate the structural characteristics of waste soulble browning reaction prodcuts (WS-BRPs) isolated from Korean red ginseng. They all showed the maximum absorbances at near 280 nm. Their IR spectra suggested the presence of hydroxyl, amide carbonyl and aliphatic methine groups. From sugar analysis it was identified that L and S-1 contained two kinds of suggar, glucose and xylose, and S-2, a fructose together with glucose and xylose. Thirteen different amino acids were identified in L. Ten amino acids from S-1 and seven amino acids from S-2 were identified using Auto Amino Acid Analyzer. Glycine, serine and glutamic acid in S-1 and one unknown amino acid and glycine in S-2 were detected as the major amino acids, respectively. From the 1H-and 13C-NMR spectra, it was identified that a number of sugar moieties, carbonyl and carbon double bonds (only in S-2) were contained in the three WS-BRP components. Approximate number of sugar moiety of L, S-1 and S-2 was determined to be 8∼10, 9∼11 and 4∼5, respectively. Contents of nitrogen, carbon and hydrogen showed L>S-1>S-2.

• Bulletin of the Korean Chemical Society
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• 제31권3호
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• pp.553-554
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• 2010