Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Characteristics of Browning Reaction Products of Glucose-Poly-${\gamma}$-Glutamate (GIu-PGA) obtained from Amino-carbonyl Reaction

Amino-carbonyl 반응에 의한 glucose-poly-${\gamma}$-glutamate (Glu-PGA) 갈변 반응물질의 항산화적 특성 연구

  • Published : 2005.10.31
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Abstract

Effects of poly-${\gamma}$-glutamate (PGA) on antioxidative characteristics of amino-carbonyl reaction products of glucose were investigated. Rapid browning reaction was observed under heat and alkaline condition (pH 8.2). Browning products were separated by Sephadex G-50, and brown color intensity and electron-donating ability of DPPH (2,2-diphenyl-1-picrylhydrazyl) of each fraction were measured. Fraction-7 (F-7) and -20 (F-20) showed high DPPH scavenging values. UV-VIS absorption spectrum of F-20 was similar to melanoidin peak, and F-7 showed maximum absorption peak at 270 nm. Molecular weight of F-7 was over 35 kDa as determined by SDS-PAGE. Although F-20 could not be measured on SDS-PAGE, its size was smaller than F-7.

청국장 점질물인 PGA의 갈변 및 갈변 반응물질의 분획에 따른 갈변도와 전자 공여능에 따른 항산화력을 알아보았다. PGA의 갈변은 자체적으로는 갈변이 잘 일어나지 않고, 염기적 조건 하에서 glucose을 첨가하고 가열하면 갈변이 급격하게 일어나며 전자 공여능 또한 높게 나타났다. 염기적 조건 하에서 얻은 PGA 갈변 반응 물질(Glu-PGA, pH 8.2)을 Sephadex G-50을 이용하여 분획하고, 분획에 따른 갈변도 및 DPPH를 이용한 전자 공여능을 측정하였다. 분획된 갈변 반응 물질들은 비교적 광범위한 갈변도 및 전자 공여능을 보여 주었다. 이들 중 갈변도 및 전자 공여능이 상대적으로 높은 F-7과 F-20의 UV-VIS 흡수대에 있어서는 F-20은 멜라노이딘의 흡수대인 260-320nm에 걸쳐 완만한 흡수곡선을 나타내었고, F-7은 270nm 부근에서 특징적인 최대 흡수대를 나타내었다. 두 분획 물질의 분자량은 SDS-PAGE로 분석한 결과 F-7은 대략 35kDa 이상으로 나타났고, F-20은 SDS-PAGE상에서 뚜렷한 크기는 알 수 없었으나, F-7 보다는 비교적 저분자량을 갖는 물질이라는 것을 알 수 있었다.

Keywords

References

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