• 한국조경학회지
• /
• 제32권4호
• /
• pp.49-58
• /
• 2004

This study is aimed to define and classify GREEN TOURISM (GT) resources as the basis for rural sustainable development, and to analyze the current conditions of GT and applicable resources in Korea. For these purposes, a field survey about 24 rural villages in Japan and Korea is applied, accompanied by a questionnaire survey of 30 specialists, as well as a literature review. The results of this study are summarized as follows. The concept of GT has 3 important points: interchange between rural and urban residents, staying in villages, and residents' participation. GT resource are divided into 4 types: Agricultural, Ecological, Historical and Living resources in a space and activity program. In addition, the GT resources in Japan are more quantitatively abundant than in Korea, but Korean agricultural and ecological resources were more abundant relatively. According to the several methods above, 18 space and 14 activity programs were classified. Lastly, this study analyzed the applicable 32 Korean resources using the specialists' questionnaire survey. Among the space resources, ecological landscape, farm produce, farm-land, water, and historical landscape have higher applicability, but the applicability of sports, event, commemoration, and divine space are lower. Among the activity programs, farm produce, animal, plant, food&beverage, and living appliance have higher applicability.

• 농촌계획
• /
• 제4권1호
• /
• pp.40-55
• /
• 1998

The objectives of this study are to 1)research the importance of direct trade of agricultural products between farmers and consummers, 2)invest the trade process of agricultural products, 3)survey the patterns of direct trade of agricultural products, 4)find out the situations of direct trade of agricultural products, 5)suggest the directions to improve the direct trade of agricultural products. The data were collected from Taegu city, Taechun city and Kyungsang poop do for this study. The major findings of this study to improve the direct trade of agricultural products between urban and rural are as follows; First, it is very important that producers and consummers have common sense to the direct trade between urban and rural. Second, local government related the trade of agricultural products should positively take parts in the direct trade of agricltural products between producers and consummers in administration and finance. Thirth, non-profit organizations above other organizations in urban and rural must lead the direct trade of agricultural products between urban and rural. Fourth, the markets for direct trade of agricultural products are to be opened according to basing on consummers'needs to agricultural products. Fifth, the engaged farmings between producers and consummers are to be more positive by significant relationship( promised brothers and sisters) between rural and urban. Sixth, people who moved to urban from rural should be finked out in each community(in rural), they can buy the agricultural products in the community that they were born. Seventh, The communities in rural must produce the specific agricultural products in each community.

• Journal of Microbiology and Biotechnology
• /
• 제24권10호
• /
• pp.1421-1426
• /
• 2014

Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an aroA gene ($aroA_{A.\;metalliredigens}$) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new $aroA_{A.\;metalliredigens}$ gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.

• Journal of Plant Biotechnology
• /
• 제41권3호
• /
• pp.107-115
• /
• 2014

식물지방은 주로 종자에서 생산되는데 인류에게 필수 지방산을 공급하는 식품 뿐 아니라 바이오디젤 등 산업원료로 그 이용가치가 크다. 식물지방의 수요 증가에 따른 식물지방의 생산증대가 필요하다. 식물지방을 종자 이외의 바이오매스가 큰 식물의 잎에서 생산한다면 식물지방 생산 증진이 가능할 것이다. 잎은 지방을 생산하는 기관이 아니며 주로 광합성을 통해 탄소를 고정하여 다른 기관으로 탄소를 공급하는 기능을 하고 있어 지방을 생산 축적하는 기관으로 전환하는 데는 많은 고려가 필요하다. 그럼에도 불구하고 최근 지방합성 조절인자인 WRI 유전자, 지방을 생성하는 acyltransferase인 DGAT 유전자의 발현에 의해 잎에서 지방을 합성할 수 있었다. 또한 지방의 분해를 안정화하는 올레오신 단백질의 추가 도입으로 잎에서 건조중량당 15%의 중성지방 생산을 보여 잎에서 지방생산 가능성을 보여주었다(Vanhercke et al. 2014). 앞으로 바이오매스에서 지방을 생산하는 연구가 활발할 것으로 예측되며 이 기술을 식용작물이 아닌 비식용이며 바이오매스가 큰 거대억새 등에 도입하여 농지로 적합하지 않은 열악한 토지 및 간척지 등에 재배하여 실용화한다면 미래 지속 생산 가능 친환경 바이오 원료 생산 자원으로 사용 가능하리라 사료된다.

• 한국환경농학회지
• /
• 제40권4호
• /
• pp.248-259
• /
• 2021

BACKGROUND: Contaminated water was a major source of food-borne pathogens in various recent fresh produce-related outbreaks. This study was conducted to investigate the microbial contamination level and correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water by logistic regression analysis. METHODS AND RESULTS: Agricultural water was collected from 457 sites including surface water (n=300 sites) and groundwater (n=157 sites) in South Korea from 2018 to 2020. Sanitary indicator bacteria (total coliform, fecal coliform, and Escherichia coli) and food-borne pathogens (pathogenic E. coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes) were analyzed. In surface water, the coliform, fecal coliform, and E. coli were 3.27±0.89 log CFU/100 mL, 1.90±1.19 log CFU/100 mL, and 1.39±1.26 log CFU/100 mL, respectively. For groundwater, three kinds of sanitary indicators ranged in the level from 0.09 - 0.57 log CFU/100 mL. Pathogenic E. coli, Salmonella and Listeria monocytogenes were detected from 3%-site, 1.5%- site, and 0.6%-site water samples, respectively. According to the results of correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens by logistic regression analysis, the probability of pathogen detection increased individually by 1.45 and 1.34 times as each total coliform and E. coli concentration increased by 1 log CFU/100mL. The accuracy of the model was 70.4%, and sensitivity and specificity were 81.5% and 51.7%, respectively. CONCLUSION(S): The results indicate the need to manage the microbial risk of agricultural water to enhance the safety of fresh produce. In addition, logistic regression analysis is useful to analyze the correlation between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water.

• 한국식품저장유통학회지
• /
• 제14권6호
• /
• pp.695-701
• /
• 2007

본 연구는 김치제조용 월동배추의 속소금 살포 절임 기계화 작업의 표준화를 위한 것이다. 이를 위해 중대형 김치 공장을 조사하여 속소금 살포에 의한 배추의 기계화 절임의 주요변수를 구하였으며, 그 결과 절임염수 농도, 절임시간, 탈수시간이 주요변수임을 파악하였다. 절임 시 이 변수의 적정값을 구하기 위하여 일련의 실험실 기초실험을 월동배추를 사용하여 수행하였고, 그 실험 결과를 타 계절 배추 절임에 적용 가능성을 확인하기 위하여 타 계절 배추에 대해서도 실험하였다. 마지막으로 실험실 기초실험 결과의 정당성을 확인하기 위하여 공장 규모의 실증실험을 수행하였으며 그 결과 본 연구에서 얻은 결과는 공장 규모의 월동배추 속소금 살포 절임방법으로 권장할 만 함을 확인하였다.

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
• /
• pp.118-119
• /
• 2003

Baculovirus occlusion bodies have been recently engineered to incorporate foreign protein such as the Bacillus thuringiensis (Bt) CrylAc protein for improvement of insecticidal activity. In this study, polyhedrin, cylAc, egfp and crylCa genes were fused to produce occlusion bodies that contain novel recombinant crystal protein by homologous recombination between cylAc and crylCa genes in insect cells. (omitted)

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.134.2-135
• /
• 2003

The plant-pathogenic species S. scabies, S. acidiscabies, and S. turgidiscabies cause the scab disease of potato and produce the phytotoxins, thaxtomins. necl, a gene conferring a necrogenic phenotype, is involved in pathogenicity and physically linked to the thaxtomin A biosynthetic genes. Identification of the pathogenic strains of Streptomyces from soil was performed through the polymerase chain reaction by using specific pathogenicity primer sets derived from the necl gene sequences of Streptomyces smbies. The DNA was extracted from soil using a bead-beating machine and modifications of the FastPrep system. The DNA was suitable for direct use in the PCR. The PCR products showed the bands of approximately 460 bp. This methods can be very usuful in identifying species responsible for scab diseases and studying on the ecology of plant-pathogenic Streptomyces spp.

• 농촌지도와개발
• /
• 제19권3호
• /
• pp.729-755
• /
• 2012

The role of branding has been to differentiate products, but brands have been increasingly applied to organizational image too. The brand image holds a key position by the marketing system in this era. The main purpose of this research is to analyze the case for the development of farm brand. A in-depth interview was conducted to analyze the case for development of farm brand in connection with agricultural products and their business operation. The farms used various stories in the process of develop brand including farm history, cultivation process, product, management, relationship with consumers. The study present the findings divide into brand naming, image, positioning. The farms built trust by consumer relationship with the farm brand. The results of the study provide insights for agricultural marketers who intend to design brand stories that align with their produce.

• 한국동물생명공학회지
• /
• 제35권1호
• /
• pp.2-11
• /
• 2020

Assisted reproductive technologies (ART) merely depend on improving the oocyte maturation and their developmental competence to produce good quality embryos. Oocyte maturation passes through long and complex molecular steps starts from the early embryonic life and ends with sperm fertilization. Oocyte developmental competence can be attained by improving the nuclear and cytoplasmic mechanisms together with some epigenetic maturation. In this review, we highlight the cornerstones of oocyte maturation on both nuclear and cytoplasmic levels. Interfering or supporting these molecular mechanisms would help in the development of novel regulating agents for reproductive performance of humans and livestock species.