• Journal of Dairy Science and Biotechnology
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• v.40 no.4
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• pp.151-162
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• 2022

Although many developed countries (USA, Canada, and several EU countries) allow raw milk cheese to be aged more than 60 days, these countries have strict standards for the aging conditions, such as temperature, of raw milk cheese. Spiking experiments were conducted with Camembert cheese made from raw milk, to assess the microbiological safety of raw milk cheese aged for more than 60 days. We spiked Escherichia coli O157:H7 into raw milk with different inoculation levels (high, medium, and low). Camembert cheese was prepared from the inoculated raw milk, then aged in an incubator for up to 9 weeks (63 days). There were no significant differences in pH and water activity (aW) between uninoculated cheese and cheese samples inoculated with E. coli O157:H7 (p<0.05). The pH and aWof the Camembert cheese decreased throughout the storage period. In conclusion, E. coli O157:H7 did not affect the pH and aW of the cheese samples. Cell counts were conducted every week using the agar-plating method. Inoculated cells were completely eliminated, especially in Camembert cheese, after 60 days, and the reduction rate of cells was much faster in Camembert cheese.

• Safety and Health at Work
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• v.15 no.1
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• pp.42-52
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• 2024

Background: The lack of headforms that accurately reflect the head characteristics of Koreans and the demographic composition of the Korean population can lead to inadequate FFR testing and reduced effectiveness of FFRs. Method: Direct measurements of 5,110 individuals and 3D measurements of 2,044 individuals, aged between 9 and 69 years, were sampled from the data pool of Size Korea surveys based on the age and gender ratios of the Korean resident demographics. Seven head dimensions were selected based on the ISO 16976-2, availability of Size Korea measurements, and their relevance to the fit performance of FFRs. A principal component analysis (PCA) was performed using the direct measurements to extract the main factors explaining the head characteristics and then the main factors were standardized and remapped to 3D measurements, creating five size categories representing Korean head shapes. Lastly, representative 3D headforms were constructed by averaging five head shapes for each size category. Results: The study identified two main factors explaining Korean head characteristics by the PCA procedure specified in ISO 16976-2 and developed five representative headforms reflecting the anthropometric features of Korean heads: medium, small, large, short & wide, and long & narrow. Conclusion: This study developed representative headforms tailored to the Korean population for conducting total inward leakage (TIL) tests on filtering facepiece respirators (FFRs). The representative headforms can be used for TIL testing by employing robotic headforms to enhance the performance of FFRs for the Korean target population.

• Tunnel and Underground Space
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• v.28 no.6
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• pp.596-608
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• 2018

As urban infrastructure is aging, the possibility of accidents due to the failures or breakdowns of infrastructure increases. Especially, aging underground infrastructures like sewer pipes, waterworks, and subway have a potential to cause an urban ground sink. Urban ground sink is defined just as a local and erratic collapse occurred by underground cavity due to soil erosion or soil loss, which is separated from a sinkhole in soluble bedrock such as limestone. The conventional measurements such as differential settlement gauge, inclinometer or earth pressure gauge have a shortcoming just to provide point measurements with short coverage. Therefore, these methods are not adequate for monitoring of an erratic subsidence caused by underground cavity due to soil erosion or soil loss which occurring at unspecified time and location. Therefore, an alternative technology is required to detect a change of underground physical condition in real time. In this study, the feasibility of a novel magnetic resonance based monitoring method is investigated through laboratory tests, where the changes of path loss (S21) were measured under various testing conditions: media including air, water, and soil, resonant frequency, impedance, and distances between transmitter (TX) and receiver (RX). Theoretically, the transfer characteristic of magnetic field is known to be independent of the density of the medium. However, the results of the test showed the meaningful differences in the path loss (S21) under the different conditions of medium. And it is found that the reflection coefficient showed the more distinct differences over the testing conditions than the path loss. In particular, input reflection coefficient (S11) is more distinguishable than output reflection coefficient (S22).

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.239-245
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• 1999

The effect of catalase on in-vitro maturation in porcine oocytes with or without cumulus cells were studied. The maturation rates were not significantly different in medium with and without catalase during in vitro maturation of oocytes. However, the maturation rates of oocytes with cumulus cells were significantly higher (P＜0.05) than in oocytes without cumulus cells regardless of the presence of catalase. On the other hand, the maturation rate in oocytes cultured with cumulus cells for 48 h (57%) was significantly (P＜0.05) higher than in oocytes with cumulus cells for first 24 h period (42%) only. In another experiment, the maturation rate was significantly (P＜0.05) higher in medium containing catalase for last 24 h period only than in medium containing catalase for first 24 h period during in vitro maturation of ooytes with or without cumulus cells. But the oocytes matured to M-II stage were observed at 24 h of culture of oocytes without cumulus cells only. When oocytes with cumulus cells were cultured for 72 h, the maturation rates was significantly (P＜0.05) higher in medium with (79%) than without (65%) catalase during in vitro maturation. These results indicate that cumulus cells are necessary for in vitro maturation of porcine oocytes, catalase have effect according to the addition periods and can prevent aging of porcine oocytes during maturation in vitro.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.302-305
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• 2003

It is difficult to obtain sufficient healthy skin for coverage of a wide area of skin wound. In the skin, an additional population of living epithelial cells is located in the outer root sheath (ORS) of hair $follicles.^{1),2)}$ ORS cells should be a good source of epithelium because they are easily obtainable and patients do not have to suffer from scar formation at donor sites. We modified ordinary primary culture technique for the purpose of solving such problem that epithelial cells have a low propagation and easy aging during culture periods. First of all, we improved primary cultivation methods. In the ordinary primary culture, average yield of human ORS cells was $2\;{\times}\;10^3$ cells/follicle by direct incubation with trypsin (0.1%)/EDTA (0.02%) solution for 15 min at $37^{\circ}C$ but we could obtain about $6.5\;{\times}\;10^3$ cells/follicle by two step enzyme digestion method with dispase (1.2 U/ml) and trypsin (0.1%)/EDTA (0.02%) solution. So we could achieve three times higher primary cultured ORS cell yield. Secondly, we could obtain total $2\;{\times}\;10^7$ cells in serum free medium and even more total $6\;{\times}\;10^7$ cells in modified E-medium with mitomycin C-treated feeder cells during 17 days. Using the cultured ORS cells, and we could make bioartificial skin equivalent in vitro and concluded that ORS cells were progenitor cells for skin epithelial cell.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.131-136
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• 1994

In the present study, in order to make an in vitro model of gastric mucosa for physiological and pharmacological studies, we established two immortalized gastric surface mucous cell lines (GSM06 and GSM10), which produce periodic acid-Schiff (PAS)-and concanavalin A (Con A)-positive glycoproteins, from a primary culture of gastric fundic mucosal cells of adult transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen gene 〔1]. Gastric fundic mucosal cells were isolated as a modification of a previously described method for rats by Schepp et al. (2). The isolated gastric fundic mucosal cells were cultured in DME/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (consisting of 2 mg/1 insulin, 2 mgg/1 transferrin, 0.122 mg/1 ethanolamine and 0.00914 mg/1 sodium selenite) and 10 ng/ml recombinant epidermal growth factor (EGF) in a collagen-coated culture dish. To remove fibroblastic cells from the culture, gastric mucosal cells were incubated in the culture medium containing dispase (25 U/ml) for 24 h. The cells, uncontaminated with fibroblastic cells, were then cloned by colony formation. In our series of three attempts, two cell lines (GSM06 and GSM10) have been established at last. The cells proliferated, attached to the dish ana grew until confluent monolayers were formed, and maintained tight contact with neighboring cells. Both GSM06 and GSM10 cells have now been in culture for more than 9 months with regular passaging. The either cell produced

• Journal of Embryo Transfer
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• v.12 no.2
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• pp.171-179
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• 1997

The objectives of the present study were improvements in the efficiency of developmental rates to morula and blastocyst stages to produce a large number of genetically identical nuclear transplant embryos. The oocytes collected from slaughterhouse ovaries were matured for 24 h and then enucleated and cultured to allow cytoplasmic maturation and gain activation competence. And then the donor embryos were treated for 12 h with 10 $\pi$g /ml nocodazole and 7.5 $\pi$g /ml cytochalasin B to synchronize the cell cycle stage at 26 h after the onset of culture. The blastomeres were transferred into the perivitelline space of the enucleated nocytes and blastomeres and oocytes were fused by electrofusion. The cloned embryos were then cultured in various conditions to allow further development. The age of the recipient(30 vs 40 h) had no significant effect on the fusion rates(82.4 vs 82.1%) and the developmental rates to morula /blastocyst(9.8 vs 11.0%). Effect of Nocodazole treatment on the donor cell cyle synchronization to improve the developmental rates of bovine nuclear transplant embryos was significantly higher than control group(21.4 vs 10.1%, p<0.05). Significant differences were in the percentage of fusion rates(72.9,77.1vs 61.9%) in three types of fusion medium(PBS(+), mannitol and sucrose, p<0.01). The developmental rates of bovine nuclear transplant embryos appeared to be highest in mSOF medium under 5% 0$_2$ condition, but no significant differences were found when compared with TCM199-BOEC and mSOF under two different oxygen ratio(5 and 20%).

• Journal of the Korean Society of Costume
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• v.62 no.1
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• pp.76-89
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• 2012

This study was performed to offer the basic data for the design of farm shoes. 265 Korean female farmers aging between the 40s to the 80s volunteered for this study and we measured 40 items on each foot with the 3D foot scanner. First, the differences between farmers' feet and non-farmers' feet were analyzed. Farmers' feet were thicker in the instep areas, but had lower arch height than non-farmers' feet. In addition, farmer's feet were tilted to the inside. Next, eight factors were extracted among the 40 measuring items, and the classification criteria of the foot shape was analyzed. The important factors were: size of foot length and volume of ankle, malleolus height and size, volume of the front part of ankle, medial & lateral ball width, and vertical size of foot. Third, three clusters according to the foot shapes were categorized by cluster analysis of eight factor scores. Foot type 1 was medium in foot length, big in thickness, large in lateral ball width, small in toe 1 angle, and tilted to the inside. Foot type 2 was long and slim, and big in toe 5 angle. Foot type 3 was short in foot length, medium in volume of the front part of ankle, large in medial ball width, and big in toe 1 angle. Despite its shortness, foot type 3 was thick and showed severe deformation in toe 1. Lastly, the frequency distributions of the foot types in each age group were analyzed. Female farmers of the forties showed high frequency in type 1 and other age groups showed high frequency in type 2. The older female farmers showed higher frequency of type 3.

• The Journal of Industrial Distribution & Business
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• v.11 no.5
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• pp.35-44
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• 2020

Purpose: The purpose of this study is to present a plan to expand the employment of the elderly in Small business as one of the ways to solve the problem of the supply and demand of the company due to the aging population and the problem of the elderly poverty. Research design, data and methodology: The method of this study is a qualitative research method, and the researcher visited a small and medium-sized company directly and collected data by conducting an in-depth interview with a business owner. The interview period was conducted on a total of 15 business owners from November 5, 2013 to November 18, 2013. Results: First, the reason why companies hire the elderly is because they are suitable people, and the reason why they are not employed is because the elderly are not suitable. Second, it was found that the most recruiting paths continued to work after retirement. Third, the strengths of the elderly in their businesses were diligence, integrity, leadership, wisdom, warmth, and skill. Disadvantages the elderly in their businesses include wanting to be treated as an adult, poor productivity, poor accuracy, and health risks. Fourth, in case of hiring the elderly, they were considering convergence with young employees, and it was suggested that there are no difficulties in being an elderly because the companies hiring the elderly use the elderly according to the characteristics of the elderly. Fifth, It is realistic to actively utilize the employment system after retirement. Sixth, it was found that, unlike young people, it is not easy to recruit people on the Internet, so it is necessary to improve the system. Lastly, some industries clearly distinguished between the jobs of the elderly and the jobs of the young, but in many industries, it was desirable to create jobs for the elderly by harmonizing the main and secondary jobs. Conclusion: Ultimately, the work of the elderly in small and medium-sized enterprises should be reborn as a high-quality job that can solve the poverty of the elderly by working as a regular worker in the enterprise, rather than simply working for the elderly.

• Fashion & Textile Research Journal
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• v.10 no.6
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• pp.979-988
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• 2008

The purpose of this study is to analyze the lower body size and forms of married women in their thirties who have experienced pregnancy and parturition. Their measurements derived from the direct anthropometry and indirect photographic measures were compared to those of 20's. Improving consumer satisfaction for missy brand, especially in fitness of skirt and pants, some lower body classification were presented. The results were as follows : 1. The characteristics of lower body showed many differences by individuals especially in depth, girth, length and angle measures rather than height and breadth measures. Many items including index, calculations and angles revealed significant differences compared to those of 20's. Several breadth items, crotch length had distinct increase than those of women in twenties. Flattened buttocks side silhouette from waist to hip, buttocks drooping and decrease of inclination implied the influence of pregnancy, parturition and aging. 2. We hardly find out sizing system differences between missy brand and miss brand. Based on waist and hip girth measurements of subjects, sizing system of missy apparel wasn't suitable for their body size. Excessive drop(hip girth-waist girth) of missy brands, 25~30 cm, was almost the same to the drop of miss brands. 3. To improve the size satisfaction of missy women, we classified the lower body into 5 types according to drop, buttocks angle and hip length. Type 1 was small waist with large buttocks projection, and hip length was close to an average. Type 2 was small waist with medium buttocks projection, and the hip length was close to an average. Type 3 was medium waist, with average projection of the buttocks and hip length. Type 4 was large waist, projection of the buttocks and the hip length were close to an average. Type 5 was large waist, projection of the buttocks was small and the hip length was close to an average.