Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 12 Issue 2
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- Pages.171-179
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- 1997
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Studies on the Cloning of Calves by Nuclear Transplantation I. Effects of Cell Cycle, Fusion Media and Oxygen Concentration on the Developmental Competence
핵이식을 이용한 복제송아지 생산에 관한 연구 I. 세포주기, 융합배지 및 산소분압이 체외발육능에 미치는 영향
Abstract
The objectives of the present study were improvements in the efficiency of developmental rates to morula and blastocyst stages to produce a large number of genetically identical nuclear transplant embryos. The oocytes collected from slaughterhouse ovaries were matured for 24 h and then enucleated and cultured to allow cytoplasmic maturation and gain activation competence. And then the donor embryos were treated for 12 h with 10