• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.360-364
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• 2010

Food Culture business is a type of food to maintain variety and freshness of the material to individual retail business is doing. Speed for maintaining the freshness of ingredients, food sales and the cost of materials, labor, expense and lack of accuracy for the aggregate of the settlement issue is. In this paper the distribution of FC for the ERP project to build and aggregate revenue and cost elements of the business unit's sales against the cost of materials, labor, expense accounting, including aggregate elements, and also for purchase orders and bid off retail logistics estimates of the value of arms agreements with logistics Simulation results will be studied. If the attempt to close the business units and country units, and total assets of the unit to enable profit and loss calculations are to ERP research. Through this thesis FC business development and logistics ERP will contribute to the retail industry.

• Textile Coloration and Finishing
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• v.27 no.4
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• pp.229-244
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• 2015

The effects of synthesis conditions on characteristics of the calcium-azo complex pigment, C.I. Pigment Red 57:1, were studied. It was mainly considered that the industrially required synthesis conditions for lowering electrical conductivity of the pigment solution keeping pigment quality such as particle size and color characteristics. Three parameters were chosen as control factors during the synthesis. The first was the amount of hydrochloric acid added to transform sodium nitrite into nitrous acid. The second was the amount of calcium chloride added to insolubilize the synthesized azo dye. The final factor was pH control during the coupling reaction. The electrical conductivity and pigment aggregate particle size were dependent on the amount of hydrochloric acid and calcium chloride. Higher HCl concentration gave brighter yellowish-red color because of smaller particle aggregate size and narrower size distribution. Amount of charged ions in the synthesis process might affect the "lake" formation resulting different particle aggregate size and color shade.

• KSBB Journal
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• v.5 no.4
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• pp.347-353
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• 1990

The effects of nutrients and cell aggregate size on the cell growth of D. carota and the biosynthesi of carotenoid were investigated. Highest carotenoid content was obtained with sucrose as a carbon source and the equal ratio of ammonium to nitrate. High phosphate concentration stimulated the carotenoid biosynthesis in D. corota. 2,4-D inhibited the cell growth but stimulated the specific carotenoid content at high concentration. By modifying the medium composition based on these findings, three times higher specific carotenoid content and 2.5 times higher total carotenoid content were obtained as compared with the results obtained with basic MS media. Biosynthesis of carotenoid was found to be affected by cell aggregate size; high carotenoid production was obtained from the large aggregated cells resulted from high sucrose concentration.

• Proceedings of the Korea Society of Costume Conference
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• 2003.10a
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• pp.70-70
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• 2003

As the most closest everyday culture in connection with human lives, we can say that dress and its ornaments are the important elements in formation of human culture reflection political, economical, social and artistic reality as a cultural aggregate in that era.

• Plant Biotechnology Reports
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• v.2 no.1
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• pp.33-39
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• 2008

In this paper, we would like to show unexpected morphogenic potential of cell suspensions derived from seedling explants of Gentiana kurroo (Royle). Suspension cultures were established with the use of embryogenic callus derived from seedling explants (root, hypocotyl and cotyledons). Proembryogenic mass proliferated in liquid MS medium supplemented with $0.5mg\;l^{-1}$ 2,4-D and $1.0mg\;l^{-1}$ Kin. The highest growth coefficient was achieved for root derived cell suspensions. The microscopic analysis showed differences in aggregate structure depending on their size. To assess the embryogenic capability of the particular culture, 100 mg of cell aggregates was implanted on MS agar medium supplemented with Kin ($0.0-2.0mg\;l^{-1}$), $GA_3$ ($0.0-2.0mg\;l^{-1}$) and AS ($80.0mg\;l^{-1}$). The highest number of somatic embryos was obtained for cotyledon-derived cell suspension on $GA_3$-free medium, but the best morphological quality of embryos was observed in the presence of $0.5-1.0mg\;l^{-1}$ Kin, $0.5mg\;l^{-1}$ $GA_3$ and $80.0mg\;l^{-1}$ AS. The morphogenic competence of cultures also depended on the size of the aggregate fraction and was lower when size of aggregates decreased. Flow cytometry analysis reveled luck of uniformity of regenerants derived from hypocotyl suspension and 100% of uniformity for cotyledon suspension.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.40-46
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• 2007

This study was conducted to clarify the effects of the different substrates in terms of perlite (100%), peat moss (100%), granular rock wool (100%) and mixing perlite (50%) with peat moss (50%) on the growth responses and mineral contents of hydroponically grown Plantago asiatic in aggregate culture. Overall early plant growth such as plant height, stem diameter, number of leaves, root length, fresh and dry weight of shoot and root were increased in the order of that miked perlite and peat moss (50%:50%, v/v)>peat moss (100%)>granular rock wool (100%)>perlite (100%). Ca and Mg contents in plants became highest in the plants grown in the peat moss, however, Mg and Na in the granular rock wool. $P_2O_5$ content in plants were most increased in proportion to the plant growth increment in the peat moss (100%) and the mixing substrates of perlite and peat moss (50:50, v/v).

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.1
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• pp.30-38
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• 2008

Dental pulp cells are assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. The purpose of this study is to examine the effects of mineral trioxide aggregate (MTA) on various gene expression regarding dentinogenesis and cell viability assay in cultured primary human dental pulp cells. The author also examined the effects of this material on cellular alkaline phosphatase activity as a potential indicator of dentinogenesis. For gene expression on MTA, reverse transcriptase polymerase chain reaction was performed using primer sets of glyceraldehyde-3-phosphate dehydrogenase, type I collagen, alkaline phosphatase(ALP), osteonectin, and dentin sialoprotein after 2 and 4 days. Cell viability assay showed that the proportion of MTA-treated pulp cells which had been exposed for 5 days to MTA was higher than that of the control cells. Among the genes investigated in this study, ALP and osteonectin(SPARC) were increased in MTA treated group than in control. These findings suggest that this dental pulp culture system may be useful in the future as a model for studying the mechanisms underlying dentin regeneration after the treatment with MTA. Exposure to MTA material would not induce cytotoxic response in the dental pulp cells. In addition, MTA could influence the behavior of human pulp cells by increasing the ALP activity and SPARC synthesis.

• The Journal of Asian Finance, Economics and Business
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• v.5 no.3
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• pp.195-206
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• 2018

Paying close attention to those new to an organization, whether fresh or experienced, whose primary interest is in (re)socialization, the current study intends to (1) further the concept of mentoring from a bilateral relationship to a community and culture fostered by developmental networks, (2) propose an integrated conceptual framework for organizational socialization, and (3) suggest implications for practice and future research. This study reviews, analyzes, and integrates research assets and subsequently re-conceptualizes the aggregate information as valid propositions and a conceptual framework. The findings include (1) 11 propositions regarding the relationships among network characteristics (embeddedness, diversity), developmental functions (career support, psychosocial support, and role modeling), and socialization outcomes (learning and attitudinal outcomes); and (2) an integrated conceptual framework that depicts a comprehensive mechanism through which developmental networks conduce to organizational socialization of newcomers. Implications are that developmental networking must be an individual's fundamental competency and an essential part of organizational onboarding processes, and imperative for both members' career development and innovative organizational culture. By integrating research assets on the developmental phenomenon into conceptualizations, this study furthers the concept of mentoring to organizational culture and stimulates a substantive discourse for theory-building towards organizational socialization from the developmental network perspective.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.171-173
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• 2003

Suspension culture in serum-free medium is important for the efficient large-scale culture of anchorage-dependent cells that are utilized to produce therapeutic recombinant protein(e.g., insulin, antibody, vaccine) and virus vector for therapeutic gene transfer. We developed a novel method for the suspension culture of anchorage-dependent animal cells in serum-free medium using biodegradable polymer nanospheres in this study. Poly(lactic-co-glycolic acid) (PLGA) polymer nanospheres (433nm in average diameter) were used to the culture of human embryonic kidney 293 cells in serum-free medium in stirred suspension bioreactors. The use of PLGA nanospheres promoted the aggregate formation and cell growth (3.8-fold versus 1.8-fold growth), compared to culture without nanospheres. Adaptation of the anchorage-dependent cells to suspension culture or serum-free medium is time-consuming and costly. In contrast, the culture method developed in our study does not require the adaptation process. This method may be useful for the large-scale suspension culture of various types of anchorage-dependent animal cells in serum-free medium.