• The Korean Journal of Mycology
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• v.35 no.2
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• pp.101-108
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• 2007

Armillaria tabescens, one of edible and medicinal mushrooms belonging to Agaricales of Basidiomycota, has been known to have outstanding curative effects on chronic hepatitis and cholecystitis and inhibitory effects on the sarcoma 180 and Erhrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr, NaCl, Fr. HW and Fr, MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests showed that crude polysaccharides were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of $2000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of $28.8{\sim}46.5%$ in mice inoculated with Sarcoma 180, respectively. Fr. NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by $1.8{\sim}2.1$ folds, respectively. In case of Fr, NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 9 and 1.9 folds, respectively.

• The Korean Journal of Mycology
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• v.35 no.2
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• pp.109-114
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• 2007

Oudemansiella radicata, edible and medicinal mushroom belonging to Agaricales of Basidiomycota, has been known to exhibit outstanding curative effects on the fungal infection and hypertension caused by high blood pressure. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr, NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity test showed that Fr. NaCl was not cytotoxic against NIH3T3 and Sarcoma 180 at the concentration of $10{\sim}1,000\;{\mu}g/ml$. Intraperitoneal injection with Fr. NaCl exhibited antitumor activity with life prolongation effect of $42.9{\sim}66.7%$ in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by $1.4{\sim}3$ folds compared with controlled and LPS groups, respectively. Intraperitoneal injection with Fr. MeOH increased the numbers of peritoneal exudate cells and circulating leukocytes by 3.5 and 2.5 folds, respectively. Therefore, the antitumor effect exhibited on mouse Sarcoma 180 cells was likely due to immune-modulating activity of crude polysaccharides extracted from fruiting body of O. radicata.

• Journal of Korea Foresty Energy
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• v.21 no.3
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• pp.34-45
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• 2002

This study was carried out to investigate the diversity and frequency of ectomycorrhizal fungi by cutting levels in Quercus acuta and Pinus densiflora stands from June to October, 2001. The obtained results from investigation were as follows, 1. The total of fungi found during the surveyed periods were 50 species, 30 genus, 15 families. The fungi belonged to Agaricales(37 species, 18 genus, 7 families), Aphllophorales(7 species, 6 genus, 3 families), Auriculariales(1 species, 1 genus, 1 families) and Gasteromycetes(5 species, 5 genus, 4 families), Basidiomycota. 2. The total number of putative ectomycorrhizal fruiting bodies were 137 individuals, 22 species, 10 genus, 5 families, 79 individuals, 10 species, 6 genus, 3 families at strip-cutting area of Pinus densiflora stand, 24 individuals, 9 species, 5 genus, 4 families at strip-cutting area of Quercus acuta stand and 25 individuals, 10 species, 4 genus, 3 families at spacing cutting area of Quercus acuta stand. 3. Tylropilus neofelleus had high host specificity associated with Chamaecyparis obtusa, while Amanita pseudoporphyria had relatively wide host ranges compared to other ectomycorrhizal fungi. 4. The results analyzed by Correlation coefficients showed that relative humidity and precipitation were major enviromental factors to affect the distribution of ectomycorrhizal fruiting bodies.

• The Korean Journal of Mycology
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• v.22 no.1
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• pp.107-116
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• 1994

Interorder somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus in the order Agaricales and Elfvingia applanata in the order Aphyllophorales. The fusants were classified into stable heterokaryons and spontaneously segregated heterokaryons. Hyphae of all fusion products except two strains did not form clamp connections. Out of them, two clamped and three clampless fusants produced mature fruiting bodies by light-dark cycle on sawdust rice bran medium. All of these basidiocarps had clamp connections. Three fusants were analysed with the distribution of progenies and segregation of genetic characters by random spore analyses. The genetic markers were shown to segregate and recombine in the first generation of monospores isolated from basidiocarps. Phenotypes of a large number of auxotrophic progenies were not detected in the two clamped fusants. The aberration ratio of segregants indicated the gene interaction resulting from different genome structure between distantly related species. The polymerase chain reaction (PCR) was adopted for the detection of somatic hybrids nuclear DNA. Four fusants showed a positive results in three kinds of primers. The prominent reaction products are represented by new bands in primer # 87 and # 125. Out of four fusants, two somatic hybrids had non-parental mtDNA patterns when digested with EcoR1 and HindIII. Comparison of somatic hybrids, tissue culture isolates(TC) and multispore germination isolates(MS) were made using esterase isozyme analysis. It is apparent that somatic hybrids had a minor banding patterns which are quite different from those of parents.

• Journal of Mushroom
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• v.19 no.4
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• pp.279-284
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• 2021

Flammulina velutipes is the representative edible mushroom in Korea. It makes up around 18% of the total mushroom production and accounts for about 36% of the total mushroom export. Despite being one of the most industrially important mushroom in Korea, the distribution rate of domestic cultivar was low (~31.6%) in 2020. Therefore, we need a new domestic cultivar with high quality as the main export mushroom. We developed a new white domestic cultivar, 'Seolhan', bred by mating two monokaryotic strains isolated from Baekseung and Woori-1ho. The optimum mycelial growth temperature was 25℃ and Seolhan had higher mycelial growth than the control at 20 and 25℃. In bottle cultivation, the optimum temperature of incubation was from 15℃ to 17℃ and the growth temperature of Seolhan was 7-8℃, which was slightly higher than that of the control. The yield of Seolan was 226.5±8.0 g, an increase of 18.4% compared to the control. The period of primodia formation also was a couple of days ahead. In morphological traits, the pileus of Seolhan was smaller than that of the control, the stipe was thicker, and the color brighter. As the results of replacement culture and genetic diversity test, it was confirmed that Seolhan was genetically different from other white varieties.

• Journal of Mushroom
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• v.20 no.4
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• pp.208-217
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• 2022

Mushrooms in Naejangsan National Park between May and September of 2021 have been surveyed. In this period, a total of 4 divisions, 9 classes, 25 orders, 72 families, 171 genera, and 381 species, including 3 climate-sensitive biological indicator species were found. The order in which the most diverse array of species was observed is Agaricales, which includes 24 families, 64 genera, and 170 species. Among these, the genus Russula was dominant, with 30 species, followed by the genus Amanita with 27 species. Among the 12 grids we investigated, species diversity was greatest in grid F5, in which 56 species of mushrooms were found. In particular, a large number of ectomycorrhizal mushrooms, including Russula spp. and Lactarius spp. were recognized. We presume that the gentle slopes and the low occurrence of Sasa borealis in this area may create a favorable environment for wild mushrooms. In corroboration, some grids (e.g. F6, F8, and F10) covering steep slopes and harboring large numbers of Sasa borealis contained only 19 species. Based on DNA sequence analysis, the NJ21064 was identified as Chlorophyllum hortense, which is newly recorded in Korea.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.37-37
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• 2023

천마(天麻, Gastrodia elata Blume)는 난초과(蘭草科, Orchidaceae)에 속하는 식물로 잎과 뿌리가 없어 탄소동화능력이 없으며, 뽕나무버섯균과 공생하는 기생식물이다. 천마는 노지재배 시 혹한, 폭우 등 기상환경에 따른 연차간 수량성 차가 673~1,175kg/10a로 크고, 연작에 따른 수량성이 연작 1회 시 29%, 연작 2회 시 68%가 감소하는 경향을 보이고 있다. 최근 기후변화 대응으로 비가림시설재배가 이뤄지고 있으나, 비가림 시설재배 또한 연작장해가 발생하고 있다. 따라서, 본 연구는 비가림시설을 활용한 천마 재배의 연작장해 경감을 위해 태양열(6~10월, 비닐피복) 및 토양훈증(메탐소듐, 30 L/1,000 m²)으로 토양소독 처리를 하였고, 윤작(수단그라스)을 대조구로 설정하여 토양 화학성, 미생물상 및 수량성을 분석하였다. 토양소독 전·후 토양내 화학성을 분석한 결과, 비가림시설재배 시 토양 화학성의 변화는 거의 없었다. 토양소독 후 metagenome 분석 결과, JCR21(윤작), JCS21(태양열), JCF21(토양훈증)의 시료로부터 확보한 총 read는 598,425개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 2,397개(0.4%), no hit, not assign된 read는 17,094개 (2.9%), Bacteria로 분류된 read는 281,391개(47.0%), Eukaryota로 분류된 read는 총 297,543개(49.7%)였다. JCR21은 전체 read의 35.0%, JCS21은 34.0%, JCF21은 31.0%를 차지했고, Eukaryota는 JCR21 대비 JCS21, JCF21에서 각각 9.9%, 18.9% 낮았다. 그리고, Bacteria는 JCR21 대비 JCF21은 5.4% 감소하였으나, JCS21은 1.4% 증가하였다. 이 중 Eukaryota에서 종(species)명까지 정확하게 밝혀진 것은 27종이었고 속(genus) 으로는 18속이었다. JCF21은 전체 read의 30.2%, JCS21은 33.8%, JCR21은 36.0%를 차지했고, JCF21 포장은 토양훈증으로 JCS21에 비해 3.6%, JCR21에 비해 5.8%까지 균수가 감소함을 알 수 있었다. 대체적으로 발견된 균은 고르게 분포하고 있었으나 초작지, 연작 1회지, 연작 2회지에서는 많이 발견되지 않았던 Mucoromycota (41,490 read, 13.9%), Agaricales (10,586 read, 3.6%)가 높은 비율로 분포를 하고 있었다. 토양소독 처리에 따른 10a 당 수량을 살펴본 결과, 윤작 1,309 kg, 태양열소독 1,609 kg, 토양훈증 1,733 kg으로 나타났고, 수량지수가 윤작 처리구 대비 태양열소독은 23%, 토양훈증은 32% 높았다. 성마율은 윤작 51%, 태양열소독 63%, 토양훈증 68%으로 나타났으며, 자마율은 윤작 49%, 태양열소독 37%, 토양훈증 32%으로 나타났다. 괴경썩음 정도는 윤작 30-49%, 태양열소독 10-19%, 토양훈증 5-9%로 나타났다. 따라서, 비가림시설을 활용한 천마 재배 시 토양훈증 소독 처리를 하면 연작이 가능할 것으로 판단되었다.

• Journal of Mushroom
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• v.12 no.4
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• pp.330-340
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• 2014

Coprinellus micaceus, belongs to family Psathyrellaceae of Agaricales, Basidiomycota, has been used for edible purposes in the world. This study was initiated to evaluate the antioxidant, anti-diabetic, anti-cholinesterase, anti-tyrosinase, and nitric oxide inhibitory activities of fruiting bodies from C. micaceus extracted with methanol and hot water. The HPLC analysis of phenolic compounds from the mushroom extracts identified 4 phenolic compounds including procatechuic acid, chlorogenic acid, (-)-epicatechin, and naringin. In 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging assay, the scavenging activities of methanol and hot water extracts were lower than that of positive control, BHT. The chelating effects of methanol and hot water extracts were significantly higher than that of BHT, the positive control at the all concentrations tested. In the reducing power assay, methanol and hot water extracts exhibited the lower activities compared with positive control at the 0.125-0.2 mg/ml. The methanol and hot water extracts of the mushroom inhibited the ${\alpha}$-glucosidase activity by 62.26% and 67.59%, respectively at the 2.0 mg/ml, while acarbose, the positive control, inhibited the ${\alpha}$-glucosidase activity by 81.81% at the same concentration. In the acetylcholinesterase(AChE) inhibitory activity assay, methanol and hot water extracts of the mushroom inhibited the AChE by 94.64% and 74.19%, respectively at 1.0 mg/ml, whereas the galanthamine, standard drug, inhibited the AChE activity by 97.80% at the same concentration. The tyrosinase inhibitory activities of methanol and hot water extracts were 91.33% and 91.99% at 2.0 mg/ml, while the inhibitory activity of kojic acid, the positive control, was 99.61% at the same concentration. Nitric oxide(NO) production in lipopolysaccahride (LPS) activated RAW 264.7 cells were inhibited by the methanol and hot water extracts in a concentration dependent manner. Therefore, it is concluded that fruiting bodies of C. micaceus contained natural antioxidant, anti-acetylcholinesterase and ${\alpha}$-glucosidase inhibitory, anti-inflammatory, anti-tyrosinase substances which might be used for promoting human health.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.270-283
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• 2017

In a survey on higher fungi from 2004 to 2011, and also in 2013, in Naejangsan National Park, a total of 2 divisions, 7 classes, 21 orders, 74 families, 229 genera, and 521 species were observed. Dominant species belonged to the families Boletaceae and Russulaceae (44 species), Agaricaceae (35 species), Polyporaceae (29 species), and Amanitaceae (27 species). For the habitat environment, 21 families, 44 genera, and 192 species (36.9%) (63 species of poisonous mushroom, 79 species of edible and medicinal mushroom, and 43 species of unknown edible & poisonous mushroom) of ectomycorrhizal mushrooms were found; 41 families, 118 genera, and 199 species (38.2%) (14 species of poisonous mushroom, 85 species of edible & medicinal mushroom, and 90 species of unknown edible and poisonous mushroom) of litter decomposing and wood rotting fungi were found, and 29 families, 66 genera, and 121 species (23.2%) (8 species of poisonous mushroom, 54 species of edible and medicinal mushroom, and 47 species of unknown edible & poisonous mushroom) of grounding fungi were found, and 9 species were the other habitat. In terms of seasonality, most of the higher fungi were found in July, August, and September. In terms of altitude, the most species were observed at 200~299 m, and populations dropped by a significant level at an altitude of 700 m or higher. It seemed that the most diversified higher fungi occurred at climate conditions with a mean air temperature of $25.0{\sim}28.9^{\circ}C$, a maximum air temperature of $30.0{\sim}33.9^{\circ}C$, a minimum air temperature of $21.0{\sim}24.9^{\circ}C$, a relative humidity of 73.0~79.9%, and over 400.0 mm of rainfall.

• Journal of Mushroom
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• v.17 no.2
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• pp.52-63
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• 2019

Agrocybe spp. belongs to the Agaricales order, Bolbitiaceae family, and Agrocybe genus. In Korea, so far, it has been cultivated through bottle cultivation; therefore, this study was conducted for the development of a new cultivar using the bag cultivation method for quantitative improvement. Thirty-three gene resources of Agrocybe spp. were collected and their quantity and characteristics of bag cultivation were examined. Next, 5 kinds of crossing parents were selected based on the cultivation period and shape of the fruit body. Seven strains were selected through 3 cross combinations. The 7 selected strains and the comparative cultivar 'Cham' were cultivated in a bag. As a result, the cultivation period was 49 days for 'JBAC15-1' and 50 days for 'JBAC15-6' which are 4 days and 3 days less than the cultivation period of the comparative cultivar 'Cham'(53 days), respectively. Cultivation periods of other strains except for 'JBAC15-1' and 'JBAC15-1' were longer than that of the comparative cultivar'Cham'. The best ratio of primordia formation among the selected strains was found to be that of 'JBAC15-1' with 96.1% followed by 'JBAC15-6' with 94.5%. These rates were 3.1% and 1.5% higher than the ratio of primordia formation of the comparative cultivar 'Cham', which is found to be 93.0%. The quantity was maximum in the 'JBAC15-1' cultivar with 176.8 g per bag followed by 'JBAC15-6' with 168.7 g per bag. The quantities were 10% and 5% more than the comparative cultivar 'Cham' with 160.7 g per bag. Based on these results, 'JBAC15-1' and 'JBAC15-6', which had shorter cultivation periods and more quantities than the comparative cultivar 'Cham' were finally selected. For the selected strains of 'JBAC15-1' and 'JBAC15-6', mycelial growth was observed to be optimal on PDA medium and the optimum temperature was $27.5^{\circ}C$. The optimum pH was pH 5 for 'JBAC15-1' and pH 6 for 'JBAC15-6'. The color of the pileus of the fruit body was dark brown in 'JBAC15-1' and 'Cham' and light brown in 'JBAC15-6'. The pileus was hemispherical in shape in both 'JBAC15-1' and 'Cham'. However, the colors of the stem were different - light brown in 'JBAC15-1', white in 'JBAC15-6', and ivory in the comparative cultivar 'Cham'.