• Journal of Microbiology
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• v.34 no.2
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• pp.117-123
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• 1996

Restriction-polymorphic patterns of nuclear-ITS were examined for the genetic relationships among 12 bisidiomycetous mushrooms to Aphyllophorales and Agaricales. The taxonomic affinity of Ganoderma species with the family Polyporaceae also was examined. With 13 restriction endonucleases, 159 restriction characters were generated form the 12 species examined. UPGMA and neighbor-joining analyses separated the 12 species into two genetically distinct groups that correspond to orders (Agaricales and Aphyllophorales) where each species is included. This result indicates that there is clear genetic demarcation between Agaricales and Aphyllophorales. Dendrograms constructed by several data analyses showed that even though Ganoderma species are somewhat in intermediate taxonomic position between the Polyporaceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

• Mycobiology
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• v.50 no.4
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• pp.219-230
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• 2022

Agaricales species form pileate-stipitate fruiting bodies and play important roles in maintaining the terrestrial ecosystem as decomposers, symbionts, and pathogens. Approximately 23,000 Agaricales species have been known worldwide, and 937 species have been recorded in the Republic of Korea. However, most of them were identified solely based on morphological characteristics that often led to misidentifications. The specimens collected from 2018 to 2020 in the Republic of Korea were identified based on phylogenetic analysis of the internal transcribed spacer (ITS) sequences. Their identities were confirmed by microscopic characteristics. As a result, 14 Agaricales species were discovered for the first time in the Republic of Korea. They belonged to nine genera: Agaricus, Calocybe, Cortinarius, Hygrocybe, Inocybe, Lepista, Leucoagaricus, Marasmius, and Psathyrella. Detailed macroscopic and microscopic descriptions were provided to help distinguish these species. The morphological and molecular data provided in this study will serve as reliable references for the identification of Agaricales species.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.281-289
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• 2019

Since 2013, more than 100 Entoloma (Entolomataceae, Agaricales) collections have been made during field investigations of mushroom flora in Korea. Among these collections, three Entoloma species were identified as new records from Korea. Morphological examinations were made based on the macro- and micro-morphological features of the isolates collected during field visits. To ensure the identity of the isolates at the species level, DNA sequences from four gene regions (rpb2, ITS, 28S, and mtSSU) were compared. To the best of our knowledge, these are the first records of E. aprile, E. chytrophilum, and E. hirtipes in Korea. Comprehensive descriptions, photographs, and phylogenetic examinations are presented here.

• Current Research on Agriculture and Life Sciences
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• v.22
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• pp.29-39
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• 2004

This study was conducted to provide a fundamental information for commercial, medical usage and mushroom gene preservation and to investigate the diversity of indigenous mushroom on Bonghwa-Gun. A total of 3 classes 2 subclasses 13 orders 45 families 64 genera and 118 species including saprophytic and ectomycorrhizal fungi was investigated. The mushroooms are classified into 17 families 32 genera and 80 species in Agaricales, 13 families 17 genera and 22 species in Aphllophorales, 2 families 2 genera and 2 species in Heterobasidiomycetes, 9 families 9 genera and 10 species in Gasteromycetidae. There were edible mushroom of 46 species at Moonsu and Chungryang Mountain. The mushroom occurrence was closely related to vegetation and soil environmental conditions.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.139-153
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• 2021

The Inocybaceae is a family of ectomycorrhizal fungi belonging to the Agaricales order. Most species in this family produce secondary metabolites, such as muscarine, psilocybin, and other compounds. In the present study, we conducted taxonomic studies on the Inocybaceae based on morphological and molecular phylogenetic analyses for the first time in Korea. As a result, seven species of Inocybaceae were newly recorded: Inocybe caroticolor, I. fuscidula, I. grammatoides, I. latibulosa, I. stellata, Inosperma shawarense, and Mallocybe malenconii. Among them, the genera Inosperma and Mallocybe were described for the first time in Korea. Relevant morphological characteristics, phylogenetic trees, and photographs are provided.

• Mycobiology
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• v.48 no.4
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• pp.288-295
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• 2020

Species of Laccaria (Hydnangiaceae, Agaricales, and Basidiomycota) are well-known ectomycorrhizal symbionts of a broad range of hosts. Laccaria species are characterized by brown, orange, or purple colored basidiocarps, and globose or oblong, echinulate and multinucleate basidiospores. While some Laccaria species are easily identified at the species level using only the morphological characteristics, others are hard to distinguish at the species level due to small differences in morphology. Heretofore, ten Laccaria species have been reported in Korea. While studying the fungal diversity in the National Parks of Korea, two new Laccaria species were discovered. Species identification was done based on molecular analyses (ITS, 28S rDNA, rpb2, and tef1), then were confirmed by their corresponding morphologies. The two newly discovered Laccaria species are proposed here as Laccaria macrobasidia and Laccaria griseolilacina. The unique morphological characters of L. macrobasidia that distinguish it from its closely related species are orange-brown colored basidiocarp, long basidia and the absence of cheilocystidia. L. griseolilacina is characterized by a light grayish lavender-colored pileus and the absence of cheilocystidia. Two new species are described and illustrated in the present paper.

• Mycobiology
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• v.48 no.1
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• pp.58-69
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• 2020

Meghalaya, (in India), in the region of the mega-biodiversity hotspots, is home to a plethora of wild mushrooms. The present study concerns the exploration of the order Agaricales, which includes rare gilled mushrooms considered endangered under IUCN A4c criteria, due to the declining habitat. Electron microscopy of the gill sections revealed an abundance of clamp connections, hyphal cell walls, cystidia, and basidia. This rare species which belongs to the family Cyphellaceae, exhibits morphological and molecular differences from the Cyphella spp. Phylogenetic analysis revealed that it formed a clade under the genus Campanophyllum of the order Agaricales, confirmed by both Neighbor Joining (NJ) and Bayesian phylogenetic analysis. Being nutritionally potent along with its efficient antioxidant value, the fungal extract shows significant rise of two-fold in the antimicrobial activity along with the commercial antibiotics. The compound, Phenol, 2, 4-bis (1, 1-Dimethylethyl) (2, 4-DTBP) showed in ample range in the fungal extract along with aliphatic hydrocarbons, terpene, alcohol and volatile organic compounds on further characterization in GCMS. The present study indicates the endangered Campanophyllum proboscideum could be a rich source of natural antioxidants and an effective pharmaceutical agent.

• Mycobiology
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• v.43 no.3
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• pp.239-257
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• 2015

We conducted five times surveys, in June, September and October in 2012; June and September 2013, to catalog the mushroom flora in Ulleung-gun, Republic of Korea. More than 400 specimens were collected, and 317 of the specimens were successfully sequenced using the ribosomal DNA internal transcribed spacer barcode marker. We also surveyed the morphological characteristics of the sequenced specimens. The specimens were classified into 2 phyla, 7 classes, 21 orders, 59 families, 122 genera, and 221 species, and were deposited in the herbarium of Korea National Arboretum. Among the collected species, 72% were saprophytic, 25% were symbiotic, and 3% were parasitic. The most common order was Agaricales (189 specimens, 132 species), followed by Polyporales (47 specimens, 27 species), Russulales (31 specimens, 22 species), Boletales (10 specimens, 7 species), and so on. Herein, we also reported the first Bovista species in Korea, which was collected from Dokdo, the far-eastern island of Korea.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.192-196
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• 2010

Two species of the Korean Agaricales are described and illustrated in this paper. Two genera Cystoagaricus and Limacella are new to Korean agaric flora. All of these, Cystoagaricus strobilomyces and Limacella glioderma are recorded for the first time in the Korean fungus flora. The collections cited are all preserved in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.220-232
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• 2016

A survey of ectomycorrhizal fungi was performed during 2009-2011 and 2015 in Byeonsanbando National Park. A total of 3,624 individuals were collected, which belonged one division, 1 class, 5 orders, 13 families, 33 genera, 131 species. The majority of the fruiting bodies belonged to orders Agaricales, Russulales, and Boletales, whereas a minority belonged to orders Cantharellales and Thelephorale. In Agaricales, there were 6 families, 9 genera, 49 species, and 1,343 individuals; in Russulales, 1 family, 2 genera, 35 species, and 854 individuals; in Boletales, 4 families, 19 genera, 40 species, and 805 individuals; in Cantharellales, 1 family, 2 genera, 5 species, and 609 individuals; and in Thelephorale, 1 family, 1 genus, 2 species, and 13 individuals. The most frequently observed families were Russulaceae (854 individuals representing 35 species), Boletaceae (652 individuals representing 34 species), and Amanitaceae (754 individuals representing 25 species). The greatest numbers of overall and dominant species and individual fruiting bodies were observed in July. Most species and individuals were observed at altitudes of 1~99 m, and population sizes dropped significantly at altitudes of 300 m and higher. Apparently, the highest diversity of species and individuals occurred at climatic conditions with a mean temperature of $23.0{\sim}25.9^{\circ}C$, maximum temperature of $28.0{\sim}29.9^{\circ}C$, minimum temperature of $21.0{\sim}22.9^{\circ}C$, relative humidity of 77.0~79.9%, and rainfall of 300 mm or more.