• Title/Summary/Keyword: action-spectrum

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Action Spectra of Apoptosis Induction and Reproductive Cell Death in L5178Y cells in UV-B Region

  • Mizuho Aoki;Yoshiya Furusawa;Higashi, Sho-ichi;Masakatsu Watanabe
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.454-456
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    • 2002
  • It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

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ON ACTION SPECTRUM BUNDLE

  • Cho, Yong-Seung;Yoon, Jin-Yue
    • Bulletin of the Korean Mathematical Society
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    • v.38 no.4
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    • pp.741-751
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    • 2001
  • In this paper when $(M, \omega)$ is a compact weakly exact symplectic manifold with nonempty boundary satisfying $c_1|{\pi}_2(M)$ = 0, we construct an action spectrum bundle over the group of Hamil-tonian diffeomorphisms of the manifold M generated by the time-dependent Hamiltonian vector fields, whose fibre is nowhere dense and invariant under symplectic conjugation.

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NORMALIZATION OF THE HAMILTONIAN AND THE ACTION SPECTRUM

  • OH YONG-GEUN
    • Journal of the Korean Mathematical Society
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    • v.42 no.1
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    • pp.65-83
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    • 2005
  • In this paper, we prove that the two well-known natural normalizations of Hamiltonian functions on the symplectic manifold ($M,\;{\omega}$) canonically relate the action spectra of different normalized Hamiltonians on arbitrary symplectic manifolds ($M,\;{\omega}$). The natural classes of normalized Hamiltonians consist of those whose mean value is zero for the closed manifold, and those which are compactly supported in IntM for the open manifold. We also study the effect of the action spectrum under the ${\pi}_1$ of Hamiltonian diffeomorphism group. This forms a foundational basis for our study of spectral invariants of the Hamiltonian diffeomorphism in [8].

Action Spectra for Light-Induced De-Epoxidation and Epoxidation of Xanthophylls in Spinach Leaf

  • Lee, Kang-Ho
    • Applied Biological Chemistry
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    • v.9
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    • pp.1-7
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    • 1968
  • The action Spectra for violaxanthin de-epoxidation and zeaxanthin expoxidation in New Zealand spinach leaf segments Tetragonia expansa, were determined at equal incident quanta of $2.0{\times}10^{15}$ quanta $cm^{-2}$ $second^{-1}$. The action spectrum for de-epoxidation had major peaks at approximately 180 and 648 nm. Blue light was slightly more effective than red light and little activity was observed beyond 700 nm. The action spectrum for epoxidation showed major peaks at around 441 and 670 nm. Blue light was more effective than red light and light beyond 700 nm showed definite activity. The net result of de-epoxidation and epoxidation is a cyclic scheme, the violaxanthin cycle, which consumes $O_2$ and photoproducts. The action spectra indicate that the violaxanthin cycle is more active m clue than in red light and therefore could accout for $O_2$ uptake stimulated by blue light. The differences between the action spectra for de-epoxidation suggest that possibly two photosynthetic systems are involved. It was suggested that the violaxanthin cycle may functional a pathway for the consumption of excess photoproducts generated in blue light or the conversion of these photoproducts to other forms of energy.

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Photokinesis of Cyanobacterium Synechocystis sp. PCC 6803

  • Chung, Young-Ho;Park, Young-Mok;Moon, Yoon-Jung;Lee, Eun-Mi;Choi, Jong-Soon
    • Journal of Photoscience
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    • v.11 no.3
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    • pp.89-94
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    • 2004
  • Motile cyanobacterium Synechocystis sp. PCC 6803 cells show photomovement with respect to the light stimulus. Under lateral irradiation, Synechocystis displays a phototactic gliding movement toward the light source by a twodimensional random biased walk. Under vertical irradiation, Synechocystis decreased the frequency of mean vectorial gliding speed dependent on the applied fluence rate, whereas the deviation distribution width of the speed increased. This strongly suggests the involvement of photokinesis. Evidence for the cyanobacterial photokinesis was discussed in the previous report (Choi et al., 1999. Photochem. Photobiol. 70, 95-102) demonstrating that the gross scalar speed of vertically irradiating cells increased by about 50% compared with that of dark-adapted cells. In the visible wavelength range, Synechocystis cells showed a maximal photokinetic activity at 420 nm and a second maximal activity at 680 nm. The threshold action spectrum for the photokinesis resembles the absorption spectrum of chlorophyll with major differences in the phototaxis action spectrum at 560 nm and 660 nm. We postulate that the cyanobacterial photokinesis is powered by the energy-generating chlorophyll pigments.

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An Action Spectrum for Light-induced Growth rate in Pleurotus ostreatus (느타리버섯 생장에 미치는 광 감응성 작용 스펙트럼)

  • 이갑득
    • Journal of Life Science
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    • v.10 no.5
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    • pp.519-523
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    • 2000
  • The action spectrum for light-induced growth of fruit body in Pleurotus ostreatus has been studied by irradiation at various wavelengths. Effective wavelengths were distributed from near ultraviolet to blue region of spectrum. The most effect of light was observed in the region between 340 to 520 nanometers. The growth weigth obtained from which has been irradiated 144 hours in the region of 340-520 nm increases 74%, on the contrary the observed growth weight has been observed in the longer wavelengths than 620 nm. The pileus size was promoted 30$\times$32% in the region of 340-500 nm, and retarded 22$\times$19% in the dark. the strip length was rearded 12% in the region of 340-500 nm, and promoted 38% in the dark. The color thickness for pileus is getting light in longer wavelengths.

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An Action Spectrum for Light-induced Mycelial Growth and Primordium Formation in Pleurotus ostreatus (느타리버섯의 균사체 및 원기 형성에 미치는 광 감응성 작용 스펙트럼)

  • 이갑득;강병수;박용기
    • Journal of Life Science
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    • v.6 no.3
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    • pp.193-197
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    • 1996
  • The action spectrum for mycelial growth and fruitbody primordium formation in Pleurotus ostreatus has been studied by irradiation at various wavelengths. Effective wavelengths. Effective wavelengths were distributed from near ultraviolet to blue region of spectrum. The most effect of light was observed in the region between 340 to 500 nanometers. The light intensity required to obtain of the maximum effect at the most effective wavelengths(430-500nm), was over 6.8 mW/Cm$^{2}$. Up to 6.8 mW/Cm$^{2}$, the primordium formed about 25 hr after the start of illumination. The higher the light intensity, the earlier the formation of the primordium formation : Up to about 6.8 mW/Cm$^{2}$.

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Response of Skew Bridges with permutations of geometric parameters and bearings articulation

  • Fakhry, Mina F.;ElSayed, Mostafa M.;Mehanny, Sameh S.F.
    • Earthquakes and Structures
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    • v.17 no.5
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    • pp.477-487
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    • 2019
  • Understanding the behavior of skew bridges under the action of earthquakes is quite challenging due to the combined transverse and longitudinal responses even under unidirectional hit. The main goal of this research is to assess the response of skew bridges when subjected to longitudinal and transversal earthquake loading. The effect of skew on the response considering two- and three- span bridges with skew angles varying from 0 to 60 degrees is illustrated. Various pier fixities (and hence stiffness) and cross-section shapes, as well as different abutment's bearing articulations, are also studied. Finite-element models are established for modal and seismic analyses. Around 900 models are analyzed under the action of the code design response spectrum. $Vis-{\grave{a}}-vis$ modal properties, the higher the skew angle, the less the fundamental period. In addition, it is found that bridges with skew angles less than 30 degrees can be treated as straight bridges for the purpose of calculating modal mass participation factors. Other monitored results are bearings' reactions at abutments, shear and torsion demand in piers, as well as deck longitudinal displacement. Unlike straight bridges, it has been typically noted that skew bridges experience non-negligible torsion and bi-directional pier base shears. In a complementary effort to assess the accuracy of the conducted response spectrum analysis, a series of time-history analyses are applied under seven actual earthquake records scaled to match the code design response spectrum and critical comparisons are performed.

Arching Action Effect for Inelastic Seismic Responses of Bridge Structures (교량의 비탄성 지진응답에 대한 아칭작용의 영향)

  • Song, Jong-Keol;Nam, Wang-Hyun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.2A
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    • pp.131-143
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    • 2009
  • Under transverse earthquake shaking, arching action of bridge structures develops along the deck between the abutments thus providing the so-called deck resistance. The magnitude of the arching action for bridge structures is dependent on the number of spans, connection condition between deck and abutment or piers, and stiffness ratio between superstructure and substructure. In order to investigate the arching action effects for inelastic seismic responses of PSC Box bridges, seismic responses evaluated by pushover analysis, capacity spectrum analysis and nonlinear time-history analysis are compared for 18 example bridge structures with two types of span numbers (short bridge, SB and long bridge, LB), three types of pier height arrangement (regular, semi-regular and irregular) and three types of connection condition between superstructure and substructure (Type A, B, C). The arching action effects (reducing inelastic displacement and increasing resistance capacity) for short bridge (SB) is more significant than those for long bridge (LB). Semi-regular and irregular bridge structures have more significant arching action than regular bridges.

Influence of the Random Yield Strength Distribution on the Behaviour Factor of Steel Structures (임의항복강도의 분포가 강구조물의 거동계수에 미치는 영향)

  • Kook, Seung Kyu
    • Journal of Korean Society of Steel Construction
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    • v.9 no.2 s.31
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    • pp.229-235
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    • 1997
  • In order to check the influence of the randomness in yield strengths on the energy dissipation capacity of steel structures, behaviour factors applied for the "Response Spectrum Method" and their distributions are determined in this study with 7 steel framed models. Also 4 artificial accelerograms simulated with a given spectrum are applied to check the influence of the randomness in seismic action on the behviour factor. To execute numerous time-step calculations for the investigation a time-step analysis method is developed and applied after the reliability estimation to determine the action effects.

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