• 현장농수산연구지
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• 제5권1호
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• pp.20-27
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• 2003

유색칼라 조직배양묘의 순화재배 용토선발실험에서 초기생육 출엽율은 큰 차이가 없었으나 생육 6개월 후의 괴경비대는 모래(50%)+Peatmoss(50%) 처리에서 가장 좋았다. 순화재배 시기별 순화율은 8월 20일 정식에서 64%~70.4%로 가장 저조하였고, 3월 20과 4월 20일은 90%이상의 순화율을 나타냈다. 괴경비대은 3월 20일 정식에서 구중이 가장 무거웠다. 순화 재배시 배양묘의 재식거리 10×10cm에서 괴경중이 가장 양호했다.

• 한국작물학회지
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• 제47권3호
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• pp.186-188
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• 2002

생물반응기에서 배양 수확한 신초의 발근과 순화조건을 알기 위하여 실험한 결과는 다음과 같다. 배양배지는 MS 기본배지의 농도를 반으로 줄이고 아가 농도를 1.2%로 하는 것이 신초의 발근과 순화에 유리하였다. 여과막이 부착된 배양병에서 신초를 생육시키는 것이 밀폐된 배양병에서 생육시킨 것보다 생육이 양호하고 생존율도 높았다. 배지에 paclobutrazol을 0.3-0.4mg/l로 처리한 경우 신초의 발근과 생육이 양호하였다.

• 식물조직배양학회지
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• 제25권5호
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• pp.315-318
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• 1998

약배양 유래 녹색꽃양배추의 유식물 순화율 및 배수성을 조사하였다. 7가지 공시 품종중 순화율에 있어 Marathon은 80.8∼100%, Landmark는 79.2∼100%, Mariner는 71.4∼79.2%로 나타나 평균 순화율은 83.6%∼86.1% 이였다. 소포자 배에서 생성된 식물체의 배수성은 공변세포내 엽연체수 측정으로도 가능했으며, 또한 배수성의 조기측정을 위해 I-KI용액을 사용하여 엽록체의 즉시염색 관찰이 가능했다. 관찰된 평균 엽녹체수는 반수체가 8.5, 2배체는 13.5, 그리고 4배체는 18.5개로 나타 났으며 이를 기초로 분류한 재분화개체의 배수성은 반수체 47.1∼51.3%, 2배체 47.9∼51.7%, 4배체 0.8∼l.2% 로서 반수체와 2배체가 각각 반씩이고 4배체가 극히 적게 분포하며 인위적인 염색체 배가 없이도 상당수의 자연 배가된 2배체를 얻을 수 있었다.

• 한국작물학회지
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• 제62권1호
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• pp.60-65
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• 2017

이 시험은 '솔라라' 품종의 종자생산을 위해 분무경 수경재배에 적합한 묘를 구명하고자 2015년 3월 16일 배양순화묘 등 8처리의 묘를 분무경 베드에 정식하여 정식 후 70, 78일에 각각 생육과 수량특성을 조사하였다. 경장은 경삽 15일묘와 배양순화묘가 작은 편이었고 경삽묘의 육묘일수가 늘어남에 따라 길어지는 경향이었다. 생체중도 경삽 40일묘가 주당 122 g으로 가장 무거웠고 무발근묘와 배양순화묘는 가장 가벼웠다. 1차 복지는 경삽 35일묘에서 주당 12.9개로 가장 많았고 2차 복지는 경삽 35일묘, 배양순화묘, 경삽 30일묘에서 많이 발생하였다. 10주당 총괴경수는 배양순화묘가 145개로 많았으나 3~50 g(상품) 괴경수는 경삽 35일묘가 108개로 가장 많았고, 괴경 무게는 경삽 35일묘가 1,947 g으로 가장 무거웠고 다음으로 경삽 30일묘였다. '솔라라' 품종 수경재배 시에는 경삽한 후 30일~35일(경장 15 cm, 마디 12절) 묘로 정식하는 것이 수량성을 높일 수 있었다.

• ALGAE
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• 제30권2호
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• pp.103-119
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• 2015

Photosynthetic rates of the large centric diatom Coscinodiscus granii were measured by means of multicolor variable chlorophyll fluorescence imaging, single cell $^{14}C$ assimilation, and optical $O_2$ sensor measurements during light acclimatization of cultures grown at five different irradiances: 50, 150, 235, 332, and $450{\mu}mol$ photons $m^{-2}\;s^{-1}$. Photo-acclimatization was evident from changes of cellular chlorophyll a content, growth rates, and light response curves. Each of the applied methods evaluates different parts and reactions in the photosynthetic apparatus, which makes a direct quantitative comparison of rates difficult, although a different degree of correlation were found between all three methods. However, when used in combination, they provide information about the internal relationship of photosynthetic pathways as well as the variation in photosynthetic capacity between individual cells within a single algal culture.

• 한국자원식물학회지
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• 제36권6호
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• pp.588-596
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• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• Journal of Plant Biotechnology
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• 제44권4호
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• pp.431-437
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• 2017

Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar 'Cheongsu' which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.104-109
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• 2016

Rosa rugosa is a medicinal, ornamental, and edible plant native to Eastern Asian countries, including Korea, Japan, and China. The aim of this study was to establish a system for biomass production and secondary metabolite accumulation during in vitro culture and acclimatization of Rosa rugosa. The highest rate of multiple shoot proliferation was achieved with $8.8{\mu}M$ benzyladenine (BA) (83.3%). However, the number of shoots (14.4 per explant) at $4.4{\mu}M$ BA was higher than that at $8.8{\mu}M$ BA. Compared to BA, a combination of thidiazuron (TDZ) and indole butyric acid (IBA) exhibited significantly lower shoot induction, with only 50.0~79.2% and 4.2~16.7% relative shoot formation, respectively. During acclimatization, shoots were sampled every week and their total phenolic contents were analyzed. Among various growth factors, fresh weight showed the most dramatic increase from the 3rd week (88.0 mg/plant) to 4th week (132.7 mg/plant). Total phenolics and flavonoids contents were the highest at $1^{st}$ week of acclimatization. Depending on developmental stages, total phenolics and flavonoids contents were higher in 1-yr-old shoots grown ex vitro than in those of older field-grown or in vitro-grown plants. Amongst different ages of field grown plants, 6-year-old plants, the oldest in this study, showed the lowest content in total phenolics.

• Journal of Biosystems Engineering
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• 제32권2호
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• pp.109-114
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• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

• Journal of Ecology and Environment
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• 제46권3호
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• pp.218-242
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• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.