KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Rooting and Acclimatization of Shoots Harvested from Bioreactor Culture in Rehmania glutinosa

생체반응기에서 수확한 지황 신초의 발근과 순화

  • Koh, Eun-Jung (School of Plant Science, Seoul National University) ;
  • Chae, Young-Am (School of Plant Science, Seoul National University)
  • 고은정 (서울대학교 식물생산과학부) ;
  • 채영암 (서울대학교 식물생산과학부)
  • Published : 2002.06.01
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Abstract

This experiment was carried out to know the effect of media and agar concentrations, aeration and growth regulators on rooting and acclimatization of the shoots harvested from bioreactor culture in Rehmannia glutinosa. Half MS media with 1.2% agar improved rooting and acclimatization of shoots. Shoots were effectively acclimatized and rooted well in case of aeration by using membrane filtered vessels. Shoots acclimatized in vessel with membrane Inter were healthier and had higher ex vitro survival rate than those without membrane Inter on plug tray. Addition of paclobutrazol 0.3-0.4 mg/L, to acclimatization media enhanced shoots growth and root development.

생물반응기에서 배양 수확한 신초의 발근과 순화조건을 알기 위하여 실험한 결과는 다음과 같다. 배양배지는 MS 기본배지의 농도를 반으로 줄이고 아가 농도를 1.2%로 하는 것이 신초의 발근과 순화에 유리하였다. 여과막이 부착된 배양병에서 신초를 생육시키는 것이 밀폐된 배양병에서 생육시킨 것보다 생육이 양호하고 생존율도 높았다. 배지에 paclobutrazol을 0.3-0.4mg/l로 처리한 경우 신초의 발근과 생육이 양호하였다.

Keywords

References

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