• Bulletin of the Korean Chemical Society
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• v.31 no.12
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• pp.3771-3776
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• 2010

The dynamics of the $CN^-$-ligated ferric cytochrome c (CytcCN) in $D_2O$ at 283 K following Q-band photoexcitation at 575 nm was observed using femtosecond time-resolved vibrational spectroscopy. The equilibrium vibrational spectrum of the CN stretching mode of CytcCN shows two overlapping bands: one main band (82%) at $2122\;cm^{-1}$ with $23\;cm^{-1}$ full width at half maximum (fwhm) and the other band (18%) at $2116\;cm^{-1}$ with $7\;cm^{-1}$ fwhm. The time-resolved spectra show bleaching of the CN fundamental mode of CytcCN and two absorption features at lower energies. The bleach signal and both absorption features are all formed within the time resolution of the experiment (< 200 fs) and decay with a life time of 1.9 ps. One transient absorption feature, appearing immediately red to the bleach signal, results from the thermal excitation of low-frequency modes of the heme that anharmonically couple to the CN fundamental mode, thereby shifting the CN mode to lower energies. The shift of the CN mode decays with a lifetime of 2 ps, equivalent to the time scale for vibrational cooling of the low-frequency heme modes. The other transient absorption feature, which is 3.3 times weaker than the bleach signal and shifted $27\;cm^{-1}$ toward lower energies, is attributed to the CN mode in an electronically excited state where the CN bond is weakened with a lowered extinction coefficient. These observations suggest that photoexcited CytcCN mainly undergoes ultrafast radiationless relaxation, causing photo-deligation of $CN^-$ from CytcCN highly inefficient. As also observed in $CN^-$-ligated myoglobin, inefficient ligand photodissociation might be a general property of $CN^-$-ligated ferric hemes.

• Journal of The Korean Astronomical Society
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• v.54 no.5
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• pp.139-155
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• 2021

We present an updated version of the multilayer spectral inversion (MLSI) recently proposed as a technique to infer the physical parameters of plasmas in the solar chromosphere from a strong absorption line. In the original MLSI, the absorption profile was constant over each layer of the chromosphere, whereas the source function was allowed to vary with optical depth. In our updated MLSI, the absorption profile is allowed to vary with optical depth in each layer and kept continuous at the interface of two adjacent layers. We also propose a new set of physical requirements for the parameters useful in the constrained model fitting. We apply this updated MLSI to two sets of Hα and Ca II line spectral data taken by the Fast Imaging Solar Spectrograph (FISS) from a quiet region and an active region, respectively. We find that the new version of the MLSI satisfactorily fits most of the observed line profiles of various features, including a network feature, an internetwork feature, a mottle feature in a quiet region, and a plage feature, a superpenumbral fibril, an umbral feature, and a fast downflow feature in an active region. The MLSI can also yield physically reasonable estimates of hydrogen temperature and nonthermal speed as well as Doppler velocities at different atmospheric levels. We conclude that the MLSI is a very useful tool to analyze the Hα line and the Ca II 8542 line spectral daya, and will promote the investigation of physical processes occurring in the solar photosphere and chromosphere.

• Nuclear Engineering and Technology
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• v.56 no.10
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• pp.4097-4102
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• 2024

A dosimetry system's ability to monitor absorbed doses is crucial for the quality assurance and control of products that have been exposed to radiation. Here, we propose a polyvinyl chloride (PVC)-methyl yellow (MY) film as a potential dosimeter for irradiation facilities. The PVC-MY film was prepared with a simple solvent-casting method at different MY concentrations from 0.3 to 1.2 mM. To evaluate the dosimeter's performance, the film was exposed to gamma-irradiation with irradiation doses of 0-25 kGy. The optical characteristics of the films examined using a UV-Vis spectrophotometer showed that the color of the films changed from yellow to red with increasing irradiation doses, and the color change sensitivity of the films increased with increasing MY concentrations. The absorption spectra of the irradiated PVC-MY films showed three maximum absorption peaks at 417, 522, and 547 nm. As irradiation doses increased, the film absorption at 417 nm decreased, while the film absorption at 522 and 547 nm increased. The film's stability studies suggest storage of the irradiated film in a cold-dark conditions is recommended to prevent damage and maintain response stability. The findings in this study emphasize the potential of the PVC-MY film as a dosimeter for irradiation facilities.

• Journal of the Korean Applied Science and Technology
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• v.36 no.1
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• pp.355-361
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• 2019

The red pigment extracted from Serratia marcescens 2354 (ATCC 25419) was prodigiosin (PG), which was dissolved in methanol and measured for ultraviolet and visible light absorption spectra. It was the typical absorption spectrum of PG in an acid solution with ${\lambda}_{max}=537nm$. When the concentration of PG was increased from $1.0{\times}10-5M$ to $9.0{\times}10-5M$ in the methanol solution, the absorption intensity at 537 nm was increased, the absorption intensity at 467 nm was decreased, and the isosbestic point at 500 nm was observed. This phenomenon can be regarded as a result of reversible acid-base equilibrium reaction considering 537 nm and 467 nm of PG absorption band in acid and base solution respectively and isosbestic point of 500 nm. On the other hand, when the concentration of PG was reduced from $6.0{\times}10-4$ to $1.0{\times}10-4M$ in acetic acid buffer solution at pH 4.75, a new absorption band with ${\lambda}$ max at 500 nm appeared. This absorption band appears only in the aqueous solution of pH 4.75 and does not appear in the pure methanol solution of the same pH. This is due to the conversion of the PG molecule from the ${\alpha}$-isomer to the ${\beta}$-isomer by $H_2O$. In other words, it was confirmed that the color change of the PG can be caused by the concentration of the solution and the characteristics of the solvent.

• Korean Journal of Mineralogy and Petrology
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• v.36 no.4
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• pp.259-271
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• 2023

Since the short-wave infrared spectrum has a significant range of variation depending on the production environment, countries with advanced resource exploration are collecting the spectrum and building a database. Representative organizations include the USGS and CSIRO, and they are currently carrying out a project in China that can synthesize and use a large number of existing data. The USGS library provides a total of 2,457 spectra targeting not only minerals but also various materials that respond to infrared radiation. Among these, there are 1,276 mineral spectra, which are about half of the total. The spectrum title includes information, such as analysis devices (NIC4, BECK, ASDNG, etc.), purity codes (a, b, c, d, u), and measurement methods (AREF, RREF, RTGC, TRAN). Analyzed raw data are provided in ASCII and GIF format. The CSIRO library has a total of 502 spectra, of which the majority, 493, correspond to mineral spectra. The USGS library is a free, publically available resource, while the CSIRO library is bundled with TSG8 or must be purchased separately. Among these, when comparing the eight spectra whose spectral shapes can be analyzed with the spectra of domestic illite, the positions of the absorption peaks are significantly different from those of domestic illite, except for one Japanese illite. Additional research will be needed to determine the causes of such differences, and the domestically relevant databases should be established as well.

• BMB Reports
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• v.40 no.5
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• pp.644-648
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• 2007

Exposure of algae or plants to irradiance from above the light saturation point of photosynthesis is known as high light stress. This high light stress induces various responses including photoinhibition of the photosynthetic apparatus. The degree of photoinhibition could be clearly determined by measuring the parameters such as absorption and fluorescence of chromoproteins. In cyanobacteria and red algae, most of the photosystem (PS) II associated light harvesting is performed by a membrane attached complex called the phycobilisome (PBS). The effects of high intensity light (1000-4000 ${\mu}mol$ photons $m^{-2}s^{-1}$) on excitation energy transfer from PBSs to PS II in a cyanobacterium Spirulina platensis were studied by measuring room temperature PC fluorescence emission spectra. High light (3000 ${\mu}mol$ photons $m^{-2}s^{-1}$) stress had a significant effect on PC fluorescence emission spectra. On the other hand, light stress induced an increase in the ratio of PC fluorescence intensity of PBS indicating that light stress inhibits excitation energy transfer from PBS to PS II. The high light treatment to 3000 ${\mu}mol$ photons $m^{-2}s^{-1}$ caused disappearance of 31.5 kDa linker polypeptide which is known to link PC discs together. In addition we observed the similar decrease in the other polypeptide contents. Our data concludes that the Spirulina cells upon light treatment causes alterations in the phycobiliproteins (PBPs) and affects the energy transfer process within the PBSs.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.315-326
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• 2010

This study examined the overlapping resonances in the systems involving 1 open and 2 closed channels using the phase-shifted version of multichannel quantum-defect theory (MQDT). The results showed that 21 patterns for the q reversals in the autoionization spectra are possible depending on the relative arrangements of the two simple poles and roots of the quadratic equations. Complete cases could be generated easily using the q zero planes determined using only 3 asymmetric spectral line profile indices. The transition of the spectra of the coarse interloper Rydberg series from the lines into a structured continuum by being dispersed onto the entire Rydberg series was found. The overall behavior of the time delays was found to be governed by the dense Rydberg series, which is quite different from the one of the autoionization cross sections that is governed by an interloper, indicating that different dynamics prevail for them. This is in contrast to the two channel system where both quantities behave similarly. The dynamics obtained in the presence of overlapping resonances is as follows. The absorption process is instant and dominated by a transition to the interloper line. This process is followed by rapid leakage into the dense Rydberg series, which has a longer residence time before ionization than that of the interloper state. This is because the orbiting period is proportional to $\upsilon^3$ so that an excited electron has a shorter lifetime in the interloper state belonging to a lower member of the Rydberg series.

• Current Photovoltaic Research
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• v.4 no.1
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• pp.1-7
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• 2016

Among the various types of solar cells, silicon based two terminal tandem solar cell is one of the most popular one. It is designed to split the absorption of incident AM1.5 solar radiation among two of its component cells, thereby widening the wavelength range of external quantum efficiency (EQE) spectra of the device, in comparison to that of a single junction solar cell. In order to improve the EQE spectra further and raise short circuit current density ($J_{sc}$) an optimization of the tradeoff between the top and bottom cell is needed. In an optimized cell structure, the $J_{sc}$ and hence efficiency of the device can further be enhanced with the help of light trapping scheme. This can be achieved by texturing front and back surface as well as a back reflector of the device. In this brief review we highlight the development of light trapping in the silicon based tandem solar cell.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.193-199
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• 2010

An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the $sll0396{\Omega}$ mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin ${\beta}$- and ${\alpha}$-subunits and lacked the 30- and 32-kDa linker polypeptides, and northern blot analysis revealed that the transcription of the 1.4-kb cpcBA gene encoding the phycocyanin ${\beta}$- and ${\alpha}$-subunits was lower in the mutant. The Sll0396 protein has a DNA-binding motif and shares homology with known response regulators. Our results indicate that Sll0396 plays a regulatory role in the transcription of the phycocyanin genes during phycobilisome synthesis.

• Resources Recycling
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• v.7 no.1
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• pp.7-13
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• 1998

The electric arc furnace (EAF) dust used in this experiment showed the formation of an irregular agglomerate of small spherical particles and consisted of 27.5% Fe, 18% Zn, 4.83% Pb and 10.2% CaO, and it also contained 3.26% Cl and 0.15% F. IR spectra peaks of Pb(OH)Cl were observed at 1630 and 1377 ${cm}^{-1}$ regions and the intensity decreased using an acid treatment (1N ${H}_{2}{SO}_{4}$) and thermal treatment at above 600℃, but it was not affected by washing process. Those of ${Zn}_{5}{(OH)}_{8}{Cl}_{2}$ treated by oxidation showed broad absorption peak at 3600∼3200 ${cm}^{-1}$, main peaks at 1136 and 1040 ${cm}^{-1}$ and a splitting peak at 991 and 921 ${cm}^{-1}$ which were believed to be due to ZnO by oxidation at about 700∼. The IR spectra of the dust residues by a washing process show the same peaks at 3449, 1635, 1439, 875, 571 and 455 ${cm}^{-1}$ regions, but the peaks at 2942, 2862, 1136 and 1040 ${cm}^{-1}$ decreased and disappeared using an acid treatment and washing process, which is believed to be due to the dissolution of zinc hydroxyl chloride.