• Title/Summary/Keyword: Zen

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Promotion of in vitro shoot proliferation in rose by addition of liquid medium to culture (액체배지 첨가에 의한 장미 기내 신초 증식 촉진)

  • Lee, Ye Ji;Lee, Jung Lim;Hyung, Nam-In;Kim, Seung Tae;Lee, Eun Kyung;Kwon, O Hyeon;Kim, Won Hee;Lee, Su Young
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.305-308
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    • 2012
  • To promote the growth and proliferation of in vitro rose (Rosa hybrida L) shoots, a liquid medium was added to shoot culture. Shoots were obtained by culturing internodes of four cultivars, 'Antique Curl', 'Shiny Orange', 'White Zen', and 'Red Zen', and then were proliferated by the subculture two times. An addition with 10~15 mL of liquid medium enhanced the shoot elongation of all four cultivars. However, the effect of liquid medium addition to culture of in vitro shoot for proliferation was dependent on cultivars of rose.

The Meaning of Wangwei's Natural Beauty and His Garden, Wangchuan Villa (왕유의 자연미와 망천별업의 조경사적 의의)

  • 박희성;조정송
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.2
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    • pp.1-13
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    • 2001
  • Beginning in the Wei-Chin and Liu Ch´ao era(AD. 220-589), an awareness of natural beauty emerged as the major interest of scholars which created such a social atmosphere that served as the foundation of prosperous Chinese art. During the T´ang period, the desire to achieve natural beauty began to dominate every field of art including gardening. This study covers the T´ang period when scholars began to be conscious of aesthetics. The main objective of this study is to examine the significance of natural beauty as interpreted by Wangwei, an artist representative of the period, and of Wangchuan Villa in relation to aspects of garden theory. This study is mainly based on Wangwei´s anthology, landscape painting describing Wangchuan Villa, and historical records related to the Villa. The summary of conclusions of this study is as follows: 1) Wangwei´s concept of natural beauty is a combination of objective natural beauty and pastoral beauty, which stresses the essential beauty of nature. Moreover, he considered nature as a religious Utopia. 2)Wangchuan Villa, where Wangwei stayed until his death, was a place of seclusion representing his idea of natural beauty. There, he was able to realize the paradise of Buddhism full of zen aspirations. 3)From the perspective of garden theory, Wangchuan Villa can be classified as a suburb villa in terms of location, as a landscape garden in terms of elements, and as a villa based on a typical private land system in terms of forms. In addition, it may be considered as a garden that laid the foundation to realize a field of non-visible image and non-visible landscape, which is fundamental element of scholarly garden design.

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Autometallography for Zinc Detection in the Central Nervous System (중추신경계통내 분포하는 Zinc의 조직화학적 동정)

  • Jo, Seung-Mook;Gorm, Danscher;Kim, Sung-Jun;Park, Seung-Kook;Kang, Tae-Cheon;Won, Moo-Ho
    • Applied Microscopy
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    • v.30 no.4
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    • pp.347-355
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    • 2000
  • Zinc is one of the most abundant oligoelements in the living cell. It appears tightly bound to some metalloproteins and nucleic acids, loosely bound to some metallothioneins or even as free ion. Small amounts of zinc ions (in the nanomolar range) regulate a plentitude of enzymatic proteins, receptors and transcription factors, thus rolls need accurate homeostasis of zinc ions. Zinc is an essential catalytic or structural element of many proteins, and a signaling messenger that is released by neural activity at many central excitatory synapses. Growing evidences suggest that zinc may also be a key mediator and modulator of the neuronal death associated with transient global ischemia and sustained seizures, as well as perhaps other neurological disease stoles. Some neurons have developed mechanisms to accumulate zinc in specific membrane compartment ('vesicular zinc') which can be evidenced using histochemical techniques. Substances giving a bright colour or emitting fluorescence when in contact with divalent metal ions are currently used to detect them inside cells; their use leads to the so called 'direct' methods. The fixation and precipitation of metal ions as insoluble salt precipitates, their maintenance along the histological process and, finally, their demonstration after autometallographic development are essential steps for other methods, the so called 'indirect methods'. This study is a short report on the autometallograhical approaches for zinc detection in the central nervous system (CNS) by means of a modified selenium method.

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Dispersion of Standing Stones at Noseongsan(Mt.Noseong) and Aspect of the Stone Decorated Garden(Soo-suk Jeongwon) at Chongsuk-Sa(Chongsuk Buddhist Temple) in Nonsan City (논산 노성산(魯城山)의 입석(立石) 분포와 총석사(叢石寺) 수석(樹石)의 정원적 면모)

  • Rho, Jae Hyun;Huh, Joon;Jang, Il Young
    • Korean Journal of Heritage: History & Science
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    • v.43 no.1
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    • pp.160-189
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    • 2010
  • This study has been designed to grasp the present situation, shapes and meaning of the standing stones and rock pillars in the whole area of Noseong Mountain Fortress in Nonsan City which have never been academically reported yet. Accordingly, the research was carried out to grasp the spatial identity of Noseong Mt. and Noseong Mountain Fortress and the dispersion of standing stones scattered around inside and outside Noseong Mountain Fortress, while the shapes and structural characteristics of stones were investigated and analyzed focusing on Chongsuk Temple, which was considered to have the highest density of standing stones and greatest values for preservation as a cultural property. In consideration of the reference to the 'Top Sa' (tower temple) at the 'Bul Woo Jo' (Article about Buddhism Houses) of 'Shinjoong Dongguk Yeoji Seungram', theoretical existence of the temple according to surveying investigation, and the excavation records of roof tile pieces with the name of 'Gwan Eum Temple', it is presumed that there had been a Buddhist sanctum inside the fortress and it could be connected to the carved letters, 'Chongsuk Temple'. According the observation survey, the 6th place of standing stones among many other places inside the fortress shows that Chongsuk Temple appears to have the strong characteristics of artificially constructed space in consideration of the size of trees and stones, the composite trend of tree and stone composition, and trace of the adjacent well and strand and the construction of stairway leading to the stone gate. Along with the constellation of the Big Dipper carved on a rock at the same space, the stones, on which the letters of 'Shinseonam', 'Chilseongam' and 'Daejangam' were carved, including 'Chongsuksa', and the carved statue of Buddha, which was assumed to be Avalokitesvara Guan Yin, have offered clue which make it possible to infer that the space was a space for Chilseong and Mountain god(Folk Belief) that had originated from the combination of Buddhism, Taoism and folk religion. According to the actual measurement of standing stones at Chonsuk Temple, it was identified that there were big differences in height among 24 stones in total, ranging from 402~29cm and the averaged distance between each stone appeared to be 23.6cm. And the shape of stones appeared to be standing or flat, and various stones such as mountain-like stones and Buddha-like stones were placed in a special arrangement or assorted arrangement, but the direction of the stones had a consistency pointing to the west. And comparing to the trace of construction of ZEN Landscape Garden well known in the country, the three flat stones except for the standing and shaped stones appeared to have the shape of meditation statue, which is the typical formational factors of a ZEN Landscape Garden, on the basis of formational technique of stones. Among them, the flat stone facing the Buddhist saint statue, was formed by way of symbolization of three-mountain stone, which was assumed to be an offering stone for sacrificial food rather than carrying out ZEN Meditation. In consideration of the formation of standing stones at Chong-suk Temple, which was carried out in the composite stoning method based using the scalene triangle with ratio of 3:5:7 in order to seek the in-depth beauty based on the stone statues of three Buddhas where the three factors such as heaven, earth and humans are embodied in the elevated or flat formation, the stones at Chongsuk Temple and the space seemed to the trace of contracted garden construction that was formed with stones for a temple, so that could be used for ZEN meditation.

On Study for the JIT System By CIM(Computer Integrated Mfg) (JIT실현을 위한 CIM구축 사례연구)

  • Lee, Jong-Hyung;Lee, Youn-Heui
    • Journal of the Korean Society of Industry Convergence
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    • v.7 no.4
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    • pp.425-432
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    • 2004
  • This study for Customer Satisfaction(Customer Focus) by Profit security' in the field Process improvement activity and man-power upgrade in the learning of organization activity or upgrading ability of each peoples. This thesis study on the focus of KAPEC which introduce Toyota system can apply to VM, 3jeong, Right Box and Right Position), 5S, JIT(Just In Time), KAlZEN, KANBAN System, CIM, ERP, DAS an output of Factory. For strategic changes to take place in industry 3 key important factors need to be included ; integration of tasks functions and process, decentralization of information and responsibility and finally simplification of products and product structures. These describes how CIM can be implemented using these factors. This study for (1)System Integration, (2) Help Logistic Problems, (3) Partly facilitated growth. (4) Improved production planning (5) Real-time management. (6) Fast reporting (7) Productivity. Quality. Delivery Up, Cost reduction and Autonomy management, FMS in the Plant etc.

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Superconducting Tunnel Junction Detectors for Mass Spectrometry

  • Ohkubo, M.;Zen, N.;Kitazume, T.;Ukibe, M.;Shiki, S.;Koike, M.
    • Progress in Superconductivity
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    • v.14 no.2
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    • pp.77-81
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    • 2012
  • With conventional mass spectrometry (MS), ions are separated according to mass/charge (m/z) ratios. We must speculate the z values to obtain the m values. Superconducting tunnel junction (STJ) detectors can solve this problem, and true mass spectrometry becomes possible instead of m/z spectrometry. The STJ detectors were installed in MS instruments with a variety of ion sources. As an example, we report fragmentation analysis of a non-covalent protein complex of hemoglobin.

Harmonics-based Spectral Subtraction and Feature Vector Normalization for Robust Speech Recognition

  • Beh, Joung-Hoon;Lee, Heung-Kyu;Kwon, Oh-Il;Ko, Han-Seok
    • Speech Sciences
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    • v.11 no.1
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    • pp.7-20
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    • 2004
  • In this paper, we propose a two-step noise compensation algorithm in feature extraction for achieving robust speech recognition. The proposed method frees us from requiring a priori information on noisy environments and is simple to implement. First, in frequency domain, the Harmonics-based Spectral Subtraction (HSS) is applied so that it reduces the additive background noise and makes the shape of harmonics in speech spectrum more pronounced. We then apply a judiciously weighted variance Feature Vector Normalization (FVN) to compensate for both the channel distortion and additive noise. The weighted variance FVN compensates for the variance mismatch in both the speech and the non-speech regions respectively. Representative performance evaluation using Aurora 2 database shows that the proposed method yields 27.18% relative improvement in accuracy under a multi-noise training task and 57.94% relative improvement under a clean training task.

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On the Design of the Latch Mechanism for Wafer Containers in a SMIF Environment

  • Lee, Jyh-Jone;Chen, Dar-Zen;Pai, Wei-Ming;Wu, Tzong-Ming
    • Journal of Mechanical Science and Technology
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    • v.20 no.12
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    • pp.2025-2033
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    • 2006
  • This paper presents, the design of a latch mechanism for wafer containers in a standard mechanical interface environment. For an integrated circuits fabrication factory, the standard mechanical interfaced wafer container is an effective tool to prevent wafers from particle contamination during wafer storage, transporting or transferring. The latch mechanism inside the container door is used to latch and further seal the wafer container for safety and air quality. Kinematic characteristics of the mechanism are established by analyzing the required functions of the mechanisms. Based on these characteristics, a methodology for enumerating feasible latch mechanisms is developed. New mechanisms with one degree-of-freedom and up to five links are generated. An optimum design is also identified with respect to the criteria pertinent to the application. The computer-aided simulation is also built to verify the design.

An Effect of Information Society on Contemporary Fashion Design (정보화 사회가 현대 의상디자인에 미친 영향에 대한 연구)

  • 이유경
    • Journal of the Korea Fashion and Costume Design Association
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    • v.1 no.1
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    • pp.35-51
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    • 1999
  • This study was aimed to analyze how images of information society were embodied in contemporary fashion design. To find out images of information society expressed in contemporary fashion design, this paper reviewed the characteristics of information society, and, found out six categories of contemporary fashion design in which the information society was expressed. Cyber environment causes techno style clothing which is characterized by the metallic or lustrous material and mechanical message. And interest in new materials increased significantly. Time-space compression makes the meaning of time and space change, therefore the past, present and future are coexist in the contemporary fashion design. The boundaries has loosed, so, heterogeneous materials and colors are used in fashion design all together. Variety and plurality affect expression of individuality and self-concept. Flexibility increased, so, soft and pliable clothing increased, too. Finally, quality of life is considered as more important value than quantity, so, luxurious look and zen style appear. Also, minimalism which is characterized as simplicity and purity affects the contemporary fashion design.

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Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy

  • Wang, Tzi-Yuan;Tsai, Yi-Hsuan;Yu, I-Zen;Chang, Te-Sheng
    • Journal of Microbiology and Biotechnology
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    • v.26 no.3
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    • pp.498-502
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    • 2016
  • 3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'-hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.