• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1769-1776
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• 2004

The aim of this study was to find out the effects of the Yukmijihwangtang on transcription activity of Genes related to Bone Morphogenesis. For this purpose, experiments were performed to compare the polyphosphate contents of Yukmijihwangtang and its component herbs, and to verify their Effects on transcription activity of Genes related to Bone Morphogenesis. We know that Yukmijihwangtang and its component herbs have adequate amount of polyphosphate contents and have effects on transcription activity of Genes such as BMP1A, BMP2B, OTN, MGP, COL. In the conclusion, Yukmijihwangtang and its component herbs are strongly believed to have effectiveness on bone morphogenesis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.896-902
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• 2008

Yukmijihwang-tang(YM) is used in Oriental medicine for treatments of diverse systemic symptoms including neurological dosorders. The present study was performed to examine potential effects of YM on growth-promoting activity of injured sciatic nerve axons. YM treatment in the injured sciatic nerve induced enhanced distal elongation of injured axons when measured 3 and 7 days after injury. Retrograde tracing of sciatic nerve axons showed YM-mediated increases in the number of DiI-labeled dorsal root ganglion (DRG) sensory neurons and spinal cord motor neurons at 3 days after injury. Hoechst nuclear staining showed that non-neuronal cell population was largely elevated by YM treatment in distal nerve area undergoing axonal regeneration. Furthermore, phospho-Erk1/2 protein levels were upregulated by YM treatment in the injured nerve area. These data suggest that YM may play a role in facilitated axonal regeneration in injured peripheral nerves. Further investigations of individual herbal components would be useful to explore effective molecular components and develop therapeutic strategies.

• Herbal Formula Science
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• v.17 no.2
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• pp.65-72
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• 2009

Objectives : Yukmijihwang-tang (YJT) is known as a tonifying formula for reinforcement of yin deficiency conditions. The present study was carried out to investigate the potential acute toxicity of YJT in ICR male and female mice. Methods : We investigated the acute toxicity about boiling water-extracted YJT. The test article was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000 mg/kg body weight. Mortalities, clinical findings, autopsy and body weight changes were monitored daily for the 14 days following the administration according to the Regulation of Korean Food and Drug Administration. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. Single oral administration of YJT with different dosages, no animals died of the test drug. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of YJT for ICR mice was more than 5000 mg/kg on oral route. Conclusions : These results suggest that no toxic dose level of YJT in mice is considered to be more than 5000 mg/kg. Consequently, it was concluded that YJT have no effect on acute toxicity and side effect in ICR mice.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.6
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• pp.498-502
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• 2015

Yukmijihwang-tang (YM) is a well-known prescription to treat kidney inflammation on Korea. However, the effects of YM on maximal exercise performance (MEP) is not-well investigated. In this study, we aimed to evaluate the effects of YM on MEP using forced swimming test (FST) model. Mice were largely divided into three group; a non-swimming group, saline treated-swimming load test group, and YM treated-swimming load group. Swimming load test groups were re-divided into swimming control group (Control), YM fed group (YM). YM was administrated orally for 2 weeks before FST. After FST, immobility time, oxygen consumption was measured by physiological test, and serum was collected for biochemical analysis. FST induced MEP identified by increased immobility, oxygen consumption, and biochemical factors. Immobility time was significantly reduced by YM treatment compared with control group. Oxygen consumption was also significantly reduced by YM treatment. The increase on lactic acid and lactate dehydrogenase after FST was inhibited by YM　treatment. In addition, consumption of energy source (free fatty acid, and triglyceride) and recovery of energy was enhanced by YM treatment after FST. In conclusion, YM inhibited the elevation of immobility time, oxygen consumption, and biochemical factors after FST. YM could be used as a beneficial agents to enhance maximal endurance exercise performance and an effective ergogenic aid in exercise training.

• Journal of Oriental Neuropsychiatry
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• v.29 no.4
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• pp.207-221
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• 2018

Objectives: The objective of this study was to observe the anti-amnesic effects of Yukmijihwang-tang (YMJHT), on the scopolamine (Sco)-induced memory impairment in C57BL/6 mice through its favorable acetylcholine (ACh). Also, to observe acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect. Methods: Six groups, with a total of 20 normal and 100 Sco treated mice were selected based on their body weights after 1 week of acclimatization, were used in this study as follows. Half of the mice in each group were used for passive avoidance task tests and hippocampus ACh content, AChE activity and ChAT mRNA expression measurement, and the remaining half in each group used for Morris water maze test and measurement of cerebral antioxidant defense system. Results: Amnesia due to AChE activations and destroyed cerebral cortex antioxidant defense systems were markedly and dose-dependently inhibited after 28 days of continuous oral pre-treatment with YMJHT 400, 200 and 100 mg/kg, respectively. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg. Conclusions: Based on the results, it was established that oral administration of YMJHT favorably alleviates Sco-induced memory impairment, through preservation of ACh, mediated by up-regulation of ChAT mRNA expressions and related AChE inhibition and augmentation of cerebral antioxidant defense system, at least in a condition of this experiment. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.2
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• pp.359-364
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• 2002

The present study examined the effects of water extract of Hwangruynhaedoktang plus Yukmijihwangtang add Chogudeung (HYC) on the blood pressure and renal function in spontaneously hypertensive rats (SHR). The HYC water extract blocked increase of systolic blood pressure (SBP) in SHR. The urinary volume (UV), sodium (UNaV), potassium (UkV), and chloride (UclV) excretions were significantly increased in SHR administered with high dose of water extract of HYC, but urinary osmolality was not changed. The creatinine clearance (Ccr), which is index of glomerular filtration rate (GFR), was increased by administration of HYC, while solute-free water reabsorption (TcH2O) was not changed. Taken together, these results suggest that the depressor effects of HYC, at least in part, caused by diuretic effect due to increase of glomerular filtration rate.

• The Journal of Internal Korean Medicine
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• v.42 no.4
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• pp.628-644
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• 2021

Objectives: This study analyzed, through network analysis and data mining analysis, the relationship between herbs used in osteoporosis prescriptions, diversified the analysis of osteoporosis-related prescriptions, and analyzed the combination of herbs used in osteoporosis-related prescriptions. Methods: The prescriptions used in osteoporosis treatment and experiments were established by conducting a full survey of the papers published by the OASIS site. A database for osteoporosis-related prescriptions was established, herbs were extracted, and the frequency of frequent herbs and prescriptions were investigated using Excel (MS offices ver. 2013). Using the freeware R version 4.0.3 (2020-10-10), igraph, and arules package, network analysis was performed in the first second of prescription composition. Results: Among the osteoporosis-related prescriptions, the most studied prescriptions are as follows.: Yukmijihwang-tang (六味地黃湯) and Samul-tang (四物湯). In the osteoporosis prescription network, herbs with connection centrality, proximity centrality, mediation centrality, and eigenvector centrality appeared in the order of Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Poria Sclerotium, Paeoniae Radix, and Glycyrrhizae Radix et Rhizoma. After extracting the herbal combination network, including the corresponding herbs, and clustering it, it can be divided into drugs of the Yukmijihwang-tang (六味地黃湯) series and the Samul-tang (四物湯). Conclusions: This study could assist researchers in diversifyingy formula analysis in future studies. Moreover, the herbal combination used in osteoporosis prescriptions could be used to search for osteoporosis prescriptions in other databases or to create a new prescription.

• Herbal Formula Science
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• v.9 no.1
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• pp.405-417
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• 2001

Objective : Rett syndrome is a neurological disorder occurring primarily in girls. By process of treatment for a case which was diagnosed as Rett syndrome and out patient department treatment from the 12th, April, 1999 to November, 2001, the results are as follows. Method & Results : This patient was diagnosed as five-development disorders categories(五遲, 五軟), medicated with Yukmijihwang-tang(Liuweidihwang-tang, 六味地黃湯) and Kyejigayonggolmoryu-tang(Guizhijialonggu-tang, 柱枝加龍骨牡蠣湯). And acupunture therapy was taken on Shinjyungkyuk(shenchongge, 腎正格) and Paekoe(百會 GV 20), Taechu(大椎 GV 14), Renzhong(人中 GV 26). As a result, the patient's hypotonia and intermittent seizures were improved. Conclusion: Rett syndrome is most often misdiagnosed as autism, cerebral palsy or non-specific developmental delay. In oriental medicine, RS can be diagnosed as five development disorders categories (五遲, 五軟) and oriental medication with acupuncture treatment can improve RS patient's conditions.

• The Journal of Internal Korean Medicine
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• v.41 no.2
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• pp.248-255
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• 2020

Objectives: Fatigue, malnutrition, and weight loss are common complaints after undergoing surgery for cancer. These symptoms can be associated with Yin-Deficiency (陰虛) of Exhaustion syndrome (虛勞). Therefore, this study aimed to determine if it is possible to use Dokhwaljihwang-tang, which originates from Yukmijihwang-tang, for enhanced recovery after cancer surgery. Methods: After cancer surgery, Dokhwaljihwang-tang-gami was administered three times a day, in 80-120 ml doses at a time. Results: In four cases of gastric, colorectal, and thyroid cancer, enhanced recovery was achieved using Dokhwaljihwang-tang-gami to treat general weakness, fatigue, and pain in the surgical site after cancer surgery. Conclusions: Dokhwaljihwang-tang can be used for enhanced recovery after cancer surgery.

• The Journal of Internal Korean Medicine
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• v.40 no.2
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• pp.254-261
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• 2019

Internuclear Ophthalmoplegia (INO) is characterized by lesion side eye's adduction limitation in lateral horizontal gaze and nystagmus in the abducting eye due to lesions in the Medial Longitudinal Fasciculus (MLF). Brainstem infarction is one of the causes of INO, but related issues have seldom been reported in the Korean medical literature. The present case was a pontine infarction patient with INO who complained of diplopia and eye movement disorder. The patient was treated with Korean medicine treatment, including herbal medicine, acupuncture, cupping, and moxibustion for 30 days. Her changes in clinical symptoms were measured with a Numeric Rating Scale (NRS) and a length of External Ocular Movement (EOM), in which the patient's right eyeball moved to the left from the midline in the left gaze. After treatment, her clinical symptoms were improved. Diplopia decreased from NRS 10 to NRS 2, and eye movement disorder was improved such that EOM increased from 1mm to 5 mm. Therefore, integrative Korean medicine treatment may be effective in the treatment of INO patients caused by pontine infarction.