Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Anti-amnesic Effect and Antioxidant Defense Systems of Yukmijihwang-tang on Scopolamine-induced Memory Impairment in Mice

Scopolamine 유발 건망증 마우스 모델에서 육미지황탕(六味地黃湯)의 기억력 개선 및 항산화 효과

  • Seo, Young-Min (Department of Oriental Neuropsychiatry, College of Korean Medicine, Daegu Hanny University) ;
  • Han, Da-young (Department of Oriental Neuropsychiatry, College of Korean Medicine, Daegu Hanny University) ;
  • Kim, Sang-ho (Department of Oriental Neuropsychiatry, College of Korean Medicine, Daegu Hanny University) ;
  • Chung, Dae-kyoo (Department of Oriental Neuropsychiatry, College of Korean Medicine, Daegu Hanny University)
  • 서영민 (대구한의대학교 한의과대학 신경정신과교실) ;
  • 한다영 (대구한의대학교 한의과대학 신경정신과교실) ;
  • 김상호 (대구한의대학교 한의과대학 신경정신과교실) ;
  • 정대규 (대구한의대학교 한의과대학 신경정신과교실)
  • Received : 2018.10.16
  • Accepted : 2018.12.05
  • Published : 2018.12.31
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Abstract

Objectives: The objective of this study was to observe the anti-amnesic effects of Yukmijihwang-tang (YMJHT), on the scopolamine (Sco)-induced memory impairment in C57BL/6 mice through its favorable acetylcholine (ACh). Also, to observe acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect. Methods: Six groups, with a total of 20 normal and 100 Sco treated mice were selected based on their body weights after 1 week of acclimatization, were used in this study as follows. Half of the mice in each group were used for passive avoidance task tests and hippocampus ACh content, AChE activity and ChAT mRNA expression measurement, and the remaining half in each group used for Morris water maze test and measurement of cerebral antioxidant defense system. Results: Amnesia due to AChE activations and destroyed cerebral cortex antioxidant defense systems were markedly and dose-dependently inhibited after 28 days of continuous oral pre-treatment with YMJHT 400, 200 and 100 mg/kg, respectively. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg. Conclusions: Based on the results, it was established that oral administration of YMJHT favorably alleviates Sco-induced memory impairment, through preservation of ACh, mediated by up-regulation of ChAT mRNA expressions and related AChE inhibition and augmentation of cerebral antioxidant defense system, at least in a condition of this experiment. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg.

Keywords

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Fig. 1. Experimental Design Used in This Study.

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Fig. 1. Experimental Design Used in This Study.

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Fig. 2. Body Weight Changes in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 2. Body Weight Changes in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 3. Changes on the Step-through Latency Times of Passive Avoidance Task Test in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 3. Changes on the Step-through Latency Times of Passive Avoidance Task Test in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 4. Changes on the Escape Latency Times of Morris Water Maze Test in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 4. Changes on the Escape Latency Times of Morris Water Maze Test in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 5. Changes on the Hippocampus ACh Contents in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 5. Changes on the Hippocampus ACh Contents in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 6. Changes on the Hippocampus AChE Activities in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 6. Changes on the Hippocampus AChE Activities in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 7. Changes on the Hippocampus ChAT mRNA Expressions in Normal Mice and Sco-induced Amnesia Mice.

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Fig. 7. Changes on the Hippocampus ChAT mRNA Expressions in Normal Mice and Sco-induced Amnesia Mice.

Table 1. Composition of YMJHT Used in This Study

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Table 1. Composition of YMJHT Used in This Study

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Table 2. The Specific Sequence for Each Primer

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Table 2. The Specific Sequence for Each Primer

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Table 3. Changes on the Body Weight Gains in Normal Mice and Scopolamine-Induced Amnesia Mice

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Table 3. Changes on the Body Weight Gains in Normal Mice and Scopolamine-Induced Amnesia Mice

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Table 4. Cerebral Cortex Antioxidant Defense Systems in Normal Mice and Scopolamine-induced Amnesia Mice

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Table 4. Cerebral Cortex Antioxidant Defense Systems in Normal Mice and Scopolamine-induced Amnesia Mice

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