• Title/Summary/Keyword: Young root

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Studies on the amino acid metabolism of young rice root (Part 4) -Properties of glutamic oxalacetic transaminase isozymes of rice root- (수도근(水稻根)의 Amino 산(酸) 대사(代謝)에 관(關)한 연구(硏究) -제(第) 4 보(報) 수도근(水稻根)의 Glutamic Oxalacetic Transaminase Isozyme의 성질(性質))

  • Kim, Kwang Sik;Kim, Young Ung
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.4
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    • pp.209-213
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    • 1974
  • The isozymes of glutamic oxalacetic transaminase from young rice root were isolated by DEAE-cellulose column chromatography, and investigated some of their enzymic properties. Obtained results were summarized as follows: 1. 93 percent of GOT activity in rice root existed in supernatant fraction, and their specific activity exibited the same pattern. 2. The rice root contained two types of GOT isozyme; cationic and anionic GOT. It seemed that both of them were distributed in supernatant fraction. 3. GOT isozymes of rice root on the pH dependance showed their maximum activity at 7.4. 4. On the stability of GOT isozymes for temperature, anionic GOT was more unstable than cationic GOT. 5. Apparent Michaelis constant for L-aspartate of GOT isozymes from rice root showed 12-14mM in the cationic GOT and 16mM in the anionic GOT, respectively.

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Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

  • Kim, Won-Jung;Jung, Hee-Young;Min, Ji-Yun;Chung, Young-Gwan;Lee, Cheol-Ho;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.372-377
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    • 2004
  • Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Comparison of Growth Characteristics of 1- and 2-year-old Bare Root and Container Seedling of Chamaecyparis obtusa (편백 1, 2년생 노지묘와 용기묘의 생장특성 비교)

  • Song, Ki Sun;Cha, Young Geun;Choi, Jin Young;Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.101 no.2
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    • pp.317-323
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    • 2012
  • This study was performed in order to explore the growth characteristics by growing stage in the containerized seedling and the bare root seedling of Chamaecyparis obtusa Sieb. et Zucc., which is the major plantation species of Korean southland. The height growth of 1-year-old bare root seedlings was better than containerized seedlings, which grew in the containers with capacity of 31.2 mL and 300 mL. The root collar diameter growth was the best in the containerized seedlings of container with 300 mL. The 2-year-old bare root seedlings were surveyed to be better in the height and root collar diameter growth than the containerized seedlings of container with 300 mL. In the comparison of dry mass production, it was the highest in 32 mL containerized seedlings as for 1-year-old above ground and in 300 mL containerized seedlings as for below-ground and total dry mass production. In case of 2-year-old seedlings, it was indicated to be high in bare root seedling as for the part above ground and in dry mass production of the containerized seedling as for the part below ground. In the comparison on root morphological traits of seedlings such as the total root length, the root project area, the root surface area, the average diameter in root, and the root volume, all of 1 and 2-year -old containerized seedlings showed the higher growth than the bare root seedlings except the average diameter in root. Synthesizing the results of this experiment, the containerized seedlings are relatively excellent in root development compared to bare root seedlings in the process of producing seedlings of C. obtusa, thereby being judged to have grown into excellent seedlings.

Growth and Chlorophyiil Biosynthesis of Vigna angularis under Lead Stress

  • Koo Suh-Young;Jin Sun-Young;Hong Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.2
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    • pp.145-155
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    • 1997
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna angularis seedlings. Expose to 50 uM Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of Al reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. $\delta-Aminolevulinic$ acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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A STUDY ON THE PHYSICAL PROPERTIES OF ROOT CANAL SEALERS (근관충전용 실러의 물리적 성질에 관한 연구)

  • Chang, Young-In;Choi, Ho-Young
    • Restorative Dentistry and Endodontics
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    • v.20 no.1
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    • pp.142-151
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    • 1995
  • The purpose of this study was to compare and estimate the physical properties of five root canal sealers classified Calciobiotic root canals sealer as calcium hydroxide based sealer, Apatite root sealer type II as calcium phosphate based sealer, AH-26 as resin based sealer, Canals and Pulpdent root canals sealer as zinc oxide eugenol based sealer. The author investigated dimensional change and flow rate of canal sealers, diametral tensile strength and shear bond strength of sealers to dentin to evaluate the physical properties on affect of complete obturation of root canal and performed the total 100 specimens of each 25 sealers under the condition of root temperature according to manufacturer's instructions. All specimens were stored at $37{\pm}1^{\circ}C$ in 100 % relative humidity. A microscope for measurement of micro distance is used for the dimensional change test and evacuation methods using vaccum were used for the flow rate test. The result differed by the storage time measured on the tests of diametral tensile strength and shear bond strength to dentin. The following results were obtained ; 1 On the test of dimensional change, Canals and Pulpdent expanded slightly, AH-26 and Apatite showed the severe shrinkage after 48 hours. 2. AH-26 and Apatite were the excellent with each 24.59mm, 31.19mm after 3 minutes in the aspect of flow property. 3. On the diametral tensile strength, Calciobiotic root canals sealer showed the highest strength with 27.13kg/$cm^2$ after 48 hours, Apatite root sealer type II showed highest strength with 84.57kg/$cm^2$ after 120 hours. 4. On the shear bond strength to dentin, AH-26 was most excellent with 55.73kgf/$cm^2$ after 24 hours and with 134.71kgf/$cm^2$ after 120 hours.

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Studies on Selection of Adaptable Varieties in Paddy - Field of Ginseng Culture (인삼 논재배에 적응하는 품종 선발에 관한 연구)

  • Kang, Seung-Weon;Lee, Sung-Woo;Hyun, Dong-Yun;Yeon, Byeong-Yeol;Kim, Young-Chang;Kim, Young-Chul
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.6
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    • pp.416-420
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    • 2010
  • Root yield and quality of ginseng cultured in paddy soil was low relatively compared with that of upland soil because of moisture injury in root during rainy season. Drainage class in soils generally divided into 6 classes, and it is possible to cultivate ginseng practically in imperfectly drainage class (IDC). This study carried out to select the varieties that is suitable for paddy soil, which is easy to be generated rusty-colored root and physiological-discolored leaf. Experiment plot arranged with the condition of soil humidity contents such as poorly drainage class (PDC) and imperfectly drainage class (IDC), and upland soil. Growth characteristics and root yield were investigated in four-year-old ginseng of varieties, Cheonpoong (CP), Yeonpoong (YP), Hwangsookjong (HS), and Jakyeongjong (JK). CP among four varieties showed the highest yield in IDC and CP was the lowest ratio in leaf discoloration and rusty-colored root. HS was followed by CP in the order of root yield, but it had the weakness that the ratio of rusty-colored root was high respectively.

Studies on the Variation of Metabolites, Especially on the Amino Acid Composition of Root-portion During the Growth Period in Soybean Young Plant (대두유식물의 발육에 따른 뿌리의 부위별 체내성분 특히 아미노산조성의 변이에 관한 연구)

  • 강영희
    • Journal of Plant Biology
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    • v.20 no.1
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    • pp.15-22
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    • 1977
  • As a basic research for the yield-mechanism of soybean seeldings, the distribution and variety of free amino acid and protein-amino acid in some parts of root were studied. At the meristematic, elongational and maturated zones of root, the distribution and variety of N-components was different substantially. It shows that N-components are taken part in energy source for organization in early stage of growth. N-pool also changed in every part of root.

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Influence of Rice-Root-Nematode(Hirschmanniella aryzae) on the Root Browning of Rice (벼뿌리의 갈변에 미치는 벼뿌리선충의 영향)

  • Lee Young-Bae;Park Jung-Soo
    • Korean journal of applied entomology
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    • v.14 no.1 s.22
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    • pp.35-36
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    • 1975
  • An experiment was carried out to know bow the rice root nematode, Hirschmanniella cryzae affects the root browning of rice pant growing in various combinations of media. In sterilized conditions of growing media, the root browning of rice plant was slightly promoted by the presence of the rice root nematode, while the entire roots were stained to brown in nonsterilized conditions of growing media. It seems that the root browning is due mainly to soil micro-organisms other than nematode which promotes it slightly under sterilized conditions.

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A Study on the Effects of Rhodiola rosea Root on the Immune System (홍경천(紅景天)의 면역증진(免疫增進) 효능(效能)에 대한 연구(硏究))

  • Kim, Jung-Yeal;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.23 no.4
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    • pp.179-189
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    • 2008
  • Objectives: Effects of Rhodiola rosea root on immunity were examined. Methods: Water extracts and methanol extracts of Rhodiola rosea root were treated on the increase of immunity cells and the activation of cytokines were examined. Results: 1. Water extracts and methanol extracts of Rhodiola rosea root had positive effects on the increase of B-cells, T-cells, and NK cells, and on the facilitation of cytokines such as IL-6 and TNF-${\alpha}$. 2. Water extracts and methanol extracts of Rhodiola rosea root activated NK cells, and induced the generation of NO- of NK cells and macrophages. Conclusions: Rhodiola rosea root can be used to increase immunity.

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A Study on the Effects of Rhodiola rosea Root on the Cancers (홍경천(紅景天)(Rhodiola rosea Root)의 항암(抗癌)효과에 대한 연구(硏究))

  • Kim, Jung-Yeal;Seong, Nak-Sull;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.21 no.1
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    • pp.79-87
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    • 2006
  • Objectives : Effects of Rhodiola rosea root on cancers on stomach, breast, lung, and liver were examined. Methods : Water extracts and methanol extracts of Rhodiola rosea root were treated on cancer cells, and its effects on cancers were examined. Results : 1. Water extracts and methanol extracts of Rhodiola rosea root was less harmful in its lowest density 0.25 mg/mL (9.l%와 10.5%), but became more harmful as its density increased. 2. As for human stomach adenocarcinoma cells AGS, breast adenocarcinoma cells MCF-7, and lung carcinoma cells A549, methanol extracts showed 70-77% inhibition of cancer cells in high density(1 mg/mL), and water extracts showed 60-70% inhibition rate and its selective death rate was less than 2.5. Conclusion : Rhodiola rosea root can be used to treat cancers and to increase immunity.

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