• Journal of Embryo Transfer
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• v.30 no.3
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• pp.225-228
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• 2015

Understanding the behavior of transgenes introduced into oocyte or embryos is essential for evaluating the methodologies for transgenic animal production. To date, many studies have reported the production of transgenic pig embryos with, however, low efficiency in environment of blastocyst production. The aim of present study was to determine the expression and duration of transgene transferred by intracytoplasmic sperm injection-mediated gene transfer (ICSI-MGT). Embryos obtained from the ICSI-MGT procedure were analysed for the expression of GFP and then for the transmission of the transgene. Briefly, fresh spermatozoa were bound to exogenous DNA after treatment by Triton X-100 and Lipofectin. When ICSI-MGT was performed using sperm heads with tails removed, the yield of blastocyst (25.3%), treated with Lipofectin (18.8%) and Triton X-100 (19.2%) were observed. Treatments of Lipofectin or Triton X-100 did not further improve the rates of blastocysts. Moreover, the apoptosis rates of embryos were obtained from the control and LIpofectin groups (8.7%, 9.7%, respectively), but were significantly higher in the Triton X-100 group (13.0%). Our results demonstrated that ICSI-MGT caused minimal damage to oocytes that could develop to full term. Moreover, the embryos derived by ICSI-MGT have shown prolonged exogenous DNA expression during preimplantation stage in vivo. However, more efforts will be required to improve the procedures of both sperm treatments cause of high frequency of mosaicisms.

• Transactions of the Society of Information Storage Systems
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• v.2 no.2
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• pp.130-137
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• 2006

The HDD(hard disk drive) using Load/unload(L/UL) technology includes the benefits which are increased areal density, reduced power consumption and improved shock resistance than those of contact-start-stop(CSS). It has been widely used in portable hard disk drive and will become the key technology for developing the small form factor hard disk drive. The main objects of L/UL are no slider-disk contact or no media damage. For realizing those, we must consider many design parameters in L/UL system. In this paper, we focus on lift-off force. The 'lift-off' force, defined as the minimum air bearing force, is another very important indicator of unloading performance. A large amplitude of lift-off force increases the ramp force, the unloading time, the slider oscillation and contact-possibility. To minimize 'lift-off' force we optimizes the slider and suspension using the integrated optimization frame, which automatically integrates the analysis with the optimization and effectively implements the repetitive works between them. In particular, this study is carried out the optimal design considering the process of modes tracking through the entire optimization processes. As a result, we yield the equation which can easily find a lift-off force and structural optimization for suspension.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.129-135
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• 2013

This study was conducted to investigate the soil characteristics of newly reclaimed tidal land and the effect of green manure crops on soil properties. Summer green manure crops such as sesbania (Sesbania grandiflora), barnyard grass (Echinochloa spp.) and sorghum${\times}$sudangrass hybrid (Sorghum bicolor L.) were cultivated at Hwaong, Ewon, Saemangeum and Yongsangang area. Soil pH of reclaimed tidal land was relatively high, but organic matter and available phosphorus contents were lower compared to the optimum range for common upland crops. Soil nutrient contents were unbalanced for upland crop growth. Yield of green manure crops had a wide spatial variation. Nitrogen content in green manure crops was the greater in Sesbania and it was estimated that major nutrient ($N-P_2O_5-K_2O$) supply amount were 150-40-370, 220-50-170 and 140-50-250 $kg\;ha^{-1}$ from sorghum${\times}$sudangrass hybrid, sesbania and barnyard grass, respectively. Based on these results, desalination is required to grow the upland crops at newly reclaimed tidal lands and management practices are necessary to reduce the salt damage by resalinization during the growing seasons. To improve the productivity and increase the nutrient utilization rate, soil physicochemical properties need to be improved to the level for upland crops by application of organic matter and fertilizer.

• Journal of Korea Water Resources Association
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• v.40 no.1 s.174
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• pp.1-10
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• 2007

Reallocation procedure of multipurpose reservoir storage capacity between flood control and conservation is presented as an alternative to secure more water resources. Storage reallocation is an adaptive management mechanism for converting existing normal pool level of reservoirs to more beneficial uses without requirement for physical alteration. This study is intended to develop a reservoir storage reallocation methodology that allows increased water supply storage without minimizing adverse impacts on flood control. The methodology consists of flood control reservoir simulation for inflows with various return periods, flow routing from reservoir to a potential damage site, analyzing river carrying capacity, and reservoir yields estimation for reallocated storages. For the flood control model, a simulation model called Rigid ROM(Reservoir Operation Method) and HEC-5 are used. The approach is illustrated by applying it to two reservoirs system in Geum River basin. Especially with and without new project conditions are considered to analyze trade-offs between competing objectives.

• Biomolecules & Therapeutics
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• v.28 no.1
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• pp.34-44
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• 2020

Mesenchymal stem cells (MSCs) have been proposed as an alternative therapy to be applied into several pathologies of the nervous system. These cells can be obtained from adipose tissue, umbilical cord blood and bone marrow, among other tissues, and have remarkable therapeutic properties. MSCs can be isolated with high yield, which adds to their ability to differentiate into non-mesodermal cell types including neuronal lineage both in vivo and in vitro. They are able to restore damaged neural tissue, thus being suitable for the treatment of neural injuries, and possess immunosuppressive activity, which may be useful for the treatment of neurological disorders of inflammatory etiology. Although the long-term safety of MSC-based therapies remains unclear, a large amount of both pre-clinical and clinical trials have shown functional improvements in animal models of nervous system diseases following transplantation of MSCs. In fact, there are several ongoing clinical trials evaluating the possible benefits this cell-based therapy could provide to patients with neurological damage, as well as their clinical limitations. In this review we focus on the potential of MSCs as a therapeutic tool to treat neurological disorders, summarizing the state of the art of this topic and the most recent clinical studies.

• International Journal of Naval Architecture and Ocean Engineering
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• v.10 no.2
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• pp.153-169
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• 2018

A submarine has a pressure hull that can withstand high hydraulic pressure and therefore, requires the use of highly advanced shipbuilding technology. When producing a pressure hull, periodic inspection, repair, and maintenance are conducted to maintain its soundness. Of the maintenance methods, Non-Destructive Testing (NDT) is the most effective, because it does not damage the target but sustains its original form and function while inspecting internal and external defects. The NDT process to detect defects in the welded parts of the submarine is applied through Magnetic particle Testing (MT) to detect surface defects and Ultrasonic Testing (UT) and Radiography Testing (RT) to detect internal defects. In comparison with RT, UT encounters difficulties in distinguishing the types of defects, can yield different results depending on the skills of the inspector, and stores no inspection record. At the same time, the use of RT gives rise to issues related to worker safety due to radiation exposure. RT is also difficult to apply from the perspectives of the manufacturing of the submarine and economic feasibility. Therefore, in this study, the Phased Array Ultrasonic Testing (PAUT) method was applied to propose an inspection method that can address the above disadvantages by designing a probe to enhance the precision of detection of hull defects and the reliability of calculations of defect size.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.6
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• pp.3-10
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• 2018

This paper described the shear strengthening effect by deviator location in pre-damaged reinforced concrete (RC) beams strengthened with externally post-tensioning steel rods. Three reinforced concrete beams as control beam and eight post-tensioned beams using external steel rods were tested to fail in shear. The externally post-tensioning material was a steel rod of 22 mm diameter, and it had a 655 MPa yield strength and an 805 MPa tensile strength. Specimens depend on multiple variables, such as the number of deviators, location of deviator, and load pattern. The pre-damaged loads up to about 2/3 of ultimate shear capacities were applied to specimens using displacement control and the diagonal shear crack just occurred at these loading levels. And then, the post-tensioning up to when a strain of steel rod reaches about $2000{\mu}{\varepsilon}$ was continuously applied to beam. A displacement control was changed to a load control during post-tensioning. The post-tensioning resulted in increase of load-carrying capacity and restoration of existing deflection. Also, it prevented the existing diagonal cracks from excessively growing. Two deviators effectively improved the load capacity when compared with in case of test which one deviator at mid-span installed. When deviators were located near region which the diagonal crack occurred on, the strengthening impact by post-tensioning was greater.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1876-1884
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• 2020

Ethanol often accumulates during the process of wine fermentation, and mitophagy has critical role in ethanol output. However, the relationship between mitophagy and ethanol stress is still unclear. In this study, the expression of ATG11 and ATG32 genes exposed to ethanol stress was accessed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The result indicated that ethanol stress induced expression of the ATG11 and ATG32 genes. The colony sizes and the alcohol yield of atg11 and atg32 were also smaller and lower than those of wild type strain under ethanol whereas the mortality of mutants is higher. Furthermore, compared with wild type, the membrane integrity and the mitochondrial membrane potential of atg11 and atg32 exhibited greater damage following ethanol stress. In addition, a greater proportion of mutant cells were arrested at the G1/G0 cell cycle. There was more aggregation of peroxide hydrogen (H2O2) and superoxide anion (O2•-) in mutants. These changes in H2O2 and O2•- in yeasts were altered by reductants or inhibitors of scavenging enzyme by means of regulating the expression of ATG11 and ATG32 genes. Inhibitors of the mitochondrial electron transport chain (mtETC) also increased production of H2O2 and O2•- by enhancing expression of the ATG11 and ATG32 genes. Further results showed that activator or inhibitor of autophagy also activated or inhibited mitophagy by altering production of H2O2 and O2•. Therefore, ethanol stress induces mitophagy which improves yeast the tolerance to ethanol and the level of mitophagy during ethanol stress is regulated by ROS derived from mtETC.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.313-322
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• 2022

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and important for plant health and productivity. In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. A total of 87 bacterial isolates and 66 fungal isolates were obtained. Sequencing of 16S rDNA and internal transcribed spacer amplicon showed that these isolates correspond to 30 and 15 different species of bacteria and fungi, respectively. Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. Our collection of soybean seed endophytes would be a valuable resource not only for studying biology and ecology of seed endophytes but also for practical deployment of seed endophytes toward crop protection.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.1
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• pp.133-152
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• 2022

Rice is a fundamental staple food commodity all around the world. Globally, it is grown over 167 million hectares and occupies almost 1/5^th of total cultivated land under cereals. With a total production of 782 million metric tons in 2018. In Pakistan, it is the 2^nd largest crop being produced and 3^rd largest food commodity after sugarcane and rice. The stem borers a type of pest in rice and other crops, Scirpophaga incertulas or the yellow stem borer is very serious pest and a major cause of yield loss, more than 90% damage is recorded in Pakistan on rice crop. Yellow stem borer population of rice could be stimulated with various environmental factors which includes relative humidity, light, and environmental temperature. Focus of this study is to find the environmental factors changes i.e., temperature, relative humidity and rainfall that can lead to cause outbreaks of yellow stem borers. this study helps to find out the hot spots of insect pest in rice field with a control of farmer's palm. Proposed system uses temperature, relative humidity, and rain sensor along with artificial neural network to predict yellow stem borer attack and generate warning to take necessary precautions. result shows 85.6% accuracy and accuracy gradually increased after repeating several training rounds. This system can be good IoT based solution for pest attack prediction which is cost effective and accurate.