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Human Papillomavirus Distribution among Women in Western Shandong Province, East China using Reverse Blot Hybridization Assay

  • Lee, Dongsup;Kim, Geehyuk;Kim, Sunghyun;Park, Sunyoung;Wang, Hye-young;Park, Sangjung;Han, Lin;Yubo, Ren;Li, Yingxue;Park, Kwang Hwa;Lee, Hyeyoung
    • Biomedical Science Letters
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    • v.21 no.2
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    • pp.69-76
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    • 2015
  • Cervical cancer is the third most common cancer in women worldwide and there is a significant association between human papillomavirus (HPV) infection and cervical cancer. Certain HPV groups, labeled high-risk (HR) HPV groups, are strongly associated with malignancies of the human cervix. HPV prevalence and genotype distribution were analyzed using the REBA $HPV-ID^{(R)}$ (YD Diagnostics, Yongin, Korea) assay based on the reverse blot hybridization assay (REBA) with a total of 324 liquid-based cytology samples from women in Western Shandong Province, East China and results were compared with cytological diagnosis. Most of the HPV genotypes that were detected in high-grade cervical lesions were HR-HPV genotypes such as HPV 16, 18, 33, 53, and 58. The prevalence of these HR-HPV genotypes increased in high-grade cervical lesions. However, from low- to high-grade cervical lesions, the ability to detect LR-HPV genotypes decreased. Additionally, in general, the single HPV genotype infection rate increases in proportion to the severity of the lesion. The study findings suggest that a currently available preventive vaccine against HPV 16 and 18 may have limited effectiveness for prevention of all HPV infection in this province. Finally, based on these findings, these data could guide national or regional vaccination programs in the Western Shandong Province of East China to substantially reduce the burden of cervical lesions.

Dyeabilities of Elastic Composite Yarn Woven Fabrics (탄성복합사를 이용한 직물의 염색성에 대한 연구)

  • Kim, Ji-Yeon;Kim, Sang-Wook;Min, Mun-Hong;Lee, Sang-Bong;Park, Yeon-Bong;Kang, Shin-Hyeok;Yeum, Jeong-Hyun
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.108-108
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    • 2012
  • 이전의 연구에서 기존의 ATY 장치에 ITY 노즐을 접목하여 개조한 사가공기로 제조한 탄성복합사를 자동차 시트용 트리코트 원단으로 편직하여 일광견뢰도가 우수한 염료를 선정하고 $125^{\circ}C$에서 염색하여야 견뢰도와 spandex의 물성을 저해하지 않음을 알 수 있었다. 폴리에스테르 탄성복합사의 염색성 평가에 이어 본 연구에서는 나일론 필라멘트 (70/24, Full-dull, 2ply사와 40D spandex 1ply사 복합)와 레이온사(75D, 1ply)를 투입하여 직물 원단(중량 216g/yd, 밀도 경사 78, 위사 52본/inch)을 제직한 후 2욕에서의 염색공정을 거칠 경우, 탄성복합사의 견뢰도와 물성에 미치는 영향을 조사하였다. 나일론, 레이온, 폴리우레탄의 3종 원단을 염색성 평가를 위하여 반응성염료로 먼저 레이온 부분을 염색한 후 산성염료로 나일론 부분을 염색하였으며, 이 때 spandex가 미치는 영향을 비교하기 위하여 spandex가 함유되지 않은 나일론, 레이온 복합 제직원단도 함께 염색한 후 견뢰도를 평가하였다. 또한, 레이온 부분은 CPB염색법과 제트염색기로 나누어 염색하여 염색기에 따른 인장강도와 인열강도를 평가하였다. 레이온 염색에는 셀룰로오스용 2관능기 반응성염료 3원색을 이용하여 khaki color로 combination염색하였으며, 나일론 부분에는 입자크기가 커서 견뢰도가 우수한 산성염료를 선정하여 combination염색한 후 냉수세하였다. 견뢰도를 비교한 결과, spandex가 포함된 탄성복합사 원단과 나일론과 레이온 만으로 제직된 원단 모두 세탁, 마찰, 물, 땀, 일광 견뢰도 모두가 4~5급으로 우수하여 spandex에 이염된 염료가 견뢰도에 미치는 영향은 없음을 확인하였다. 또한, 레이온 부분의 반응성염료 염색 시 CPB와 제트염색법의 두 가지 종류에 대한 인장강도와 인열강도 평가결과는 CPB 염색물이 제트염색물보다 약간 높게 나타났지만 3% 이내의 차이로 거의 차이가 없음을 나타내었다. 일반적으로 제트염색 시 원단은 로프상으로 이동하고, CPB염법은 확포상태 그대로 염색되기 때문에 제트염색 시 강도가 낮아지는 것으로 알려져 있으나, 본 연구에서는 탄성복합사를 경사, 위사 모두 사용함으로써 spandex가 신장하는 특성 때문에 강도의 저하가 없는 것으로 사료된다.

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Enhanced Variable Structure Control With Fuzzy Logic System

  • Charnprecharut, Veeraphon;Phaitoonwattanakij, Kitti;Tiacharoen, Somporn
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.999-1004
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    • 2005
  • An algorithm for a hybrid controller consists of a sliding mode control part and a fuzzy logic part which ar purposely for nonlinear systems. The sliding mode part of the solution is based on "eigenvalue/vector"-type controller is used as the backstepping approach for tracking errors. The fuzzy logic part is a Mamdani fuzzy model. This is designed by applying sliding mode control (SMC) method to the dynamic model. The main objective is to keep the update dynamics in a stable region by used SMC. After that the plant behavior is presented to train procedure of adaptive neuro-fuzzy inference systems (ANFIS). ANFIS architecture is determined and the relevant formulation for the approach is given. Using the error (e) and rate of error (de), occur due to the difference between the desired output value (yd) and the actual output value (y) of the system. A dynamic adaptation law is proposed and proved the particularly chosen form of the adaptation strategy. Subsequently VSC creates a sliding mode in the plant behavior while the parameters of the controller are also in a sliding mode (stable trainer). This study considers the ANFIS structure with first order Sugeno model containing nine rules. Bell shaped membership functions with product inference rule are used at the fuzzification level. Finally the Mamdani fuzzy logic which is depends on adaptive neuro-fuzzy inference systems structure designed. At the transferable stage from ANFIS to Mamdani fuzzy model is adjusted for the membership function of the input value (e, de) and the actual output value (y) of the system could be changed to trapezoidal and triangular functions through tuning the parameters of the membership functions and rules base. These help adjust the contributions of both fuzzy control and variable structure control to the entire control value. The application example, control of a mass-damper system is considered. The simulation has been done using MATLAB. Three cases of the controller will be considered: for backstepping sliding-mode controller, for hybrid controller, and for adaptive backstepping sliding-mode controller. A numerical example is simulated to verify the performances of the proposed control strategy, and the simulation results show that the controller designed is more effective than the adaptive backstepping sliding mode controller.

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Surface Display of Bacillus CGTase on the Cell of Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Bacillus CGTase의 표층발현)

  • Kim Hyun-Chul;Lim Chae-Kwon;Kim Byung-Woo;Jeon Sung-Jong;Nam Soo-Wan
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.118-123
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    • 2005
  • For the expression in Saccharomyces cerevisiae, Bacillus stearothermophilus cyclodextrin glucano­transferase gene (cgtS) in pCGTS (4.8 kb) was subcloned into the surface expression vector, pYD1 (GALl promoter). The constructed plasmid, pYDCGT (7.2 kb) was introduced into S. cerevisiae EBY100 cells, and then yeast transformants were selected on the synthetic defined media lacking tryptophan. The formation of cyclodextrin (CD) was confirmed with active staining of culture broth of transformant grown on starch medium. Enzymatic reaction products with respect to the culture time and the reaction time were examined by TLC analysis. The results indicated that the enzyme activity was exhibited after 12 h cultivation and CD was produced after 10min of enzymatic reaction. When the surface-engineered yeast cells were cultured on galactose medium, maximum activities of CGTase were about 21.3 unit/l and 16.5 unit/l at $25^{\circ}C\;and\;30^{\circ}C$, respectively. The plasmids stability showed about $80\%\;even\;at\;25^{\circ}C\;and\;30^{\circ}C$.

Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts

  • Lee, Dong-Woon;Kim, Jae-Gu;Kim, Meyoung-Kon;Ansari, Sahar;Moshaverinia, Alireza;Choi, Seong-Ho;Ryu, Jae-Jun
    • The Journal of Advanced Prosthodontics
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    • v.7 no.2
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    • pp.138-145
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    • 2015
  • PURPOSE. The objective of this study was to conduct an in vitro comparative evaluation of polished and laser-dimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. MATERIALS AND METHODS. Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had $5-{\mu}m$ dimples at $10-{\mu}m$ intervals, and SM30 (n=16) had $5-{\mu}m$ dimples at $25-{\mu}m$ intervals in a $2{\times}4mm^2$ area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. RESULTS. The adhesion strength of epithelial cells was higher on Ti surfaces with $5-{\mu}m$ laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. CONCLUSION. These findings demonstrate that laser dimpling may contribute to improving the peri-implant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

A Study on Exothermic Properties of TiO2/Epoxy Nanocomposites (TiO2/Epoxy 나노복합재의 발열 특성에 관한 연구)

  • Ahn, Seok-Hwan;Ha, Yoo-Sung;Moon, Chang-Kwon
    • Journal of Ocean Engineering and Technology
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    • v.27 no.5
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    • pp.99-104
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    • 2013
  • Recently, various nanoparticles have been used for filler in polymer matrices. The particles of nano size are whether high or not cross-link density in polymer affects the thermal and mechanical properties of one. The properties change as a result of chemical reactions between the nanoparticles and the surface of the polymer. There are two models for nanocomposites: "repulsive interaction" and "attractive interaction" between the nanoparticles and matrix. In this study, the variation in the curing mechanism was examined when nano-size $TiO_2$ was dispersed into an epoxy (Bisphenol A, YD-128) with different curing agents. The results of this study showed that the exothermic temperature and Tg in the case of the nanoparticles used (Jeffamine) (D-180) at room temperature were reduced by an increase in the $TiO_2$ contents because of the "repulsive interaction" between the nanoparticles and the matrix. The tensile strengths were increased by increasing amounts of $TiO_2$ until 3 wt% because of a dispersion strengthening effect caused by the nanoparticles, because of the repulsive interaction. However, such tensile properties decreased at 5 wt% of $TiO_2$, because the $TiO_2$ was agglomerated in the epoxy. In contrast, in the case of the nanoparticles that used NMA and BDMA, the exothermic temperature and Tg tended to rise with increasing amounts of $TiO_2$ as a result of the "attractive interaction." This was because the same amounts of $TiO_2$ were well dispersed in the epoxy. The tensile strength decreased with an increase in the $TiO_2$ contents. In the general attractive interaction model, however, the cross-link density was higher, and tensile strength tended to increase. Therefore, for the nanoparticles that used NMA, it was difficult to conclude that the result was caused by the "attractive model."

Changes in Anthocyanin Content of Aronia (Aronia melancocarpa) by Processing Conditions (물리적 처리조건 변화에 따른 아로니아(Aronia melancocarpa) 유래 안토시아닌 함량변화 특성)

  • Kim, Bo Mi;Lee, Kyung Min;Jung, In Chan
    • Korean Journal of Plant Resources
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    • v.30 no.2
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    • pp.152-159
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    • 2017
  • The purpose of this study was to obtain basic data for using Aronia as a functional food material. The composition of anthocyanin was characterized and quantitated by LC-MS/MS, HPLC, and UV-VIS spectrophotometer techniques, respectively. The anthocyanin content was analyzed by temperature, time, pH, and the addition of citric acid. The UV-VIS spectrophotometer used for analysis of anthocyanin is less accurate than the LC-MS/MS method used in recent years. In the past, cyanidin-3-Glucoside was reported to be a major anthocyanin that contains Aronia. However, LC-MS/MS analysis in this study confirmed cyanidin-3-galactoside to be the major compound. The anthocyanin content of the Aronia powder began to decrease sharply at a temperature of $65^{\circ}C$ or higher when heated for 24 hours. In an aqueous solution of Aronia, the anthocyanin content was reduced by 50% at $65^{\circ}C$ for 10 hours and decreased by 85% at $85^{\circ}C$ within 10 hours. Above pH 8, the anthocyanin content was reduced by more than 50%. The results of this study will provide useful information to maintain anthocyanin content in the manufacturing process of Aronia. It could also be used to ensure the stability of anthocyanins in similar species of berries.

A Study on Long-term Variations of BOD and COD as Indicators of Organic Matter Pollution in the Han River (한강 본류에서 유기물 오염도 지표인 BOD와 COD에 대한 장기변동 특성)

  • Cho, Hyun-Seok;Kim, Kwang-Rae;Lim, Gyu-Chul;Bae, Kyung-Seok;Lee, Min-Hwan
    • Korean Journal of Ecology and Environment
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    • v.45 no.4
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    • pp.474-481
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    • 2012
  • This study was performed to investigate the degree of long-term pollution at the mainstream of the Han River by comparing the concentration of BOD and COD from 1975 to 2011. The long-term annual average BOD and COD concentration at the mainstream of the Han River showed an increasing trend as it flowed downstream from Paldang Dam to Gayang. The concentration of BOD ($r^2$=0.646) and COD ($r^2$=0.260) showed a consistent decreasing trend for 37 years. In the case of Paldang Dam, BOD has maintained a decreasing trend, whereas the COD value showed an increasing trend after the 1990s. Therefore, a control of non-biodegradable materials in areas around Paldang Dam is required. The result of the seasonal variations of BOD and COD is as follows: spring>winter>summer and fall (p<0.001). The time series analysis revealed a strong correlation for every 12-month period. Also, the amount of water discharge at Paldang Dam has to be systematically controlled because the amount of water discharge from the dam influences the water quality at the mainstream of the Han River.

Distyly and Population Size of Abeliophyllum distichum Nakai, an Endemic Plant in Korea (한국 특산식물 미선나무의 이화주성(Distyly) 및 개체군 크기)

  • So-Dam Kim;Ae-Ra Moon;Shin-Young Kwon;Seok-Min Yun;Hwi-Min Kim;Dong-Hyoung Lee;Sung-Won Son
    • Korean Journal of Environment and Ecology
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    • v.36 no.6
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    • pp.639-650
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    • 2022
  • Abeliophyllum distichum Nakai, a rare plant with distylous characteristics, is native to certain parts of the Korean Peninsula. It is registered on the IUCN Red List of Threatened Species as a globally endangered plant. This study was conducted to establish an appropriate local conservation management plan suitable for future A. distichum populations by comparing and analyzing the flowering characteristics and population size according to distyly based on the results of quantitative surveys in 14 regions, including 8 areas with native populations of A. distichum and 6 natural monument populations. The number of individuals appearing in each population group was surveyed, and the flowering individuals were identified by style as being either pin or thrum flower types as they were being examined and recorded on the site. In total, 13,130 individuals of A. distichum (7,003 flowering and 6,127 non-flowering individuals) were recorded, but the balance of the number of pin- and thrum-flowered individuals in each population was not significant (p<0.05), indicating an imbalanced state. In particular, the Yeongdong (YD) population was very disproportionate compared to other populations, suggesting that its genetic diversity was low and the possibility of inbreeding was high. The average flowering and fruiting rates by management unit were much higher in the natural monument populations (89.2% and 55.3%, respectively) than in the natural habitat populations (39.0% and 8.5%, respectively). It may be due to a difference in reproductive growth resulting from light inflow into the forest caused by the upper crown closure. The area of occupation (AOO) of A. distichum on the Korean Peninsula covered an area of 23,224.5 m2. Although the natural monument population was smaller than the natural habitat population, its density was higher, likely as a result of the periodic management of natural monument populations, where the installation of protective facilities in certain areas restricts population spread. Conservation of A. distichum populations requires removing the natural monument populations suspected of anthropogenic and genetic disturbances and expanding the conservation priority population by designating new protected areas. Although the habitats of natural monument populations are managed by the Cultural Heritage Administration and local governments, there are no agencies that are responsible for managing natural habitat populations. Therefore, institutional improvement in the overall management of A. distichum should be prioritized.

Comparison of the Growth Performance of 12 Crossbred Korean Native Chickens and Commercial Layer from Hatch to 16 Weeks (12개의 토종닭 교배조합과 실용 산란계의 육성기 성장능력 비교)

  • Eunsoo Seo;Myunghwan Yu;Elijah Ogola Oketch;Shan Randima Nawarathne;Nuwan Chamara Chathuranga;Bernadette Gerpacio Sta. Cruz;Venuste Maniraguha;Jun Seon Hong;Doo Ho Lee;Minjun Kim;Jung Min Heo
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.303-310
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    • 2023
  • The current study was conducted to compare the effect of crossbred on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 873 one-day-old chicks were divided into twelve crossbreds (i.e., CFCK, CFYC, CFYD, CKCF, CKYC, CKYD, YCYD, YCCF, YCCK, YDCF, YDCK, and YDYC) and commercial layer (Hy-Line Brown) were obtained as a counterpart in the study. All the birds are raised in battery cages (76 × 61 × 46 cm3) and then raised until 14 weeks and subsequently moved to layer battery cages (60 × 25 × 45 cm3) and raised until 16 weeks. The body weight and viability were measured biweekly from hatching to week 16. The week of 16, body weight range was about 1,010.24 to 1,411.77 g. The body weight of specific crossbreeds (i.e., CKCF, YCYD, and YDYC) was found to be comparable to that of Hy-Line Brown). The viability hatch to week 14 range was about 55 to 100% and however week 14 to 16 range was 80 to 100%. The crossbred (i.e., CKCF) recorded superior (P<0.05) viability throughout the whole experiment period, even compared with Hy-Line Brown (100% vs 96%). Our results are indicating that crossbreds Korean native chicken including CKCF, and YDYC has the potential to enhance key features of laying hens during the growing phase like optimal body weight and higher viability.