• Korean Journal of Microbiology
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• v.30 no.1
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• pp.30-36
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• 1992

nif (nitrogen fixation)-H.D, K genes of Frankia sp. SNU 014201. a symbiotic strain isolated from root nodule of Alnus hirsura, were found to be located in the genome on 13.5 kb of EcoRI, 18.0 kb of BamHI, 10.5 kb of BglII and 4.5 kb of KpnI fragments. Using EMBL-3 BamHI arms of bacteriophage lambda. the genomic library was constructed. from which fourteen recombinant phage nif-clones were selected. Among them, Ahnif-I2 had insert DNA of 18 kb, in which 7.9 kb of BamHl fragment contained nif-H, D, K and 3.6 kb of HindlIl/KpnI had nif-H and partial -D. Therefore, the 7.9 kb and 3.6 kb fragments were subcloned and partial restriction maps were constructed. As the results, nif-F1, D.K genes were found to be located continuously on the 6.5 kb of HindII/BamHI and 5.2 kb of SalIIBamHI fragment in the genome of Frankia sp. SNU 014201.

• Journal of Korean Library and Information Science Society
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• v.31 no.4
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• pp.213-237
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• 2000

The pnpose of the study is to offa the basic data in TQM dcation Ilndel fca h i thrcugh investjga!iOn of m s tlxm-dic hackgnxnd, such as philosophy, pincides, mil2 am？s, aL&atirn mlhods and d y s i s of TQM &cation for h i . For this pnpose, phil&y and pinddes that reprrsentive quality expats in America and jam^, such as bring, Juran, G-osby, Ishkawa, M, wae investigated

• Journal of Information Management
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• v.9 no.2
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• pp.43-49
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• 1976

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1696-1701
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• 2010

The ${\alpha}$-amylases activity was improved by random mutagenesis and screening. A region comprising residues from the position 34-281 was randomly mutated in B. licheniformis ${\alpha}$-amylase (AmyL), and the library with mutations ranging from low, medium, and high frequencies was generated. The library was screened using an effective liquid-phase screening method to isolate mutants with an altered pH profile. The sequencing of improved variants indicated 2-5 amino acid changes. Among them, mutant TP8H5 showed an altered pH profile as compared with that of wild type. The sequencing of variant TP8H5 indicated 2 amino acid changes, Ile157Ser and Trp193Arg, which were located in the solvent accessible flexible loop region in domain B.

• Korean Journal of Microbiology
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• v.42 no.4
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• pp.246-251
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• 2006

A lot of mutants which cannot initiate sexual development were screened and several loci including nsdA, nsdB, nsdC, and nsdD were identified in homothallic ascomycetes Aspergillus nidulans. The NSD206, which has nsdC6 allele, showed typical phenotype of NSD (Never in sexual development) mutants. The nsdC gene was cloned by transforming NSDP697 ($nsdC^-$, $pryG^-$) with AMA1-NotI genomic library. The transforming library DNA recovered from several transformants showing wild phenotype carried about 10 kb genomic DNA insert. The DNA sequence of nsdC was analysed using GPS (Genome priming system). The nsdC gene has an open reading frame (ORF) of 1,929 bp encoding a putative polypeptide of 643 amino acids. The NsdC carries $C_2H_2C_2H_2C_2HC$ type zinc finger DNA binding domains in the middle of the polypeptide. A coiled-coil domain at its C terminus were also found. In nsdC6 allele, a single T insertion was occurred between 407-408 bp leading to the frameshift mutation and early termination of translation producing the truncated protein which has only 139 amino acids.

• Journal of the Korean Society for Library and Information Science
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• v.45 no.2
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• pp.119-143
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• 2011

This study identifies the characteristics of recent citation-based indicators for assessing a single paper in the context of their co-relationships. Five predefined indicators were examined with three variants of h-index which are convened in this study; the formers are PageRank, SCEAS Rank, CCI, f-value, and single paper h-index and the latters are $h_S$-index, h1-index, and $h_S$1-index. The correlation analysis and cluster analysis were performed to group the indicators by common characteristics, after which the indicators were calculated with the dataset from KSCI DB. The results show statistical evidence that distinguishes h-index type indicators from others. The characteristics of the indicators were verified with citation frequency factors using correlation analysis. Finally, the implications for applications and further studies are discussed.

• Molecules and Cells
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• v.27 no.2
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• Journal of Korean Library and Information Science Society
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• v.47 no.2
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• pp.287-304
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• 2016

This paper introduces a new bibliometric measure called Quality Factor, which assesses multiple facets of faculty research performance. The computation of Quality factor is based on a combination of publication count, citation count, h-index, and Impact Factor. In order to analyze the relationship between Quality Factor and other bibliometric measures (publication count, citation count, h-index, g-index, Impact Factor), the study collected publication data of 189 Korean Library and Information Science professors from 2001 to 2014 to produce the rankings of the faculty by each bibliometric measure and computed Spearman's rank correlations between the rankings. The overall results showed Quality Factor to be correlated to citation-driven measures (citation count, h-index, g-index), but the scatterplot as well as rank-interval analysis showed Quality Factor to be distinctive and more discriminating than other measures.

• Journal of Korean Library and Information Science Society
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• v.22
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• pp.95-137
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• 1995

Christianity Newspapers related to Korea was started from 'The Christian Advocate' launched in 1897 by A ppenzeller, H.G, American. From here, through the division of the newspaper related to Korean Christianity, the periodic background of the time, contents of the newspaper published and the bibliographic feature are as follows. 1. The time of Introduction (1885-1910) 2. The time of Establishment (1911-1934) 3. The time of sufferings (1935-1945) 4. The time of upheaval (1946-1960) 5. The time of preparation

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.507-513
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• 2017

Bacillus subtilis spores can be used for protein display to engineer protein properties. This method overcomes viability and protein-folding concerns associated with traditional protein display methods. Spores remain viable under extreme conditions and the genotype/phenotype connection remains intact. In addition, the natural sporulation process eliminates protein-folding concerns that are coupled to the target protein traveling through cell membranes. Furthermore, ATP-dependent chaperones are present to assist in protein folding. CotA was optimized as a whole-cell biocatalyst immobilized in an inert matrix of the spore. In general, proteins that are immobilized have advantages in biocatalysis. For example, the protein can be easily removed from the reaction and it is more stable. The aim is to improve the pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. A library of approximately 3,000 clones was screened. A E498G variant was identified to have a half-life of inactivation ($t_{1/2}$) at pH 4 that was 24.8 times greater compared with wt-CotA. In a previous investigation, a CotA library was screened for organic solvent resistance and a T480A mutant was found. Consequently, T480A/E498G-CotA was constructed and the $t_{1/2}$ was 62.1 times greater than wt-CotA. Finally, E498G-CotA and T480A/E498G-CotA yielded 3.7- and 5.3-fold more product than did wt-CotA after recycling the biocatalyst seven times over 42 h.