• The Plant Pathology Journal
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• 제24권4호
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• pp.453-460
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• 2008

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, $6.5{\times}10^6$ and $2.5{\times}10^7$ spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duck-weed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of $12.5{\mu}g$ per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broad-spectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity ($a_w$) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

• The Plant Pathology Journal
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• 제24권2호
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• pp.178-182
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• 2008

Five essential oils and 15 herbal extracts were evaluated to control Bursaphelenchus xylophillus in laboratory. The essential oils from clove plant (Syzygium aromaticum), mustard (Brassica integrefolia), thyme (Thymus vulgaris), and Pelargonium inquinans were found to be highly promising and gave excellent control of the nematodes at all the time of exposure. Among them, the least one gave 91.3% mean mortality rate at 24 hours of exposure time, which is highly significant from the control. While in the second study, most of the methanol (Desmodium caudatum, Paulownia coreana, Auckulandia lappa, Sophota flavescens, Aloe sp., Rheum palmatum, Zingiber officinale, Magnolia officinalis, and Eugenia caryophyllata), hexane (Torreya nucifera, Pharbitis nil, Prunus mume, Melia azedarach, and Xanthium strumarium), and hot water (Cinnamomum cassia) herbal extracts killed the nematodes, but in varying degrees compared to the control. Only one extract was found to be promising viz Magnolia officinalis which found to be statistically different from the control and gave mean mortality of 72, 82.3, and 85.3 % for 24, 48, and 72 hours exposure, respectively. Further screening was conducted for M. officinalis with concentrations of 1,000, 100, and 10 ppm against the same species of nematode with the same time of exposure. However, it gave an excellent result for 1,000 ppm for all time of exposure, whereas for the 100 and 10 ppm it gave mean mortality of 39.5 and 25.8% for the time 72hrs, respectively that were statistically different from the control.

• Natural Product Sciences
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• 제18권1호
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• pp.47-51
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• 2012

Among 38 Vietnamese medicinal plant extracts investigated for their ${\alpha}$-glucosidase inhibitory activity, 35 extracts showed $IC_{50}$ values below $250{\mu}g/mL$. The MeOH extracts of the heartwood of Oroxylum indicum, the seeds of Caesalpinia sappan, and the fruits of Xanthium strumarium exhibited strong ${\alpha}$-glucosidase inhibitory activity with $IC_{50}$ values less than $50{\mu}g/mL$. Fractionation of the MeOH extract of the heartwood of O. indicum led to the isolation of oroxylin A (1), oroxyloside (2), hispidulin (3), apigenin (4), ficusal (5), balanophonin (6), 2- (1-hydroxymethylethyl)-4H,9H-naphtho[2,3-b]furan-4,9-dione (7), salicylic acid (8), p-hydroxybenzoic acid (9), protocatechuic acid (10), isovanillin (11), and ${\beta}$-hydroxypropiovanillon (12). Compounds 1 - 3, 5, 6, 8, 10, and 12 showed more potent activities, with $IC_{50}$ values ranging from 2.13 to $133.51{\mu}M$, than a positive control acabose ($IC_{50}$, $241.85{\mu}M$). The kinetic study indicated that oroxyloside (2) displayed mixed-type inhibition with inhibition constant (Ki) was $3.56{\mu}M$.

• 한국식품조리과학회지
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• 제13권5호
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• pp.639-647
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• 1997

The hypoglycemic effects of five Korean wild vegetables, Aralia continentalis (A. con.), Castanea crenata (C. cre.), Xanthium strumarium (K, str.), Alisma canaliculatum (A. can,) and Eupatorium chinense var. simplicifolium for tripartium (E. tri) which have been utilized for the traditional remedies were investigated in this study. Diabetes mellitus was induced in male Sprague-Dawley rats by injections of streptozotocin (STZ) into the tail veins at a dose of 45 mg/kg. Five groups of STZ-induced diabetic rats were fed one of each experimental diet containing 10% of the Korean wild vegetable powder and normal and STZ-control rats were fed the control diet for five weeks. The body weight change, feed efficiency ratio (FER) and organ weights were compared. The plasma levels of glucose, protein, cholesterol, HDL-cholesterol, triglyceride, free fatty acid, and aminotransferase activity were determined. Mineral (Ca, K, Mg, Na, Cu, Fe, Mn and Zn) contents of the Korean wild vegetables were analyzed. The body weight gain was higher in normal, C. cre., A. can. and E. tri. groups than in the diabetic control group. The FER of C. cre., A. can. and E. tri. groups was significantly higher (p.＜0.05) than that of diabetic control group. Liver weight was heavier in A. con., X. str. and A. can. groups compared with the diabetic control group. The weights of kidney were lighter in all five Korean wild vegetable groups than in the diabetic control group. After five weeks, the plasma glucose level tends to be decreased in A. con., A. can. and E. tri. groups. Plasma cholesterol level was decreased the Korean wild vegetables except for X. str. group. Plasma HDL- cholesterol level was significantly higher in A. con., A. can. and E. tri. groups compared with the diabetic control group. Plasma triglyceride and free fatty acid levels were significantly higher in X. str. group compared with the diabetic control group. Mineral contents were higher in E. tri. (Ca, K, Na and Fe). The results suggest that the intakes of A. con., A. can. and E. tri. have a hypoglycemic effect in diabetic rats showing the possibility as the valuable food resources for the prevention of diabetic mellitus.

• 동굴
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• 제56권
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• pp.15-38
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• 1998

Biomass and soil properties of plant communites was investigated in the limestone ares, Tanyang, Ch'ungbuk Province in Korea. Plants was classified in calcicoles and calcifuges within plant communities occurring in the limestone area. Biomass of plant populatins in the limestone area was measured that Stellaria aquatica was the lowest(0.58g/plant), Chrysanthemum boreale was the highest(8.87g/plant) and that Pennisetum alopecuroides, Miscanthus sinensis was 6.67g/plant, 5.76g/plant, respectively. Diameter of breast height among the tree layer communities was investigated that Pinus densiflora was the widest(20cm), Juniperus rigida was the narrowest(6cm) and that Quercus aliena, Populus ${\times}$ tomentiglandulosa was 10cm, 12cm, respectively. The high of density in the tree layer was found out Populus ${\times}$ tomentiglandulosa, Juniperus rigida, Quercus aliena, Pinus densiflora in this order. Dominant species in the study area were Pinus densiflora, Quercus aliena, Juniperus rigida, Quercus dentata in the tree layer, Lespedeza bicolor, Buxus microphylla, Rhus chinensis in the shrub layer, Humulus Japonicus, Erigeron canadensis, Xanthium strumarium, Oenothera odorata Persicaria hypropiper on the riverside, Pennisetum alopecuroides, Vicia amoena, Chrysanthemum boreale on the roadside. Soil properties of the limestone area was investigated around the protrusion of rock in the upper region, around the cave and underlayer accumulated by corrosion in the study area. Lime-chlorosis was observed in the upper region, underlayer and around the cave. It was observed that biota of kum-gul and dungbong-gul was Rhinolophus ferrumequinum korai, Epanerchodus kimi, Antrokoreana gracilipes, Diestrammena japonical. Dominant species were Humulus japonicus, Stellaria aquatica, Hydrocotyle maritima, Phragmites communis, Miscanthus sinensis around the inlet of a kum-gul. It was classified that plants in the study area was 34 order, 53 family, 135 species in all. Calcicoles were 11 order, 16 family, 18 species and calcufuges were 6 order, 8 family, 11 species of them all.

• The Korean Journal of Ecology
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• 제26권3호
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• pp.103-108
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• 2003

This study examined the coastal sand dune vegetation before and after the construction of an embankment on Anmado Island in order to compare vegetation in relation to the development of islands. A total of 24 species distributed on the sand dunes. 18 species were found to be in common before and after the construction of the embankment, which included Vitex rotundifolia, Imperata cylindrica var. koenigii, Zoysia sinica, etc. The species which were not found in this survey included Rumex japonicus, Setaria viridis, Portulaca oleracea, Artemisia japonica, Poiygonum aviculare, etc, and new species included Arena fatua, Carex boottiana, Lycium chinense, Leonurus sibiricus, Torilis japonica, Solanum carolinense, etc. The washing away of sand brought about the changes in habitat and the increase in naturalized plants, which included Oenothera odorata, Lepidium apetalum, Bidens bipinnata, Erigeron canadensis, Datura stramonium, Xanthium strumarium, Arena fatua, Solanum carolinense etc. In addition, the disturbance to this habitat led to the changes in vegetation. The main plant communities in the surveyed site were classified as Vitex rotundifolia-Imperata cylindrica var. koenigil community, Zoysia sinica-Calystegia soldanella community and Messerschmidia sibirica community. The sand dune vegetation on Anmado Island changed with regard to the community and the composition of species after the construction of the embankment, due to the sand being severely eroded. While Vitex rotundifolia community and Commelina communis community were found before the construction of the embankment, they were replaced by Vitex rotundifolia-Imperata cylindrica var. koenigii community, Zoysia sinica-Calystegia soldanella community and Messerschmidia sibirica community, after the construction of the embankment.

• 한국자원식물학회지
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• 제21권2호
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• pp.162-170
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• 2008

본 연구는 국내 습지보전 계획수립에 대한 기초자료 제공에 그 목적이 있다. 연구결과를 요약하면 다음과 같다. 안심습지에 분포하는 관속식물상은 57과 155속 179종 22변종 등 총 201 분류군이 요약되었다. 산림청 지정 희귀 및 멸종위기식물은 자라풀이었다. 습지식물의 경우 정수식물은 애기부들, 개피, 줄, 갈대, 고마리, 미나리, 물칭개나물, 침수식물은 말즘, 나사말, 검정말, 붕어마름, 물수세미로 나타났다. 그리고 부엽식물은 가래, 자라풀, 연꽃, 노랑어리연꽃, 부유식물은 생이가래, 개구리밥, 좀개구리밥으로 확인되었다. 귀화식물은 메귀리, 오리새, 소리쟁이, 묵밭소리쟁이, 털비름, 다닥냉이, 말냉이, 나도재쑥, 개소시랑개비, 벳지, 아까시나무, 족제비싸리, 토끼풀, 자주개자리, 가죽나무, 애기땅빈대, 큰달맞이꽃, 둥근잎유홍초, 둥근잎나팔꽃, 까마중, 큰개불알풀, 뚱딴지, 돼지풀, 도꼬마리, 개망초, 망초, 큰망초, 개쑥갓, 미국가막사리, 만수국아재비, 서양민들레, 붉은씨서양민들레, 방가지똥 등 총 33분류군이었다.

• 한국환경생태학회지
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• 제18권3호
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• pp.288-296
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• 2004

본 논문은 2003년 3월부터 10월까지 광주광역시 도심 대규모 하천에서 선택한 총 14개 조사 지역에서 귀화식 물과 도시화지수를 분석하였다. 조사 결과 전체 식물상은 76과 211속 273종 35변종 1품종으로 총 309종류가 확인되었다. 그 중 귀화식물은 14과 36속 44종 2변종으로 총 46종류가 확인되었다. 광주천에서는 미국개기장, 소리쟁이, 흰명아주, 토끼풀, 큰개불알풀, 돼지풀, 비짜루국화, 미국가막살이 및 서양민들레로 총 9종이 우점하였고, 황룡강에서는 소리쟁이, 토끼풀, 달맞이꽃, 돼지풀 및 망초로 총 5종이 우점하였다. 그리고 영산강에 분포하는 우점종은 광주천과 황룡강 우점종 이외에도 물참새피, 좀명아주, 다닥냉이, 개망초 및 도꼬마리 등이 나타나 총 16종이었다. 광주시 도심 하천의 평균 도시화지수는 17.2%, 귀화율은 14.9%로 확인되었다. 원산지는 유럽과 북아메리카가 각각 16종으로 가장 많았다. 도시화지수나 귀화율 같은 환경지수가 높은 도시 하천이나 둔치가 귀화식물센터 중 중요한 하나로 사료되었다.

• The Korean Journal of Ecology
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• 제24권3호
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• pp.169-180
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• 2001

대덕연구단지의 산림식생은 이차림식생과 조림식생으로 구분되었다. 이차림식생은 소나무군락(Pinus densiflora community)과 상수리나무군락(Quercus acutissima community)으로, 조림식생은 아까시나무군락(Robinia pseudoacacia community), 리기다소나무군락(Pinus rigida community), 곰솔군락(Pinus thunbergii community), 밤나무군락(Castanea crenata community)으로 구분되었다. 본 조사지역에서 출현한 종수는 총 217종이었으며, 각 군락별 평균 출현종수는 소나무군락이 21종, 상수리나무군락 20종, 아까시나무군락 20종, 리기다소나무군락 21종, 곰솔군락 21종, 밤나무군락 18종으로 밤나무군락에서 출현종이 가장 적었고, 이차림군락과 조림군락 간에는 차이가 없었다. 귀화식물은 조사구내에서 닭의장풀, 미국자리공, 개망초, 가중나무 등 12종이 출현하였고, 등산로와 산림주변부에서는 오리새, 미국가막사리, 도꼬마리, 까마중 등 19종이 출현하여 총31종의 귀화식물이 조사되었다.

• 한국자원식물학회지
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• 제23권2호
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• pp.187-196
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• 2010

본 조사는 2006년 6월 9일부터 2008년 12월 30일까지 다도해 해상국립공원지역중 여수시 금오도, 거문도, 고흥군 외나로도, 완도군 청산도, 소안도, 보길도, 진도군 하조도, 신안군 우이도, 흑산도, 홍도 지역을 중심으로 이 지역에 분포하는 귀화식물의 분포 특성을 조사하였다. 조사된 귀화식물은 총 25과 100종이 조사되었다. 고흥군 외나로도에서 82종이 조사되어 전 조사지역에서 가장 많이 분포하고, 진도군 하조도에서 68종, 흑산도와 금오도에서 각각 52, 55종으로서 비교적 많이 분포하며, 신안군 우이도에서 34종이 홍도에서 42종이 나타나, 조사지역중에서 상대적으로 귀화식물이 적게 나타났다. 전 조사지역에서 공통으로 나타난 종은 오리새, 호밀풀, 큰이삭풀, 돌소리쟁이, 흰명아주, 미국자리공, 유럽나도냉이, 다닥냉이, 콩다닥냉이, 아까시나무, 토끼풀, 덩이괭이밥, 자주괭이밥, 코스모스, 개망초, 실망초, 망초, 개쑥갓, 큰방가지똥, 방가지똥, 도꼬마리 등 26종이 나타났다. 귀화식물 분포특성을 생육지의 특성에 따라 조림지역, 해수욕장, 항구, 공한지(나대지) 주변지역, 도로경계 주변지역, 도로절개지, 비탈면 주변지역, 폐경작지 지역, 쓰레기매립장 주변지역, 습지 주변 지역 등 7개 지역으로 구분하여, 각 지역별 주요 귀화식물 분포를 비교하였다. 다도해 해상 국립공원은 국립공원내의 독특한 자연경관, 생물종, 식생경관, 생태계를 보존하기 위하여 귀화식물에 대한 체계적인 관리가 필요하다.