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Purification and Biochemical Characterization of Carboxylesterase-III from Plodia interpunctella Hubner (화랑곡나방(Plodia interpunctella Hubner) Carboxylesterase-III의 정제 및 생화학적 특성)

  • 박희윤;유종명
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.2
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    • pp.160-170
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    • 1999
  • Purification and biochemical experiments on the carboxylesterases-III (CE-III) from the indian meal moth, Plodia interpunctella (Hubner) were carried out to understand their enzymemological characteristics. The CE-III from the fifth instar larvae was purified by means of ammonium sulfate fractionation, gel permeation choromatography and ion exchange choromatography. The optimal temperature for the reaction of the CE-III on the 4 substrates ($\alpha$-Na, $\alpha$-Nb, $\beta$-Na and $\beta$-Nb) was confirmed at 4$0^{\circ}C$. The optimal pH for the reactions on the substrates $\alpha$-Na and $\alpha$-Nb was 7.5. But the optimal pH on the substrate $\beta$-Na and $\beta$-Nb was 8.0. The optimal substrate concentration for the reactions of the CE-III was 3.16 X 10$^{-3}$ M in $\alpha$-Na and $\beta$-Nb. On the substrate $\beta$-Na and $\alpha$-Nb, the optimal substrate concentration was 1.0 X 10$^{-3}$ M for CE-III. The $V_{max}$ and $K_{m}$ values of the carboxylesterases were varied by the substrates as followings: the $V_{max}$ of CE-III was 45.9 for $\alpha$-Na, 52.6 for $\beta$-Na, 36.4 for $\alpha$-Nb, and 83.3 ($\mu$ mol/min/mg protein) for $\beta$-Nb. The $K_{m}$ of CE-III was 1.43 X 10$^{-4}$ M for $\alpha$-Na, 3.57 x 10$^{-5}$ M for $\beta$-Na, 9.17 X 10$^{-5}$ M for $\alpha$-Nb, and 7.14 X 10$^{-5}$ M for $\beta$ -Nb, respectively. The CE-III seemed to have somewhat high thermostability considering that the temperature for effective denaturation on activity was about 5$0^{\circ}C$ ~ 6$0^{\circ}C$.EX>.EX>.

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X-LINKED DOMINANT HYPOPHOSPHATAEMIC RICKETS: 13 YEARS FOLLOW-UP STUDY (X 염색체 우성 저인산혈증성 구루병: 13년간의 치험례)

  • Ha, Na;Kim, Youngjin;Kim, Hyunjung;Nam, Soonhyeun
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.13 no.1
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    • pp.14-18
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    • 2017
  • X-linked dominant hypophosphataemic rickets(XLHR) is a hereditary disease generally transmitted as an X-linked dominant trait. Oral manifestations include poorly mineralized dentin, enlarged pulp chambers and root canals, early tooth loss. Also, spontaneous dental abscesses could occur in the absence of a history of trauma or caries. This report describes the clinical finding and dental treatment of a child, who came to the hospital for treatment caries but was refered to pediatrics because the child showed clinically short of status, bow-leg and radiographically enlarged pulp chamber and canal, there as diagnosed as XLHR. After the diagnosis, 13 years follow-up and treatment were performed.

Studies on changes of protein contents in various organs of the germinating soybean and of RNA contents of cotyledon of it (대두발아중(大豆發芽中)의 각기관단백질(各器官蛋白質) 및 자엽(子葉) RNA 변동(變動)에 관(關)한 연구(硏究))

  • Bae, H.W.;Yu, T.J.
    • Applied Biological Chemistry
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    • v.8
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    • pp.81-86
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    • 1967
  • Changes in the protein contents of various organs of the germinating soy bean as well as the effect of X-irradiation on the RNA content of cotyledon of it were investigated. 1) The protein content of germinating organs other than cotyledon increase while that of cotyledon decreases as germination proceeds, which is indicative of the fact that the former organs are anabolic and the latter catabolic in nature with regard to their protein metabolism during germination. 2) The cotyledon RNA content of soybean decreases until the 6 th day after germination initiated, and increases thereafter . When the seeds are exposed to $300{\gamma}$ X-irradiation, these tendencies seem to be accelerated, but when exposed to $600{\gamma}$ and $900{\gamma}$ X-irradiation the decrement of RNA in the early stage is prominent and the synthetic recovery in the later period is inhibited markedly due to radiation damage rather than radiation activation.

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Application of Early-maturing Corn to Cropping System of Forage Crop II. Effects of Late Sowing and Dense Planting of Early-maturing Corn on Forage Productivity (조숙 옥수수에 의한 사료작물 작부체계 구성 II. 조숙 옥수수의 사료생산성에 미치는 만파와 밀식효과)

  • 임근발;최영원;양종성;허운행
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.3
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    • pp.209-213
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    • 1991
  • Early maturing hybrids(Comet 80, Comet 85 and Linda) and mid-late maturing hybrid (Suwon 19) were grown at Suwon to study the influence of maturity, plant population, and sowing date (45, 55, and 65 days delayed from the recommended sowing date of Suwon 19) on the dry matter yields, percentage ears and TDN yields. Com was planted at the density of 60 x 20, 50 x 20 and 40 x 20 em. Dry matter yields were not significantly different between the early and mid-late maturing hybrids in the delayed sowing time, but significantly higher in 20 x 50cm of planting density in early maturing hybrids. As sowing was delayed to Jun. 22 ear content decreased from 40.3% (early maturing hybrid) to 6.7% (Suwon 19). This resulted in significantly 24.1% higher TDN yield in Comet 85 than in Suwon 19.

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Current Efficiency and Composit ion of Zn-Cr and Zn-Cr-X Ternary Alloy Electrodeposits (고속도금된 Zn-Cr 및 Zn-Cr-X 3원합금의 전류효율 및 조성)

  • Ye G.C.;Kim D.Y.;Ahn D.S.
    • Journal of Surface Science and Engineering
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    • v.36 no.3
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    • pp.256-262
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    • 2003
  • The current efficiency and the composition of Zn-Cr and Zn-Cr-X (X : Co, Mn) alloy electrodeposits were investigated by using chloride bath with EDTA auditive and flow cell plating system. The current efficiency of Zn-Cr alloy decreased with increasing current density, while it increased with the content of Co and Mn of the Zn-Cr-X alloy bath in high current density region. The Cr content in Zn-Cr alloy increased from 1.4-2.7 to $28wt\%$ with increasing current density and the phase structure of the alloys changed from $\eta-Zn$ through $\eta-Zn+\gamma'-ZnCr\;to\;\gamma'-ZnCr$ with Increasing Cr content of the alloys. The Co content in Zn-Cr-Co alloys increased with Co content of the bath, while Cr content of the alloy increased or decreased in low current density region $(10-75A/dm^2)$ or high current density region $(75-100A/dm^2)$, respectively. $\gamma-ZnCo$ phase was formed in the Zn-Cr-Co alloy with above $9.0wt\%$ Co. The content of Mn and Cr in Zn-Cr-Mn alloys increased or decreased with the increase of current density in high current density region, respectively while Cr content of the alloy decreased noticeably with the increase of Mn content in the bath. Two phases of $\delta_1-ZnMn$ and $\gamma'-ZnCr$ were formed in the Zn-Cr-Mn alloy with above $8.6wt\%$ Mn.

Synthesis and Magnetic Properties of Body-centered-tetragonal Fe-Co Alloy (체심정방정 구조 Fe-Co계 합금상의 합성 및 그 자기적 특성)

  • Kim, K.M.;Kwon, H.W.;Lee, J.G.;Yu, J.H.
    • Journal of the Korean Magnetics Society
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    • v.27 no.4
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    • pp.129-134
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    • 2017
  • Bulk-type body-centered-tetragonal Fe-Co alloy was synthesised by utilising a conventional alloy preparation technologies, such as melting, solidification, and homogenising treatments, and its magnetic properties were investigated. In the $(Fe_{100-x}Co_x)_{1-y}C_y$ alloy, the composition range, from which single phase body-centered-tetragonal alloy (martensite phase) was obtained, was severely limited: Co content x = 2.5, and C content y = 0.062. Tetragonality(c/a) of the synthesised body-centered-tetragonal $(Fe_{97.5}Co_{2.5})_{0.938}C_{0.062}$ alloy was 1.05. Magnetocrystalline anisotropy constant ($K_1$) of the body-centered-tetragonal $(Fe_{97.5}Co_{2.5})_{0.938}C_{0.062}$ alloy was measured to be $9.8{\times}10^5J/m^3$), which was 3.1 time as high as the pure iron (${\alpha}-Fe$).

Property of Composite Silicide from Nickel Cobalt Alloy (니켈 코발트 합금조성에 따른 복합실리사이드의 물성 연구)

  • Kim, Sang-Yeob;Song, Oh-Sung
    • Korean Journal of Materials Research
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    • v.17 no.2
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    • pp.73-80
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    • 2007
  • For the sub-65 nm CMOS process, it is necessary to develop a new silicide material and an accompanying process that allows the silicide to maintain a low sheet resistance and to have an enhanced thermal stability, thus providing for a wider process window. In this study, we have evaluated the property and unit process compatibility of newly proposed composite silicides. We fabricated composite silicide layers on single crystal silicon from $10nm-Ni_{1-x}Co_x/single-crystalline-Si(100),\;10nm-Ni_{1-x}Co_x/poly-crystalline-\;Si(100)$ wafers (x=0.2, 0.5, and 0.8) with the purpose of mimicking the silicides on source and drain actives and gates. Both the film structures were prepared by thermal evaporation and silicidized by rapid thermal annealing (RTA) from $700^{\circ}C\;to\;1100^{\circ}C$ for 40 seconds. The sheet resistance, cross-sectional microstructure, surface composition, were investigated using a four-point probe, a field emission scanning probe microscope, a field ion beam, an X-ray diffractometer, and an Auger electron depth profi1ing spectroscopy, respectively. Finally, our newly proposed composite silicides had a stable resistance up to $1100^{\circ}C$ and maintained it below $20{\Omega}/Sg$., while the conventional NiSi was limited to $700^{\circ}C$. All our results imply that the composite silicide made from NiCo alloy films may be a possible candidate for 65 nm-CMOS devices.

방사광 가속기의 광전자 분광법을 이용한 전면 발광 유기발광 다이오드에서의 열중착 산화구리와 유기물 사이의 계면 dipole 에너지 및 정공 주입 효율에 대한 연구

  • Kim, Seong-Jun;Hong, Gi-Hyeon;Kim, Gi-Su;Lee, Il-Hwan;Lee, Jong-Ram
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.03a
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    • pp.8-10
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    • 2010
  • We report the enhancement of hole injection using thermally evaporated $CuO_x$ layer between Ag anode and 4,4'-Bis[N-(1-naphthyl)-N-phenylamino]biphenyl ($\alpha$-NPD) in top-emitting organic light-emitting diode (TEOLED). The operation voltage at the current density of $1mA/cm^2$ of TEOLEDs decreased from 6.2 V to 5.0 V as the $CuO_x$ layer inserted between Ag and $\alpha$-NPD. $\alpha$-NPD was deposited in situ on Ag/$CuO_x$ and Ag anodes, and their interface dipole energies were quantitatively determined using synchrotron radiation photoemission spectroscopy. The dipole energy of Ag/$CuO_x$ was lower by 0.05 eV even though Ag/$CuO_x$ had a higher work function. The work function of Ag/$CuO_x$ is higher by 0.53 eV than that of Ag, resulting in a decrease of the turn-on voltage via reduction of hole injection barrier.

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Nest Distribution of Skuas on Barton and Weaver Peninsulas of the King George Island, the Antarctic (남극 킹조지 섬의 바톤 및 위버 반도에서 번식하는 도둑갈매기류의 둥지 분포)

  • Kim, Jeong-Hoon;Chung, Ho-Sung;Kim, Ji-Hee;Yoo, Jeong-Chil;Ahn, In-Young
    • Ocean and Polar Research
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    • v.27 no.4
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    • pp.443-450
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    • 2005
  • The ratio of breeding pairs of brown skuas (Catharacta lonnbergi), south polar skuas (C. maccormicki) and mixed species pairs between Barton and Weaver peninsulas remained the same $(X^2-test,\;X^2=0,503,\;df=2,\;p=0.778)$. The nests of skuas were clustered on Barton, whereas they were distributed randomly on the Weaver peninsula. The distance between brown skua nests, and that of mixed species pair nests were longer than those of south polar skua nests. Brown skua nests were distributed along the coast. Whereas, the nests of mixed species pairs and south polar skuas were found more frequently inland (Kruskal-Wallis, $X^2=11.631$, df=2, p<0.005). There was no interspecific difference in the distances between skua nests and Penguin rookery at Barton (Kruskal-Wallis, $X^2=2.153$, df=2, p=0.341) or at King Sejong Station (ANOVA, F=1.483, df=2, p=0.229). In general, skuas prefer lower locales (<125m above sea level) for their nest building sites. Brown skua nests were distributed mainly on the beach, whereas south polar skua were distributed on the predominant periglacial landforms and till areas $(X^2-test,\;X^2=24.988,\;df=8,\;p<0.005)$.

Cytotaxonomical Study of the Chenopodium album and its Related Species in Korea (한국산 흰명아주와 근연종의 세포분류학적 연구)

  • Chung, Youngjae;l Kim, Muyeol;Lee, Byongsoon
    • Korean Journal of Plant Taxonomy
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    • v.41 no.4
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    • pp.324-328
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    • 2011
  • The purpose of this study was to analyze the interspecific relationships of Chenopodium album and its related taxa collected in Korea. The 18S-26S ribosomal DNA (45S rDNA) loci were detected directly on mitotic chromosomes by fluorescence in situ hybridization (FISH) and the chromosome numbers were examined using aceto-orcein methods. The chromosomal numbers of Chenopodium album var. album and C. album var. centrorubrum were 2n = 6x = 54, whereas for C. album var. stenophyllum, this number was 2n = 4x = 36. The basic chromosome number was x = 9. The biotin labeled 18S-26S rDNA probe exhibited eight yellow fluorescent signals on the metaphase chromosome of C. album var. album and var. centrorubrum respectively, while two yellow signals of C. album var. stenophyllum were noted. All of the signals on the chromosomes were located at the terminal regions. The chromosome number and FISH findings suggest that C. album var. centrorubrum is merged into var. album and that it is clearly distinguished from C. album var. stenophyllum.