• Journal of Periodontal and Implant Science
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• 제39권1호
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• pp.45-52
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• 2009

Purpose: Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells. Materials and methods: PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed. Results: PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes. Conclusion: These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.

• Journal of Periodontal and Implant Science
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• 제52권1호
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• pp.65-76
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• 2022

Purpose: The present study measured changes in arteriolar and venular capillary flow and structure in the gingival tissues during the development of plaque-induced gingival inflammation by combining dynamic optical coherence tomography (OCT), laser perfusion, and capillaroscopic video imaging. Methods: Gingival inflammation was induced in 21 healthy volunteers over a 3-week period. Gingival blood flow and capillary morphology were measured by dynamic OCT, laser perfusion imaging, and capillaroscopy, including a baseline assessment of capillary glycocalyx thickness. Venular capillary flow was estimated by analysis of the perfusion images and mean blood velocity/acceleration in the capillaroscopic images. Readings were recorded at baseline and weekly over the 3 weeks of plaque accumulation and 2 weeks after brushing was resumed. Results: Perfusion imaging demonstrated a significant reduction of gingival blood flow after 1 and 2 weeks of plaque accumulation (P<0.05), but by 3 weeks of plaque accumulation there was a more mixed picture, with reduced flow in some participants and increased flow in others. Participants with reduced flux at 3 weeks also demonstrated venular-type flow as determined by perfusion images and evidence of the development of venular capillaries as assessed by the velocity/acceleration ratio in capillaroscopic images. After brushing resumed, these venular capillaries were broken down and replaced by arteriolar capillaries. Conclusions: After 3 weeks of plaque accumulation, there was wide variation in microvascular reactions between the participants. Reduced capillary flow was associated with the development of venular capillaries in some individuals. This is noteworthy, as an early increase in venous capillaries is a key vascular feature of cardiovascular disease, psoriasis, Sjögren syndrome, and rheumatoid arthritis-diseases with a significant association with the development of severe gingival inflammation, which leads to periodontitis. Future investigations of microvascular changes in gingival inflammation might benefit from accurate capillary flow velocity measurements to assess the development of venular capillaries.

• International Journal of Computer Science & Network Security
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• 제22권10호
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• pp.97-106
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• 2022

Objective. Nanotechnology is spreading among all areas of life, from everyday devices to medicine. The concept of nanotechnology argues that not only can new physical and chemical properties of materials be discovered, but also the new potential of nanostructures when reduced to the nanoscale. The growing interest in the application of nanomaterials in dentistry contributes to the proliferation of the range of nanomaterials used by specialists. The purpose of this review of information sources was to analyze the prospects for the use of nanomaterials in dentistry. Methods. We used the bibliographic semantic method of research, for which we analyzed electronic databases of primary literature sources Scopus, Web of Science, Research Gate, PubMed, MDPI, and MedLine. English-language scientific articles published after 2017 were taken into consideration. Results. According to the results of a search study among modern information primary sources, nanotechnology improves the preventive properties of oral care products, improves the structural-mechanical and aesthetic properties of composite mixtures for dentistry, overcomes the problems of the clinical application of dental implants. Despite the prospects of nanotechnology applications in medicine in general and dentistry in particular, the existing economic and technological problems require a thorough solution for further implementation of nanostructures. Scientific novelty. For the first time, the analysis of modern trends in the application of nanotechnology in dentistry is carried out and the peculiarities of materials are highlighted, the problems and prospects of nanostructures implementation in modern dental implantology are given, physical, chemical, mechanical, and antibacterial properties of nanomaterials are evaluated. The effect of nanomaterials on the microbial adhesion of the tooth or implant surface is described. Practical significance. The presented publication can become a scientific basis for the solution of urgent problems hindering the introduction of nanotechnology into dental practice. Conclusions. Thus, the use of nanostructures opens up great opportunities for the treatment of a wide range of diseases, not only of dental nature but also in medicine in general.

• 대한치과교정학회지
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• 제38권6호
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• pp.397-406
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• 2008

교정용 미니 임플랜트의 식립 부위에 대한 연구는 주로 구치부 치근사이 공간에 집중되어 왔다. 본 연구의 목적은 전치에서 구치에 이르는 치아간의 피질골 두께와 치근간 거리를 측정함으로써 교정용 미니 임플랜트 식립 시에 참고 할 수 있는 임상적 지침을 제공하는 것이다 연구를 위해 성인 28명(남자 14명, 여자 14명)의 CT를 V-works $4.0^{TM}$을 이용하여 3차원 영상으로 전환하였다. 중절치에서 제2대구치에 이르는 모든 치아 사이를 치간 접촉점을 지나면서 교합 평면에 수직이 되도록 잘라 $90^{\circ}$ 단면을 형성한 후 치조정으로부터 높이를 달리하여 0, 15, 30, $45^{\circ}$의 각도를 주어 피질골의 두께를 측정하였다. 또한 치조정으로부터 2, 4, 6 mm 높이에서 교합 평면에 평행하게 잘라 $90^{\circ}$ 단면을 만든 후 치근간 거리를 측정하였다. 피질골의 두께는 전 치부에서 구치부로 갈수록 두꺼워지는 경향을 보였으며, 5-6과 1-1 사이, 6-7과 1-1, 1-2, 2-3 사이에서는 유의한 차이를 보였다 (p < 0.05). 치조정으로부터 2 mm 높이를 제외한 대부분의 위치에서 각도가 증가함에 따라 피질골의 두께가 급격히 증가하는 경향을 보였고, 4 - 6 mm 높이에 식립시 $30-45^{\circ}$ 이상의 각도를 부여해야 피질골 보유량(engage 양)에 유의한 차이를 보였다. 치근간 거리 측정 결과 4-5, 5-6, 6-7 사이가 치근 손상 없이 미니 임플랜트를 식립하기에 적절한 위치라고 볼 수 있었고, 1-1과 1-2 사이는 미니 임플랜트 식립을 위한 충분한 치근간 거리를 제공하지 못하는 것으로 나타났다. 본 실험의 결과로 볼 때 피질골과 미니임플랜트의 접촉면을 증가시키기 위해서는 치조정에서 치근단부로 4와 6 mm 되는 부위에서 $30^{\circ}$ 또는 $45^{\circ}$로 식립하는 것이 유리할 것으로 보인다.

• 대한정형외과학회지
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• 제55권3호
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• pp.244-252
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• 2020

목적: 대퇴골 전치환술은 사지재건술의 극단적 술식 중 하나이며 증례가 드물어 이에 대한 연구는 주로 술식 후의 합병증에 대한 분석이며 본 술식의 적응증에 대한 분석은 미미하다. 저자들은 대퇴골 전치환술 36예의 1) 종양적 문제로 치환술을 받은 환자의 생존에 관련된 예후인자, 2) 치환물 및 하지의 생존율, 3) 치환물을 3년 이상 추시한 예의 합병증, 최종 하지 상태 및 기능적 결과를 알아보고자 하였다. 대상 및 방법: 대퇴골 전치환술을 받은 이유에 따라 원발성 종양에 의한 경우(15예, 1군), 오인 수술 및 국소재발로 인한 경우(16예, 2군), 재건물 실패로 인한 경우(5예, 3군)의 세 군으로 분류하였다. 환자 생존인자 분석 항목은 나이, 성별, 전치환술 원인, 항암요법 전후 종양 부피 변화, 절제연, 국소재발이었다. 결과: 전체 36예의 5년 환자 생존율은 31.5%±16.2%였다. 종양과 관련된 적응증으로 치환술을 받은 31예의 5년 생존율은 21.1%±15.6%였다. 항암요법 전후 종양의 크기가 같거나 감소한 10예의 5년 생존율은 50.0%±31.0%로 크기가 증가한 13예의 0.0%±0.0%보다 유의하게 높았다(p=0.02). 광범위 절제연을 얻은 12예의 5년 생존율은 41.7%±27.9%로 변연부 절제연의 0.0%±0.0% 보다 유의하게 높았다(p=0.03). 성별, 나이, 대퇴골 전치환술을 시행받은 원인, 치환술 후 국소 재발여부와 생존율과의 유의한 차이는 없었다. 치환물 36예에서 5년 생존율은 16.6%±18.2%였다. 하지의 10년 생존율은 85.9%±14.1%였다. 최종 추시상 종양인 공관절을 유지한 것이 12예, 회전성형술은 2예, 슬관절 상부 절단술 1예, 관절고정술 1예였다. 대퇴골 전치환술 후 3년 이상 추시가 가능했던 16예 중 수술이 필요했던 주 합병증이 14예, 보존적 치료만 했던 저등급 감염이 2예였다. 수술한 환자 14예 중 감염이 10예(71.4%)예, 국소재발이 2예, 고관절 탈구, bushing 파손, 대퇴동맥 폐색이 각각 1예였다. 결론: 종양과 관련된 적응증으로 대퇴골 전치환술을 시행한 환자 중 항암요법 후 종양의 크기가 커지거나 절제연이 불량한 경우, 환자가 조기에 사망하는 경우가 많았다. 따라서 전치환술의 시행에 신중해야 할 것으로 생각된다. 장기 생존 환자에서도 감염률이 높고 기능적 결과도 만족스럽지 않으나 현재 적응증을 고려했을 때 대퇴골 전치환술은 사지보존을 위한 최선의 방법으로 보인다.

• Journal of Periodontal and Implant Science
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• 제33권3호
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• pp.519-531
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• 2003

The aim of present investigation was to clinically measure the thickness of palatal masticatory mucosa in the hard palate as potential donor site for mucogingival surgery, to determine the relation to shape of palatal vault, form of tooth, gender, and to serve the clinical criteria t o choose the proper surgical technique. 84(mean age:25yrs) systemically and periodontally healthy volunteers participated in this study and 18 standard measurement points were defined in the hard palate, located on 3 lies which ran at different distances parallel to the gingival margin. 6 positions were designated on each of these 3 lines between the level of canine and 2nd molar and a bone sounding technique using a periodontal probe with minimal local anesthesia was utilized to assess the thickness of palatal masticatory mucosa. Student t-test was used to determine the difference in mucosal thickness between 2 groups gender, shape of palatal vault (high palatal vault vs. low palatal vault), tooth form (short-wide vs. long narrow) The result of this study were as follows: 1. Soft tissue thickness progressively increased in sites further away from the gingival margin (p<0.01). 2. Depending on position, in line a and line c the masticatory mucosal thickness increased from Ca to M2(p<0.01), but in line b the thickness increased from Ca to P2, and decreased to M1 and increased again to M2. 3. Gender did not influence the thickness of masticatory mucosa. 4. Palatal vault shape was associated with the thickness of masticatory mucosa. Thickness of low palatal vault group was thicker than high palatal group between P2 and M2 position. 5. Form of tooth did not influence the thickness of masticatory mucosa. In conclusion, palatal vault shape was associated with the thickness of masticatory mucosa. So, mucogingival surgery can be considered as a treatment modality in high palatal vault group. But, Gender and tooth form did not influence the thickness of masticatory mucosa.

• Journal of Periodontal and Implant Science
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• 제30권3호
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• pp.661-676
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• 2000

Gingivitis and periodontitis are infectious diseases in that microorganisms are the primary extrinsic cause of the diseases. the occurrence of gingivitis has been associated clearly with the presence of microorganisms at the disease site, and the histologic nature of the tissue involved is indicative of an inflammatory response induced by microorganisms. additional evidence for the microbial etiology of periodontal disease is that numerous antimicrobial agents are effective in reducing plaque accumulation and periodontal diseases. the purpose of this article is to analyze the antimicrobial effects of Pulsatilla koreana. Well-dried Pulsatilla koreana purchased from herbs distributor was ground and extracted into methanol(MeOH), ethylacetate(EtoAc), chlorform($CHCl_3$) and Butyl alcohol(BuOH). we have then applied each solution to the bacteria samples(Bacteroides forsythus, Streptococcus mutans, Streptococcus sanguis, Porphylomonas gingivalis, Actinobacillus actinomycetemcomitans, Eikenella corrodens, Prevotella intermedia, Actinomyces viscosus, Prevotella nigrescens , Rothia dentocariosa, Fusobacterium nucleatum, Pseudomonas aeruginosa, Staphylococcus aureus) collected from several organizations. To conduct susceptibility test(Kirby-Bauer method), plate contained each periodontopathic bacteria is spread extracted into methanol(MeOH), ethylacetate(EtoAc), chlorform($CHCl_3$) and Butyl alcohol(BuOH) and to measure the minimum inhibition concentration(MIC) of the bacteria against the solutions to ultimately determine antimicrobial effects of the solutions, insert bacteria sample into $20{\mu\ell}/{m\ell}$, $10{\mu\ell}/{m\ell}$, $5{\mu\ell}/{m\ell}$, $2.5{\mu\ell}/{m\ell}$ of each solution and control group(not contained solution) 1. Solution extracted into methanol did not show clear zone against all bacteria samples. Only P.nigrescens, S. mutans and S. sanguis in solution extracted into ethylacetate, S. mutans and S. anguis in solutions extracted into chlorform and Butyl alcohol showed clear zone against all bacteria samples. Solution extracted into Butyl alcohol showed clear zone against 13 types of bacteria, excluding P. gingivalis. 2. In Solution extracted into methanol, the bacteria samples grew in the highest concentrated plate, showing minimal variation from control group. 3. In Solution extracted into Butyl alcohol, S. aureus, P. intermedia, E. corrodens, A. actinomycetemcomitans, B. forsythus, P. gingivalis et al. showed decreased growth in the highest concentrated plate. P. auruginosa, R. dentocariosa, A. viscosus, P. nigrescens, S. mutans et al. showed decreased growth at MIC $20{\mu\ell}/{m\ell}$ and S. sanguis showed decreased growth at MIC $10{\mu\ell}/{m\ell}$. 4. By analyzing the MIC level through considering the results from Kirby-Bauer method, Solution extracted into methanol did not reveal any antimicrobial effects and Solution extracted into Butyl alcohol showed the highest antimicrobial effects In conclusion, it can be used the extracts of Pulsatilla koreana as wide spectrum antimicrobial agent.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2016년도 추계학술대회 논문집
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• pp.197-197
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• 2016

Commercially pure titanium (CP Ti) and Ti-6Al-4V alloys have been widely used for biomedical applications. However, the use of the Ti-6Al-4V alloy in biomaterial is then a subject of controversy because aluminum ions and vanadium oxide have potential detrimental influence on the human body due to vanadium and aluminum. Hence, recent works showed that the synthesis of new Ti-based alloys for implant application involves more biocompatible metallic alloying element, such as, Nb, Hf, Zr and Mo. In particular, Nb and Hf are one of the most effective Ti ${\beta}-stabilizer$ and reducing the elastic modulus. Plasma electrolyte oxidation (PEO) is known as excellent method in the biocompatibility of biomaterial due to quickly coating time and controlled coating condition. The anodized oxide layer and diameter modulation of Ti alloys can be obtained function of improvement of cell adhesion. Manganese(Mn) plays very important roles in essential for normal growth and metabolism of skeletal tissue in vertebrates and can be detected as minor constituents in teeth and bone. Radio frequency(RF) magnetron sputtering in the various PVD methods has high deposition rates, high-purity films, extremely high adhesion of films, and excellent uniform layers for depositing a wide range of materials, including metals, alloys and ceramics like a hydroxyapatite. The aim of this study is to research the Mn coatings on the micro-pore formed Ti-29Nb-xHf alloys by RF-magnetron sputtering for dental applications. Ti-29Nb-xHf (x= 0, 3, 7 and 15wt%, mass fraction) alloys were prepared Ti-29Nb-xHf alloys of containing Hf up from 0 wt% to 15 wt% were melted by using a vacuum furnace. Ti-29Nb-xHf alloys were homogenized for 2 hr at $1050^{\circ}C$. Each alloy was anodized in solution containing typically 0.15 M calcium acetate monohydrate + 0.02 M calcium glycerophosphate at room temperature. A direct current power source was used for the process of anodization. Anodized alloys was prepared using 270V~300V anodization voltage at room. Mn coatings was produced by RF-magnetron sputtering system. RF power of 100W was applied to the target for 1h at room temperature. The microstructure, phase and composition of Mn coated oxide surface of Ti-29Nb-xHf alloys were examined by FE-SEM, EDS, and XRD.

• 대한가정학회지
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• 제17권4호
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• pp.117-127
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• 1979

This study attempts to shed additional light on the national diffusion process and interaction of welfare services for children and mothers. The central hypothesis examined is the following : In general, the implant of social welfare system is first considered on the characteristics of their own socio-economics , and diffusion prodess of welfare services tends to occur faster in countries with open sicieties than in countries where entry barriers are high and society ranking rigid. The scope of the study is limited in several ways. Attention is concentrated on the agency of woman in the belief that an in-depth case study of one field can yield more insight than a less detailed investigation embracing a number of social welfare systems. The angency of woman is selected for several reasons. a) It is important the agency of woman alone produce more effective than services affecting both the child welfare and women's libertion bradually. Moreover, these services will form the heart of mother's welfare. b) The services of women's agency is gradually wide spread, since a role of government is still negligible before a full realization of economic income. c) The services of women agency is a prime example of a social welfare. d) statistical data and other information are more readily available. One of major findings of our study is the existence of the barriers inhibiting child welfare form " motherhood cult". Despite all the distinct advantages for mother and child , social attitudes appear to be some resistant on child welfare system among men and women even the agenices of women. What is surprised is the extent of the awareness that strategies of women's agency to liberate woman and to increate activities of women involve the strategies to liberate child. The conviction that solving application problems for child welfare in line with the activities of agency by women offer greater freedom and more advantage to mother are not apparent on the data collected from 18 of multi-functional agencies in our country. there are little sign that such agencies cooperate toward attainment of common goals. The intensive early focus of the movement on consciousness raising is a necessary beginning and such efforts by women's organizations would help both in the continuous evolution of women and child. Some of the agencies offer a variety of services to women and children, others are specialized in that they offer fewer services or even only one. Social welfare agenices giving service to children and their families are identified by various names usually women service and child welfare from govern , mental agency and voluntary public agency to foreign aided. What is totally missing , however, is an integrated synthesis of child welfare program Through women's agency. Social changes to occur, but delineation of appropriate and comprehensive social policy is also necessary at this point at this point since efforts, social changes and polices must be well coordianted and focused, as well as mutually supprotive and reinforcing.

• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.83-91
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• 2004

2-piece 임플란트에서는 초기 치유 기간 동안 0.9-1.6mm의 골 소실이 일어나는데 2-piece 임플란트의 미세 간극과 그에 따른 생물학적 폭경의 형성이 중요한 원인이다. 최근 수직적으로 미세 간극의 위치를 변화시킴으로 골 소실의 양을 줄일 수 있다는 보고가 있다. 이번 실험의 목적은 미세 간극의 수평적 위치 변화에 따른 골 소실의 양을 비교하는데 있다. 하악에서 인접하여 최소 2개의 임플란트를 식립할 수 있는 7 무치악 부위에 총 15개의 Osseotitie XP 4/5를 식립하였다. 이때 임플란트의 상연이 주위 치조골과 일치하게 식립하였고 무작위로 선택하여 한 그룹(W군)에서는 wide diameter healing abutment를 연결하였고 다른 한 그룹(S 군)에서는 standard diameter healing abutment를 연결하였다. 3개월의 치유 기간후 보철 과정을 시작하였으며 이 때 healing abutment와 같은 크기의 prosthetic component를 이용하였다. 임플란트 식립 직후, 3개월의 치유 기간이 지난 보철 직전(Interval I)에, 보철 과정 직후(Interval II)에 각각 치근단 방사선 사진을 찍어 각 단계에서의 골 소실 양을 비교하였다. W 군의 경우 골 소실의 양이 Interval I에서 $1.60{\pm}0.78$, Interval I+II에서 $2.36{\pm}0.29$이었고 S 군에서는 Interval I에서 $1.5810{\pm}0.3030$, Interval I+II에서는 $1.7346{\pm}0.4199$이었다. W군에서는 Interval I와 I+II에서의 골 소실 양이 통계학적으로 유의할 만한 차이를 보였으며, Interval I+II에서의 W 군과 S 군에서의 골 소실 양도 통계학적으로 유의할 만한 차이를 나타내었다. Interval I에서는 두 그룹에서 골 소실의 차이가 없었는데 이는 1 stage surgery시 healing abutment 주위로 mucoperiosteal flap 접합의 어려움 때문으로 생각된다. 한편 Interval II에서는 abutment manipulation 등의 과정이 추가적인 골 소실을 야기한 것으로 생각된다. Interval I+II에서 W 군과 S 군 사이의 골 소실 양 차이는 미세 간극의 수평적 위치 변화의 양과 유사한 결과를 나타내었는데 이로 미루어 미세 간극의 수평적 이동은 임플란트 주위의 골 소실 양에 영향을 미칠 수 있다고 생각된다.