• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.307-312
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• 2000

This study was carried out to develop an automatic grafting system suitable for fruit-vegetable seedlings. The study consisted of two research sections: 1) development of a medium-sized, low-cost automatic grafting system for cooperative farms and commercial seedlings production company, and 2) commercializing research for prototype development based on the above concepts. The grafting robot developed in this research can be described as follows, a. Developed grafting robot can cover the whole operations for grafting scion and rootstock, only if operator provides scion and rootstock tray. b. Five seedlings can be grafted at one time, and about 1,200 seedlings can be grafted in one hour. c. The success ratio of mechanical grafting scion and rootstock with ceramic pin bonding provided by the holder is more than 95% when the conditions of seedlings are satisfied. d. The grafting efficiency has improved over 10 times compared with manual work, and the grafting cost generated 44% savings.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.1-11
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• 2016

Single nucleotide polymorphism (SNP) is an abundant form of genetic variation within individuals of species. DNA polymorphism can arise throughout the whole genome at different frequencies in different species. SNP may cause phenotypic diversity among individuals, such as individuals with different color of plants or fruits, fruit size, ripening, flowering time adaptation, quality of crops, grain yields, or tolerance to various abiotic and biotic factors. SNP may result in changes in amino acids in the exon of a gene (asynonymous). SNP can also be silent (present in coding region but synonymous). It may simply occur in the noncoding regions without having any effect. SNP may influence the promoter activity for gene expression and finally produce functional protein through transcription. Therefore, the identification of functional SNP in genes and analysis of their effects on phenotype may lead to better understanding of their impact on gene function for varietal improvement. In this mini-review, we focused on evidences revealing the role of functional SNPs in genes and their phenotypic effects for the purpose of crop improvements.

• The Korean Journal of Food And Nutrition
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• v.33 no.3
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• pp.223-236
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• 2020

In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.152-155
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• 2000

The contents of various capsaicinoids viz. nordihydrocapsaicin (NDC), capsaicin (CAP), and dihydrocapsaicin (DHC) were determined in different parts of fruits (placenta, pericarps, seeds, and whole fruits) at different developmental stages after flowering and compared with the capsaicinoid synthetase (CS) activity. The capsaicinoid contents were very low up to 24 days after flowering (DAF), and there was a significant increase at 36 DAF in all parts of fruits. The enzyme activity of placenta increased to maximum at 24 DAF, and thereafter it gradually decreased. There were no significant amounts of enzyme activities in other parts of the fruits. In Subicho (inbred line) the content of DHC was slightly higher than CAP in all parts. of the fruits throughout the development stages of fruits, whereas in Chung Yang the CAP content was higher compared to the DHC content. The contents of total capsaicinoids in Chung Yang were also higher than Subicho. However, the crude enzyme extract obtained from Chung Yang led to the synthesis of DHC almost exclusively when the substrate, 8-methyl nonanoic acid, was added to the reaction mixture. Our results suggest that the composition of individual analogue of capsaicinoids depends upon the substrate available in the fruits.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.164-169
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• 2004

A spatial interpolation scheme incorporating local geographic potential for cold air accumulation (TOPSIM) was used to test the feasibility of operational frost warning in Chatancheon basin in Yeoncheon County, where the introduction of new crops including temperate zone fruits is planned. Air temperature from April to June 2003 was measured at one-minute intervals at four locations within the basin. Cold-air accumulation potentials (CAP) at 4 sites were calculated for 3 different catchment scales: a rectangular area of 65 x 55 km which covers the whole county, the KOWACO (Korea Water Corporation) hydrologic unit which includes all 4 sites, and the sub-basins delineated by a stream network analysis of the digital elevation model. Daily minimum temperatures at 4 sites were calculated by interpolating the perfect prognosis (i.e., synoptic observations at KMA Dongducheon station) based on TOPSIM with 3 different CAPs. Mean error, mean absolute error, and root mean square error were calculated for 45 days with no precipitation to test the model performance. For the 3 flat locations, little difference was detected in model performance among 3 catchment areas, but the best performance was found with the CAPs calculated for sub-basins at one site (Oksan) on complex terrain. When TOPSIM loaded with sub-basin CAPs was applied to Oksan to predict frost events during the fruit flowering period in 2004, the goodness of fit was sufficient for making an operational frost warning system for mountainous areas.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.78-81
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• 2011

This study compares and contrasts the Wales and England Organic Action Plans (WOAP and EOAP) and a Korean regional Life-Food Development Plan (KLFDP) in order to facilitate the development of the organic sector in Korea. Early action plans, for example, the first WOAP (1999) focused support on developing the supply of organic products whereas later action plans focused on marketing and consumer. OAPs may not only provide specific issue-solving roles by proposing new policy measures but also perform a regulatory role as a controller for organic sector development as a whole. The current KLFDP seems to stick to the former role but hardly has the latter role such as setting priorities, harmonizing various conflicting policy measures and factors and performing evaluation process for further progress. To secure better harmonized and sustainable development of Korean organic farming sector, constructing comprehensive national-level organic action plan, which has policy developing, implementing, regulating, evaluating and evolving functions, might be the most efficient choice.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.529-533
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• 2006

To produce fresh-cut fruit products of high quality, the combined effects of postharvest calcium and heat treatment on Tsugaru apple were examined. Whole fruits were treated for 20 min at three different conditions: dipping in 3% $CaCl_2$ solution at $45^{\circ}C$ or ambient temperature, or in heated water at $45^{\circ}C$. The calcium content of the apples dipped in $CaCl_2$ solution at $45^{\circ}C$ was higher than that of the control and the non-heated calcium dipping. The fruits with calcium and heat treatment showed the same pattern of respiration rates as the control did during storage. The browning degree of the apple slices increased to approximately 3% after one-day storage, but no significant difference was observed between the treated and untreated apples. The fruits with $CaCl_2$ solution dipping at $45^{\circ}C$ had higher firmness than those with calcium dipping or heat treatment alone.

• Journal of Nutrition and Health
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• v.28 no.1
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• pp.23-32
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• 1995

This study was performed to investigate the influence of dietary fibers from the whole foods on the serum lipid level and bowel function in rats. The fiber sources of experimental diets were prepared by drying and milling of cereal(rice bran), vegetables(Korean cabbage, radish), fruit(apple), and sea weeds(laver, sea tangle). Each of fiber sources was mixed into the diet to make the 5% level of total dietary fiber. Male rats of Sprague-Dawley strain were blocked into 8 groups : FF, CC, CE, V1, V2, ER, S1, and S2. The animals were fed ad libitum each of experimental diets for 4 weeks. Serum triglyceride level was not significantly different among groups. The laver group showed the lowest level in the serum total cholesterol. The Korean cabbage group showed the highest level in the serum HDL cholesterol. The longest transit time was observed in the fiber free group and the shortest transit time was observed in the sea tangle group. Absorption rates of calcium were especially lower in the Korean cabbage and the sea tangle groups than the other groups. Magnesium and phosphorus absorption rates were influenced by SDF(Soluble Dietary Fiber) intake and TDF(Total Dietary Fiber) intake, respectively. Mucosa weight in the small intestine showed the tendency to increase by increasing of IDF (Insoluble Dietary Fiber) intake. especially the Korean cabbage group was the heaviest. The activity of maltase in the mucosa of small intestine was the lowest in the radish group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.2
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• pp.222-229
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• 2016

Corni fructus is often distributed or processed in the form of dried fruit. However, Corni fructus is hard to develop due to its distinctive sour, bitter, and astringent taste. The aim of this study was to develop a puree to broaden the utilization of fresh Corni fructus. Manufacturing and quality characteristics of Sansuyu jam made from puree were investigated. Seeded Corni fructus pulp consisted of 20 to 26% whole fruit. The moisture and sugar contents of pulp were 52~63% and $15{\sim}31^{\circ}Brix$, respectively. Sterilized distilled water was added to seeded pulp to achieve a constant solids content in the puree. As the pectin content was low as $0.14{\pm}0.01%$, gelling agent was added to produce jam. The moisture content of the puree increased to 83~88%. The sugar content was reduced to $10^{\circ}Brix$. There was no significant difference in pH. DPPH radical scavenging activities of the puree according to ripening rate at a concentration of 100 ppm were 47.92% and 50.96%, respectively. The preference degree was $5.03{\pm}0.97$ at a ripening ratio of 50:50, 2% pectin, and 0.2% carrageenan. These results imply that Corni fructus pulp puree may be appropriate for development as a natural food product.

• Journal of the Korean Dietetic Association
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• v.13 no.1
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• pp.61-72
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• 2007

In order to study milk preference and intake pattern of adolescents, the questionnaire survey was conducted with 929 middle and high school students residing in Chungnam cities through October and November 2004. Of the subjects, 56.3% liked milk or liked very much. They preferred chocolate and fruit-tasted milk to plain milk. Reasons for liking milk were for health and its taste, while the reasons of disliking were unpleasant smell and stomach upset. However those who disliked milk did like milk products such as ice-cream, yogurt or cheese. Of the students 35.1% drank milk 2 times or more a day, while 11.8% drank milk less than 2-3 times a month. As a whole they drank milk 8.6$\pm$6.7 times a week, of which 4.3$\pm$3.1 times for plain milk. Daily intake amount was estimated as 308$\pm$315mL. Middle-school students and boys drank more milk than high-school students and girls, respectively. Among the students 64.3% used to drink 1 cup of milk at a time, and 58.3% drank only milk without other foods. It was shown that 51.8% took milk school-serviced and their milk intake frequencies were higher than those of who were not given school milk service. Also milk intake frequencies was higher in students whose mother were housewives than in students whose mother had jobs. Milk preference, intake frequency, and one portion size showed significant and positive correlations each other. In conclusion, milk intake level of the subjects was insufficient and unpleasant smell and stomach upset were the main reasons which decreased milk preference and consumption. Students with low milk preference should be guided with how to drink milk deliciously, how to choose milk type and its products. It is also effective to go into school milk service and to provide milk at home anytime. As students like much fruit-tasted and chocolate milk, the milk processing industries should take their nutritional and health benefits into considerations importantly.