• 한국약용작물학회지
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• 제19권5호
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• pp.373-379
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• 2011

Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.

• 대한한방소아과학회지
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• 제29권1호
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• pp.1-14
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• 2015

Objectives This experimental study was designed to investigate the effects of Dai-saiko-to (DSH) on differentiation of 3T3-L1 preadipocytes and body weight, serum lipid levels in high-fat diet-induced obese mice. Materials and Methods Cells were incubated with DSH at an indicated concentration (0.01-1 mg/ml) for 24h, then the growth rate was assessed by MTS assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of DSH. On Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) and cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancer-binding proteins ${\alpha}$ ($C/EBP{\alpha}$) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. Four-weeks old mice (wild-type C57BL/6) were used for all experiments. Body weight gain and serum lipid levels were measured in the obesity-induced mice. Results DSH did not show toxicity even at the concentration of 1 mg/ml and DSH significantly inhibited the differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. Also, DSH significantly reduced the expressions of $PPAR{\gamma}$ and $C/EBP{\alpha}$ in a dose-dependent manner. Furthermore, DSH significantly reduced body weight gain, serum glucose, total cholesterol and LDL-cholesterol contents in obesity-induced mice. Conclusions These results demonstrated that DSH inhibited 3T3-L1 preadipocyte differentiations and high-fat diet-induced obesity in mice.

• 생약학회지
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• 제45권1호
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• pp.28-34
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• 2014

Rhei Rhizoma (RR) and Moutan cortex (MC) have been reported to have anti-inflammatory effects. However, little is known about the effects of RR and MC on endothelial inflammation and insulin resistance (IR). This study aims to investigate whether the water extracts of RR and MC could exert protection against palmitic acid (PA)-induced inflammation and IR in human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated for 6 h with RR or MC, and then exposed to PA for 24 h. The levels of interleukin-6 (IL-6) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were determined by enzyme-linked immunosorbant assay kits. Western blot analysis was performed for activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and insulin receptor substrate-1 (IRS-1). In HUVECs stimulated with PA, both RR and MC significantly inhibited the production of TNF-${\alpha}$ and IL-6 and the activation of NF-${\kappa}B$. At the same concentrations, the inhibitory effects of RR were more potent than those of MC. PA reduced insulin-induced phosphorylation of IRS-1, which was reversed by RR and MC. The results suggest that RR and MC are effective in inhibiting PA-associated endothelial inflammation and ameliorating IR by beneficial regulation of NF-${\kappa}B$ and IRS-1 activation.

• 한방재활의학과학회지
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• 제19권2호
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• pp.103-112
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• 2009

Objectives : This study was to evaluate the pharmacological effect of Korea Red Ginseng aqueous extract (KRGE) on serum-deprived apoptosis of neuronal-like pheochromocytoma PC12 cells and to investigate its underlying action mechanism. Methods : KRGE was prepared by extracting Korea Red Ginseng with hot water and concentrating using a vacuum evaporator. Cell viability was determined after incubation of cells with KRGE or chemical inhibitor in serum-deprived medium for 60 h by counting intact nuclei following lysing of the cell membrane. Caspase activities were measured using chromogenic substrates and signal-associated protein phosphorylation and cytochrome c release were determined by Western blot analyses using their specific antibodies. Results : Serum deprivation induced PC12 cell death, which was accompanied by typical morphological features of apoptotic cell, such as nuclear fragmentation, caspase-3 activation, and cytochrome c release. This apoptotic cell death was significantly inhibited by KRGE and caspase-3 inhibitor, but not by the addition of NMA, ODQ, and PD98059. KRGE promoted phosphorylation of Akt and Bad, and this phosphorylation was inhibited by the PI3K inhibitor LY92004. In addition, this inhibitor also reversed KRGE-mediated protection of PC 12 cells from serum deprivation. These results suggested that KRGE protects PC12 cells from serum deprivation-induced apoptosis through the activation of PI3K/Akt-dependent Bad phosphorylation and cytochrome c release, resulting in caspase-3 activation. Conclusions : KRGE should be considered as a potential therapeutic drug for brain diseases including stroke induced by apoptosis of neuronal cells.

• 한국병원경영학회지
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• 제16권4호
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• pp.85-110
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• 2011

As Gangwon-do Province needs an environmental-friendly alternative tourism according to low carbon green growth, this study analyzes specialized fields in green health industry, selects seven specialized fields in green health industry that is fit to Gangwon-do Province, and suggests a development strategy by conducting recognition surveys on preference and impotence of the specialized fields. Surveys and interviews are conducted of 106 students at Green Health Industry Education Center which is operating by Kwandong University and 84 persons who work at medical institutions and tourism related companies in Ganwondo. The surveys and interviews were completed from November 5 to November 19 in 2010, determined factors through a factor analysis when they have many questions, and analyzed by using SPSS 14.0. Out of the seven specialized fields, rest & recuperation field is highly recognized. In order to have competitive edge compared to other self-governing provinces, Ganwon-do Province should prioritize rest and recuperation field. A healthcare program in the green health industry specific to Ganwon-do Province includes hot springs and spa programs as a priority. Gangwon-do's hot springs are deemed to be competitive resources as global medical tourists prefer spa and sauna healthcare programs, while the province promotes its spring resources and builds medical tourism infrastructures. Gangwon-do Province can promote a medical tourism industry that is well suitable for the strength and characteristics of the province. It might pursue oriental medicine therapy tourism, which is related to a recuperating medical service that uses both oriental and western medicine. It can also run such programs as forest bathing (oxygen road), spring and spa, and sea water treatment, which are the specialized fields in green health industry with respect to recreation and healthcare.

• 대한본초학회지
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• 제28권2호
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• pp.33-38
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• 2013

Objects : Apoptosis leads to the death of a cell. The mitochondrial pathway of apoptosis, a process regulated by the Bcl-2 family of proteins, plays a key role in various biological processes. The tuber of Liriope platyphylla Wang et TANG (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. The purpose of this study was to observe the effect of the Liriopis tuber on mitochondrial-mediated apoptosis in PC-12 cells. Method : A cytotoxic test on Liriopis Tuber water extract was conducted and another MTT assay was conducted to observe the cytoprotective effect against 4-HNE 25 ${\mu}M$ that causes oxidative stress in PC-12 cells for 24 hours. In addition, in order to observe the expression of Bcl-2 and Bax protein involved with apoptosis, western blot was conducted. Results : The LT water extract had no toxicity for PC-12 cell. In the cytoprotective effect against 4-HNE, both of the group treated with 50 ${\mu}g/m{\ell}$ and 100 ${\mu}g/m{\ell}$ of LT water extract showed a significant increase in comparison with the control group. In Bax protein expression, all the experimental groups treated with LT water extract showed a decrease in comparison with the control group but had no significance. In Bcl-2 protein expression, all the experimental groups treated with LT water extract showed a significant increase in comparison with the control group. Conclusion : These results suggest that LT is effective in reducing apoptosis.

• 간호행정학회지
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• 제8권4호
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• pp.585-594
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• 2002

Purpose : This study was to analyze on current Study situations were oriental-western hospital NMS of Korea ${\cdot}$ China. Method : This study was descriptive-comparative investigation. Study subjects were Korean(44) and Chinese(47) hospitals' NMS. Nursing division of Xi Yean Hospital of China Academy of Traditional Chinese Medicine collected raw data. Result : Hospital NMS of 44 Korean and 47 Chinese has been analyzed and results were as follows; Current situations of Korean NMS(47.7%) belonged to the Director of hospital while Chinese belonged to diagnosis and treatment division(78.7%), and to the nurse-vise superintendent(14.9%). Chinese NMS divided in nursing administration, and technology management that has unique type of simultaneous development in chinese medical and nursing practice. Korean(72%) and Chinese(43.3%) nursing division, and Chinese nursing unit(33.3%) operated inservice education. And Korea(43.2%) and China(80.9%) evaluated by written examination after education. Details of performed oriental nursing practices were similar between Korea and China. Conclusion : There were different of NMS, inservice education, but similar to oriental nursing practices between Korea and China.

• 동의신경정신과학회지
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• 제16권2호
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• pp.251-257
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• 2005

본 증례의 뇌경색 초피질감각실어증 환자는 한양방 치료 및 언어치료 시행을 통하여 K-WAB, K-BNT 등 표준화된 실어증 검사의 수행능력의 향상을 보였으며, 이야기 배열 과제에 대한 발화 분석에서 주제진술률의 향상, 의미착어와 음소착어의 감소 등 실제적 담화능력의 향상을 나타내었다. 실어증 환자의 경과관찰에서 이야기배열그림이 활용이 유용하리라 사료된다. 이후, 한방치료의 효과를 검증 및 이야기 배열과제 발화분석의 유용성에 대한 확대된 연구가 필요하다.

• 동의생리병리학회지
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• 제25권5호
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• pp.777-789
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• 2011

This study focuses on seeking a desirable direction for the discussions about identity issue of the current Traditional Korean Medicine(TKM). The identity issue of the current TKM is about asking what exactly the current TKM is, what sets the TKM apart from the other medical systems, and what value the TKM has in the contemporary settings, and seeking suitable answers to all these questions. The most important factor that affects these answers is what kind of the concept of identity we have. Current discussions of the identity issue of the TKM take narrow and inflexible concepts of identity such as "Numerical Identity," or "Qualitative Identity", not suitable for TKM, which is always changing and open to other academic fields. This study suggests that Hannah Arendt's concept of identity is more desirable for the TKM, which highlights pluralism and relations, as well as defining identity as a creative, positive, and open-ended process. In addition, this study also suggests that taking a closer look at the "field", which has previously been hidden behind the discourse on the "modernization", will help in figuring out what exactly the identity of the current TKM is. Lastly, the current discussions about "unification of Eastern and Western medical system", and "Integrative Medicine" in Korea will affect the identity issue of TKM.

• 대한한방소아과학회지
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• 제27권4호
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• pp.10-16
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• 2013

Objectives The purpose of this study was to investigate the acupuncture therapy about Attention Deficit Hyperactivity Disorder (ADHD) in China. Methods We searched for the studies on ADHD at China National Knowledge Infrastructure (CNKI, http://www.cnki.net) from 2011.1 to 2013.8. Results Selected 12 studies were divided into 7 case reports and 5 control studies. 7 case reports reported significant improvements with acupuncture therapy in ADHD. 5 control studies reported results that the acupuncture therapy groups were similar or better than western medicine treatment groups. Frequently used acupuncture points were EX-HN23 (7 times), GV20 ${\bullet}$ P6 ${\bullet}$ SP6 (6 times), G13 ${\bullet}$ EX-HN1 (5 times). Conclusions There have been reported many ADHD clinical studies in China and these studies can be applied to the clinical practices in Korea.