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Sequencing and Comparative Analysis of napA Genes from Helicobacter pylori Strains Associated with Iron-Deficiency Anemia

  • Hong, Myung-Hee;Choe, Yon-Ho;Cho, Yang-Je;Ahn, Bo-Young;Lee, Na-Gyong
    • Journal of Microbiology and Biotechnology
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    • v.15 no.4
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    • pp.866-872
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    • 2005
  • H. pylori is known to cause severe gastric diseases, including peptic ulcers and gastric cancers, and a link has also been suggested with iron-deficiency anemia (IDA). However, little is known about the pathogenesis of H. pylori-associated IDA. In the present study, to determine whether H. pylori strains are correlated with the prevalence of IDA, we analyzed and compared the sequences of the napA genes encoding a bacterioferritin-like protein in H. pylori strains. A total of 20 H. pylori strains were isolated from antral biopsies of patients with and without IDA, and the napA genes amplified from the genomic DNA were sequenced. A comparison of the deduced amino acid sequences for NapA revealed two sites with major variations. At residue 70, five out of the 12 non-IDA strains ($41.7\%$) contained serine, while only one of the 8 IDA strains ($12.5\%$) contained serine, indicating a significantly higher frequency of serine in the non-IDA strains. In addition, the NapA proteins from all 17 Western strains available on Web sites were found to contain serine residues at this position. Meanwhile, the other major variation was located at residue 73, where all eight IDA strains ($100\%$) contained leucine, while this was only true for eight of the 12 non-IDA strains ($66.7\%$). Therefore, these results indicated that the strains within each group were more genetically related to each other than to strains in the other group. When the expression level of the napA genes in the H. pylori strains was measured using RT-PCR, no significant difference was observed between the two groups, suggesting a similar intensity for the inflammatory responses induced by the NapA protein among the strains. Consequently, when taken together, the present data suggest that the occurrence of H. pylori-associated IDA may be partly determined by the infecting H. pylori strain, and the non-IDA strains are more closely related to Western strains than the IDA strains.

A Study on the Inter-constructive Design Dictionary through the Internet. (인터넷을 통한 상호구축적 디자인 용어사전의 연구)

  • 김태균
    • Archives of design research
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    • v.14 no.4
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    • pp.25-33
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    • 2001
  • With the increasing access to the internet, the number of designers who rely on internet to use information on design is on the rise. Therefore common dictionary of design terminology need to be formed and shared among designers. To do so, internet is very useful medium. However as relating terminology increases rapidly through interactivity among designers, it will be far from taking full advantage of features of internet to set up and provide such information unilaterally on internet. This indicates that providing data on the internet, not via traditional books, requires in-depth study on process of establishment of database structure and appropriate interface design. Thus this study will show design terms database model that harnesses internet feature that enables establishment of information spontaneously through user's interactivity, departing from a model that conveys information unilaterally. This report summarized and analyzed various models and suggested classification system in accordance with user's learning cognition. Problems on existing dictionary of design terminology were identified and new methods addressing such problems were exploited. In a word, this report is intended to propose user oriented inter-constructive database model that highlights high level of openness and interactivity by enabling changes of text in the cyber space and encouraging user to participate in making design dictionary.

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Analysis of Evaluation Indicators for the Development of Evaluation Models of Foreign Academic Journals (대학도서관의 외국학술지 평가모형 개발을 위한 평가지표 분석)

  • 김신영;이창수
    • Journal of the Korean Society for information Management
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    • v.21 no.2
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    • pp.45-67
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    • 2004
  • The purposes of this study are to compare and analyze the evaluation indicators for selection of journal suggested by scholars and organizations and to prepare theoretical background for the ideal model to meet opposing paradigms of collection management in academic libraries. A web survey method was employed to investigate applications of various selection criteria (27 for printed and 37 for electronic academic Journal) from the top 40 academic libraries in Korea. In addition, data were analysed statistically using factor analysis, t-test, Analysis of Variance(ANOVA), and Spearman's Rank Oder Correlation. The mean ranking for 9 evaluation indicators for printed were as follows: subscribing volumes per departments, degree of use, selection authority, electronic/print bundle, ISI impact factor, Internationality and reputation, costs for subscription, ILL & DDS, space considerations for printed materials. But, 11 evaluation indicators for electronic were as follows : costs for subscription, accessibility, electronic/print bundle, consortia, selection authority, access expandability, subscribing volumes per departments, scholarly features of the university, ISI impact factor, ILL & DDS, internationality and reputations.

A Case Study on Medical Library Collection Management Policies (의학도서관 장서관리정책 사례 연구)

  • Nam, Tae-Woo;Kim, Eun-Choo
    • Journal of the Korean Society for information Management
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    • v.27 no.1
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    • pp.79-102
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    • 2010
  • In Korea, more than half of medical libraries are small.sized hospital libraries operated under the single.librarian system, and it is keenly required to establish collection management policies for exchanging resources with other libraries and maintaining consistency. however, it is rare to set up their collection management policies on the web among the domestic medical libraries. Thus, this study purposed to survey cases of collection management policies in domestic and foreign medical libraries and to provide basic information and ideas for Korean medical libraries that are preparing collection management policies. As long as core requirements are satisfied, collection management policies do not have a way. Thus, this case study is expected to provide useful ideas to medical libraries developing their collection management policies. For this purpose, we selected 12 survey items based on previous studies, and investigated one domestic and five foreign medical libraries, and analyzed collected data. Based on the results of analyzing the cases, we suggested 12 guidelines.

A Usage Pattern Analysis of the Academic Database Using Social Network Analysis in K University Library (사회 네트워크 분석에 기반한 도서관 학술DB 이용 패턴 연구: K대학도서관 학술DB 이용 사례)

  • Choi, Il-Young;Lee, Yong-Sung;Kim, Jae-Kyeong
    • Journal of the Korean Society for information Management
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    • v.27 no.1
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    • pp.25-40
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    • 2010
  • The purpose of this study is to analyze the usage pattern between each academic database through social network analysis, and to support the academic database for users's needs. For this purpose, we have extracted log data to construct the academic database networks in the proxy server of K university library and have analyzed the usage pattern among each research area and among each social position. Our results indicate that the specialized academic database for the research area has more cohesion than the generalized academic database in the full-time professors' network and the doctoral students' network, and the density, degree centrality and degree centralization of the full-time professors' network and the doctoral students' network are higher than those of the other social position networks.

Performance Evaluation of Wireless and Wired Networks for Monitoring and Control of Indoor Air Quality(IAQ) in Subway Stations (지하철역사의 공기질 감시 및 제어를 위한 유무선 네트워크의 성능평가에 관한 연구)

  • Choi, Gi-Heung
    • Journal of the Korean Society of Safety
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    • v.27 no.1
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    • pp.1-6
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    • 2012
  • In crowded subway stations indoor air quality (IAQ) is a key factor for ensuring the safety and health of passengers. Since physical variables that describing IAQ such as the concentration of particulate, $CO_2$, VOCs and biological agents need to be closely monitored and controlled in multiple locations within subway stations and in remote sites, concept of web-based monitoring and control network using both wireless and wired media needs to be implemented. Connecting remote wireless sensor network and device (LonWorks) networks to the IP network based on the concept of VDN can provide a powerful, integrated, distributed monitoring and control performance. In this study, performance of wireless and wired network in VDN for monitoring and control of IAQ in subway stations is evaluated. Specifically, delay induced in wireless and wired networks, and data transmission rate are evaluated. A key parameter is identified in assuring safety and health of passengers in subway stations.

Responsiveness of Public Health Center and Its Related Factors against H1N1 Epidemic (신종플루 유행에 대한 보건소 담당자의 대응평가와 관련 요인)

  • Jang, Jung Lang;Kim, Keon Yeop;Hong, Nam Soo;Kam, Sin;Lee, Won Kee;Lee, Yu Mi
    • Health Policy and Management
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    • v.23 no.1
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    • pp.52-58
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    • 2013
  • This study was conducted to investigate the responsiveness and its related factors of public health center for novel influenza A (H1N1) epidemic. The data was collected through a web-based survey conducted during February to April 2011. The 182 respondents were team leaders or persons who were responsible for H1N1-related work at public health centers during the H1N1 prevalence. The related factors affecting the responsiveness were different by urban or rural area. In the level of gu (urban) area, cooperation with the public organizations, preparing its own response plan were the significant factors. But, in the level of si or gun (rural) area, cooperation with private organizations (clinic or pharmacy), physical (facilities, equipments, and medicines), and human infrastructures (public health professions, education and knowledge, and motivation) were more important factors. Therefore, how to cope with H1N1 prevalence in the future should be different by local characteristics. As a result, there are several challenges that public health centers should prepare for the further emerging infectious diseases. First, it is needed to make standard manuals which could strengthen education and training in order to respond appropriately, as well as to prepare enough physical infrastructures for the crisis. Next, the public health center should prepare correct media response and cooperation system with public and private organizations.

Identification of Candidate SNP (Single Nucleotide Polymorphism) for Growth and Carcass Traits Related to QTL on Chromosome 6 in Hanwoo (Korean Cattle)

  • Lee, Y.S.;Lee, J.H.;Lee, J.Y.;Kim, J.J.;Park, H.S.;Yeo, J.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.12
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    • pp.1703-1709
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    • 2008
  • As genetic markers, single nucleotide polymorphisms (SNP) are very appropriate for the development of genetic tests for economic traits in livestock. Several microsatellite markers have been identified as useful markers for the genetic improvement of Hanwoo. Among those markers, ILSTS035 was recently mapped at a similar position with four SNPs (AH1_11, AH1_9, 31465_446, and 12273_165) in a linkage map of EST-based SNP in BAT6. Among the four SNPs, two SNPs (31465_446 and 12273_165) were analyzed using BLAST at the NCBI web site. The sequences including the 12273_165 SNP were identified at the intron region within the LOC534614 gene on the gene sequence map (Bos taurus NCBI Map view, build 3.1). The LOC534614 gene represents a protein similar to myosin heavy chain, fat skeletal muscle, embryonic isoform 1 in the dog, and myosin_1 (Myosin heavy chain D) in Macaca mulatta. In cattle, the myosin heavy chain was associated with muscle development. The phenotypic data for growth and carcass traits in the 415 animals were analyzed by the mixed ANCOVA (analysis of covariance) linear model using PROC GLM module in SAS v9.1. By the genotyping of Hanwoo individuals (n = 415) to evaluate the association of SNP with growth and carcass traits, it was shown that the 12273_165 SNP region within LOC534614 may be a candidate marker for growth. The results of the statistical analyses suggested that the genotype of the 12273_165 SNP significantly affected birth weight, weight of the cattle at 24 months of age, average daily gain and carcass cold weight (p<0.05). Consequently, the 12273_165 SNP polymorphisms at the LOC534614 gene may be associated with growth in Hanwoo, and functional validation of polymorphisms in LOC534614 should be performed in the future.

A Study on the online of PDF Electronic Documents System (인터넷 원거리출판의 응용과 PDF의 인쇄활용에 관한 연구)

  • 유영수;강영립;김병현;이광수
    • Proceedings of the Korean Printing Society Conference
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    • 2001.06a
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    • pp.63-77
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    • 2001
  • PDF(Portable Document Format) is a file format that Adobe advances postscritp technique and use in managing document information or electric publishing(internet, CD-ROM, DVD). PDF is a devised document type for being able to read and print anywhere, independent of OS, printer type, resolution, and the kind of computer etc. Because this includes a compressing function, it transfers document through a small size of file in internet or intranet. In addition, that is a file format has various advantages-sharing of information and transfering documents in on line or off line environment. In this paper, we developed electronic document system using PDF format. Electronic document system consists of filter, automatic indexing, special searching system and web server. The information used in this paper is database made using Zwon\`s DocuCom. The filter recognizes various kinds of document structure. And according to property of document, it produces ASCII output. In addition to processing various formats of document, the filter can extract keywords in documents of MS WORD, Excel, Powerpoint, PDF, CAD etc. This filter uses the structure of window printer drive and can extract the information for text, page, font type and size from relevant document. The automatic indexing recognizes the formatted tag of document form ASCII text produced by filter and extracts adequate keyword to structure and property of document. PDF electronic document systems proposed in this paper can be used in Internet, PC communication. Users can choose and read electronic documents by two ways. First, users can choose and read relevant books using PDF electronic document homepage. Second, users can use PDF integrated-search system. User can search after inputing keyword and choose reference field and type of data. But, now, PDF products of Adobe can\`t support the Korean character. If this problem is resolved, we thick that PDF applications system looks active. Although there is limited function in case of using Zwon DocuCom used in this study, we think that there isn\`t a great deal of difficulty in electronic document and building digital database.

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An Analysis of the Vulnerability of SSL/TLS for Secure Web Services (안전한 웹 서비스를 위한 SSL/TLS 프로토콜 취약성 분석)

  • 조한진;이재광
    • Journal of the Korea Computer Industry Society
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    • v.2 no.10
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    • pp.1269-1284
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    • 2001
  • The Secure Sockets Layer is a protocol for encryption TCP/IP traffic that provides confidentiality, authentication and data integrity. Also the SSL is intended to provide the widely applicable connection-oriented mechanism which is applicable for various application-layer, for Internet client/server communication security. SSL, designed by Netscape is supported by all clients' browsers and server supporting security services. Now the version of SSL is 3.0. The first official TLS vl.0 specification was released by IETF Transport Layer Security working group in January 1999. As the version of SSL has had upgraded, a lot of vulnerabilities were revealed. SSL and TLS generate the private key with parameters exchange method in handshake protocol, a lot of attacks may be caused on this exchange mechanism, also the same thing may be come about in record protocol. In this paper, we analyze SSL protocol, compare the difference between TLS and SSL protocol, and suggest what developers should pay attention to implementation.

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