• Food Science and Biotechnology
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• 제15권1호
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• pp.138-142
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• 2006

Various levels of antibacterial activity have been identified for water and ethanol extracts of corn silk, particularly against Salmonella typhimurium KCTC 2515. In general, the water extract was more effective than the ethanol extract. The minimum inhibitory concentration (MIC) for the water extract was 7.5 mg/disc for S. typhimurium KCTC 2515 and B. cereus KCTC 1092, as well as for the ethanol extract against S. typhimurium KCTC 2515 and S. typhimurium KCTC 1925. However, the MICs for the water extract were lower than those for the ethanol extract against all bacteria tested, except S. typhimurium KCTC 1925 and B. cereus KCTC 1014. The growth of the tested organisms in the synthesized broth medium was inhibited with the addition of 5-fold levels of MIC. Using sterilized milk as the model food system, we found that the lag phase for these microorganisms was extended up to 3 days at $20^{\circ}C$, but was not affected at $4^{\circ}C$. These results indicate that bacterial growth was strongly inhibited by corn silk extract at $20^{\circ}C$.

• 한국식품조리과학회지
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• 제16권5호
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• pp.390-393
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• 2000

Salmonella typhimurium TA98 streptomycin 의존성 SD510 균주를 사용하여 녹차의 수용성 추출물과 에탄을 용해성 추출물에 대한 항변이 효과를 검토하였다. 4-NQO에 대한 항변이 활성은 수용성 추출물과 에탄을 용해성 추출물 모두에서 추출물의 투여량이 증가할수록 높았으며, 억제효과는 5월과 8월 순 녹차 추출물을 1,000$\mu\textrm{g}$/plate 투여했을 때 각각 93% 및 95%로 나타났다. Trp-P-1에 대한 항변이 활성은 에탄을 용해성 추출물의 경우 53.3~921.%로서 투여농도가 증가할수록 억제 효과가 높았다. 그러나 수용성 추출물은 투여농도가 증가할수록 항변이 활성이 감소하였다.

• 대한본초학회지
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• 제23권3호
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• pp.41-51
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• 2008

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of extracts from Scutellaria baicalensis, Zizyphus jujuba and Atractylodes macrocephala. Methods : Three kinds of medicinal herbs were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidant activity and growth inhibition activity against cancer cells. Results : Electron donating abilities (EDA) of the water and ethanol extract from Zizyphus jujuba and Atractylodes macrocephala were over than 87% at 1,000 ${\mu}g/mL$, respectively. The highest nitrite scavenging abilities (NSA) of the water and ethanol extracts from Scutellaria baicalensis were 68.9 and 79.2% at 1,000 ${\mu}g/mL$, respectively. Atractylodes macrocephala extracts had shown the highest growth inhibition activity against both of MDA and A549 cells and the water extract had higher activity than ethanol extract. Conclusions : These results suggest that the extracts of medicinal herbs used for this experiment had specific biological activity and they can be used as natural antioxidant to prevent oxidative damage in normal cells.

• 약학회지
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• 제50권6호
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• pp.421-428
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• 2006

Water extracts, ethanol extracts, and juice of Cusuta japonica, C, australis, and C. chinensis were prepared, and their cytotoxicity, antioxidant activity and inhibitory effects on tyrosinase activitiy and melanin biosynthesis were estimated by using melanoma Clone M-3. From this study; the following conclusions were attained. Extracts of Cuscuta japonica, C. australis, and C. chinensis showed noticeable cytotoxicity except ethanol extracts from the stem of C. australis. A maximual cytotoxicity was observed with tile ethanol extract from the seed of C, australis (87.39%). While the ethanol extract from the seed of C. japonica (91.88%) showed the most pronounced inhibitory effect on melanin biosynthesis, the water extract from the stem of C. japonica (1.05%) possessed very little inhibitory effect. The most inhibitory effect on tyrosinase activity was observed with the water extract from the stem of C. australis (76.67%). Howeverr the water extract from the stem of C. japonica showed a very poor effect on the inhibition of tyrosinase activity All the preparations, except extracts from the seed of C. australis were able to remove reactive oxygen species (ROS) in a dose-dependent manner. The juice of C. japonica demonstrated the strongest activity (59.02%).

• 한국양봉학회지
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• 제32권4호
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• pp.391-394
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• 2017

Propolis is made by bees collecting protective material or essence of plants and mixing with saliva and enzymes produced by the salivary glands. It is used to repair the inside of the honeycomb, keep it sterile, and adjust the temperature and humidity. Propolis is a natural antibiotic substance that it is used to make a clean room by coating the cell before the queen bee lay eggs, and preventing the bacteria from invading by using with wax when sealing the nursery room. Propolis extract is a health functional food with antioxidant and oral antimicrobial effects. In order to use propolis in food, its active ingredients are extracted with ethanol. Water-soluble propolis was prepared by mixing and stirring honey and ethanol extracted propolis (EEP) solution. When 1kg of honey and 100ml of ethanol extracted propolis solution were mixed and stirred, the total flavonoid content of water-soluble propolis was $6.6{\pm}1.1mg/10g$, and the free radical scavenging effects of water-soluble propolis were 54 to 74%.

• 한국식품저장유통학회지
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• 제23권1호
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• pp.20-26
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• 2016

본 연구에서 92종의 한약재로부터 물과 ethanol을 용매추출물에 대하여 HAase의 저해에 의한 항염증 효과를 측정한 결과 물 추출물에서는 E. officinalis(86.8%), T. orientalis(80.8%), C. semen(66.5%), M. azedarach(74.7%), S. pubescen (61.3%), S. chinensis(49.15%) 등이 높게 나타났다. Ethanol 추출물에서는 A. altissima와 S. chinensis 추출물이 90% 이상의 높은 항염증 활성을 나타내었다. 이들 중 물과 에탄올 추출물에서 모두 항염증 활성이 높게 측정된 S. chinensis가 선발되었다. 선별된 삼백초의 유효성분을 보다 효율적으로 추출할 방안을 확립하기 위하여 인체에 유해하지 않은 용매로 물과 ethanol을 선택하여 추출 최적조건을 살펴보았다. Phenolic 성분의 추출을 위한 최적조건은 50% ethanol을 사용하여 12시간 추출이 최적이었다. Phenolic 성분의 추출을 위해서는 ethanol이 물보다 더 효율적이었다. 최적조건에서 추출된 삼백초 추출물의 염증억제 활성은 $100{\sim}250{\mu}g/mL$ phenolic 농도로 첨가했을 때 70~80%의 염증 억제 효과를 나타내었으나, $500{\mu}g/mL$ 이상의 농도로 첨가 시 오히려 염증 억제활성이 감소하는 경향을 나타내었다. 삼백초를 초미세 분쇄 하였을 때 추출 수율과 염증억제 활성이 증가하는 것을 확인하였다. 따라서 초미세 분쇄 기술은 한약재로부터 생리활성물질 추출수율의 증가를 목적으로 적용할 수 있는 기술로 개발이 가능할 것으로 판단되었다.

• 생명과학회지
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• 제25권4호
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• pp.425-432
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• 2015

각각의 나라는 민족의 공감성과 문화성이 깃든 고유의 술을 가지고 있으며, 우리나라에는 고유의 전통주 막걸리(탁주)가 있다. 본 연구에서는 주박을 이용한 고부가가치 향장소재 개발을 목표로, 상업적 시설에서 대량생산된 우국생 주박의 ethanol 및 열수 추출물로부터 n-hexane, ethylacetate, butanol을 이용한 순차적 유기용매 분획물과 물 잔류물을 조제한 후, 이들의 혈액응고 저해활성, 혈소판 응집저해 활성 및 인간 적혈구 용혈활성 및 항산화 활성을 평가하였다. 우국생 주박의 수분함량은 80.3%, pH 3.94, brix 13.0 및 알코올 함량은 약 1.8%이었으며, ethanol 추출의 경우 추출효율은 6.62%로 열수 추출의 2.1%보다 3.15배 높은 수율을 나타내었다. 총폴리페놀 및 총플라보노이드 함량 분석결과 주박 ethanol 추출물의 ethylacetate 분획물에서 가장 높은 함량을 나타내었으며, 특히 폴리페놀 함량은 매우 높게 나타났다(128 mg/g). 혈액응고 저해활성을 평가한 결과, ethanol 추출물의 ethylacetate 분획물에서는 5 mg/ml 농도에서 TT, PT, aPTT 모두 15배 이상의 연장된 응고시간을 나타내어 매우 강력한 thrombin, prothrombin 및 coagulation factor 저해활성을 나타내었다. 혈소판 응집저해능 평가에서는 ethanol 추출물의 butanol 분획 및 물 잔류물에서 항혈전제로 사용되고 있는 aspirin 보다 우수한 응집저해능을 확인하였다. 상기 10종 시료들은 0.5 mg/ml 농도까지 적혈구 용혈활성이 나타나지 않았으며, 양호한 radical 소거능을 나타내었다. 본 연구결과는 사료 및 비료로 활용되고 있는 우국생 주박의 활성분획물을 이용하는 경우 고부가가치 항혈전제 개발이 가능함을 제시하고 있다.

• Environmental Analysis Health and Toxicology
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• 제3권1_2호
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• pp.55-64
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• 1988

Experiments were performed on mice to investigate the influences of ampicillin and ethanol on the immune response. Ampicillin was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. 1. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the weight of spleen, but increased the weight of thymus. 2. Humoral immune response was slightly reduced by ampicillin. Especially, the combined administration of ampicillin and ethanol significantly reduced hemclysin production. 3. Cellural immune response was reduced by ampicillin. The combine administration of ampicillin and ethanol significantly reduced cellural immune response. 4. Peripheral circulating white blood cell was reduced by the combined administration of ampicillin and ethanol as compared to ampicillin. 5. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the phagocyte activity.

• Environmental Analysis Health and Toxicology
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• 제4권1_2호
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• pp.1-10
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• 1989

Experiments were performed on mice to investigate the influences of doxycycine and ethanol on the immune responses. Doxycycline was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. Pathotoxicological influences were measured by serum protein and albumin. The weight of spleen, thymus and liver were measured. Doxycline and ethanol combined administration decreased the weight of thymus and spleen. Humoral and cellular immune response were reduced by doxycycline administration. Especially ethanol combined administration significantly reduced humoral and cellular immune response. Phagocyte activity was increased by ethanol combined administration and peripheral circulating white blood cell was significantly increased by ethanol adminstration. Ethanol combined administration decreased serum A/G ratio.

• 청정기술
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• 제24권3호
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• pp.239-248
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• 2018

본 연구에서는 (주)파인텍에서 개발한 제올라이트 4A 분리막을 이용하여 물, 에탄올, 이소프로필알코올 단일 성분 및 혼합물의 투과증발 실험을 수행하였다. 본 분리막은 수열합성법을 이용하여 제막하였고, Si/Al 비율이 1인 LTA 구조에 $Na^+$를 이온교환하여 약 $4{\AA}$의 기공크기를 갖고 있으며, 강한 친수성을 나타내고 있다. 물리적 특성을 확인하기 위해 SEM, porosimetry, BET, 압축강도계를 이용하였다. 다양한 온도 및 농도 조건 실험을 통해 제올라이트 4A 분리막이 물/에탄올(분리계수 3,000 이상) 및 물/이소프로필알코올(분리계수 1,500 이상) 혼합물로 부터 물을 선택적으로 분리할 수 있음을 확인하였다. 활동도계수, Generalized Maxwell Stefan 모형 및 Dusty Gas 모형을 이용하여 단일성분 및 혼합물의 투과증발 거동을 모사하였으며, Genetic Algorithm를 이용한 상수추정을 통하여 분리층의 흡착 및 확산상수를 구하였다.