• 제목/요약/키워드: Water temperature stress

검색결과 647건 처리시간 0.029초

Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

  • Pulla, Rama Krishna;Kim, Yu-Jin;Kim, Myung-Kyum;Senthil, Kalai Selvi;In, Jun-Gyo;Yang, Deok-Chun
    • BMB Reports
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    • 제41권4호
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    • pp.338-343
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    • 2008
  • Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.

Solid-Phase Speciation of Copper in Mine Wastes

  • Jeong, Jae-Bong
    • Bulletin of the Korean Chemical Society
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    • 제24권2호
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    • pp.209-218
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    • 2003
  • Ecosystems in the Keweenaw Peninsula region of Lake Superior, USA, were disturbed by over 500 million tons of copper-rich mine tailings during the period 1850-1968. Metals leaching from these mine residues have had dramatic effects on the ecosystems. Vast acreages of exposed tailings that are over 100 years old remain unvegetated because of the combination of metal toxicity, absence of nutrients, and temperature and water stress. Therefore, it is important to characterize and fractionate solid copper phases for assessing labile forms of copper in soils and sediments contaminated by the mining wastes. X-ray diffraction analyses indicate that calcite, quartz, hematite, orthoclase, and sanidine minerals are present as major minerals, whereas cuprite,tenorite, malachite, and chalcopyrite might be present as copper minerals in the mining wastes. Sequential extraction technique revealed that carbonate and oxide fractions were the largest pools of copper (ca. 50-80%) in lakeshore and wetland stamp sands whereas the organic matter fraction was the largest reservoir (ca. 32%) in the lake sediments. The concentrations of iron and copper were inversely correlated in the oxide fraction suggesting that copper may occur as a surface coating on iron oxides. As particle size and water contents decrease, the percent of the copper bound to the labile carbonate fraction increases.

Luteolin and Chicoric Acid, Two Major Constituents of Dandelion Leaf, Inhibit Nitric Oxide and Lipid Peroxide Formation in Lipopolysaccharide-Stimulated RAW 264.7 Cells

  • Park, Chung-Mu;Park, Ji-Young;Song, Young-Sun
    • Preventive Nutrition and Food Science
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    • 제15권2호
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    • pp.92-97
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    • 2010
  • Luteolin and chicoric acid are the most abundant phytochemicals in dandelion (Taraxacum officinale) leaf. In this study, four kinds of extraction methods [hot water, ambient temperature (AT) water, ethanol, and methanol] were applied to analyze the contents of both phytochemicals and verify their anti-inflammatory and antioxidative activities. The methanol extract showed the most potent nitric oxide (NO) inhibitory effect. The luteolin and chicoric acid concentrations were 3.42 and $12.86\;{\mu}g/g$ dandelion leaf in the methanol extract. The NO-suppressive effect of luteolin and chicoric acid was identified in a dose-dependent manner with $IC_{50}$ values of $21.2\;{\mu}M$ and $283.6\;{\mu}M$, respectively, in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without cytotoxicity. Malondialdehyde (MDA) concentration, as an index for free radical injury on cell membrane, was also dose-dependently inhibited by the two compounds. The suppressive effect was further examined using mRNA and protein expression levels, which were attributable to the inhibition of inducible nitric oxide synthase (iNOS). These results suggest that two phytochemicals in dandelion leaf, luteolin and chicoric acid, may play an important role in the amelioration of LPS-induced oxidative stress and inflammation.

고성능 콘크리트의 폭렬특성 및 대책 (Countermeasure and Spalling Property of High Performance Concrete)

  • 한민철;한천구
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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    • pp.1105-1108
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    • 2008
  • 본 고에서는 화재 시 구조물 콘크리트의 안전성 확보를 목적으로, 폭렬방지대책 및 메커니즘에 대해 고찰 분석한 것으로서, 그 결과를 요약하면 다음과 같다. 1) 국내 외에 알려져 있는 고성능 콘크리트의 폭렬방지 방법으로 함수율 및 C/W를 낮추는 방법,내화피복을 실시하여 고온을 차단하는 방법, 횡구속 실시하여 내부에서 발생되는 횡변위에 저항하는 방법, 섬유를 혼입하여 수증기압을 외부로 배출시키는 방법 등 4가지 방안이 있다. 2) 기존의 폭렬관련 이론과 WPB이론에 의한 폭렬메커니즘 분석을 토대로 폭렬방지 방안을 제안하면 기존 건축물에 한해서는 내화피복을 실시하는 방법이, 신축 건축물에 한해서는 섬유를 혼입하는 방법이 양호한 것으로 판단된다.

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Environmental Factors and Bioremediation of Xenobiotics Using White Rot Fungi

  • Magan, Naresh;Fragoeiro, Silvia;Bastos, Catarina
    • Mycobiology
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    • 제38권4호
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    • pp.238-248
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    • 2010
  • This review provides background information on the importance of bioremediation approaches. It describes the roles of fungi, specifically white rot fungi, and their extracellular enzymes, laccases, ligninases, and peroxidises, in the degradation of xenobiotic compounds such as single and mixtures of pesticides. We discuss the importance of abiotic factors such as water potential, temperature, and pH stress when considering an environmental screening approach, and examples are provided of the differential effect of white rot fungi on the degradation of single and mixtures of pesticides using fungi such as Trametes versicolor and Phanerochaete chrysosporium. We also explore the formulation and delivery of fungal bioremedial inoculants to terrestrial ecosystems as well as the use of spent mushroom compost as an approach. Future areas for research and potential exploitation of new techniques are also considered.

구스 아스팔트의 열 영향이 3경간 연속 강상판 박스 거더교의 반력에 미치는 영향 (The Variety of Reaction at the Three Span Continuous Steel Box Girder Bridge under The Thermal Effect of Guss Asphalt)

  • 김성남;고윤기;한택희;강영종
    • 한국전산구조공학회:학술대회논문집
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    • 한국전산구조공학회 2004년도 가을 학술발표회 논문집
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    • pp.195-202
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    • 2004
  • After the 1990's, Guss asphalt has chosen in Korea for using in pavement of a steel deck bridge because of it's distinguish ability about water proof and so on. But on the other hand it may cause server stress and displacement on the bridge as it is treated using very high temperature ranging from 220℃ to 260℃. Therefore it is critical to estimate the thermal effect of Guss asphalt on the steel deck bridge before the width and pattern of the unit portion are decided to minimize impact. In this study, a serious of numerical tests of the some steel box bridges were idealized were conducted to verify the feasibility of numerical value analysis. The parametric study was performed to present design proposal about the Box section dimensions and the diaphragm spacing.

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가스 하이드레이트가 매장된 해저사면의 붕괴에 관한 기초적 연구 (A Preliminary Study on Submarine Slope Failure of Gas Hydrate-bering Sediments)

  • 박성식
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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    • pp.399-404
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    • 2008
  • The influence of gas hydrate dissociation on submarine slope stability was studied in this paper. Gas hydrates are stable under high pressure and low temperature conditions. Once gas hydrate dissociates due to natural or human activities, it generates large amount of gas and water. During gas hydrate dissociation, a pore pressure between soil particles increases and results in the loss of an effective stress and degradation of soil stiffness. A pore pressures model was proposed to calculated excess pore pressures generated by gas hydrate dissociation at the Storegga Slide. A slope stability analysis for the Storegga Slide using a two dimensional finite difference method was carried out by considering excess pore pressures due to gas hydrate dissociation. Since the excess pore pressure calculated by the proposed method resulted in the considerable loss of stiffness and strength in slope, a submarine slope failure occurred at the Storegga slide was well simulated.

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가속열화 방법에 의한 주상변압기 절연물의 열 열화 특성 평가 (The Evaluation of Thermal Aging Characteristics in Insulating Materials of the Pole Transformers)

  • 이병성;송일근;이재봉;한상옥
    • 한국전기전자재료학회논문지
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    • 제16권12호
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    • pp.1136-1141
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    • 2003
  • The primary insulation materials used in an oil filled transformer are kraft paper, wood, porcelain and oil. Modern transformers use chemically treated paper to improve its tensile strength and resistance to aging caused by immersion in oil. But these insulation papers are mainly aged by thermal stress. Over the life time of the insulation paper and oil, it is exposed to high temperatures, oxygen and water. Its interaction with the steel of the tank and core plus the copper and aluminium of the windings will eventually cause the chemical properties of the oil to decay. High temperature have an effect on mechanical strength of cellulous paper used in the layer insulation. We made two aging cells in which insulation papers and mineral oil are conducted to test thermal properties. It is measured dielectric strength, number of acid, moisture, etc. of insulation paper and oil aged in the aging cells.

변압기 층간 절연지의 열열화 특성 평가에 관한 연구 (The Study on Characteristics for Thermal Aging of the Layer Insulation in Transformers)

  • 이병성;송일근;김동명;박동배;한상옥
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2002년도 하계학술대회 논문집
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    • pp.406-409
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    • 2002
  • The primary insulation system used in an oil-filled transformer is Kraft paper, wood, porcelain and, of course, oil. Modern transformers use paper that is chemically treated to improve its tensile strength properties and resistance to aging caused by immersion in oil. These insulation papers are mainly aged to thermal stress. Over the course of the insulation paper and oil's life it is exposed to high temperatures, oxygen and water. Its interaction with the steel of the tank and core plus the copper and aluminium of the windings will eventually cause the chemical properties of the oil to decay. High temperature have an effect on mechanical strength of cellulous paper using the layer insulation. We made two aging cell in which thermal aging tests of insulation papers and mineral oil are conducted. It is measured dielectric strength, number of acid, moisture, etc. of insulation paper and oil aged in the aging cells.

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Zooplankton community distribution in shallow reservoirs during winter: Influence of environmental factors on Cyclops vicinus (Copepoda: Cyclopoida)

  • Choi, Jong-Yun;Jeong, Kwang-Seuk;Joo, Gea-Jae
    • Journal of Ecology and Environment
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    • 제37권2호
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    • pp.99-104
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    • 2014
  • We estimated the influence of environmental factors on zooplankton communities at 25 reservoirs during winter (December 2010 to January 2011). Among zooplankton groups, Cyclops vicinus is more dominant during winter, and this is positively related to withered vegetation area and dissolved oxygen level. Therefore, the presence of withered vegetation might be considered as an important factor to determine C. vicinus distribution during winter. We considered that withered vegetation might be utilized as a habitat for C. vicinus, as well as provide an attachment substrate for periphytic algae. Abundance of periphytic algae can lead to high concentration of dissolved oxygen. Although copepods prefer high water temperatures for increasing their population growth, if Cyclops can overcome low temperature stress that leads to disruption of population, their population growth initiation in the next growing season (i.e. next spring) is possibly propelled by the winter population.