• 환경생물
• /
• 제33권4호
• /
• pp.450-458
• /
• 2015

The heat shock proteins (Hsps), one of the most highly conserved groups of proteins, play crucial roles in protecting cells against environmental stressors, such as temperature, salinity, heavy metals and pathogenic bacteria. The glutathione S-transferases (GST) have important role in detoxification of oxidative damage, environmental chemicals and environmental stress. The purpose of this study is to investigate the gene expression of Hsp70 and GST on change of temperature and salinity in Mytilus coruscus. The M. coruscus was cultured in incubator of separate temperature and salinity (8, 20, $30^{\circ}C{\times}20$‰, 25‰, 30‰) for 28 days. Ten individuals in each group were selected after each 14 and 28 days exposure. Results that the expression of Hsp70 mRNA was no significant changed in M. coruscus exposed to temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$) and salinity (20‰, 25‰, 30‰) for 14 days. Whereas the expression of Hsp70 mRNA was increased in exposure to temperature $30^{\circ}C$ and salinity (20‰, 25‰, 30‰) for 28 days. The expression of GST mRNA was increased in exposure to temperature $30^{\circ}C$, salinity (25‰, 30‰) for 14 days and temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$), salinity (20‰, 25‰, 30‰) for 28 days. These results suggest that Hsp70 and GST were played roles in biomarker gene on the thermal and salinity stress.

• 한국어류학회지
• /
• 제28권1호
• /
• pp.1-8
• /
• 2016

본 연구는 능성어 (Epinephelus septemfasciatus)의 겨울철 저수온으로 발생하는 폐사원인 규명을 위한 생리생화학적인 반응을 조사하기 위하여 수온 $12^{\circ}C$를 대조구로 Exp. I ($-1^{\circ}C/24h$), Exp. II ($-1^{\circ}C/8h$)의 실험구를 정하여 9일간에 걸쳐 생존율, 코티졸, 글루코스, 총단백질 및 전해질 농도를 측정하였다. 생존율은 Exp. I 실험구의 경우 사육수온 $5^{\circ}C$로 하강한 후 170시간째 12마리의 폐사를 시작으로 174시간째에 전 개체 폐사하였다. 반면 Exp. II 실험구의 경우 $5^{\circ}C$로 하강한 후 72시간째 9 마리의 폐사를 시작으로 76시간째 전 개체가 폐사하였다. 생리적 반응은 Exp. I과 Exp. II의 미비한 차이는 있으나 비슷한 경향이 나타났으며, 혈장 코티졸과 글루코스의 대사생리가 $7^{\circ}C$에 급격하고 상승하고, 유영능력과 아가미 개폐운동이 빨라졌다. 이상의 결과로 능성어의 저수온 내성의 임계수온은 $7^{\circ}C$로 추정된다.

• 암석학회지
• /
• 제21권2호
• /
• pp.249-261
• /
• 2012

감람석은 맨틀 상부의 주요 광물이고, 탄성적으로 매우 비균질하다. 맨틀암석이 고압고온에서 변형될 때, 이러한 감람석이 배열되어 격자선호방향(LPO, Lattice Preferred Orientation)이 형성된다. 감람석의 격자선호방향은 압력, 응력, 그리고 감람석 내부의 물(OH)의 양에 따라 크게 영향을 받을 수 있다. 이 논문에서는 물, 응력, 그리고 압력이 어떻게 감람석의 격자선호방향(LPO)의 형성에 영향을 주는지에 대해 그동안 발표된 논문들을 리뷰했고, 자연 맨틀암석에서 발견된 감람석의 격자선호방향들을 요약했으며, 감람석의 격자선호방향과 맨틀상부에서 나타나는 지진파의 비등방성과의 연계성에 대해 토의하였다. 그리고 최근에 맨틀암석에서 발견된 사방휘석의 네 가지 격자선호방향들을 기술하였다.

• 한국농업기계학회:학술대회논문집
• /
• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.III
• /
• pp.593-599
• /
• 2000

Accurate quantification of plant physiological properties is often necessary for optimal control of an automated greenhouse production system. Conventional crop growth monitoring systems are usually burdensome, inaccurate, and harmful to crops. A thermal image analysis system was used to accomplish rapid and accurate measurements of physiological-property changes of water-stressed crops. Thermal images were obtained from several species of plants that were placed in a growth chamber. Analyzing the images provided the pattern of temperature changes in a leaf and the amount of differences in the temperature of stressed plants and non-stressed plants.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 2002년도 봄 학술발표회 논문집
• /
• pp.41-46
• /
• 2002

Recently, the design and construction of massive concrete structures are increased, But, the temperature rise within a large concrete mass makes the construction of massive concrete structures be very difficult. Therefore, various techniques of the thermal stress control of the mass concrete have been widely used. One of them is pipe-cooling which reduces the temperature of concrete with flowing water. It was shown to be possible to construct the massive concrete foundation of urban bridge successfully by application of pipe-cooling system with steel pipe and water circulation. It was also found to expected to make it possible to reduce the probability of thermal crack development in a massive concrete foundation of urban bridge by pipe-cooling system.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2000년도 추계학술대회논문집B
• /
• pp.228-233
• /
• 2000

The Korean Next Generation Reactor(KNGR) is a Pressurized Water Reactor adopting direct vessel injection(DVI) to optimize the performance of emergency core cooling system(ECCS). In a certain accident, however, pressurized thermal shock(PTS) of the vessel due to the sudden contact with the injected cold water is expected. In this paper, an accident of Main Steam Line Break(MSLB) has been numerically investigated with direct vessel injections and an increased volume flow rate in some cold legs. Using FLUENT code, temperature distributions of the fluid in the downcomer and of reactor vessel including the core region have been calculated, together with the distribution of convective heat transfer coefficient(CHTC) at the cladding surface of the reactor vessel. The result shows that some parts of the core region of the reactor vessel have higher temperature gradient expressing higher thermal stress.

• 한국재료학회지
• /
• 제13권4호
• /
• pp.224-227
• /
• 2003

The sliding wear behavior of a Fe-base hardfacing alloy was investigated in the temperature range of $25∼250^{\circ}C$ under a contact stress of 15 ksi (103 MPa). The wear loss of this Alloy in pressurized water was less than that of NOREM 02. And galling did not occurred at this alloy in all temperature ranges. It was considered that the wear resistance of this Alloy was attributed to the strain-induced phase transformation from austenite to $\alpha$'martensite during sliding wear.

• 한국해양공학회지
• /
• 제18권4호
• /
• pp.46-51
• /
• 2004

The surface layer in dredged and reclaimed marine clay is improved by mixing of shallow soils and hardening agents, which is made of cement, containing some other special admixtures. Tests in both laboratory and field settings are performed to investigate the improvement effect and strength properties of cement-stabilized soils. The test results show that the hardening agent sufficiently improves the soil properties of the surface layer, while increasing the load-carrying capacity. The strength of cement-stabilized soils depends, primarily, on water-to-cement ratio and curing temperature. That is, the higher curing temperature and the longer curing time, the higher the strength in cement-stabilized soils. The high ratio of water-ta-cement results in a lower strength.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권3호
• /
• pp.435-441
• /
• 2015

Relationships among different stress parameters (lairage time and blood level of lactate and cortisol), meat quality parameters (initial and ultimate pH value, temperature, drip loss, sensory and instrumental colour, marbling) and carcass quality parameters (degree of rigor mortis and skin damages, hot carcass weight, carcass fat thickness, meatiness) were determined in pigs (n = 100) using Pearson correlations. After longer lairage, blood lactate (p<0.05) and degree of injuries (p<0.001) increased, meat became darker (p<0.001), while drip loss decreased (p<0.05). Higher lactate was associated with lower initial pH value (p<0.01), higher temperature (p<0.001) and skin blemishes score (p<0.05) and more developed rigor mortis (p<0.05), suggesting that lactate could be a predictor of both meat quality and the level of preslaughter stress. Cortisol affected carcass quality, so higher levels of cortisol were associated with increased hot carcass weight, carcass fat thickness on the back and at the sacrum and marbling, but also with decreased meatiness. The most important meat quality parameters (pH and temperature after 60 minutes) deteriorated when blood lactate concentration was above 12 mmol/L.

• Journal of Plant Biotechnology
• /
• 제44권3호
• /
• pp.287-295
• /
• 2017

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.