• Journal of Life Science
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• v.24 no.11
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• pp.1209-1216
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• 2014

The aim of this study was to investigate the effect of water-soluble sulfated ${\beta}$-glucan (SBG) obtained from Ganoderma lucidum mycelia on the antihyperlipidemic and serum lipid levels in high-fat diet-induced obese rats. Five-week-old male Sprague-Dawley rats were fed a high-fat diet for two weeks to induce obesity. They were ten divided into five groups-normal control diet group (NC), high-fat control diet group (HC), high-fat diet and 200 mg/kg of SBG group (HC-HSBG), high-fat diet and 20 mg/kg of SBG group (HC-LSBG), and high-fat diet and 20 mg/kg of lovastatin group (HC-Lov)-and fed one of five diets for two more weeks. Although food intake and final body weight after four weeks of SBG consumption were similar in the five experimental groups, food efficiency ratio was higher in the high-fat diet groups(2, 3, 4, and 5) than in the NC group. In evaluating the hematological parameters of the rats, the neutrophil and monocyte ratios were higher in the HC-HSBG, HC-LSBG, and HC-Lov groups than in the HC group. Serum lipid profiles were analyzed after a 12 hr fast at the end of the study. Total cholesterol, triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels were significantly lower in the HC-HSBG and HC-LSBG groups than in the HC group. These results suggest that chemically engineered sulfated mushroom ${\beta}$-glucan (SBG) might contribute to lower cholesterol and lipid levels in blood.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.251-261
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• 2003

As a part of an on-going project investigating flux of materials in Gomso Tidal Flat, we have monitored temporal and spatial distribution of nitrogen components(TN, PON, DON, DIN) and have sought the relationships with the freshwater input(tidal range, salinity), the biological activities(chlorophyll-${\alpha}$, TP, DIP, silicate) and the resuspended bottom sediment in seawater(SPM) from 1999 to 2000. TN in seawater was 39.05 $\mu\textrm{m}$ol 1$\^$-1/ (31.03∼42.93 $\mu\textrm{m}$ol 1$\^$-1/) without any statistical difference(p<0.05) between the studied periods. Organic nitrogen (DON and PON) occupied 75％, 95％, 73％, and 75％ in April, August, September and November, respectively. DON and PON have been found within the narrow concentration ranges of 11.30∼16.38 $\mu\textrm{m}$ol 1$\^$-1/ and 13.16∼20.04 $\mu\textrm{m}$ol 1$\^$-1/ in spite of severe environmental differences through the studied periods. Dissolved fractions of nitrogen(DON and DIN) occupied 53∼65％ of TN. Only DIN varied with an evident temporal variability: low concentrations(1.325∼1.616 $\mu\textrm{m}$ol 1$\^$-1/) in August and high enrichment(8.377∼14.65 $\mu\textrm{m}$ol 1$\^$-1/) in September. High consumption rate of DIN by phytoplankton and a long-lasted drought probably induced such low concentration of DIN in August. Eventually heavy precipitation probably introduced plenty of new nitrogen sources into Gomso Bay in September. The portion of PON, DON and DIN in the total nitrogen was 40％, 38％ and 22％, respectively. Their contents were in the order of DON>PON>DIN for the year round except PON>DON>DIN only in September. The highest DON portion in August probably due to the active microbial decomposition of organic material in summer. Only in April, some evident negative correlations have been found between chlorophyll-${\alpha}$ and DIN mostly nitrate(-0.64, p<0.01), phosphate(-0.46, p<0.01) and silicate(-0.55, p<0.01). The Si(OH)$_4$/DIN/DIP ratios in the water column suggests the limitation of DIN for the growth of phytoplankton during the dry summer in Gomso Bay, which was the case of August in this work. Even with some difference between the studied periods, the primary factors on the distribution of nitrogen components in seawater overlying the Gomso Tidal Flat have been the tidal range and the freshwater input, but the additional variations were due to the biological activities.

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.367-373
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• 2009

This experiment was conducted to determine optimum dietary levels of dietary energy and protein for laying performance and egg quality in organic laying hens. In a $2{\times}3$ factorial arrangement, 360 Hy-Line pullets (21 wk of age) were randomly assigned to experimental diets with 2,800, 3,080, and 3,360 kcal of ME/kg of diet, each containing 16 and 18% CP, respectively. Each dietary treatment was replicated 4 times, and feed and water were provided ad libitum. Experimental birds were raised in floor and received 14h of light throughout 52 weeks of experimental period. The birds were observed for feed consumption, egg production, egg weight, egg mass, feed conversion ratio, Haugh unit, shell thickness, and shell color at 30, 50, and 70 wk of age. Egg production, egg weight, and egg mass were higher (P<0.05) in hens receiving diets with 2,800 kcal/kg of ME/kg of feed than those fed diets containing 3,080 and 3,360 kcal of ME/kg of diets. Eggshell breaking strengths were not significantly different among the dietary ME and CP concentrations. Eggshell thickness was higher (P<0.05) in hens receiving diets with 3,360 kcal of ME/kg of feed than those fed diets containing 2,800 and 3,080 kcal of ME/kg of diets at 50 and 70 week. With increasing in dietary energy levels, the concentrations of blood total cholesterol were also significantly increased. Overall, the laying hens fed organic diet of 2,800 kcal ME/kg and 16% CP showed superior egg production than those of other dietary regimens.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.273-281
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• 2004

A feeding trial was conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the performance, nutrient availability and fatty acid composition of breast muscle was studied. In experiment I, two hundred ten hatched broiler chicks (Ross) were assigned to seven dietary treatments for 5 weeks. Each treatment consisted of 3 replications with 10 birds each. Control diet was formulated to have $22\%$ CP and 3,150 kcal ME/kg for starter diet, $19\%$ CP and 3,200 kcal ME/kg for finisher diet. Euglena gracilis Z. (EG) was added to control diet at the plevel of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, two hundred fifty hatched broiler chicks (Ross) were assigned to five dietary treatments: T1; Control, T2; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T3; T1 + EGD $1.0\%$, T4; T1 + EGBD $0.5\%$, T5; T1 + EGBD $1.0\%$. The weight gain and feed consumption were measured weekly. Fatty acid composition of breast muscle was determined. In experiments I and 2, Euglena supplementation had no significant effects on weight gain, feed intake and feed conversion ratio. In experiment 1, EGBD treatments significantly increased DHA concentration but decreased concentration of linoleic acid and arachidonic acid in breast muscle. EGBD 2% treatment showed the highest DHA concentration (14.27%) which is 3.9 times of that of the control ($3.66\%$). In experiment 2, $1.0\%$ EGBD treatment showed highest EPA, lignoceric acid and DHA level in breast muscle (P<0.05). Also, EGD treatments significantly increased DHA and EPA concentration. It was concluded that EGBD and EGD can be supplemented to broiler diet to produce DHA enriched broiler meat.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.593-600
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• 1996

To investigate the dining habits in relation to Kimchi for the Koreans in Commonwealth of Independent States(CIS), a survey was conducted to 199 Koreans living in Moscow, Sahalin, Uzbekistan, and Kazakstan. Most of the respondents answered that thor keep the Korean food lifestyle, and especially the old age group and Koreans in Sahalin showed higher ratio for Korean food lifestyle. In the Kimchi preference and Kimchi consumption, 85.9% of the respondents like Kimchi because Kimchi is our traditional food and its taste is good, and 95% of them consumed Kimchi once or more everyday. In the kinds of Kimchi, 99% of the respondents had experiences in eating or seeing Chinese cabbage Kimchi, 91.5% cucumber Kimchi, 88.4% cabbage Kimchi, 82.4% white water Kimchi, 75.4% sikhae, respectively, while the frequencies of kakdugi, chonggak Kimchi, and leek Kimchi were relatively low. In connection with the utilization of Kimchi, many respondents answered that Kimchi is sometimes used in cooking of Kimchi cchigae or Kimchi bokkum. Most of Russians like Kimchi in spite of the hot taste and it shows the possibility that Kimchi can be spread throughout foreign countries.

• The Journal of Korean Society for School & Community Health Education
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• v.3
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• pp.79-110
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• 2002

The purpose of this study was to provide basic data necessary for creating a more successful health enhancement program, health education and health policy which can enhance health management abilities and enable a healthy school life by examining relations between high school students' health perception, who are in early adolescence, and their health promotion behavior. The subjects in this study were 813 students from 8 high schools on Jeju Island. The collected data were analyzed with t-test and one-way ANOVA through questionnaires by researcher on the basis of advance research. The findings of this study were as belows : 1. When general characteristics were considered, health perception was more significantly affected by those who were boys, whose family income was on a higher level, who scored better in school, whose parents were more concerned about their children's health, and who had experiences of being hospitalized. Especially, the more parents were concerned about their children's health, the higher the child's self-confidence on health(p<.05), anxiety on health(p<.05), practicality on disease prevention(p<.05), self-confidence on future health(p<.01), health care ability(p<.01), and family roles on health(p<.05). The less parents were concerned about their children's health, the higher the child's anxiety on disease(p<.01) and uncertainty on future health(p<.01). 2. When students' health promotion behavior was concerned, in the area of Eating Habits, having regular meals(72.9%), moderate consumption of meat(89.7%) and a balanced diet(64%) were common, whereas having a regular breakfast(37.4%) and eating vegetables and fruits(43.6%) were rare. In the area of Exercise, all subareas such as exercising 3 or 4 times a week(76.7%), more than one hour of exercise at a time(74.9%), a short distance walk(51.8%), exercise within one hour after meals(87%), and whether wanning up or not(88.6%) were above average. In the area of Personal Hygiene, all subareas such as drinking boiled water(57.3%), washing hands after using the bathroom(71%), brushing teeth after eating(42.4%), washing up after going out(50.3%), and washing hair and taking a frequent bath(77.2%) were rare. In the area of Control of Personal Relations, consulting personal problems with family(78.7%) had a positive response, whereas meeting someone new(59.8%), maintaining sound relations with the opposite sex(47.3%), having respect for one's opinion(51.5%), and maintaining peaceful relations with people(58.4%) had rare negative results. In the area of Stress Management, not being competitive(69.4%), releasing problems(62.4%) and sleeping soundly(66.2%) were common, whereas having your own ways to relieve stress(46.8%) and having your own dreams and hopes(58.5%) were rare. 3. When general characteristics were considered, as far as relations of health promotion behavior were concerned, the boys were significantly different in the area of Eating Habits(p<0.01) and Exercise(p<0.01), and the girls were in the area of Personal Hygiene. As family economic status was high and parents were concerned about their children's health, the entire areas of health promotion behavior were significantly different. Whether or not they were hospitalized made no significant difference in statistics. 4. When their regions were considered, it comparing city to town, there was no statistically significant difference in health perception and relations of health promotion behavior according to general characteristics. As seen in the results above, high school students' level of health perception and level of practicing health promotion behavior were generally low. In conclusion, by enhancing health perception, a plan for activating health education, which can implement positive health promotion behavior, should be made.

• Journal of Environmental Impact Assessment
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• v.25 no.6
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• pp.414-431
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• 2016

There are numerous closed and abandoned mines in Korea, from which diverse heavy metals (e.g., As, Cd, Cu, Pb, Zn) are released into the surrounding soil, groundwater, surface water, and crops, potentially resulting in detrimental effects on the health of nearby residents. Therefore, we performed human risk assessments of two abandoned metal mines, Yanggok (YG) and Samsanjeil (SJ). The exposure parameters used in this assessment were specific to residents near mines and the included exposure pathways were relevant to areas around metal mines. The computed total excess carcinogenic risks for both areas exceeded the acceptable carcinogenic risk ($1{\times}10^{-6}$), indicating that these areas are likely unsafe due to a carcinogenic hazard. In contrast, the non-carcinogenic risks of the two areas differed among the studied receptors. The hazard indices were higher than the unit risk (=1.0) for male and female adults in YG and male adults in SJ, suggesting that there are non-carcinogenic risks for these groups in the study areas. However, the hazard indices for children in YG and female adults and children in SJ were lower than the unit risk. Consumption of groundwater and crops grown in the area were identified as major exposure pathways for carcinogenic and non-carcinogenic hazards in both areas. Finally, the dominant metals contributing to carcinogenic and non-carcinogenic risks were As and As, Cu, and Pb, respectively. In addition, the carcinogenic and non-carcinogenic risks of YG were evaluated to be 10 and 4 times higher than those of SJ, respectively, resulted from the relatively higher exposure concentration of As in groundwater within SJ area. Because of lacking of several exposure parameters, some of average daily dose (ADD) could not be computed in this study. Furthermore, it is likely that the ADDs of crop-intake pathway included some errors because they were calculated using soil exposure concentrations and bioconcentration factor (BCF) rather than using crop exposure concentrations.

• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.319-326
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• 1999

Recently, it is continuously rising to concern about the health risk being induced by microorganisms in food such as Escherichia coli O157:H7 and Listeria monocytogenes. Various organizations and regulatory agencies including U.S.FPA, U.S.DA and FAO/WHO are preparing the methodology building to apply microbial quantitative risk assessment to risk-based food safety program. Microbial risks are primarily the result of single exposure and its health impacts are immediate and serious. Therefore, the methodology of risk assessment differs from that of chemical risk assessment. Microbial quantitative risk assessment consists of tow steps; hazard identification, exposure assessment, dose-response assessment and risk characterization. Hazard identification is accomplished by observing and defining the types of adverse health effects in humans associated with exposure to foodborne agents. Epidemiological evidence which links the various disease with the particular exposure route is an important component of this identification. Exposure assessment includes the quantification of microbial exposure regarding the dynamics of microbial growth in food processing, transport, packaging and specific time-temperature conditions at various points from animal production to consumption. Dose-response assessment is the process characterizing dose-response correlation between microbial exposure and disease incidence. Unlike chemical carcinogens, the dose-response assessment for microbial pathogens has not focused on animal models for extrapolation to humans. Risk characterization links the exposure assessment and dose-response assessment and involve uncertainty analysis. The methodology of microbial dose-response assessment is classified as nonthreshold and thresh-old approach. The nonthreshold model have assumption that one organism is capable of producing an infection if it arrives at an appropriate site and organism have independence. Recently, the Exponential, Beta-poission, Gompertz, and Gamma-weibull models are using as nonthreshold model. The Log-normal and Log-logistic models are using as threshold model. The threshold has the assumption that a toxicant is produce by interaction of organisms. In this study, it was reviewed detailed process including risk value using model parameter and microbial exposure dose. Also this study suggested model application methodology in field of exposure assessment using assumed food microbial data(NaCl, water activity, temperature, pH, etc.) and the commercially used Food MicroModel. We recognized that human volunteer data to the healthy man are preferred rather than epidemiological data fur obtaining exact dose-response data. But, the foreign agencies are studying the characterization of correlation between human and animal. For the comparison of differences to the population sensitivity: it must be executed domestic study such as the establishment of dose-response data to the Korean volunteer by each microbial and microbial exposure assessment in food.

• Journal of Nutrition and Health
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• v.41 no.5
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• pp.391-401
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• 2008

Probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including IBD. However, few probiotics have undergone appropriate preclinical screening in vivo. Kefir is considered a probiotic, benefiting the host through its effects in the intestinal tract. Despite numerous studies examining the action of probiotics on the host organism, few have analyzed the effects on intestinal environment. We assessed the protective effect of kefir for three weeks before inducing colitis with 2% dextran sodium sulfate for five days. The DSS loads were similar in all DSS treatment group. The results of the experiment are as follows. Food intake and FER of experimental groups were not significantly different each other, but water consumption tended to be higher in all DSS treatment groups as compared with the normal control. And visual inspection of feces revealed mild diarrhea in rat given 2% DSS. The anti-inflammatory activity of kefir was determined by myeloperoxidase activity during the DSS treatment, and there was no significant difference in any group. The levels of thiobarbituric acid reactive substances (TBARS) as a colonic lipid peroxidation were significantly lower in the kefir intake groups than in rats treated with 2% DSS alone. The DNA % in tail and tail moment values as a DNA damage level of the blood lymphocytes in kefir intake groups tended to be lower than 2% DSS treatment alone, especially tail lengths were significantly diminished. According to the colonic histopathological assay, there were a severe inflammation of lamina propria and submucosa and mild edema in mucosa and sub mucosa in DSS alone treated group. We found a slight regenerative change in kefir treatment groups. In our experiments, this means that ulcerative colitis related to oxidative injury might be prevented by kefir as a probiotic. Further studies of the potential benefits of kefir as a probiotic in inflammatory condition are encouraged.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.6
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• pp.16-24
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• 2015

This study quantified storage and annual uptake of carbon for riparian greenspaces established in watersheds of four major rivers in South Korea and explored desirable strategies to improve carbon reduction effects of riparian greenspaces. Greenspace structure and planting technique in the 40 study sites sampled were represented by single-layered planting of small trees in low density, with stem diameter at breast height of $6.9{\pm}0.2cm$ and planting density of $10.4{\pm}0.8trees/100m^2$ on average. Storage and annual uptake of carbon per unit area by planted trees averaged $8.2{\pm}0.5t/ha$ and $1.7{\pm}0.1t/ha/yr$, respectively, increasing as planting density got higher. Mean organic matter and carbon storage in soils were $1.4{\pm}0.1%$ and $26.4{\pm}1.5t/ha$, respectively. Planted trees and soils per ha stored the amount of carbon emitted from gasoline consumption of about 61 kL, and the trees per ha annually offset carbon emissions from gasoline use of about 3 kL. These carbon reduction effects are associated with tree growth over five years to fewer than 10 years after planting, and predicted to become much greater as the planted trees grow. This study simulated changes in annual carbon uptake by tree growth over future 30 years for typical planting models selected as different from the planting technique in the study sites. The simulation revealed that cumulative annual carbon uptake for a multilayered and grouped ecological planting model with both larger tree size and higher planting density was approximately 1.9 times greater 10 years after planting and 1.5 times greater 30 years after than that in the study sites. Strategies to improve carbon reduction effects of riparian greenspaces suggest multilayered and grouped planting mixed with relatively large trees, middle/high density planting of native species mixed with fast-growing trees, and securing the soil environment favorable for normal growth of planting tree species. The research findings are expected to be useful as practical guidelines to improve the role of a carbon uptake source, in addition to water quality conservation and wildlife inhabitation, in implementing riparian greenspace projects under the beginning stage.