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pH 자극이 소 정자의 첨모반응에 미치는 영향 (Effect of pH Stimulation on Acrosome Reaction of Bovine Spermatozoa)

  • 박영식;임경순
    • 한국가축번식학회지
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    • 제15권3호
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    • pp.195-199
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    • 1991
  • This study was carried out ot investigate effect of pH stimulation on acrosome reaction of bovine spermatozoa. The results obtained were as follows : 1. When sperm was sequentially washed with SHP solution of pH 7.4, 7.7 and 7.4 and incubated in mTALP solution of pH 7.4 for 120min, 15, 30, 60 and 120min incubations showed significantly(p<0.05) higher sperm acrosome reaction rate than 0 min. 2. When sperm was sequentially washed with SHP solution of pH 7.4, 8.0 and 7.4 and incubated in mTALP solution of pH 7.4 for 15 minutes, sperm acrosome reaction rate was significantly(p<0.01) increased until 9 min. Incubation, but not increased thereafter. 3. When sperm were separately washed with SHP solutions of pH 7.0, 7.4 and 8.0 and incubated in mTALP solution of pH 7.4 for 9min, sperm acrosome reaction rate was 74.8, 71.8 and 93.4%. pH 8.0 showed signifciantly(p<0.01) higher sperm acrosome reaction rate than pH 7.0 and 7.4. The results suggest that stimulation of sperm with high pH induces sperm crosome reaction.

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pH가 glass ionomer cement의 불소이온 용출량과 용해성에 미치는 영향에 관한 실험적 연구 (THE EFFECT OF pH MEDIA ON THE FLUORIAE RELEASE AND SOLUBILITY OF GLASS IONOMER CEMENT)

  • 이광희;최호영
    • Restorative Dentistry and Endodontics
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    • 제15권1호
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    • pp.88-96
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    • 1990
  • The purpose of this study was to investigate the fluoride release and solubility of glass ionomer cement associated with three pH media. For this study, GC Fuji II discs (20.0mm in diameter ${\times}$ 1.5mm thick) were immersed in pH 4.0 lactic acid, pH 7.0 distilled water and pH 10.0 KOH solutions for 1, 7, 14 and 28 days. The amount of fluoride release from the cement into three pH media were measured by fluoride specific ion electrode and the solubility was measured by weight loss of discs. The results were as follows: 1. The lower was the pH of media, the more was the amount of release of fluoride. 2. The amount of fluoride release was increased with time lapse. 3. After I day, the solubility was the highest, and after 7 days that was the least. 4. The lower was the pH of media, the more was the solubility, but there was no statistical difference in solubility according to the pH change.

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유치원 아동을 둔 어머니들의 영양교육 프로그램의 효과 평가 (Effect-Evaluation of Nutrition Education Program for the Mothers of Preschool Children in Kindergarten)

  • 강현주;김경미;김경자;류은순
    • Journal of Nutrition and Health
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    • 제34권2호
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    • pp.230-240
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    • 2001
  • This study was designed to develop the nutrition education program for the mothers of preschool children in kindergarten and evaluate its educational effects. Nutrition education program was developed on the basis of the concept which consisted of nutrition, nutrients, menu planning, cooking, recognition of nutrition education, food selection and menu planning, will of nutrition improvement. Subjects consisted of 41 mothers. All the subjects completed a pretest and a posttest. The nutrition knowledge score of pretest was 33.77$\pm$12.53 and that of posttest was 55.25$\pm$16.32 and the difference was significant(p<0.001). The Food attitude score of pretest was 66.40$\pm$6.26 and that of posttest was 70.76$\pm$6.05 and the difference was significant(p<0.001). Food attitude score of high score-group of nutrition knowledge was higher than that of low score-group of nutrition knowledge significantly, in pretest(p<0.001) and posttest(p<0.01) respectively. Nutrition knowledge score and food attitude score of children of educated parents of nutrition program was higher than that of children of uneducated parents of nutrition program but the difference was not significant. The nutrition densities of vitamin A(p<0.001), vitamin B$_1$(p<0.05), vitamin B$_2$(p<0.001), folic acid(p<0.05), Ca(p<0.001), p(p<0.001), calculated using the INQ(Index of Nutritional Quality), was significantly improved by nutrition consult and education program. The MAR(Mean Adequacy Ratio) of pretest was 0.79$\pm$0.23 and that of posttest was 0.91$\pm$0.16 and the difference was significant(p<0.05). (Korean J Nutrition 34(2) : 230-240, 2001)

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추출 pH가 분리대두단백질의 기능성에 미치는 영향 (Influences of Extraction pH on the Functionality of Soybean Protein Isolate)

  • 배송환;이철
    • 한국식품과학회지
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    • 제30권3호
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    • pp.557-561
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    • 1998
  • 본 연구에서는 산성(pH 2.0, 3.0), 중성(pH 7.0) 및 알칼리성(pH 10.0, 12.0)영역에서 각각 추출된 SPI들의 기능성을 상호비교하였다. pH 3.0에서 추출된 SPI의 단백질함량은 93.31%로 최고값을, pH 7.0에서 추출된 SPI의 단백질함량은 73.93%로 최소값을 나타내었다. pH 3.0에서 추출된 SPI의 추출수율은 0.36%로 최소값을, pH 12.0에서 추출된 SPI의 추출수율은 47.54%로 최고값을 나타내었다. SPI의 기능성(용해도, 수분흡수력, 유지흡수력, 기포형성력, 기포안정성, 유화형성력 및 겔형성력)은 대두단백질 추출시 추출용액의 pH에 의해 크게 좌우되었다. pH 2.0과 3.0에서 추출된 SPI의 용해도는 산성영역에서 크게 나타났고 pH 3.0과 7.0에서 추출된 SPI는 중성 영역에서 높은 용해도를 보였으나 pH 2.0, 3.0, 7.0, 10.0 및 12.0에서 추출된 SPI는 모두 알칼리성 영역(pH 9.0-10.0)에서 높은 용해도를 나타내었다. pH 2.0과 pH 12.0에서 추출된 SPI의 수분흡수력, 유지흡수력, 기포형성력 및 기포안정성은 다른 pH에서 추출된 SPI보다 크게 나타났으나 유화력의 경우 추출용액의 pH가 증가할수록 유화력이 증가하였다.

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습식법으로 제조한 수산화아파타이트 침전입자와 분말의 특성에 미치는 pH변화의 영향 (Effect of pH Variation on the Properties of the Hydroxyapatite Precipitates and Powder Prepared by the Wet Method)

  • 신용규;정형진;김병호
    • 한국세라믹학회지
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    • 제26권2호
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    • pp.157-166
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    • 1989
  • The effect of pH variation in starting solution on the characteristics of hydroxyapatite precipitates and powder prepared by the wet method was investigated. Hydroxyapatite precipitates was agglomerated, the average agglomerated particle size was decreased in the range from 2 to 6${\mu}{\textrm}{m}$ with increasing pH values in starting solution. The aspect ratio of rod-shaped hydroxyapatite particle was rapidly increased at pH 11 and 11.5. The maximum specific surface area, 91.1$m^2$/g, was at pH 10.5. Dried powder prepared at high pH values contained more minutes CO2 than that prepared at low pH values. The poor crystallinity was maintained up to $600^{\circ}C$ regardless of the pH values in starting solutions. However, the second phase or high crsytalline hydroxypatite phase appeared above 80$0^{\circ}C$. In pH 9.5 and pH 10, $\beta$-whitlockite transformed to $\alpha$-whitlockite at 120$0^{\circ}C$, while in pH 10.5-11.5, hydroxyapatite phase was maintained up to 120$0^{\circ}C$.

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Comparison of Total Protein, DNA, and RNA Contents by Corpus Luteum in Various Stages of Estrous Cycle and Pregnancy

  • K. S. Baek;Kim, Y. S.;Lee, C. N.
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2001년도 춘계학술발표대회
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    • pp.79-79
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    • 2001
  • This study was conducted to measure the total protein, DNA, and RNA contents of corpus luteum(CL) in various stages of estrous cycle and pregnancy. CLs were collected from a local slaughterhouse and stages of the estrous cycle of CL were classified as CL1~2, days 1 to 10; CL3(with/without central cavity), days 11 to 17; CL4, days 18 to 20 by method of Ireland et. al(1980) and stages of the pregnancy of CL were classified as P1~3, months 11~3: P4~6, months 4~6; P7~9, months 7~9 of pregnancy. CL3 with/without central cavity(CC) was identified as described by Kastelic et. al.(1990)-CL with CC, more than 2mm in diameter; CL without CC, less than 2mm in diameter. In total protein content, CL3 with CC was significantly lower than P7~9(p<.05). The total DNA content was lower in CL3 with CC than CL3 without CC and CL4(p<.05). In protein : DNA ratio, CL3 with CC was significantly lower than CL4(p<.05), CL3 without CC was significantly lower than P7~9(p<.05), CL4 was significantly lower than CL3 with CC, P1~3 and P7~9(p<.05). No differences were observed in RNA content, protein:RNA ratio, RNA/DNA of CLs in stages of estrous cycle and pregnancy.

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익산지역 강수의 계절별 산성도와 화학성상 (Seasonal Variations of Acdity and Chemicstry of Precipitation in Iksan Area)

  • 강공언;오인교;김희강
    • 한국대기환경학회지
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    • 제15권4호
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    • pp.393-402
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    • 1999
  • Precipitation samples were collected by the wet-only sampling method in Iksan in the northwest of Chonbuk from March 1995 to February 1997. These samples were analyzed for the concentration of ion components, in addition to pH and electrical conductivity. The annual mean pH of precipitation was 4.8 and the seasonal trend of pH was shown to be low in Fall and Winter(4.5), middle-ranged in Spring(4.7) and high in Summer(5.0). The frequency of pH below 5.6 was about 71%. The seasonal pattern of pH frequency was found to be different in each season. In the case of the pH less than 5.0, the frequency was higher in Spring, Fall and Winter than in Summer, especially higher in Fall than in other seasons. The concentrations of analysed ions showed a pronounced seasonal pattern. However, major ion species for all seasons were $NH^+_4,;Ca^{2+};and;Na^+$ among cations and $SO^{2-}_4,;Cl^-;and;NO^-_3$ among anions. The major acidifying species appeared to be $nss-SO^{2-}_4;and;NO^-_3$, and the main bases responsible for the neutralization of precipitation acidity were $nss-Ca^{2+};and;NH^+_4$. The potential acidity of precipitation, pAi, was found to be between 3.0 and 5.0 for total samples, while the measured pH was approximately between 3.9 and 7.8. The seasonal trend of pAi showed a decreasing order: Summer (4.3), Winter(4.0), Spring and Fall(3.8). During the Fall, both pAi and pH were especially very low, which indicated that during this period the potential acidity of precipitation was high but the neutralizing capacity was low. For Spring, pAi was very low but pH was slightly high. This was likely due to the large amount of $CaCO_3$ in the soil particles transported over a long range from the Chinese continent that were incorporated into the precipitation, and then neutralized the acidifying species with its high concentraton.

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간호사의 노인의 성에 대한 지식, 태도 및 성교육 요구도 (Nurses' Knowledge, Attitude and Education Needs for Sexuality of the Elderly)

  • 양정임;정미현
    • 한국콘텐츠학회논문지
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    • 제13권3호
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    • pp.260-270
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    • 2013
  • 본 연구는 간호사의 노인의 성에 대한 지식, 태도, 교육 요구도를 파악하기 위한 서술적 조사연구이다. 연구 대상자는 전라북도의 I, K, J시의 종합병원 2곳과 노인 요양원(병원) 5곳에 근무하는 간호사 235명이었으며 자가보고 설문지를 이용하여 2012년 12월 1일부터 10일까지 자료 수집을 하였다. 자료 분석은 SPSS win 18.0프로그램을 이용하였다. 각 변수는 t-test, ANOVA, Pearson's correlation coefficients and DUNCAN을 사용하였다. 연구결과, 대상자의 성지식 평균점수는 $15.91{\pm}6.43$이고, 결혼(p=.024), 근무지(p<.001), 경력( p=.006), 성교육 필요여부(p<.001), 성교육 수용여부(p<.001) 등이 통계적으로 유의하였다. 성태도 평균점수는 $38.26{\pm}4.41$이고 근무지(p=.033), 노인 가족과 동거여부(p=.009), 성교육 필요여부(p<.001), 성교육 수용여부(p<.001)등이 통계적으로 유의하였다. 성교육 요구도의 평균점수는 $35.97{\pm}6.28$이며 연령(p=.023), 노인 가족과 동거여부(p=.014), 성교육 필요여부(p<.001), 성교육 수용여부(p<.001) 등이 통계적으로 유의하였다. 간호사의 노인의 성에 대한 성지식은 성태도(p<.001)와 성교육 요구도(p<.001))가 양의 상관관계로 나타났다. 이 연구 결과로 학부과정 및 간호사 대상으로 노인의 성에 관한 전반적인 교육의 필요성과 중요성을 알 수 있었으며, 이를 기초로 임상에서 노인들의 건강 사정 및 간호 중재 시 노인의 성에 대해 좀 더 적극적인 간호가 요구된다.

Reduction of Phosphate Adsorption by Ion Competition with Silicate in Soil

  • Lee, Yong-Bok;Kim, Pil-Joo
    • 한국환경농학회지
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    • 제26권4호
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    • pp.286-296
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    • 2007
  • To increase phosphate (P) availability in soils, the efficiency of silicate (Si) in reducing P adsorption was investigated by competitive adsorption tests under changing conditions of pH, ion concentrations, and order of anion addition along with single adsorption properties of each ion at $20^{\circ}C$. In the single ion adsorption study, P and Si ions showed the opposite reaction patterns: phosphate adsorption decreased with increasing pH and attained adsorption maximum however, silicate adsorption increased with increasing pH without attaining adsorption maximum. Phosphorus and Si adsorption were influenced by pH in the range of 5.0 - 9.0 and the type and amount of P and Si concentration. Silicate added to soil before P or in a mixture with P significantly reduced P adsorption above pH 7.0; however, there was no significant Si-induced decreased in P adsorption at pH 5.0 when anions were added as mixture. The efficiency of Si in reducing P adsorption increased with increasing Si concentration and pH. The effect of P on Si adsorption was relatively small at pH 5.0 and no effect of P on silicate adsorption was observed at pH 9.0. The presence of Si strongly depressed P adsorption when Si was added before P compared to P and Si added as a mixture. These results suggest that application of Si may decrease P adsorption and increase the availability of P in soils. Furthermore, a Si source would be better to add before P application to enhance the availability of P in soils.

P4VP과 PDP로 이루어진 Molecular Bottle-brush의 Order-Disorder Transition에 미치는 P4VP 분자량의 영향에 관한 연구 (Effects of Molecular Weight of Poly(4-vinylpyridine) on the Order-Disorder Transition of Molecular Bottle-brush Composed of Poly(4-vinylpyridine) and 3-Pentadecylphenol)

  • 최종렬;조항규;전현애;노시태
    • 폴리머
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    • 제24권4호
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    • pp.488-498
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    • 2000
  • 주쇄로 poly(4-vinylpyridine) (P4VP)과 양친매성 측쇄로 3-pentadecylphenol (PDP)의 수소결합을 통해서 molecular bottle-brush를 제조하였다. 제조된 bottle-brush에 대하여 P4VP의 pyridine기 대 PDP의 몰비 (x)와 P4VP 분자량에 따른 액정성의 구조, 상전이온도( $T_{ODT}$)와 bottle-brush 층간거리 ( $L_{p}$)변화에 대하여 고찰하였다. P4VP-PD $P_{x}$ bottle-brush는 상온영역에서 미세상분리를 이루고 있는 라멜라구조로 조사되었다. Bottle-brush의 온도에 따른 상거동에 대한 조사에서 상한임계온도(UCST)거동을 나타내었다. 또한 P4VP의 분자량별로 x가 0.8-0.9일때 최대온도를 나타내었으며, P4VP의 분자량이 증가함에 따라서 상전이가 높은 온도에서 일어났다. 이러한 결과는 주쇄긴 P4VP의 유동성과 라멜라구조의 크기와 규칙성에 영향을 받음을 알 수 있었다. Bottle-brush의 라멜라구조 분석시 bottle-brush의 $L_{p}$는 35 $\AA$에서 40 $\AA$로 x보다는 P4VP의 분자량에 더 큰 영향을 받았다. P4VP의 분자량이 증가함에 따라서 $L_{p}$도 증가하였으나, 일정 크기이상으로 분자량이 커졌을 경우, 오히려 거리가 감소하거나 증가하지 않는 결과를 얻었다.결과를 얻었다.다.

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