• Journal of Animal Science and Technology
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• v.53 no.3
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• pp.261-268
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• 2011

The increasing demand of the crops (soybean and corn) for biofuel production has increased the focus of the animal nutritionists to look for alternative feeds, which are economic and environmental friendly. To identify microalgae as suitable candidate as an alternative feed, growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (0.07, 1.4, 3.0 and 5.0%) and photon densities (39.19, 72.97, 105.41, 116.22, 135.14, $175.68\;{\mu}mol/m^2/s$) by employing a photo-bioreactor. Swine wastewater was also investigated as nutritional source to economize the biomass production. Results showed that the higher biomass production was found to be at 3.0% $CO_2$ compared to other $CO_2$ concentrations. However, no difference in biomass production was found at $105.41\;{\mu}mol/m^2/s$ and above photon densities with 12 h of photoperiodicity. It was observed that C. vulgaris could easily grow in 200 times diluted swine wastewater and growth was found to be similar with that of artificial medium. Provided the conducive conditions for optimal growth, it has also the potentiality of depleting ammonia nitrogen ($NH_4$-N) and orthophosphate ($PO_4^{3-}$-P) completely from the wastewater after 3~4 days of cultivation. Thus, growing C. vulgaris would not only solve the problem of animal feed, but also help in biological $CO_2$ mitigation and wastewater treatment.

• Journal of Korean Society of Water and Wastewater
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• v.26 no.6
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• pp.833-840
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• 2012

This study aimed to investigate growth rate and nutrient removal efficiency of Chlorella vulgaris according to nitrogen sources and frequency of pH adjustment. Nitrogen and phosphorus removal efficiencies were evaluated in the three different conditions using $NO_3{^-}$, $NH_4{^+}$ as a sole nitrogen source and mixed condition. Initial nutrient concentrations in artificial wastewater were 30 mg-N/L and 3 mg-P/L similar to secondary wastewater effluent. When nitrogen source was $NO_3{^-}$, there was no inhibition on the growth of C. vulgaris with adjusting pH every 24 hr while growth inhibition occurred with $NH_4{^+}$ caused by pH drop. N, P removal efficiencies were no significant depending on the nitrogen sources. As pH was adjusted to 7 by pH-stat, growth rate and nutrient removal efficiencies were increased compared to adjusting pH every 24 hr, however, growth rate and nutrient removal efficiencies were no significant depending on the nitrogen sources.

• Korean Journal of Medicinal Crop Science
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• v.19 no.6
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• pp.484-490
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• 2011

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging ($IC_{50}=0.50{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p < 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.560-567
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• 1998

We have found that Thermoactinomyces vulgaris KFB-Cl00 produces a chitinase. The optimum temperature and pH of the enzyme activity were $55^{\circ}C$ and 6.5. The enzyme was stable after heat treatment at $80^{\circ}C$ for 30 min and stable in acidic and basic conditions (PH 6.0~11.0). The thermostable endo-chitinase from Thermoactinomyces vulgaris KFB-C100 was cloned into the plasmid pBR322 by using E. coli DH5$\alpha$ as a host strain. The positive clone carrying a recombinant plasmid (PKCHI23) with a 4.1-kb fragment containing the chitinase gene was found. The recombinant plasmid was analyzed to determine the essential region for chitinase activity and obtained a 2.3-kb fragment, which was sub cloned into pTrc99A using the PstI and SalI sites to construct pTrc99A/pKCHI23-3. The resulting plasmid exerted high chitinase activity upon transformation of E. coli XL1-Blue cells. Chitinase was overproduced 14 times more in the clone cells than in the wild-type cells and the enzyme was purified to homogeneity. The purified enzyme showed the similar properties as the native chitinase from T. vulgaris in terms of molecular weight and substrate specificity. The catalytic action of the cloned enzyme was an endo type, producing chitobiose as a major reaction product.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.261-266
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• 2012

Fermented Chlorella vulgaris CBT$^{(R)}$ was evaluated for its effects on egg production, egg quality, liver lipids and intestinal microflora in laying hens. One hundred and eight Hy-line Brown layers (n = 108), 80 wk of age, were fed a basal diet supplemented with CBT$^{(R)}$ at the level of 0, 1,000 or 2,000 mg/kg, respectively for 42 d. Egg production was measured daily and egg quality was measured every two weeks. Five eggs from each replicate were collected randomly to determine egg quality. Egg production increased linearly with increasing levels of CBT$^{(R)}$ supplementation (p<0.05), although there was no significant effect of treatment on feed intake. Egg yolk color (p<0.001) and Haugh unit (p<0.01) improved linearly with increasing dietary CBT$^{(R)}$. Hepatic triacylglycerol level was linearly decreased with increasing dietary CBT$^{(R)}$ (p<0.05). The supplemental CBT$^{(R)}$ resulted in linear (p<0.001) and quadratic (p<0.01) response in population of cecal lactic acid bacteria. In conclusion, fermented Chlorella vulgaris supplemented to laying hen diets improved egg production, egg yolk color, Haugh unit and positively affected the contents of hepatic triacylglycerol and the profiles of cecal microflora.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1420-1426
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• 2019

This study was done to investigate the protective effects of ethanol extract Artemisiae vulgaris L(Av) on carbon tetrachloride(CCl₄)intoxicated rats. Male sprague Dawley rats(200~210g)was used. experimental groups were divided into normal group, CCl₄-control group, and ethanol extract CCl₄-treated group. CCl₄-treated groups were injected with CCl₄ 0.6mg/kg.b.w(i.p). The activities of Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Alkaline phosphatase(ALP), Glutamyl transpeptidase(γ-GT), Lactate dehydrogenase(LDH) in extract pretrated group was significantly decreased(p<0.05) compared to the CCl₄-control group. The contents of triglyceride, cholesterol and lipid peroxide were significantly decreased(p<0.05). whereas the contents of HDL-cholesterol and glutathione(GSH) were significantly increased(p<0.05). These results suggest that extract of Artemisiae vulgaris L(Av) has hepatoprotective effect in the CCl₄-intoxicated rats.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.2
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• pp.91-97
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• 2005

The purpose of this research is malodor reduction of food waste leachate by some microorganism. In oder to observe the efficiency of malodor reduction by different microorganisms, 300ml of food waste leachate was fermented by the addition of 10% precultured seed microorganism such as marine algae of Spirulina plantensis, Chlorella vulgaris, the yeast Saccharomyces cerevisae and bacteria Bacillus cereus for 10 days. During the fermentation the change in pH, salinity and sensory evaluations were tested. As the results, the pH values of samples in the beginning were 3.5~4.0. After fermentation they are increased to the level 4.9~7.4. The salinity values of the samples fermented by Spirulina plantensis and Chlorella vulgaris were lowered rapidly. By the sensory evaluation, the efficiency of malodor reduction by Chlorella vulgaris of the marine microalgae showed almost inodorous degree of ordor 1.

• Journal of Environmental Science International
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• v.22 no.6
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• pp.705-715
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• 2013

The response of the freshwater microalga Chlorella vulgaris to mercuric ion ($Hg^{2+}$) stress was examined using chlorophyll a fluorescence image analysis and O-J-I-P analysis as a way to monitor the toxic effects of mercury on water ecosystems. The levels of photosynthetic pigments, such as chlorophyll a and b and carotenoids, decreased with increasing $Hg^{2+}$ concentration. The maximum photochemical efficiency of photosystem II(Fv/Fm) changed remarkably with increasing $Hg^{2+}$ concentration and treatment time. In particular, above $200{\mu}M\;Hg^{2+}$, considerable mercury toxicity was seen within 2 h. The chlorophyll a fluorescence transient O-J-I-P was also remarkably affected by $Hg^{2+}$; the fluorescence emission decreased considerably in steps J, I, and P with an increase in $Hg^{2+}$ concentration when treated for 4 h. Subsequently, the JIP-test parameters (Fm, Fv/Fo, RC/CS, TRo/CS, ETo/CS, ${\Phi}_{PO}$, ${\Psi}_O$ and ${\Phi}_{EO}$) decreased with increasing $Hg^{2+}$ concentration, while N, Sm, ABS/RC, DIo/RC and DIo/CS increased. Therefore, a useful biomarker for investigating mercury stress in water ecosystems, and the parameters Fm, ${\Phi}_{PO}$, ${\Psi}_O$, and RC/CS can be used to monitor the environmental stress in water ecosystems quantitatively.

• Korean Journal of Plant Resources
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• v.24 no.3
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• pp.272-279
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• 2011

It is very crucial to evaluate the genetic diversity of peanut genetic resources for identification of peanut germplasm accessions and variety improvement. Cultivated peanut generally has two subspecies, hypogaea and fastigiata. In this study, we identified peanut into three plant types, virginia (var. hypogaea), spanish (var. vulgaris), and valencia (var. fastigiata). Former one belongs to ssp. hypogaea and latter two are involved in ssp. fastigiata. Twenty SSR markers were used to assess the genetic variation of three sets, hypogaea, vulgaris, and fastigiata, respectively. Out of variety-specific SSR primers tried in this study, ten pairs of SSR primers showed polymorphisms. Each accession could be identified by a specific set of polymorphic SSR primers, and allele number was evaluated among accessions, with an average of 6.7 in var. hypogaea and 5.4 in var. vulgaris and fastigiata. For evaluation of genetic diversity, gene diversity ranged from 0.336 to 0.844 and PIC (polymorphism information contents) ranged from 0.324 to 0.827 were investigated. Dendrograms based on genetic distances were constructed, which showed the existence of three different clusters. And these three different clusters might be associated with the genes involved in three plant types. The results also suggested that there were plentiful SSR polymorphisms among peanut germplasm accessions in RDA (Rural Development Administration, Korea) Genebank and SSRs might play an important role in evaluating peanut accessions and cultivar improvement.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.1
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• pp.123-128
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• 2004

Numerous novel ingredients have been introduced for the higher functionality of whitening cosmetics. Through the preliminary research, we have found Trichosanthes kirilowii root, Prunella vulgaris leaf and Clematis chinensis root have high whitening efficacy. But they are insoluble. Moreover the discoloration of and decrease in content take place when they are exposed to light, heat or oxygen. From Trichosanthes kirilowii root, Prunella vulgaris leaf and Clematis chinensis root, efficacious ingredients were ethanol-extracted by heating to 75∼85$^{\circ}C$ for 6∼8 h. These extracts have the inhibitory activity of tyrosinase and B16 melanin formation, thus enhancing whitening effect. We made liposomes using propylene glycol (PG)/hydrogenated lecithin/middle chain triglycerides (MCT)/glycerin/water and microfuidizer to stabilize extracts. The stability against heat and light was enhanced by 3∼5 times compared with untreated extracts. Particle size analyzer, freeze fracture transmission electron microscopy (FF-TEM), chromameter and HPLC are used for the analysis.