Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Chlorophyll a Fluorescence Response to Mercury Stress in the Freshwater Microalga Chlorella Vulgaris

담수산 클로렐라(Chlorella vulgaris)의 수은 스트레스에 대한 엽록소형광 반응

  • Oh, Soonja (Agricultural Research Center for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Koh, Seok Chan (Department of Biology, Jeju National University)
  • 오순자 (농촌진흥청 국립원예특작과학원 온난화대응농업연구센터) ;
  • 고석찬 (제주대학교 생물학과)
  • Received : 2012.11.15
  • Accepted : 2013.02.25
  • Published : 2013.06.28
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Abstract

The response of the freshwater microalga Chlorella vulgaris to mercuric ion ($Hg^{2+}$) stress was examined using chlorophyll a fluorescence image analysis and O-J-I-P analysis as a way to monitor the toxic effects of mercury on water ecosystems. The levels of photosynthetic pigments, such as chlorophyll a and b and carotenoids, decreased with increasing $Hg^{2+}$ concentration. The maximum photochemical efficiency of photosystem II(Fv/Fm) changed remarkably with increasing $Hg^{2+}$ concentration and treatment time. In particular, above $200{\mu}M\;Hg^{2+}$, considerable mercury toxicity was seen within 2 h. The chlorophyll a fluorescence transient O-J-I-P was also remarkably affected by $Hg^{2+}$; the fluorescence emission decreased considerably in steps J, I, and P with an increase in $Hg^{2+}$ concentration when treated for 4 h. Subsequently, the JIP-test parameters (Fm, Fv/Fo, RC/CS, TRo/CS, ETo/CS, ${\Phi}_{PO}$, ${\Psi}_O$ and ${\Phi}_{EO}$) decreased with increasing $Hg^{2+}$ concentration, while N, Sm, ABS/RC, DIo/RC and DIo/CS increased. Therefore, a useful biomarker for investigating mercury stress in water ecosystems, and the parameters Fm, ${\Phi}_{PO}$, ${\Psi}_O$, and RC/CS can be used to monitor the environmental stress in water ecosystems quantitatively.

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References

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