• Biomedical Science Letters
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• v.26 no.4
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• pp.275-287
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• 2020

Since its introduction in the forensic field, quantitative PCR (qPCR) has played an essential role in DNA analysis. Quality of DNA should be evaluated before short tandem repeat (STR) profiling to obtain reliable results and reduce unnecessary costs. To this end, various human DNA quantification kits have been developed. Among these kits, the PowerQunat® System was designed not only to determine the total amount of human DNA and human male DNA from a forensic evidence item, but also to offer data about degradation of DNA samples. However, a crucial limitation of the PowerQunat® System is its high cost. Therefore, to minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/2-volume) using DNA samples of varying types and concentrations. Our results demonstrated that the low-volume method has almost comparable performance to the manufacturer's method for human DNA quantification, human male DNA quantification, and DNA degradation index. Furthermore, using a reduced volume of regents, it is possible to run 2 times more reactions per kit. We expect the proposed low-volume method to cut costs in half for laboratories dealing with large numbers of DNA samples.

• Journal of Korean Neurosurgical Society
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• v.64 no.4
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• pp.514-523
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• 2021

Objective : Aneurysm volume quantification (AVQ) using the equation of ellipsoid volume is widely used although it is inaccurate. Furthermore, AVQ with 3-dimensional (3D) rendered data has limitations in general use. A novel universal method for AVQ is introduced for any diagnostic modality and application to any shape of aneurysms. Methods : Relevant AVQ studies published from January 1997 to June 2019 were identified to determine common methods of AVQ. The basic idea is to eliminate the normal artery volume from 3D model with the aneurysm. After Digital Imaging and Communications in Medicine (DICOM) data is converted and exported to stereolithography (STL) file format, the 3D STL model is modified to remove the aneurysm and the volume difference between the 3D model with/without the aneurysm is defined as the aneurysm volume. Fifty randomly selected aneurysms from DICOM database were used to validate the different AVQ methods. Results : We reviewed and categorized AVQ methods in 121 studies. Approximately 60% used the ellipsoid method, while 24% used the 3D model. For 50 randomly selected aneurysms, volumes were measured using 3D Slicer, RadiAnt, and ellipsoid method. Using 3D Slicer as the reference, the ratios of mean difference to mean volume obtained by RadiAnt and ellipsoid method were -1.21±7.46% and 4.04±30.54%, respectively. The deviations between RadiAnt and 3D Slicer were small despite of aneurysm shapes, but those of ellipsoid method and 3D Slicer were large. Conclusion : In spite of inaccuracy, ellipsoid method is still mostly used. We propose a novel universal method for AVQ that is valid, low cost, and easy to use.

• Journal of the Korean Society of Combustion
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• v.8 no.4
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• pp.1-8
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• 2003

In this study, the quantification of soot particles in laminar diffusion flame with LII/LIS methods was performed. In these quantification, soot diameter, number density and volume fraction are included. For the quatification of soot particles, calibration tests are needed and the development of algorithm has to be performed. So, in this study, extinction and scattering test at co-flow burner were performed to acquire calibration data. And algorithm for LII/LIS simultaneous measurement for the quantification of soot were developed. The algorithm, which was the quantification of simultaneous photographing using one ICCD camera, to measure LII/LIS signal simultaneously, the best fitted light intensity and acquisition time was needed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.1
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• pp.206-211
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• 2006

The quantification analysis problem is that how the m entities that have n characteristics can be linked to p-dimension space to reflect the similarity of each entity In this paper, the optimization approach for the quantification analysis problem using neural networks is suggested, and the performance is analyzed The computation of average variation volume by mean field theory that is analytical approximated mobility of a molecule system and the annealed mean field neural network approach are applied in this paper for solving the quantification analysis problem. As a result, the suggested approach by a mean field annealing neural network can obtain more optimal solution than the eigen value analysis approach in processing costs.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.4
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• pp.315-323
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• 2013

Tire noise is divided into a road noise(structure-borne noise) and a pattern noise(air-borne noise). Whilst the road noise is caused by the structural vibration of the components on the transfer path from tire to car body, the pattern noise is generated by the air-pumping between tire and road. In this paper, a practical method to estimate the pattern noise inside a passenger car is proposed. The method is developed based on the sound intensity and airborne source quantification. Sound intensity is used for identifying the noise sources of tire. Airborne source quantification is used for estimating the sound pressure level generated by each noise source of a tire. In order to apply the airborne source quantification to the estimation of the sound pressure, the volume velocity of each source should be obtained. It is obtained by using metrics inverse method. The proposed method is successfully applied to the evaluation of the interior noises generated by four types of tires with different pattern each other.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.06a
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• pp.55-56
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• 2009

• Analytical Science and Technology
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• v.35 no.5
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• pp.218-227
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• 2022

A simple and fast throughput flow injection (FI) system with a merging-zone technique was designed to determine ferrous and ferric in an aqueous solution. The method is based on the direct reaction of ferrous with a Bathophenanthroline reagent (Bphen) in acidic media. The forming red complex absorbs light at 533 nm. All conditions of the flow injection system were investigated. The analytical curve of ferrous was linear in the range of 0.07 to 4 mg/L with an r² value of 0.9968. The detection and quantification limits were 0.02 and 0.04 mg/L, respectively. The molar absorptivity and Sandell's sensitivity were 4.0577 × 10⁶ L/mol cm and 25 × 10^-5 ㎍/cm², respectively. The homemade valve was low-cost with high repeatability (n = 7) at an RSD of 1.26 % and zero dead volume. The values of the dispersion coefficient were 2.318, 2.022, and 1.636 for the concentrations of 0.2, 1, and 3 mg/L, respectively. The analysis throughput of the designed flow injection unit was 57 sample per hour.

• Nuclear Engineering and Technology
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• v.55 no.8
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• pp.2823-2834
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• 2023

Austenitic stainless steel welds (ASSWs) of nuclear components undergo aging-related degradations caused by high temperature and neutron radiation. Since irradiation leads to the change of material characteristics, relevant quantification is important for long-term operation, but limitations exist. Although ion irradiation is utilized to emulate neutron irradiation, its penetration depth is too shallow to measure bulk properties. In this study, a systematic approach was suggested to estimate mechanical properties of ion irradiated 308 ASSW. First of all, weld specimens were irradiated by 2 MeV proton to 1 and 10 dpa. Microstructure evolutions due to irradiation in δ-ferrite and austenite phases were characterized and micropillar compression tests were performed. In succession, dislocation density based stress-strain (S-S) relationships and quantification models of irradiation defects were adopted to define phases in finite element analyses. Resultant microscopic S-S curves were compared to verify material parameters. Finally, macroscopic behaviors were calculated by multiscale simulations using real microstructure based representative volume element (RVE). Validity of the approach was verified for the unirradiated specimens such that the estimated S-S curves and 0.2% offset yield strengths (YSs) which was 363.14 MPa were in 10% agreement with test. For irradiated specimens, the estimated YS were 917.41 MPa in 9% agreement.

• Journal of Veterinary Clinics
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• v.25 no.2
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• pp.96-101
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• 2008

This study was performed to quantify the pleural effusion in radiography, ultrasonography and computed tomography(CT) and to evaluate and compare the usefulness of these methods. Normal saline of 10 ml/kg was infused into the pleural space until a final loading volume of 60 ml/kg body weight was reached in six Beagle dogs. The radiographic examination was performed for the detection and quantification of pleural effusion. On the ultrasonographic study, the maximum perpendicular distance was measured between the surface of the lung and the thoracic wall to evaluate pleural effusion. On the CT image, pleural effusion was evaluated as the perpendicular distance to the thoracic surface in the maximum pleural effusion volume on any transverse images with soft tissue window. Statistical analysis was performed using linear regression test. The volume of pleural effusion and measurements of radiography and ultrasonography had no statistical relationship. However, a significant correlation was identified between the volume of pleural effusion and the depth at right ($r^2=0.715$), left ($r^2=0.745$), and mean right and left depth ($r^2=0.844$) on the CT images. All of the thoracic radiographs, ultrasonography, and CT are useful in recognition of pleural effusion. In quantification of pleural effusion, the CT measurement method is superior to radiographic and ultrasonographic measurements.

• The KSFM Journal of Fluid Machinery
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• v.10 no.4
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• pp.47-54
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• 2007

Simultaneous 3-D LDV measurements of the in-cylinder flows of three different engine setups were summarized for the quantification of the flow characteristics in each vertical or horizontal plane, and in entire cylinder volume. The ensemble averaged-velocity, tumble and swirl motions, and turbulent kinetic energy during the intake and compression strokes were examined from the measured velocity data (approximately 2,000 points for each engine setup). The better spatial resolution of the 3-D LDV allows measurements of the instantaneous flow structures, yielding more valuable information about the smaller flow structures and the cycle-to-cycle variation of these flow patterns. Tumble and swirl ratios, and turbulent kinetic energy were quantified as planar and volumetric quantities. The measurements and calculation results were animated for the visualization of the flow, and hence ease to analysis.