• 한국균학회지
• /
• 제12권4호
• /
• pp.175-181
• /
• 1984

Soil sieving을 통하여 4종(種)의 간석지식물(干潟地植物)들 (산조풀, 벌노랭이, 비쑥 및 띠등(等))과 관련되어 있는 Glomus속(屬)의 6종(種)과 Acaurospora 속(屬)의 1종(種)등 7종(種)의 vesicular-arbuscular mycorrhizal fungi를 분리(分離)하였다. Glomus속(屬)의 6종(種)은 G. albidum Walker & Rhodes, G. pulvinatum (Henn.)Trappe & Gerd., G. constrictum Trappe, G. sp. (4th type), G. caledonicum (Nicol. & Gerd.) Trappe & Gerd. (5th type) 및 G. sp. (6th type) 등이었으며, Acaulospora의 1종(種)은 A. scrobiculata 이었다. 7종(種)의 VA균(菌)가운데 G. mosseae (1st type), G. pulvinatum (2nd type) 및 G. sp.(4 type)의 3종(種)의 Glomus spp.가 가장 흔한 type들이었다. 산조풀과 비쑥의 근계토양(根系土壤)중에는 공(共)히 G. sp.(4th type)가, 벌노랑이와 띠의 근계토양(根系土壤)중에는 공(共)히 G. pulvinatum (2nd typc)이 가장 풍부(豊富)하게 존재(存在)한다는 사실(事實)을 알수 있었다. 본 연구(硏究)를 통(通)하여 토양(土壤)중 VA균종(菌種)의 다양성(多樣性)은 숙주식물에 달려있음을 확인(確認)할 수 있었다.

• 한국균학회지
• /
• 제18권4호
• /
• pp.191-197
• /
• 1990

접합내생균근은 세 식물군락(차풀, 솔새, 억새)의 토양에서 분리되었다. 분리된 포자는 형태학적으로 분류 동정한 결과 4속 8종이었다. 우리나라에서 새롭게 기술하는 종(種)은 2속 6종이다 ; Glomus aggregatium, Gl. ambisporum, Gl. geosporum, Gl. mosseae, Scutellospora coralloided, and Sc. heterogama.

• Journal of Microbiology
• /
• 제42권1호
• /
• pp.60-63
• /
• 2004

A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucrose-agar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.

• Journal of Microbiology and Biotechnology
• /
• 제2권1호
• /
• pp.66-71
• /
• 1992

The ecological distribution of endomycorrhizas in evergreen woody species native to the evergreen forest ecosystem of Tadohae-haesang National Park in southern Korea in February, 1989 was studied. The abundance and diversity of vesicular-arbuscular (VA) mycorrhizal fungi were also determined. The spore densities ranged from 14 to 326 per 100 g of soil. Most of the spores of mycorrhizal fungi collected from 25 soil samples belonged to the genera Glomus and Gigaspora. The frequency and number of spores in Camellia japonica varied with location. Spores belonging to the genus Gigaspora were not found in Camellia japonica in Yesongri evergreen forests adjacent to the sea. Glomus sp. was the major constituent of the spore assemblage at this site. The most abundant species in Camellia japonica in the Yesongri evergreen forests in Pogildo was Glomus borealis. In the soil of a mountain at Buwhangri, in the central location of the island at an elevation of 250 m, Gigaspora sp. was present and Glomus sp. was a major constituent of the spore assemblage. In the urban area of Haenam spore densities were much higher than in the Pogildo area. The most abundant species in Camellia japonica in the urban area of Haenam was Gigaspora sp..

• Journal of Microbiology and Biotechnology
• /
• 제15권1호
• /
• pp.147-154
• /
• 2005

A survey for vesicular-arbuscular mycorrhizae (VAM) status was undertaken in three different industrially polluted sites at Uyyakondan channel of Senthanneerpuram area in Trichy, India. The soils and the effluents were acidic, and contained higher Zn (621 to 711 ppm) than the other heavy metals, such as Cu, Pb, and Ni. Eighteen plant species were collected from the rhizosphere soils, and 13 species were positive for VAM colonization. Fifteen VAM fungal species were isolated from the plant species. The number of VAM fungal spores from the soils ranged from 45 to 640 per 100 g of soil. There was a significant correlation observed between the number of spores and percentage root colonization, as exemplified by Acalypha indica (45 and 20%, respectively) and Paspalum vaginatum (640 and 98%, respectively). Hostspecific and site-specific associations were observed in site 2; particular VAM species, Gigaspora gigantea and Glomus fasciculatum, were specific to particular host plants, Phyllanthus maderaspatensis and A. indica, respectively, even though Eclipta prostrata and Physalis minima were maximally associated with 8 VAM species. G. fasciculatum was found in 11 plant species and predominant VAM species. These results led us to conclude that VAM fungi are associated with a majority of the plants in the industrial polluted sites and support the plants to survive in the acidic soils, polluted with heavy metals of the industrial effluents.

• Journal of Ginseng Research
• /
• 제19권1호
• /
• pp.73-76
• /
• 1995

Newly sprouted American ginseng (Panax quinquefolium L.) seedlings were transplanted to forest pots with mycorrhizae-infested soil and grown in screenhouse for 2 years. Growth patterns, mortality rate and fresh root weight were investigated. Plants in VAM soil had lower mortality rales than control. In soils infested with two species of mycorrhizal fungi (Glomu deseyicola, frappe, Bloss and Merge and G. intraradices, Schenck and Smith), 28-35% of plants produced 3-prongs in the second season and significantly increased fresh root weight by 41 to 43%.

• 한국미생물·생명공학회지
• /
• 제21권2호
• /
• pp.95-100
• /
• 1993

Effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R455 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as as result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection.

• 한국토양비료학회지
• /
• 제27권1호
• /
• pp.54-63
• /
• 1994

딸기 모주(母株)로부터 얻은 포복경(匍匐莖)과 조직배양(組織培養)으로 얻은 딸기 묘(苗)에 가식시(假植時)와 순화처리(馴化處理) 전후(前後)의 시기에 딸기 근권토양(根圈土壤)에서 분리증식(分離增殖)한 VA균근균(菌根菌)을 접종(接種)시켜, 그 이후의 딸기 묘(苗) 생장반응(生長反應)을 조사(調査)하기 위하여 pot 실험(實驗)으로 비닐하우스에서 수행(遂行)하여 얻은 결과(結果)는 다음과 같다. VA균근균(菌根菌)이 감염(感染)된 딸기 묘(苗)들이 총엽수(總葉數), 엽장(葉長), 엽폭(葉幅) 및 엽병장(葉柄長)에서 고도(高度)의 유의적(有意的)인 증가(增加)를 보여 생육이 증진(增進)되었다. 포복경(匍匐莖)에서 얻은 딸기 묘(苗)의 건물중(建物重), 화수(花數) 및 영화수(穎花數)에서도 균근접종효과(菌根接種效果)가 인정되었으나, 개화시기(開花時期)에는 영향하지 않았다. VA균근균(菌根菌)을 접종하지 않은 조직배양(組織培養) 딸기 묘(苗)의 순화처리(馴化處理) 후 4주경부터 잎의 가장자리가 적갈색(赤褐色)(7.5R 4/8)으로 변하였고, 순화처리(馴化處理) 전후(前後)의 균근감염(菌根感染)된 딸기 묘(苗)에서 균근협생(菌根協生) 혜택이 확인되었는데 순화처리(馴化處理)와 동시에 접종처리하는 것이 생육(生育)과 생장량(生長量)이 많았다. 처리별 균근의존도(菌根依存度)는 포복경(匍匐莖)의 경우 162.7%, 순화처리(馴化處理) 전후(前後)의 접종처리에서 각각 116.4%, 106.0%였다. 균근감염(菌根感染)은 식물생장(植物生長)과 함께 증가(增加)되었고 실험(實驗) 종료시(終了時)의 감염율은 조직배양(組織培養) 묘(苗)와 포복경(匍匐莖)으로 번식된 묘(苗)에서 각각 47.5%와 56.4%였으며, 식물체(植物體)의 화학분석(化學分析) 결과(結果)는 인산, 칼리 및 석회가 균근(菌根) 접종처리(接種處理)에서 더 높은 함량(含量)을 보이고 마그네슘 함량(含量)은 그 반대였다. 본 실험결과(實驗結果)에서 확인(確認)되는 점은 딸기 묘(苗)에 VA균근균(菌根菌)을 접종하는 것이 초기생육(初期生育)을 증진(增進)시키기 때문에 가식기간(假植期間)을 포함한 딸기의 육묘단계(育苗段階)에서 이용(利用)이 기대된다.

• Journal of Microbiology and Biotechnology
• /
• 제14권4호
• /
• pp.885-890
• /
• 2004

A survey for vesicular-arbuscular mycorrhizae (VAM) occurrence was undertaken in three endangered vegetation sites in the area of Kudankulam atomic power station. Fifteen VAM fungal species were isolated from the root-zone soils of fourteen different plant species. There was a significant correlation observed between the number of spores and of percentage root colonization as exemplified by Phyllanthus niruri and Paspalum vaginatum (450, 95%; 60, 25%). Although VAM species are not known to be strictly site specific, the fact that Acaulospora elegans was observed only in site 1, Glomus pulvinatum in site 2 only, and Gl. intraradices in site 3 only, showed site-specificity in this study. To confirm the infection efficiency, two host plant species in the sites, P. niruri and Eclipta alba, were selected and inoculated in field with three selected VAM fungal spores. Gl. fasciculatum was found to be the most efficient VAM species in percentage root colonization, number of VAM spores, and dry matter content. When the nutrients in roots of P. niruri and E. alba were analyzed, there was higher uptake of K (4.2 and 3.4 times, respectively) and Ca (5.3 and 4.9 times, respectively), the analogues for $^{137}Cs$ and $^{90}Sr$, respectively. From the results, it might be concluded that VAM association helps the plants survive in a disturbed ecosystem and enhances uptake and cycling of radionuclides from the ecosystem.

• 한국토양비료학회지
• /
• 제40권6호
• /
• pp.476-481
• /
• 2007

친환경 재배지 토양과 관행토양간 토양화학성은 큰 차이를 보이지 않았으며, 토양 유효인산 함량은 친환경 재배지 토양이 관행 재배지 토양과 비교하여 다소 높은 수준이었다. 토양 관리방법에 따른 균근균 포자밀도는 친환경 재배지 토양이 건토 30 g 당 140여개의 포자밀도를 나타낸 반면 관행 재배지 토양은 60여개 내외의 포자밀도를 보였다. 각 토양 화학성과의 상관관계를 비교한 결과, pH, EC, OM, $Av.P_2O_5$, K Ca+Mg), CEC에 대한 관행재배지 토양의 균근균 포자밀도는 각각 $-0.48^*$, -0.05, $0.48^*$, -0.12, -0.13, 0.31의 상관계수를 나타냈다. 반면, 친환경 재배지 토양에 대한 균근균 포자밀도는 각각 $-0.68^*$, $0.69^*$, $0.96^{**}$, $0.75^*$, $0.63^*$, $0.92^{**}$의 상관계수를 보여 모든 무기성분이 유의성을 보였다. 뿌리의 균 조직은 주로 내부균사가 많았으며, 수지상체와 낭상체는 매우 낮은 수준이었다. 지역별로는 친환경 재배관리를 한 순천과 천안에서 다소 높은 수준의 감염정도를 나타냈다.