• Title/Summary/Keyword: Variance of Analysis

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Rapid Statistical Optimization of Cultural Conditions for Mass Production of Carboxymethylcellulase by a Newly Isolated Marine Bacterium, Bacillus velezensis A-68 from Rice Hulls (통계학적 방법을 사용한 해양미생물 Bacillus velezensis A-68균주의 섬유소 분해효소 생산 조건 최적화)

  • Kim, Bo-Kyung;Kim, Hye-Jin;Lee, Jin-Woo
    • Journal of Life Science
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    • v.23 no.6
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    • pp.757-769
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    • 2013
  • A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater, identified as Bacillus velezensis by analyses of 16S rDNA and partial sequences of the gyrA, and designated as B. velezensis A-68. The optimal conditions for production of CMCase by B. velezensis A-68 were established using response surface methodology (RSM). The optimal concentrations of rice hulls and yeast extract, and initial pH of the medium for cell growth were 60.2 g/l, 7.38 g/l, and 7.18, respectively, whereas those for production of CMCase were 50.0 g/l, 5.00 g/l, and 7.30. The analysis of variance (ANOVA) implied that the most significant factor for cell growth as well as production of CMCase was yeast extract. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ in the medium for cell growth were 7.50, 1.00, 0.10, and 0.80 g/l, respectively, which were the same as those for production of CMCase. The optimal temperatures for cell growth and production of CMCase were 30 and $35^{\circ}C$, respectively. The maximal production of CMCase under optimized conditions was 83.8 U/ml, which was 3.3 times higher than that before optimization. In this study, rice hulls, agro-byproduct, were developed as a substrate for production of CMCase and time for production of CMCase was reduced to 3 days using a newly isolated marine bacterium.

Measurement of competency through self study in basic nursing lab. practice focused on cleansing enema (기본간호학 실습에 있어 자가학습을 통한 능숙도 측정 - 배변관장을 중심으로 -)

  • Ko Il-Sun
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.6 no.3
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    • pp.532-543
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    • 1999
  • This study was conducted to provide the basic data necessary for the improvement of the teaching method for basic nursing practice as well as the effectiveness of the practice by examining the students' competency in cleansing enema after doing the self study instead of the traditional education. To examine the competency in cleansing enema after the self study, this study is an one group pretest-posttest design that subjects did the enema practice through the self study. The subjects were 89 sophomore students at Y University. College of Nursing. In basic nursing lab practice class, cleansing enema self study module was given to the students which was developed by the researcher based on the literature review and asked them to finish doing the pre study and checking the self study evaluation criteria after reading the goal, learning activities and theoretical guideline. After watching the video tape, students practiced the process in the module by themselves. For the competency in cleansing enema. repeated autonomous practices were done during the open lab other than the regular class. Whenever the practice was done, the frequency and time were measure and documented. When the student felt confident through repeated practices, the competency was evaluated by the researcher and two assistants based on the evaluation criteria. And the process was repeated till the student could perform all the items on evaluation criteria completely. The data were collected for 42 days from Oct. 15 to Nov. 26 in 1996. Collected data were analyzed by frequency, percentage, Pearson correlation coefficient and variance analysis. The results are summarized as follows : 1. 43.2% of the students were favorable to nursing and 63.6% like lecture, but 71.6% like practice. So they were more interested in practice than in lecture. 2. 62.3% of the students scored high in written test, 97.8% scored high in practice. So the practice score was better. 3. The frequency of repeated practice to pass the test ranged from 1 to 4 and the average is 2.2. 4. The average time needed in preparation and the performance was nearly the same regardless of the frequency. It took 5 to 38 minutes for those who passed the test after practicing once and the average was 16 minutes. 5 to 60 minutes were taken for those who practiced twice to pass the test and the average was 21 minutes. Those who passed the test after three practices needed 8 to 30 minutes and the average was 15 minutes, which was similar to the time that the students who passed the test for the first trial. Only one student passed the test after 4 practices and it took 10 minutes. 5. 64% of the students agreed that the context and the content of the module were appropriate for the self study and 68.2% were satisfied. And 71.9% said that the module helped them to practice the enema self study 6. Though only 42% of the students were satisfied with the video. 50.6% said that it was helpful for the self study. 7. 52.3% of the students were satisfied with the self study method, and 86.6% obtained self-confidence when performing the enema. 8. The lower the student's practice score was, the more practices were needed for them to pass the test(r=-.213, P<.05). As a result, for performing the enema practice competently, two or more practice opportunities were needed to be given. And it is possible to obtain the less complex nursing skills through the self study, when enough learning resources and assistance such as learning guidance or video tapes are provided. Based on this study. I want to suggest that. 1. There must be college policy that can support the new method instead of the traditional learning method for the students to attain the proficiency in basic nursing skills. 2. The assistant materials should be developed as soon as possible to promote the self study of basic nursing skills.

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A Study on the Relationships Between Demographic Characteristics of Entrepreneurial Candidates, their Developmental Level of Entrepreneurial Traits and Choice of Business Field (예비 창업자의 인구학적 특성과 창업성향 발달도 및 창업업종 선정에 관한 연구)

  • Choi, Joong Seog;Sung, Sang Hyeon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.10 no.5
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    • pp.1-13
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    • 2015
  • The purpose of this research is to do exploratory analysis on the relationships between demographic characteristics of entrepreneurial candidates and their developmental level of entrepreneurial traits as well as the choice of business field. We analyzed gender, age, and educational level of the entrepreneurial candidates and their developmental level of entrepreneurial traits such as achievement tendencies, degree of awareness, planning skills, sincerity, interpersonal relationship, and problem-solving skills. Industry fields were classified into 4 types: restaurants, retail, services, internet business. Descriptive statistical analyses, logistic regressions, and variance analyses were performed about the sample data gathered from 1,441 persons since the year 2002 to 2014. Males and highly educated above university preferred service industry than restaurant, retail, and internet business. Females preferred internet business, retail, and restaurants than service industry field. The young persons under 20 showed higher probability to choose internet business. The college level and high school graduates preferred retail and restaurants than service industry. The developmental level of entrepreneurial traits were higher in male and highly educated. By this study, we could find the divide between groups with high and low probabilities of failure: restaurants and retail field showed higher failure probability than service industry in the business fields of self-employed. Furthermore, we could identify that entrepreneurial traits also had been developed differently depending on the groups. It is necessary to refrain from entering into the fields with high failure probabilities as well as to provide supporting policies to build the systems and environment to reduce the cases to begin new business without special knowledges and know-hows. The competency development training should be provided in the early stage of start-ups considering their entrepreneurial traits and aptitudes. Not only the assessment of the entrepreneurial traits but also professional counseling from initial stage are necessary.

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Optimization of the Indole-3-Acetic Acid Production Medium of Pantoea agglomerans SRCM 119864 using Response Surface Methodology (반응표면분석법을 활용한 Pantoea agglomerans SRCM 119864의 Indole-3-acetic acid 생산 배지 최적화)

  • Ho Jin, Jeong;Gwangsu, Ha;Su Ji, Jeong;Myeong Seon, Ryu;JinWon, Kim;Do-Youn, Jeong;Hee-Jong, Yang
    • Journal of Life Science
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    • v.32 no.11
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    • pp.872-881
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    • 2022
  • In this study, we optimized the composition of the indole-3-acetic acid (IAA) production medium using response surface methodology on Pantoea agglomerans SRCM 119864 isolated from soil. IAA-producing P. aglomerans SRCM 119864 was identified by 16S rRNA gene sequencing. There are 11 intermediate components known to affect IAA production, hence the effect of each component on IAA production was investigated using a Plackett-Burman design (PBD). Based on the PBD, sucrose, tryptone, and sodium chloride were selected as the main factors that enhanced the IAA production at optimal L-tryptophan concentration. The predicted maximum IAA production (64.34 mg/l) was obtained for a concentration of sucrose of 13.38 g/l, of tryptone of 18.34 g/l, of sodium chloride of 9.71 g/l, and of L-tryptophan of 6.25 g/l using a the hybrid design experimental model. In the experiment, the nutrient broth medium supplemented with 0.1% L-tryptophan as the basal medium produced 45.24 mg/l of IAA, whereas the optimized medium produced 65.40 mg/l of IAA, resulting in a 44.56% increase in efficiency. It was confirmed that the IAA production of the designed optimal composition medium was very similar to the predicted IAA production. The statistical significance and suitability of the experimental model were verified through analysis of variance (ANOVA). Therefore, in this study, we determined the optimal growth medium concentration for the maximum production of IAA, which can contribute to sustainable agriculture and increase crop yield.

Monitoring of Atmospheric Aerosol using GMS-5 Satellite Remote Sensing Data (GMS-5 인공위성 원격탐사 자료를 이용한 대기 에어러솔 모니터링)

  • Lee, Kwon Ho;Kim, Jeong Eun;Kim, Young Jun;Suh, Aesuk;Ahn, Myung Hwan
    • Journal of the Korean Association of Geographic Information Studies
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    • v.5 no.2
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    • pp.1-15
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    • 2002
  • Atmospheric aerosols interact with sunlight and affect the global radiation balance that can cause climate change through direct and indirect radiative forcing. Because of the spatial and temporal uncertainty of aerosols in atmosphere, aerosol characteristics are not considered through GCMs (General Circulation Model). Therefor it is important physical and optical characteristics should be evaluated to assess climate change and radiative effect by atmospheric aerosols. In this study GMS-5 satellite data and surface measurement data were analyzed using a radiative transfer model for the Yellow Sand event of April 7~8, 2000 in order to investigate the atmospheric radiative effects of Yellow Sand aerosols, MODTRAN3 simulation results enable to inform the relation between satellite channel albedo and aerosol optical thickness(AOT). From this relation AOT was retreived from GMS-5 visible channel. The variance observations of satellite images enable remote sensing of the Yellow Sand particles. Back trajectory analysis was performed to track the air mass from the Gobi desert passing through Korean peninsular with high AOT value measured by ground based measurement. The comparison GMS-5 AOT to ground measured RSR aerosol optical depth(AOD) show that for Yellow Sand aerosols, the albedo measured over ocean surfaces can be used to obtain the aerosol optical thickness using appropriate aerosol model within an error of about 10%. In addition, LIDAR network measurements and backward trajectory model showed characteristics and appearance of Yellow Sand during Yellow Sand events. These data will be good supporting for monitoring of Yellow Sand aerosols.

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Physicochemical Properties and Tenderness of Hanwoo Loin and Round as affected by Raising Period and Marbling Score (사육기간과 등심의 근내지방도가 거세한우 등심 및 우둔의 이화학적 성상 및 연도에 미치는 영향)

  • Chin, Koo Bok;Go, Mi Young;Lee, Hong Chul;Chung, Seul Kee;Baik, Kyung Hoon;Choi, Chang Bon
    • Food Science of Animal Resources
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    • v.32 no.6
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    • pp.842-848
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    • 2012
  • This study was performed to investigate the physicochemical properties and tenderness of Hanwoo loin and round cuts, as affected by various raising periods and marbling scores. Five loin and round cuts with various marbling scores (5-9) from Hanwoo at various raising periods (27, 33, 30 mon) were collected. pH, Hunter color values (L, a, b), chemical composition, cooking loss, vacuum purge, and Warner-Bratzler shear values were evaluated according to two-way analysis of variance. No differences in pH values of Hanwoo loin were observed by raising period and marbling scores (p>0.05), but those of Hanwoo round at 27 mon was the lowest. Increased Hunter color values were observed with the increased marbling score and those at 27 and 30 mon were lower than 33 mon. Redness (a) and yellowness (b) of Hanwoo round cuts with the raising period of 27 mon were higher than those at 30 and 33 mon. The raising period of 27 mon showed higher water holding capacity than those of 30 and 33 mon, regardless of Hanwoo cut. Increased shear values (kgf) of Hanwoo round cuts were observed with increasing raising period (p<0.05). Increased marbling score decreased shear values in loin cuts, but no differences were observed in the round cuts. Based on these results, the parameters measured varied with meat cut, and raising period affected round pH, color, chemical composition, vacuum purge, and shear values. Marbling score affected color, chemical composition, water binding capacity, and shear values in loin cuts (p<0.05), but didn't affect any parameters in round cuts (p>0.05).

The Effects of Different Types of Treatment on EPOC and Anti-Oxidant Systems after Horseback Riding Exercise (승마기구운동 후 서로 다른 회복처치가 초과산소섭취량 및 항산화력에 미치는 영향)

  • Kim, Cheol-Woo;Baek, Yeong-Ho;Kwak, Yi-Sub
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1612-1618
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    • 2011
  • The present study was undertaken to analyze the effects of different types of treatment on excess post exercise oxygen consumption (EPOC), flexibility, free radical and antioxidants in women using a horseback riding therapeutic device. Subjects were trained in regular horseback riding exercises for 12 weeks (3 times/wk). The effects of this exercise were examined by means of a single session of horseback riding that lasted for 30 min. 21 women were recruited from a public health center and divided into 3 groups (passive recovery group, passive+massage recovery group, and dynamic recovery group). 3 types of recovery patterns were determined after a single trial of horseback riding exercise. Their flexibility were determined pre-and post-training by Paired T test, and ANOVA were used to analyze the data. The results were as follows: Among the 3 groups, the dynamic recovery group showed the highest levels of EPOC compared to the other groups, and also showed higher levels of anti-oxidants, as did the passive+massage recovery group compared to the passive recovery group. Moreover, horseback riding exercise greatly increased flexibility in the women. In conclusion, regular horseback riding training is recommended to enhance the flexibility of women and dynamic recovery is recommended to enhance EPOC and anti-oxidants after a single bout of exercise. Further study is needed in this area.

Sequential Sampling Plan for Aphis gossypii (Hemiptera: Aphididae) based on Its Intra-plant Distribution Patterns in Greenhouse Cucumber at Different Growth Stages (온실재배 오이의 생육단계별 목화진딧물의 주내 분포 특성에 기초한 축차표본조사법)

  • Chung, Bu-Keun;Song, Jeong-Heub;Lee, Heung-Su;Choi, Byeong-Ryul
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.401-407
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    • 2015
  • This study describes the development of a method for monitoring Aphis gossypii in greenhouse cucumber fields that was used during 2013 and 2014. The dispersion pattern of A. gossypii was determined by commonly used methods: Taylor's power law (TPL) and Iwao's patchiness regression (IPR). The sample unit was determined by linear regression analysis between mean density of sample unit versus whole plant. The optimum sample unit for different plant growth stages was two leaves (median and the lowest + 1 leaf) when the total number of leaves was less than nine, and three leaves (4th, 7th from canopy, and the lowest +1 leaf) when the total number of leaves was greater than nine. A. gossypii showed an aggregated distribution pattern, as the slopes of both TPL and IPR lines were greater than 1. TPL provided a better description of the mean-variance relationship than did IPR. The slopes and intercepts of TPL and IPR from leaf samples did not differ between the surveyed years. Fixed precision levels (D) for a sequential sampling plan were developed using Green's and Kuno's equations based on the number of aphid in a leaf sample. Green's method was more efficient than Kuno's to stop sampling. The number of samples needed to estimate the density of A. gossypii increased at higher D levels and lower mean densities. The cumulative number of aphids needed to stop sampling increased at higher D levels and with fewer plants sampled. Thus to estimate 10 aphids per leaf, 13 plants needed to be sampled, and the cumulative number of aphids to stop sampling was 131.

COMPARATIVE STUDY OF BEHAVIOR AND COGNITIVE FUNCTION BY ADMINISTRATION OF METHYLPHENIDATE AND IMIPRAMINE IN ATTENTION DEFICIT-HYPERACTIVITY DISORDER (Methylphenidate와 Imipramine투여에 따른 주의력 결핍${\cdot}$과잉운동장애 환아의 행동 및 인지기능 변화에 대한 연구)

  • Ahn, D.H;Hong, K.E;Oh, K.J;Shin, M.S;Yoo, B.C;Chung, K.M
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.3 no.1
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    • pp.26-45
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    • 1992
  • This study presents the behavioral and cognitive changes by administration of methylphenidate(MPH) and imipramine(IMI) for the treatment of attention-deficit hyperactivity disorder(ADHD) in $5_{1/2}{\sim}12$ years old children referred to child psychiatric clinics. Behavioral changes are assessed with parent's and teacher's ratings. Drug effects on attention. short-term memory, and impulsivity are evaluated with psychological tests in laboratory. The changes were assessed twice in a 8-week periods. The data were analyzed seperately for 15 subjects each drug using repeated measured analysis of variance(ANOVA). The findings indicates that behavioral and cognitive impairments are improved by both drugs, but impulsivity is not. And MPH is superior to IMI on the improvement of attentional problem ; especially the findings indicates important differences between simple task and complex. perceptual-search task. These data confirm the effectiveness of MPH for treatment of ADHD, also raise questions regarding assessment method of attention and impulsivity as fell as importance of impulsivity in ADHD.

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Modeling and mapping fuel moisture content using equilibrium moisture content computed from weather data of the automatic mountain meteorology observation system (AMOS) (산악기상자료와 목재평형함수율에 기반한 산림연료습도 추정식 개발)

  • Lee, HoonTaek;WON, Myoung-Soo;YOON, Suk-Hee;JANG, Keun-Chang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.3
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    • pp.21-36
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    • 2019
  • Dead fuel moisture content is a key variable in fire danger rating as it affects fire ignition and behavior. This study evaluates simple regression models estimating the moisture content of standardized 10-h fuel stick (10-h FMC) at three sites with different characteristics(urban and outside/inside the forest). Equilibrium moisture content (EMC) was used as an independent variable, and in-situ measured 10-h FMC was used as a dependent variable and validation data. 10-h FMC spatial distribution maps were created for dates with the most frequent fire occurrence during 2013-2018. Also, 10-h FMC values of the dates were analyzed to investigate under which 10-h FMC condition forest fire is likely to occur. As the results, fitted equations could explain considerable part of the variance in 10-h FMC (62~78%). Compared to the validation data, the models performed well with R2 ranged from 0.53 to 0.68, root mean squared error (RMSE) ranged from 2.52% to 3.43%, and bias ranged from -0.41% to 1.10%. When the 10-h FMC model fitted for one site was applied to the other sites, $R^2$ was maintained as the same while RMSE and bias increased up to 5.13% and 3.68%, respectively. The major deficiency of the 10-h FMC model was that it poorly caught the difference in the drying process after rainfall between 10-h FMC and EMC. From the analysis of 10-h FMC during the dates fire occurred, more than 70% of the fires occurred under a 10-h FMC condition of less than 10.5%. Overall, the present study suggested a simple model estimating 10-h FMC with acceptable performance. Applying the 10-h FMC model to the automatic mountain weather observation system was successfully tested to produce a national-scale 10-h FMC spatial distribution map. This data will be fundamental information for forest fire research, and will support the policy maker.