• 자원ㆍ환경경제연구
• /
• 제12권1호
• /
• pp.49-74
• /
• 2003

This study used a slightly modified version of contingent valuation questionnaire designed and developed by Krupnick et al.(2000) to estimate the value of mortality risk reduction for environmental policy. Because that environmental policy, especially air pollution policy with some latency main effect on mortality risk reduction of old people with some latency period, respondents were asked about their current WTP for 5/1,000 mortality risk reduction being to take place beginning at 70 over the course of ten years. The annual mean WTP is 233,370 won (standard error 32,570 won) in spike/continuous/interval data model. The implied VSL is 466 million won. The 95% confidence interval is 339 million won~594 million won.

• Wind and Structures
• /
• 제26권4호
• /
• pp.253-266
• /
• 2018

In this paper, the aerodynamic performance of two new cable surfaces with concave fillets (strakes) is examined and compared to plain, dimpled and helically filleted surfaces. To this end, an extensive wind-tunnel campaign was undertaken. Different samples with different concave fillet heights for both new surfaces were tested and compared to traditional surfaces in terms of aerodynamic forces (i.e. drag and lift reduction) and rain-rivulet suppression. Furthermore, flow visualization tests were performed to investigate the flow separation mechanism induced by the presence of the concave fillet and its relation to the aerodynamic forces. Both new cable surfaces outperformed the traditional surfaces in terms of rain-rivulet suppression thanks to the ability of the concave shape of the fillet to act as a ramp for the incoming rain-rivulet. Furthermore, both new surfaces with the lowest tested fillet height were found to have drag coefficients in the supercritical Reynolds range that compare favorably to existing cable surfaces, with an early suppression of vortex shedding.

• 대한기계학회논문집
• /
• 제18권9호
• /
• pp.2431-2442
• /
• 1994

In this paper the efficient space marching Viscous Shock Layer and Parabolized Navier-Stokes method have been applied to study the complex 3-D hypersonic equilibrium chemically reacting flowfilelds over sphere-cone($10^{\circ}$) vehicle at low angles of attack($0^{\circ}~5^{\circ}), Mach 20, and an altitude of 35km. The current bluntbody/afterbody space marching numerical method predicts the complex flowfields accurately and efficiently even on a small computer. The shock thickness from equilibrium air model is thinner than that from the perfect gas model. The windside wall heat-transfer rate, pressure and skin friction force were increased significantly when compared with those of leeside. The CA, CN, CM were increased almost linearly with the angle of attack in this region. The wall pressure, heat transfer, skin friction and axial force coeffient from equilibrium model were much higher than those from perfect gas model. The center of pressure moved forward with the increase of angle of attack.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1997년도 추계학술대회 논문집 학회본부
• /
• pp.471-475
• /
• 1997

PWM-VSI based ac-drives have high nonlinearity due to dead-time in the inverter and the voltage drop across the switching devices. In this paper, we introduced a new nonlinear model of PWM-VSl including parastic capacitor and also showed validity of the model by circuit simulations and experiments. Furthermore, we proposed an on-line identification algorithm for the uncertain model parameters.

• 한국전산구조공학회:학술대회논문집
• /
• 한국전산구조공학회 2007년도 정기 학술대회 논문집
• /
• pp.677-682
• /
• 2007

Nonlinear analysis of mixed structures is carried out by utilizing contact elements of a general finite element analysis computer program(ABAQUS). The present analysis focuses on the enhancing behaviors of mixed structure's connection type. Main 2 issues are related with discontinuity which reduce the stiffness of structure and proposing enhanced connection type. To validate the present study approaching 2 way, analytic one and experimental test.

• 한국수정란이식학회지
• /
• 제18권1호
• /
• pp.43-50
• /
• 2003

정자의 동결보존을 위한 새로운 기술개발 목적은 동결과정에서 최소한의 손상으로, 응해 후 최대한 높은 활력도의 정자를 얻는 것이다 정자가 난자와 수정하기 위해서는 적당한 생존성과 운동성을 유지해야 하는데, 가장 일반적인 방법으로는 정자의 진진 운동성과 첨체의 정상 여부 및 형태 검사방법 등이 있다 본 연구는 사람 정액을 동결보존 할 때 semi-programmable freezer를 이용한 완만동결 방법과, 액체질소의 vapor를 이용한 급속동결 방법이 응해 후 정자의 운동양상과 생존율 및 형태에 미치는 영향을 알아보기 위해 실시하였다. 동결-응해 후 정자의 MOT, VCL, VSL, VAP는 각각 급속 동결방법에서 47.40$\pm$20.06%, 38.12$\pm$15.58 $\mu$m/s, 28.19$\pm$14.10 $\mu$m/s, 33.64$\pm$15.15 $\mu$m/s로 완만 동결방법인 43.39$\pm$18.79%, 33.91$\pm$13.50 $\mu$m/s, 19.98$\pm$10.88 $\mu$m/s, 24.60$\pm$11.72 $\mu$m/s보다 유의적으로 높았으나(p<0.05), LIN은 완만동결 방법이 34.64$\pm$11.36으로 급속동결 방법인 28.83$\pm$10.35보다 더 좋은 성적을 나타내었다(p<0.05). 고 활력정자를 나타내는 HYP 역시 급속동결 방법에서 2.77$\pm$2.71%로 완만동결 방법의 1.33$\pm$1.57%보다 유의적으로 높게 나타났다(p<0.05). 그러나 정자의 운동양태를 나타내는 MAD, WOB, DNC, DNM에 있어서는 완만동결 방법이 급속동결 방법에 비해 조금 더 좋은 성적을 보였으나 유의적인 차이는 없었다. 동결-응해 후 정자의 생존율 및 정상형태의 정자는 완만동결 방법이 각각 62$\pm$2.1%, 44$\pm$8.3%, 급속동결 방법은 60$\pm$2.2%, 46$\pm$7.7%로 유의적인 차이가 없이 비슷한 결과를 보였다. 따라서 사람 정액의 동결 보존 시 많은 시간과 고가의 장비가 필요한 완만동결 방법보다는 짧을시간동안 액체 질소만으로 간단히 시행할 수 있는 급속동결 방법이 더 효과적이라고 사료된다.

• 한국수정란이식학회지
• /
• 제31권3호
• /
• pp.293-297
• /
• 2016

For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

• 한국수정란이식학회지
• /
• 제31권3호
• /
• pp.281-285
• /
• 2016

Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as a candidate gene to investigate Duroc boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT, $82.27{\pm}5.58$), Curvilinear velocity(VCL, $68.37{\pm}14.58$), Straight-line velocity(VSL, $29.06{\pm}6.58$), the ratio between VSL and VCL(LIN, $47.36{\pm}8.42$), Amplitude of Lateral Head displacement(ALH, $2.88{\pm}0.70$)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p<0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

• 한국식품영양과학회지
• /
• 제33권2호
• /
• pp.451-454
• /
• 2004

본 실험의 목적은 Saccharomyces cereuisiea를 단백분해효소로 처리하여 얻은 가수분해물 SCP-20의 첨가가 마우스의 생식 기능에 미치는 영향을 평가하기 위하여 실시하였다. 개시시 체중 32∼33 g의 7주령 ICR 웅성 마우스 40수를 공시하여 SCP-20의 급여가 정소무게 및 정소상체 미부 정자의 운동특성 미치는 영향을 평가하였다. 실험 설계는 분쇄한 가루형태의 일반 마우스 식이를 급여 한 대조군(CON)과 대조군 식이에 효모가수분해물 SCP-20을 1.5% 첨가한 처리군(TRT)으로 하였다. 정소무게에 있어서는 대조군과 비교하여 처리군의 정소무게가 유의적으로 더 높게 평가되었다. (p<0.05). 정자의 직선운동속도(VSL)는 대조군과 비교하여 처리군에서 유의적으로 높았다(p<0.05). 평균경로속도(YAP)와 곡선경로선형도(LIN)는 대조군과 비교하여 처리군이 더 높았다(p<0.05). 또한, 웅성 마우스에게 SCP-20을 급여하면 정소상체 미부 정자의 운동속도가 증가하는 것으로 나타났다(p<0.05). 결론적으로, 마우스에게 SCP-20를 급여하면 정자의 운동속도가 증가하는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권10호
• /
• pp.1417-1425
• /
• 2014

The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.