Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Association study analysis of phospholipase C zeta gene polymorphism forsperm motility and kinematic characteristics in liquid semen of Boar

Phospholipase C zeta 유전자의 유전적다형성과 돼지 액상정액의 운동학적 특성과의 연관성 분석

  • Jeong, Yong-dae (National Institute of Animal Science, Rural Development Adminstration) ;
  • Jeong, Jin-Young (National Institute of Animal Science, Rural Development Adminstration) ;
  • Sa, Soo-Jin (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Ki-Hyun (National Institute of Animal Science, Rural Development Adminstration) ;
  • Cho, Eun-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Yu, Dong-Jo (National Institute of Animal Science, Rural Development Adminstration) ;
  • Park, Sungk-won (Department of Food Science & Technology, Sejong University) ;
  • Jang, Hyun-Jun (College of Pharmacy, Dankook University) ;
  • Woo, Jae-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Choi, Jung-Woo (College of Animal Life Science, Kangwon National University)
  • 정용대 (농촌진흥청 국립축산과학원) ;
  • 정진영 (농촌진흥청 국립축산과학원) ;
  • 사수진 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 조은석 (농촌진흥청 국립축산과학원) ;
  • 유동조 (농촌진흥청 국립축산과학원) ;
  • 박성권 (세종대학교 생명과학대학 식품생명공학과) ;
  • 장현준 (단국대학교 약학대학) ;
  • 우제석 (농촌진흥청 국립축산과학원) ;
  • 최정우 (강원대학교 동물생명과학대학)
  • Received : 2016.09.09
  • Accepted : 2016.09.29
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

Keywords

References

  1. Cassady JP, Johnson R, Pomp D, Rohrer G, Van Vleck LD, Spiegel E, Gilson K. 2001. Identification of quantitative trait loci affecting reproduction in pigs. Journal of Animal Science 79:623-633. https://doi.org/10.2527/2001.793623x
  2. Chen X, Zhu H, Hu C, Hao H, Zhang J, Li K, Zhao X, Qin T, Zhao K, Zhu H. 2014. Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC-MS/MS. Reproduction 147:321-330. https://doi.org/10.1530/REP-13-0313
  3. Diniz D, Lopes M, Broekhuijse M, Lopes P, Harlizius B, Guimaraes S, Duijvesteijn N, Knol E, Silva F. 2014. A genome-wide association study reveals a novel candidate gene for sperm motility in pigs. Animal Reproduction Science 151:201-207. https://doi.org/10.1016/j.anireprosci.2014.10.014
  4. Frungieri MB, Gonzalez-Calvar SI, Parborell F, Albrecht M, Mayerhofer A, Calandra RS. 2006. Cyclooxygenase-2 and prostaglandin F2${\alpha}$ in Syrian hamster Leydig cells: inhibitory role on luteinizing hormone/human chorionic gonadotropinstimulated testosterone production. Endocrinology 147:4476-4485. https://doi.org/10.1210/en.2006-0090
  5. Gunawan A, Cinar M, Uddin M, Kaewmala K, Tesfaye D, Phatsara C, Tholen E, Looft C, Schellander K. 2012. Investigation on association and expression of ESR2 as a candidate gene for boar sperm quality and fertility. Reproduction in Domestic Animals 47:782-790. https://doi.org/10.1111/j.1439-0531.2011.01968.x
  6. Heytens E, Parrington J, Coward K, Young C, Lambrecht S, Yoon S-Y, Fissore R, Hamer R, Deane C, Ruas M. 2009. Reduced amounts and abnormal forms of phospholipase C zeta ($PLC{\zeta}$) in spermatozoa from infertile men. Human Reproduction 24:2417-2428. https://doi.org/10.1093/humrep/dep207
  7. Kaewmala K, Uddin M, Cinar M, Grosse Brinkhaus C, Jonas E, Tesfaye D, Phatsara C, Tholen E, Looft C, Schellander K. 2011. Investigation into Association and Expression of PLCz and COX 2 as Candidate Genes for Boar Sperm Quality and Fertility. Reproduction in Domestic Animals 47:213-223.
  8. Kurokawa M, Sato K-i, Wu H, He C, Malcuit C, Black SJ, Fukami K, Fissore RA. 2005. Functional, biochemical, and chromatographic characterization of the complete [Ca 2+] i oscillation-inducing activity of porcine sperm. Developmental Biology 285:376-392. https://doi.org/10.1016/j.ydbio.2005.06.029
  9. Le H, Nott A, Moore M. 2003. How introns influence and enhance eukaryotic gene expression. Trends Biochemical Sciences 28: 215-220. https://doi.org/10.1016/S0968-0004(03)00052-5
  10. Maes D, Nauwynck H, Rijsselaere T, Mateusen B, Vyt P, de Kruif A, Van Soom A. 2008. Diseases in swine transmitted by artificial insemination: an overview. Theriogenology 70:1337-1345. https://doi.org/10.1016/j.theriogenology.2008.06.018
  11. Ren D, Ren J, Xing Y, Guo Y, Wu Y, Yang G, Mao H, Huang L-S. 2009. A genome scan for quantitative trait loci affecting male reproductive traits in a White Duroc$\times$ Chinese Erhualian resource population. Journal of Animal Science 87:17-23. https://doi.org/10.2527/jas.2008-0923
  12. Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, Lai FA. 2002. $PLC{\zeta}$: a sperm-specific trigger of Ca2+ oscillations in eggs and embryo development. Development 129:3533-3544.
  13. Sirianni R, Chimento A, De Luca A, Zolea F, Carpino A, Rago V, Maggiolini M, Ando S, Pezzi V. 2009. Inhibition of cyclooxygenase-2 down-regulates aromatase activity and decreases proliferation of Leydig tumor cells. Journal of Biological Chemistry 284:28905-28916. https://doi.org/10.1074/jbc.M109.041020
  14. Walter F. 2003. Medical Physiology: A Cellular and Molecular Approach. Elsevier, Saunders 108.
  15. Yamaguchi K, Ishikawa T, Kondo Y, Fujisawa M. 2008. Cisplatin regulates Sertoli cell expression of transferrin and interleukins. Molecular and Cellular Endocrinology 283:68-75. https://doi.org/10.1016/j.mce.2007.11.021
  16. Zeng C, He L, Peng W, Ding L, Tang K, Fang D, Zhang Y. 2014. Selection of optimal reference genes for quantitative RT-PCR studies of boar spermatozoa cryopreservation. Cryobiology 68:113-121. https://doi.org/10.1016/j.cryobiol.2014.01.004