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Urushiol V Suppresses Cell Proliferation and Enhances Antitumor Activity of 5-FU in Human Colon Cancer Cells by Downregulating FoxM1

  • Jeong, Ji Hye;Ryu, Jae-Ha
    • Biomolecules & Therapeutics
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    • v.30 no.3
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    • pp.257-264
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    • 2022
  • Colorectal cancer (CRC) is one of the most common malignant tumor. 5-FU is commonly used for the treatment of CRC. However, the development of drug resistance in tumor chemotherapy can seriously reduce therapeutic efficacy of 5-FU. Recent data show that FoxM1 is associated with 5-FU resistance in CRC. FoxM1 plays a critical role in the carcinogenesis and drug resistance of several malignancies. It has been reported that urushiol V isolated from the cortex of Rhus verniciflua Stokes is cytotoxic to several types of cancer cells. However, the underlying molecular mechanisms for its antitumor activity and its potential to attenuate the chemotherapeutic resistance in CRC cells remain unknown. Here, we found that urushiol V could inhibit the cell proliferation and induced S-phase arrest of SW480 colon cancer cells. It inhibited protein expression level of FoxM1 through activation of AMPK. We also investigated the combined effect of urushiol V and 5-FU. The combination treatment reduced FoxM1 expression and consequently reduced cell growth and colony formation in 5-FU resistant colon cancer cells (SW480/5-FUR). Taken together, these result suggest that urushiol V from Rhus verniciflua Stokes can suppress cell proliferation by inhibiting FoxM1 and enhance the antitumor capacity of 5-FU. Therefore, urushiol V may be a potential bioactive compound for CRC therapy.

Isolation and Identification of Vibrio vulnificus and Vibrio parahaemolyticus from Coast of Pusan and Daechon (부산과 대천 해안에서 Vibrio vulnificus와 Vibrio parahaemolyticus의 분리 및 동정)

  • Ju, Jin-Woo;Park, Min-Jung;Heo, Moon-Soo;Jung, Cho-Rok
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.4
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    • pp.309-316
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    • 2000
  • This study was focused on the isolation of pathogenic Vibrio species, V. vulnificus and V. parahaemolyticus from marine environment from May to July of 1999. Isolation sites were coast near by Pusan and Daechon. The results obtained were as follows: 1. Seventy strains of V. parahaemolyticus and 19 strains of V. vulnificus were isolated from a total of 120 specimens. 2. Nineteen strains of V. vulnificus did not fermented arabinose and salicin but fermented lactose and cellobiose. All of V. parahaemolyticus isolates did not fermented lactose and cellobiose. 47 strains of V. parahaemolyticus fermented arabinose but 53 strains did not fermented salicin. 3. V. vulnificus and V. parahaemolyticus isolates showed three different API index numbers with 5046105 and 4346107 dominant. 4. V. vulnificus did not grow on 0% and 8% NaCl containing medium. V. parahaemolyticus grew on 8% NaCl containing medium. 5. V. vulnificus isolates and V. parahaemolyticus revealed different outer membrane protein profiles on SDS-PAGE.

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The Performance of Li/V6O13 Lithium Polymer Battery (Li/V6O13 리튬 폴리머 전지의 성능)

  • Kim, Hyung-Sun;Cho, Byung-Won;Yun, Kyung-Suk;Chun, Hai-Soo
    • Applied Chemistry for Engineering
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    • v.7 no.2
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    • pp.362-370
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    • 1996
  • The performance of The performance of $Li/V_6O_{13}$ cell and the electrochemical properties of polymer electrolyte based on poly(acrylonitrile)[PAN] was investigated. The ionic conductivity of polymer electrolyte showed $2.3{\times}10^{-3}S/cm$ and the compatibility with lithium electrode was excellent. Also, it showed the electrochemical stability up to 4.3V(vs. $Li^+/Li$). The cell reaction of $Li/V_6O_{13}$ was dominated by the interfacial resistance between $V_6O_{13}$ electrode and polymer electrolyte. The diffusion coefficient of lithium ion within $V_6O_{13}$ was $2.7{\times}10^{-9}{\sim}4.2{\times}10^{-8}cm^2/sec$. The utilization of $V_6O_{13}$ active material was 95% at C/8($50{\mu}A/cm^2$) and 82% at C/4($100{\mu}A/cm^2$), respectively.

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Adsorptive Catalytic Wave of Chromium-Cupferron Complex (크롬-쿠페론 착물의 흡착 촉매파)

  • Kwon, Young-Soon;Seo, Soh-Jin;Lee, Sang-Mi
    • Analytical Science and Technology
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    • v.15 no.3
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    • pp.214-220
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    • 2002
  • The interfacial accumulation of the chromium-cupferron complex and the catalytic wave of its redox process is characterized by cyclic voltammetry. One cathodic peak is observed in the forward scan at -1.45 V. Scanning in the reverse direction produces a inverted peak at -1.39 V, which is indicative of a catalytic process. The optimal conditions of inverted peak were found to be 1 mM borate buffer solution(pH 9.48) containing $1{\times}10^{-4}M$ cupferron, holding potential of -1.8 V and scan rate of 20 mV/s. Using main peak, a preconcentration time of 1 min results in a detection limit of $3.2{\times}10^{-10}M$.

Visible photochromic energy shift of $WO_{3}$/CdS thin films fabricated by thermal evaporation method (진공증착 법으로 제작한 $WO_{3}$/CdS 박막의 가시광 광 변색의 에너지 전환)

  • Kim, Keun-Mook;Kim, Myung-Wook
    • Journal of the Semiconductor & Display Technology
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    • v.4 no.1 s.10
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    • pp.29-34
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    • 2005
  • Tungsten oxide($WO_{3}$) is suitable to materials for photochromic window in the visible region. The resistivities of CdS, $WO_{3}$, and $WO_{3}$/CdS films prepared by thermal evaporation method were $4.61\times 10\^{3}$, $7.59\times10^{3}$, and $6.29\times10^{3}$ $\omega$ cm. And x-ray diffraction patterns of CdS, $WO_{3}$/CdS films showed a preferred orientation of hexagonal(002), and the monoclinic(020) structure, respectively. The optical transmission were measured that the cut-on wavelength were 510nm, 380nm for CdS and $WO_{3}$ films respectively, and the transmission spectrum of $WO_{3}$/CdS was shifted into the visible region. Photoluminescence(PL) spectra showed the two peaks at 2.8 eV and 3.2 eV for the as-grown sample($WO_{3}$/CdS ($500{\AA}$), but the other sample($WO_{3}$/CdS ($1000{\AA}$)) had a peak energy value of 2.8 eV. The photochromism of $WO_{3}$/CdS films showed that the excitation of electron-hole pairs and subsequent coloration is shifted into visible-light range. And the spectral behavior of coloration turned out to be proportional to the excited electron-hole pairs creation rate of CdS film. This result is interpreted in terms of charge carrier injection from the CdS-layer into the $WO_{3}$ films. We found a value of about 2.8 eV of $WO_{3}$/CdS film which is somewhat higher than peak energy of 2.54 eV using CBD prepared by Bechinger et. al.

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Features of Epitaxial Garnet Films for an Atomic Traps Technique

  • Berzhansky, V.N.;Vishnevskii, V.G.;Nedviga, A.S.;Nesteruk, A.G.
    • Journal of Magnetics
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    • v.14 no.3
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    • pp.108-113
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    • 2009
  • High-coercive garnet films have certain parameters suitable for creating reconfigurable magnetic atomic chips with visible geometry. However, the inner stresses and morphological properties, namely, networks of dislocations, layering and surface relief, and dependence of coercivity on thickness must be taken into account. Select features of films important for atomic trap creation have been studied experimentally and the supposed traps concept provided.

A 1.25Gb/s Burst-mode Optical Transmitter with Digitally Controlled APC (디지털 제어 방식의 APC 기능을 갖는 1.25Gb/s 버스트-모드 광 송신기)

  • Ki, Hyeon-Cheol
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.12
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    • pp.25-30
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    • 2007
  • In this paper, we proposed a new burst-mode optical transmitter structure which is suitable for high data rate operation such as Gb/s operation. With this structure we made a 1.25Gb/s burst-mode optical transmitter including a digitally controlled APC circuit for EPON systems using commercial 0.8m BiCMOS technology. It well functioned at 1.25Gb/s and showed good eye patterns with 53.3ps jitter, 191ps rise time and 258ps fall time. To characterize the APC function we measured optical output power as increasing external voltage VREF. The optical power is linearlyproportional to VREF at the rate of 0.293mW/V.

Importance of Iron in the Toxicity of Vibrio vulnificus (Vibrio vulnificus의 독성에 있어서 Iron의 중요성)

  • 이봉헌;박흥재
    • Journal of Environmental Science International
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    • v.7 no.4
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    • pp.501-504
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    • 1998
  • The role of iron as a possible pathogenic factor in the Infection of V. vulnificus was examined in thins paper The effects of iron and $CCl_4$ on the growth of V. vulnificus in human and rabbit sera were also done. Injection of iron to mice resulted in a lowering of the 50% lethal dose and in a reduction in the time of death postinfection. Serum iron levels were also elevated by damaged livers with infections of $CCl_4$- The inoculum size required to kill these mice was directly correlated with serum iron Irvels. Iron appeared to be the limiting factor In the ability of thins organism to survive or grow in mammalian sera. These results, both in vitro and In vivo, provided strong evidence that iron may play a major role In the pathogenesis of V. vulnificus.

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A Study on the Water Relation Characteristics of the Soybean Shoots through the Pressure Chamber Technique (Pressure Chamber Technique에 의한 대두경엽의 수분특성 연구)

  • E-Hun Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.128-132
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    • 1983
  • The water relations parameters such as original osmotic pressure $(\Pi_0)$, osmotic pressure $(\Pi_p)$, at the incipient plasmolysis, the volume of osmotic water $(V_o)$ at the full turgor, the relative water content $(RWC^*)$ at the incipient plasmolysis, the volume of osmotic water$(V_p)$ at the incipient plasmolysis, the volume of symplasmic and apoplastic water$(w_s)$ at the maximum turgor, $V_pV_o, \; V_oW_s, \; W_sD_w, \; V_oF_w$ and the elastic modulus were measured through the pressure chamber (DIK-PC-40 Model) technique with the Williams and Geumgangdaerip soybean cultivar shoots. The original osmotic pressure$(\P $i_{0}$ )$, of the both cultivars shoots were appeared insignificant differences as 8.1 bar and 7.8 bar respectively. The osmotic pressure($\pi$$_{p}$ ) at the incipient plasmolysis was 9.0 bar in Williams and 10.4 bar in Geumgangdaerip. The relative water content(RW $C^{*}$) at the incipient plasmolysis was 86.0 per cent in Geumgangdaerip and 92.6 per cent in Williams. The ratio of volume of osmotic water( $V_{p}$ ) at the incipient plasmolysis to total symplasmic water$(V_o)$ , i.e., $V_p/V_o$ was 83.4 per cent in Geumgangdaerip and 90.4 per cent in Williams. The elastic modulus of Geumgangdaerip shoots showed higher value than Williams$(8.5 $\times$ 10^2bar)$ as $1.6 \times10^5$ bar.r.

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Verfremdung Effekt(V-Effekt) in Korean, Chinese, and Japanese Traditional Play Costumes - Focusing on masked drama, Beijing opera, and Kabuki - (한.중.일 전통극 복식의 소외효과(V-Effekt) 연구)

  • Lee, Mi-Sook;Yang, You-Mee
    • Journal of the Korean Society of Costume
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    • v.60 no.1
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    • pp.14-27
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    • 2010
  • The purpose of this study is to analyze how the aesthetic characteristics of Brecht's V-Effekt is visually expressed of the costume in the Traditional Play of Korea, China and Japan. The method and the contents of the study were to refer to the antecedent studies and the related documents to peruse the characteristics of the traditional plays of the East and West, the origin and the concept of Brecht's Verfremdung and considered the relation of Brecht's V-Effekt and the Oriental plays, and then the researcher derived the aesthetic properties of Brecht's V-Effekt. This study analyzed how those qualities of the aesthetic characteristics on the V-Effekt are manifested on the stage costumes of Korean mask, Beijing opera and Kabuki. The aesthetic qualities of Brecht's V-Effekt are classified into symbolism, grotesque disposition, character of sing and dance, comicality. The symbolism in the traditional play costumes of the three nations is shown in the colors of the masks in Korean and the tone of the colors on the costumes and the make-up in Beijing opera and Kabuki. While the properties of bizarrerie and deformation coexist in masque and Beijing opera costumes in terms of grotesque disposition, Kabuki strongly displays bizarre grotesquerie. The character of sing and dance is visually expressed through the transformation of sleeves on the three nation's traditional play costumes; Masque on the Hansam and Chengsam, Beijing opera on the Water sleeves and Kabuki on Hurisode. The comic aspection is expressed in a humorous and comical way through the distortion and transformation of forms in Masque and Beijing opera but it cannot be seen in Kabuki costume. The study as above will form the aesthetic properties of the Oriental traditional play costumes and also it will contribute to establishing the identity of Korean mask costumes.