• Title/Summary/Keyword: V$_{}$ 50/

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Glycerol Addition for the Hyper-production and Stabilization of a Novel Carbohydrolase by Lipomyces starkeyi (전분을 이용한 탄수화물 분해효소의 고 생산과 효소 안정성 증가를 위안 글리세롤 첨가)

  • 이선옥;이진화;박준성;서은성;김창용;조동련;김도원;김도만
    • KSBB Journal
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    • v.17 no.6
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    • pp.586-589
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    • 2002
  • Lipomyces starkeyi KSM 22 produces dextranase and amylase (DXAMase). The addition of 0.02% (w/v) 2-deoxy-D-glucose or 0.5% (w/v) glycerol into a 1% (w/v) starch medium increased the final activity of DXAMase produced 2.5 fold or 2.4 fold, respectively, compared to that of a 1% (w/v) starch medium. This activity was similar to that produced with 1% (w/v) dextran. The stability of purified OXAMase at 40$\^{C}$ for 3 weeks was tested using the various enzyme stabilizers. With the addition of 25% (v/v) glycerol, 90.9% of initial activity was left after 3 weeks. For practical use, the addition of 1% (v/v) glycerol with 50 mM of CaCl$_2$ or KH$_2$PO$_4$was adequate and maintained 73.4% of the initial activity under the test conditions used.

Change of Volume of Isoflow in Pneumoconiosis Patients with Small Opacity (소음영 진폐증 환자에서의 등기류용량(Volume of Isoflow)의 변화)

  • Oh, Sang-Yong;Kim, Jee-Won;Jung, Chang-Young;Kim, Kyung-Ah;Yun, Im-Goung
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.5
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    • pp.540-547
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    • 1993
  • Backgrounds: The measurement of volume of isoflow has been considered as a sensitive test for detecting small airway diseases showing normal pulmonary function in a routine pulmonary function test. To evaluate the functions of small airway among dust exposed workers, the changes of volume of isoflow were measured and its applicability of managing early stage pneumoconiosis patients was studied. Method: The subjects were 67 male, pneumoconiosis with small opacity and FEV1>80%, FEV1/FVC>75% in spirometry and the controls were 20 male, no dust-exposed office workers. The maximal epiratory volume curves after inhalation of indoor air and $He-O_2$ gas mixtures were measured and ${\Delta}V_{max50},\;{\Delta}V_{max75},\;V_{iso}V/VC$ between the dust exposed and control workers were compaired. Results: 1) There were no significant differences between two group in ${\Delta}V_{max50}$ and ${\Delta}V_{max75}$. But the ratio of $V_{iso}V/VC$ of the subjects was siginificantly higher than that of the control (p<0.01). This study confirms that $V_{iso}V/VC$ is a very useful index in early detection of small airway dysfunction. 2) The ratio of $V_{iso}V/VC$ of the subjects was signigicantly different between only smoker group and mixed group(smoker and nonsmoker). It suggestes that smoking is an important cousative factor of small airway dysfunction. 3) As the profusion of the chest X-ray increased, the rartio of $V_{iso}V/VC$ increased, but no significant difference of $V_{iso}V/VC$ was found between categories of pneumoconiosis. The categories of pneumoconiosis and small airway dysfunction may not be related. 4) No significant relationship was established between the duration of work and the ratio of $V_{iso}V/VC$. Conclusions : It is concluded that the measurement of $V_{iso}V/VC$ is useful to detect small airway dysfuction of early stage pnuemoconiosis patents with small opacities but showing normal pulmonary function in a routine pulmonary function test.

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Effects of Cow에s Milk Addition on the Quality of Soybean Curd (우유 첨가가 두부 품질에 미치는 영향)

  • 김중만;김형태;최용배;황호선;김태영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.437-442
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    • 1993
  • Soybean curds were made from the mixture of soybean milk and cow's milk (raw milk) at the ratio of 10, 20, 30, 40, 50% (v/v), and then the general composition and mineral content, pH, yield, firmness by sensory evaluation and amino acid composition were investigated. As cow's milk addition increased, total sugar crude fat, crude protein, ash content, firmness, weight and volume of the soybean curd were increased. Whereas moisture content decreased, $Ca^{++}$, $K^{+}$, and Na$^{+}$ were increased, but $Mg^{++}$ and Fe$^{++}$ decreased. In sensory evaluation, color (white), firmness, flavor, and taste were higher than those of control as cow's milk increased. By adding cow's milk at the ratio of 10, 20, 30, 40%, and 50%, sulfur containing amino acid content such as methionine and cystine were enriched 1.31, 1.58, 1.67, 1.85, and 1.95 times, respectively.

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A Study on CdS Deposition using Sputtering (Sputtering을 이용한 CdS 증착에 관한 연구)

  • Lee, Dal-Ho;Park, Jung-Cheul
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.4
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    • pp.293-297
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    • 2020
  • This paper tried to find the best conditions that could be applied to solar cells by deposition of CdS thin film on ITO glass using multiplex displacement sputter system. RF power was changed to 50W, 100W, and 150W and sputtering time was set to 10 minutes. As a result of the measurement of transmittance, the average transmittance in the area of 400 to 800 nm was measured from 60% to 80% and the best characteristic was measured at 150W at 84%. The band gap was also measured at 3.762eV at 50W, 4.037eV at 100W and 4.052eV at 150W. In XRD analysis, even as RF power was increased, it was observed as a structure called Wurtzite (hexagonal) of CdS. And as RF power increased, the particles were large and uniformly deposited, but at 100W the particles were densely composed and dense. And the thickness measurement showed that the RF power increased uniformly.

Evaluating Activation for 50 MeV Cyclotron Irradiation Service using Monte Carlo Method and Inventory Code (50 MeV 사이클로트론 조사 서비스로 인한 방사화 평가)

  • Kim, Sangrok;Kim, Gi-sub;Heo, Jaeseung;Ahn, Yunjin
    • Journal of the Korean Society of Radiology
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    • v.15 no.4
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    • pp.415-427
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    • 2021
  • Korea Institute of Radiological and Medical Sciences has provided various beam irradiation services to researchers using a 50 MeV cyclotron beam line. In particular, since the neutron beam service uses the nuclear reaction between protons and beryllium, the possibility of activation of the irradiated sample increases by using a high current. In this study, MCNP 6.2 and FISPACT-II 4.0 were used to evaluate the possible activation during the 35 MeV 20 ㎂ neutron beam service, which is preferred by the researchers. As a result of the calculation, if the iron, copper, and tungsten samples were irradiated for more than 1 hour, long-lived radioisotopes were produced and their radioactivity exceeded the standard level for self-disposal. Under the conditions of 2 hours of daily irradiation, no activation occurred in the building materials, and the internal exposure of workers due to air activation inside the irradiation room was very insignificant. And when this air was discharged to environment, the radioactivity including this air was also satisfied the emission standard.

On Some New Generalized Di erence Statistically Convergen Sequence Spaces De ned by a Sequence of Orlicz Function

  • Bekt, Cigdem Asma;Atici, Gulcan
    • Kyungpook Mathematical Journal
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    • v.50 no.3
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    • pp.389-397
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    • 2010
  • In this paper we introduce the new generalized difference sequence space $\ell_\infty$($\Delta_v^n$, M,p,q,s), $\bar{c}$($\Delta_v^n$,M,p,q,s), $\bar{c_0}$($\Delta_v^n$,M,p,q,s), m($\Delta_v^n$,M,p,q,s) and $m_0$($\Delta_v^n$,M,p,q,s) defined over a seminormed sequence space (X,q). We study some of it properties, like completeness, solidity, symmetricity etc. We obtain some relations between these spaces as well as prove some inclusion result.

Design of a Low Drop-out Regulator with a UVLO Protection Function (UVLO 보호기능이 추가된 LDO 레귤레이터 설계)

  • Park, Won Kyeong;Lee, Su Jin;Park, Yong Su;Song, Han Jung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.10
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    • pp.239-244
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    • 2013
  • This paper presents a design of the CMOS LDO regulator with a UVLO protection function for a high speed PMIC. Proposed LDO regulator circuit consists of a BGR reference circuit, an error amplifier and a power transistor and so on. UVLO block between the power transistor and the power supply is added for a low input protection function. Also, UVLO block showed normal operation with turn-off voltage of 2.7V and turn-on voltage of 4 V in condition of 5 V power supply. Proposed circuit generated fixed 3.3 V from a supply of 5V. From SPICE simulation results using a $1{\mu}m$ high voltage CMOS technology, simulation results were 5.88 mV/V line regulation and 27.5 uV/mA load regulation with load current 0 mA to 200 mA.

Manufacturing and Physicochemical Properties of Wine using Hardy Kiwi Fruit (Actinidia arguta) (다래를 이용한 발효주의 제조 및 이화학적 특성)

  • Park, Kyung Lok;Hong, Sung Wook;Kim, Young Joon;Kim, Soo Jae;Chung, Kun Sub
    • Microbiology and Biotechnology Letters
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    • v.41 no.3
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    • pp.327-334
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    • 2013
  • For the development of hardy kiwi wine, we arranged for the post-maturity of hardy kiwi fruit, treated them with calcium carbonate and a pectinase enzyme complex, investigated the resulting physicochemical properties and conducted a sensory evaluation. The period determined for creating post-maturity in the hardy kiwi fruit was determined as 5 days storage at room temperature following maturity. During this time the yield of fruit juice was increased from 22.1% to 53.5% using 0.1% (v/v) cytolase PCL5 for 2 h at room temperature. 0.1% (w/v) calcium carbonate was also added during the process of aging, for the reduction of the sour taste. The fermentation trial of the hardy kiwi wine was prepared using water (25% or 50%), sugar ($24^{\circ}brix$), 0.1% (w/v) $CaCO_3$, 0.1% (v/v) cytolase PCL5, $K_2S_2O_5$ (200 ppm), and yeast ($1.5{\times}10^7$ cell/ml). Fermentation then occurred for 2 weeks at $20^{\circ}C$. The pH value, total acidity, alcohol, and reducing sugar content of the resulting hardy kiwi wines of 25% (v/w) and 50% (v/w) water, were in a range of pH 3.4-3.7, 1.12-1.21%, 14.3-14.4%, and 15-16 g/l, respectively. Citric acid and fructose constituted the major organic acids and the free sugar of the 25% and 50% hardy kiwi wine, respectively. Volatile flavor components, including 10 kinds of esters, 8 kinds of alcohols, 5 kinds of acids, 3 kinds of others and aldehydes, were determined by GC analysis. The results of sensory evaluation demonstrated that 50% hardy kiwi wine is more palatable than 25% hardy kiwi wine.

Study on the sexing of preimplantation mouse embryo exposed to H-Y ntisera I. Sexing of mouse embryos by cytolytic assay (H-Y항체에 의한 생쥐초기배의 성판별에 관한 연구 I. 세포발육능검사에 의한 성판별)

  • 양부근;김정익
    • Korean Journal of Animal Reproduction
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    • v.12 no.1
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    • pp.31-36
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    • 1988
  • There studies were conducted using inbred ICR mice to examine the sex of preimplantation mouse embryo. The morphological normality of mice embryos treated with the culture medium containing rat H-Y antiserum(10%, v/v) plus complement(20%,v/v) was observed and also the sexing of embryos was investigated by chromosomal analysis. The results obtained were summarized as follows: 1. The viability of preimplantation mouse embryos, which were incubated in vitro with different media condition, was scored 68.9-85.5% in control group. However, 151 embryos normally developed up to blastocyst and 160 embryos were retarded growth or destroyed out of total 311 embryos treated in the medium containing H-Y antiserum(10%, v/v) plus complement(20%,v/v). 2. H-Y antiserum was prepared from inb red rats (Wistar and Donryu strain) with different immunization times (4, 5 and 6th) to examine the specific titer of embryos by the number of immunization. Precentage of normally developed embryos incubated either in the medium containing the antiserum of Wistar plus complement or Donryu plus complement was revealed 50.9, 47.4 and 50.0% (4, 5 and 6th immunization and 47.8, 41.2 and 48.7%, respectively. 3. Twenty two females and five males were identified out of fourty-eight normally developed embryos incubated in the medium containing H-Y antiserum plus complement by chromosomal analysis.

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STRONG MORI MODULES OVER AN INTEGRAL DOMAIN

  • Chang, Gyu Whan
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.6
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    • pp.1905-1914
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    • 2013
  • Let D be an integral domain with quotient field K, M a torsion-free D-module, X an indeterminate, and $N_v=\{f{\in}D[X]|c(f)_v=D\}$. Let $q(M)=M{\otimes}_D\;K$ and $M_{w_D}$={$x{\in}q(M)|xJ{\subseteq}M$ for a nonzero finitely generated ideal J of D with $J_v$ = D}. In this paper, we show that $M_{w_D}=M[X]_{N_v}{\cap}q(M)$ and $(M[X])_{w_{D[X]}}{\cap}q(M)[X]=M_{w_D}[X]=M[X]_{N_v}{\cap}q(M)[X]$. Using these results, we prove that M is a strong Mori D-module if and only if M[X] is a strong Mori D[X]-module if and only if $M[X]_{N_v}$ is a Noetherian $D[X]_{N_v}$-module. This is a generalization of the fact that D is a strong Mori domain if and only if D[X] is a strong Mori domain if and only if $D[X]_{N_v}$ is a Noetherian domain.