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Modification and Validation of an Analytical Method for Dieckol in Ecklonia Stolonifera Extract (곰피추출물의 지표성분 Dieckol의 분석법 개선 및 검증)

  • Han, Xionggao;Choi, Sun-Il;Men, Xiao;Lee, Se-jeong;Oh, Geon;Jin, Heegu;Oh, Hyun-Ji;Kim, Eunjin;Kim, Jongwook;Lee, Boo-Yong;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.37 no.3
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    • pp.143-148
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    • 2022
  • This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography-photodiode array. The results showed that the correlation coefficient of calibration curve (R2) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 ㎍/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91-102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.

A Longitudinal Validation Study of the Korean Version of PCL-5(Post-traumatic Stress Disorder Checklist for DSM-5) (PCL-5(DSM-5 기준 외상 후 스트레스 장애 체크리스트) 한국판 종단 타당화 연구)

  • Lee, DongHun;Lee, DeokHee;Kim, SungHyun;Jung, DaSong
    • Korean Journal of Culture and Social Issue
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    • v.28 no.2
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    • pp.187-217
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    • 2022
  • The aim of this study is to examine the psychometric properties of the Korean version of the Post-traumatic Stress Disorder Checklist for DSM-5(PCL-5). For this purpose, online surveys were conducted for two times with a one year interval using the data from 1,077 Korean adults at time 1, and 563 Korean adults at time 2. First, from the result of the confirmatory factor analysis, comparing the model fit of the 1, 4, 6, and 7-factor model, the 4, 6, and 7-factor model showed a acceptable fit, and the best fit was seen in the order of the 7, 6, 4-factor model. Second, the internal consistency, omega coefficient, construct validity, average variance extracted, and test-retest reliability results were all satisfactory.. Third, a correlation analysis with the K-PC-PTSD-5 and the sub-factors of BSI-18 was conducted to check the validity of the Korean Version of PCL-5. As a result, a positive correlation was seen with both K-PC-PTSD-5 and BSI-18. Fourth, a hierarchical multiple regression was performed to examine whether the Korean Version of PCL-5 predicts future PTSD, depression, anxiety, and somatization. As a result, the Korean Version of PCL-5 measured at time 1 significantly predicted PTSD, depression, anxiety, and somatization symptoms at time 2. Fifth, by analyzing the ROC curve, the discriminant power of PCL-5 for screening PTSD symptom groups was confirmed, and the best cut-off score was suggested. As a result of the longitudinal validation of Korean version of PCL-5, it was found that this scale is a reliable and valid measure for Korean adults. By looking into the predictive validity of the scale, it was found that the Korean version of PCL-5 can predict not only PTSD symptoms but also PTSD-related symptoms such as depression, anxiety, and somatization. Also, this study differs from previous validation studies measuring PTSD symptoms in that it suggested a cut-off score to help differentiate PTSD symptom groups.

A Safety Survey of Pesticide Residues on Agricultural Products Marketed in Incheon from 2019 to 2021 (인천광역시 유통 농산물의 잔류농약 안전성 조사)

  • Park, Byung-Kyu;Kwon, Sung-Hee;Yeom, Mi-Sook;Han, Se-Youn;Kang, Min-Jung;Joo, Kwang-Sig;Heo, Myung-Je;Kwon, Mun-Ju
    • Journal of Food Hygiene and Safety
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    • v.37 no.4
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    • pp.249-259
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    • 2022
  • This study investigated pesticide residues in 7,069 agricultural products distributed in the city of Incheon from 2019 to 2021. Ingestion of pesticides can cause serious carcinogenic, endocrine, neurological, and reproductive problems. Pesticide residues in the samples were analyzed using various multi-residue methods (GC-MS/MS, GC-ECD/NPD, LC-MS/MS, and HPLC-UVD) on the Korean Food Code. The violation rate of the samples exceeding the maximum residue level (MRL) of pesticide residues for the years 2019, 2020, and 2021 was 1.0%, 1.4%, and 1.1%, respectively. Diazinon, flubendiamide, procymidone, fluxametamide, and fluquinconazole were the most frequently reported violative pesticide residues. Most commonly encountered agricultural products exceeding MRLs were coriander leaves, chamnamul, chwinamul, welsh onion, and crown daisy. Agricultural products and pesticides frequently exceeding MRLs should be continuously inspected for food safety. Continuous monitoring of pesticide residues in agricultural products is indispensable to improve consumer safety by preventing the distribution of agricultural products exceeding MRLs.

Effects of Dry Heat Treatment on the Reduction of Main Food-Borne Bacteria on Alfalfa Seeds (건열처리를 이용한 알팔파의 주요 식중독균 저감화)

  • Hong, Soon-Young;Kim, Su-jin;Bang, Woo-Suk
    • Journal of Food Hygiene and Safety
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    • v.37 no.4
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    • pp.225-231
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    • 2022
  • In this study, the conditions of dry heat treatment (21 days at 65℃, 16 days at 70℃, 10 days at 75℃, and 7 days at 80℃) were investigated to inactivate Bacillus cereus ATCC 12480, Listeria monocytogenes ATCC SSA81, Staphylococcus aureus ATCC 6538, Escherichia coli O157:H7 ATCC 43894, and Salmonella Typhimurium ATCC 14028 on alfalfa seeds, without affecting the rate of germination of seeds. Alfalfa seeds were inoculated at levels of 6-7 log CFU/g and treated with dry heat at 65℃, 70℃, 75℃, and 80℃; thereafter, the rate of seed germination was determined. The rate of germination was set at 70%, according to the market standards. The bacteria were inactivated when B. cereus was treated with dry heat for 21 days at 65℃, 18 days at 70℃, 14 days at 75℃, and 4 days at 80℃; L. monocytogenes was treated for 21 days at 65℃, 18 days at 70℃, 12 days at 75℃, and 7 days at 80℃; S. aureus was treated for 18 days at 65℃, 18 days at 70℃, 11 days at 75℃, and 4 days at 80℃; E. coli O157:H7 was treated for 21 days at 65℃, 18 days at 70℃, 12 days at 75℃, and 6 days at 80℃; and Sal. Typhimurium was treated for 24 days at 65℃, 22 days at 70℃, 14 days at 75℃, and 7 days at 80℃. For all bacteria, the D-value (R2 = 0.5656-0.7957) significantly decreased when the temperature increased from 65℃ to 80℃ (P<0.05). Since dry heat treatment of alfalfa seeds at 80℃ for 7 days affects their germination rate, dry heat treatment at 75℃ for 14 days is the most effective way to ensure their safety. This study suggests a potential method of bacterial inactivation using dry heat treatment to increase the microbiological safety of sprouts.

Evaluation and Physicochemical Property for Building Materials from the Japanese Ministry of General Affairs in Joseon Dynasty (일제강점기 조선통감부 건축재료의 물리화학적 특성과 평가)

  • Park, Seok Tae;Lee, Jeongeun;Lee, Chan Hee
    • Economic and Environmental Geology
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    • v.55 no.4
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    • pp.317-338
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    • 2022
  • Physicochemical characteristics and evaluation were studied by subdividing the concretes, bricks and earth pipes on the site of the Japanese Ministry of General Affairs in Joseon Dynasty, known as modern architecture, into three periods. Concretes showed similar specific gravity and absorption ratio, and large amounts of aggregates, quartz, feldspar, calcite and portlandite were detected. Porosity of the 1907 bricks were higher than those of 1910 and 1950 bricks. All earthen pipe is similar, but the earlier one was found to be more dense. Bricks and earthen pipes are dark red to brown in color within many cracks and pores, but the matrix of the earthen pipe is relatively homogeneous. Quartz, feldspar and hematite are detected in bricks, and mullite is confirmed with quartz and feldspar in earthen pipes, so it is interpreted that the materials have a firing temperature about 1,000 to 1,100℃. Concretes showed similar CaO content, but brick and earthen pipe had low SiO2 and high Al2O3 in the 1907 specimen. However, the materials have high genetic homogeneity based on similar geochemical behaviors. Ultrasonic velocity and rebound hardness of the concrete foundation differed due to the residual state, but indicated relatively weak physical properties. Converting the unconfined compressive strength, the 1st extended area had the highest mean values of 45.30 and 46.33 kgf/cm2, and the 2nd extended area showed the lowest mean values (20.05 and 24.76 kgf/cm2). In particular, the low CaO content and absorption ratio, the higher ultrasonic velocity and rebound hardness. It seems that the concrete used in the constructions of the Japanese Ministry of General Affairs in Joseon Dynasty had similar mixing characteristics and relatively constant specifications for each year. It is interpreted that the bricks and earthen pipes were through a similar manufacturing process using almost the same raw materials.

Exploring the contextual factors of episodic memory: dissociating distinct social, behavioral, and intentional episodic encoding from spatio-temporal contexts based on medial temporal lobe-cortical networks (일화기억을 구성하는 맥락 요소에 대한 탐구: 시공간적 맥락과 구분되는 사회적, 행동적, 의도적 맥락의 내측두엽-대뇌피질 네트워크 특징을 중심으로)

  • Park, Jonghyun;Nah, Yoonjin;Yu, Sumin;Lee, Seung-Koo;Han, Sanghoon
    • Korean Journal of Cognitive Science
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    • v.33 no.2
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    • pp.109-133
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    • 2022
  • Episodic memory consists of a core event and the associated contexts. Although the role of the hippocampus and its neighboring regions in contextual representations during encoding has become increasingly evident, it remains unclear how these regions handle various context-specific information other than spatio-temporal contexts. Using high-resolution functional MRI, we explored the patterns of the medial temporal lobe (MTL) and cortical regions' involvement during the encoding of various types of contextual information (i.e., journalism principle 5W1H): "Who did it?," "Why did it happen?," "What happened?," "When did it happen?," "Where did it happen?," and "How did it happen?" Participants answered six different contextual questions while looking at simple experimental events consisting of two faces with one object on the screen. The MTL was divided to sub-regions by hierarchical clustering from resting-state data. General linear model analyses revealed a stronger activation of MTL sub-regions, the prefrontal lobe (PFC), and the inferior parietal lobule (IPL) during social (Who), behavioral (How), and intentional (Why) contextual processing when compared with spatio-temporal (Where/When) contextual processing. To further investigate the functional networks involved in contextual encoding dissociation, a multivariate pattern analysis was conducted with features selected as the task-based connectivity links between the hippocampal subfields and PFC/IPL. Each social, behavioral, and intentional contextual processing was individually and successfully classified from spatio-temporal contextual processing, respectively. Thus, specific contexts in episodic memory, namely social, behavior, and intention, involve distinct functional connectivity patterns that are distinct from those for spatio-temporal contextual memory.

Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

A Study on Status of Landscape Architecture Industry with National Statistics (국가통계자료를 활용한 조경산업 현황 연구)

  • Choi, Ja-Ho;Yoon, Young-Kwan;Koo, Bon-Hak
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.5
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    • pp.40-53
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    • 2022
  • This study carried out to provide the methodology and basic status material of using Korean national statistics needed to find the actual state of the landscape architecture industry. The landscape architecture industry was classified into 'Design', 'Construction Management', 'construction', 'Maintenance & Management', 'Materials', 'Research', 'Education', and 'Administration' areas. In each field, business types were systemized and associated in accordance with Korean standard industrial classification and legislations pertinent to construction. Among them, the business types directly defined in the construction related legislations under the Ministry of Land, Infrastructure and Transport were focused on, and the establishment, association, integration, distribution, duplication, and omission of national statistics were analyzed. As a result, the business types of statistical analysis were selected. In order for commonality of statistical items and minimized error of interpretation, semantic analysis was conducted. Finally, the number of registered business types, the number of workers, and sales were selected. Based on them, the analysis framework applicable to fundamental analysis and evaluation of the actual state of the industry was proposed. Actual national statical data were applied for analysis and evaluation. In 2019, the number of registered business types related to the landscape architecture industry was 12,160, the number of workers by business type was 106,296, and the sales by business type were 8,308.5 billion KRW. The number of registered business types and the number of workers had been on the rise from 2017, whereas the sales had been on the decrease. It is required to come up with a plan for industrial development. This study was conducted with the national statistics established by multiple public institutions, so that there are limitations in securing consistency and reliability. Therefore, it is necessary to establish systematic and consistent national statistics in accordance with 「Landscaping Promotion Act」. In the future, it will planned to research application and development plans of national statistics according to subjects including park and green.

An analysis of customer needs for the operation of unmanned food stores on a university campus (대학 캠퍼스 내 무인 식품 매점 운영에 대한 고객 요구도 분석)

  • Kim, Se-Eun;Park, Min-Seo;Bae, Hyun-Joo
    • Journal of Nutrition and Health
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    • v.55 no.5
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    • pp.587-600
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    • 2022
  • Purpose: This study was performed to analyze customer needs for the operation of unmanned food stores on a university campus. Methods: A total of 329 responses were collected from customers who had purchased food at the stores. Statistical analyses were conducted using the SPSS program for frequency analysis, χ2-test, t-test, one-way analysis of variance, and Duncan's multiple range test. Results: On average, the overall satisfaction score for lunch and the eating location was 3.91 out of 5 points. Overall satisfaction was significantly higher for home cooking, and lower for eating at convenience stores (p < 0.001), and the score for eating with family was significantly higher than for eating alone or with friends (p < 0.001). On average, the intention to use an unmanned store received a score of 3.98 out of 5 points, while 'home cooking (3.76)' was significantly lower than other eating locations and eating at 'convenience stores (4.38)' was significantly higher than other eating locations (p < 0.05). The desired time of use for unmanned food stores was 'lunch (54.1%)', 'dinner (46.2%)', and 'afternoon snack (41.9%)', and the desired foods were 'doshirak (62.0%)', 'sandwich (56.2%)', 'fruit cup (54.4%)', 'salad (51.7%)', and 'kimbap (50.5%)'. There were significantly more opinions that single-person households would use unmanned food stores for dinner more than family households (p < 0.05), and significantly more willingness to purchase fruit cup (p < 0.05). The average prices (Korean won) that the subjects were willing to pay for the purchase of food were 4,991 for doshirak, 3,403 for salad, 2,745 for fruit cup, 2,609 for sandwich, and 2,235 for kimbap. Conclusion: For the successful operation of the unmanned food stores on the university campus, the store manager should grasp the customer's needs in real-time and establish an effective marketing strategy.

Report on Extended Leak-Off Test Conducted During Drilling Large Diameter Borehole (국내 대구경 시추공 굴진 중 Extended Leak-Off Test 수행 사례 보고)

  • Jo, Yeonguk;Song, Yoonho;Park, Sehyeok;Kim, Myung Sun;Park, In-Hwa;Lee, Changhyun
    • Tunnel and Underground Space
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    • v.32 no.5
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    • pp.285-297
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    • 2022
  • We report results of Extended Leak-Off Test (XLOT) conducted in a large diameter borehole, which is drilled for installation of deep borehole geophysical monitoring system to monitor micro-earthquakes and fault behavior of major fault zones in the southeastern Korean Peninsula. The borehole was planned to secure a final diameter of 200 mm (or more) at a depth of ~1 km, with 12" diameter wellbore to intermediate depths, and 7-7/8" (~200 mm) to the bottom hole depth. We drilled first the 12" borehole to approximately 504 m deep and installed American Petroleum Institute standard 8-5/8" casing, then annulus between the casing and bedrock was fully cemented. XLOT was carried out for several purposes such as confirming casing and cementing integrity, measuring rock stress states. To that end, we drilled additional 4 m long open hole interval to directly inject water and pressurize into the rock mass using the upper API casings. During the XLOT, flow rates and interval pressures were recorded in real time. Based on the logs we tried to analyze hydraulic conductivity of the test interval.